Studies have been conducted on the mean radiant temperature (Tmrt) estimation methods using globe thermometers for measuring outdoor thermal environments in various climate regions. Yet, given the unique thermal environments of semi-outdoor spaces, these Tmrt estimation methods may not be appropriate for the spaces with shading effects. This study aims to assess the thermometric and radiative methods for Tmrt estimation in semi-outdoor spaces in humid sub-tropical climates, taking data from six-directional radiation measurements as validations. During the typical summer and winter days in Wuhan, China, the measurements were carried out in semi-outdoor spaces facing south and west, with an open square for comparison, including air temperature, humidity, wind velocity, black-globe temperature, and long- and short-wavelength radiation fluxes obtained from four-component net radiometers. It was found the maximum mean deviation of diurnal Tmrt values estimated by the black-globe thermometer method in semi-outdoor spaces was 7.1 °C, which exceeded the threshold required by ISO7726, where the shortwave radiation was regarded as the dominant interference factor. To improve the accuracy and cost-effectiveness of Tmrt estimation, we proposed linear empirical equations to calibrate the Tmrt estimated by black-global thermometer method that the root mean square error (RMSE) of the Tmrt deviations can generally vary from 2.22 to 2.72 °C. Contributing to the thermal comfort research, this study proposed an empirical method for estimating Tmrt in semi-outdoor spaces in humid subtropical climates, which can generate satisfactory results in accordance with ISO7726 standards with cost-effective instrumentation.