A new biostratigraphic biozonation for the Paleocene to Late Oligocene succession of the onshore Niger Delta has been developed based on the identification of significant stratigraphic dinoflagellate cyst events. This study is aimed at applying rarely used dinoflagellate cysts events in dating, correlating and inferring the paleoenvironment of penetrated sedimentary successions in the studied area. Palynological processing and analysis were carried out on two hundred and twenty ditch cutting samples recovered from two exploration wells (BN-1 and FJ-1) located in the Greater Ughelli and Northern Depobelts of the onshore Niger Delta respectively. Abundant and diverse dinoflagellate cysts recovered included marker Peridinales species Apectodinium sp., Palaeocystodinium australinum, and Gonyaulacales such as Areosphaeridium arcuatum, Cordosphaeridium gracile, Tuberculodinium vancampoae, Polysphaeridium zoharyi and Hystrichokolpoma rigaudiae. Three dinoflagellate cysts Palynological Zones - DPZ 100 (Early to Late Paleocene), DPZ 200 (Early to Late Eocene) and DPZ 300 (Late Eocene to Late Oligocene) are proposed herein. The biozones were subdivided into ten subzones based on the Top occurrence, Quantitative Tops and Quantitative base as well as ACME events of dinoflagellate cysts marker taxa which are used to date the rock sequence to be Paleocene to Late Oligocene. Abundant and diverse shallow outer neritic to inner neritic dinoflagellate cysts are identified from thick grey soft shales at the lower section of the studied wells. The upper section show significant reduction in dinocysts assemblage dominated by inner (coastal) neritic taxa. This dinocysts occurrence and distribution suggests inner (coastal) neritic paleoenvironment at the upper sections which deepened into shallow outer neritic conditions at the lower intervals of the studied wells. The thick brown to grey shales at the lower section and the medium to fine grained, moderately well sorted sandstone interbeds at the middle section of the studied wells are interpreted to be potential source and reservoir rocks respectively. These datasets are useful for correlating possible hydrocarbon source rocks and potential reservoir carrier beds in the Gulf of Guinea.