Neotropical Species Research Articles

Assessing the role of taxonomic impediment in the knowledge on geographic distribution of Physidae (Gastropoda: Hygrophila) in South America, using an intensive-data approach.

There is a marked disparity in the state of knowledge of Holartic x Neotropical species of the freshwater snail family Physidae; the incipiency of data on Neotropical physids reflecting the lower number of dedicated specialists. The gaps in the knowledge on Neotropical physids have led to historical uncertainty about species validity. Revisiting the species is essential to reduce taxonomic impediment and delineating their probable distribution is the first step to attain this purpose. We aimed at critically analyze occurrence records of South American physids, compiled through an intensive search in the literature, biodiversity and molecular databases. We present a provisional characterization of the distribution of this family in South America, considering the probable versus the poorly documented distribution of the species. The critical underrepresentation of South American physids in collections, molecular databases and literature reinforces the role of taxonomic impediment in delaying the advance of the knowledge on species diversity. Malacological collections represented the main source of records, evidencing the relevance of unpublished data associated to specimens to assess distributional information on neglected groups. As most of the species are represented by shells, the reassessment of species identity and distribution must be done, using molecular and anatomical criteria for species delimitation.

New species and records of Palaearctic, Afrotropical and Neotropical ichneumon wasps (Hymenoptera: Ichneumonidae)

Palaearctic, Afrotropical and Neotropical species of the subfamilies Campopleginae, Acaenitinae, Cryptinae, Ophioninae and Tryphoninae (Hymenoptera: Ichneumonidae) are treated. Regarding Campopleginae, two new species are described, namely Hyposoter daeva sp. nov. from Iran and Nemeritis centurio sp. nov. from Argentina, and the first records of Bathyplectes incisus Horstmann, 1974 and B. quinqueangularis (Ratzeburg, 1852) from Ukraine, Campoletis yaga Vas, 2024 from Argentina, Casinaria pyreneator Aubert, 1960, Diadegma compunctellae Horstmann, 2013, D. longicaudatum Horstmann, 1969, D. nigrostigmaticum Horstmann, 1969 and D. semiclausum (Hellén, 1949) from Hungary, Hyposoter caedator (Gravenhorst, 1829) and H. didymator (Thunberg, 1822) from Malta, and H. orbator (Gravenhorst, 1829) from Iran are reported. Regarding Acaenitinae, Hieroceryx pseudoglomiger Benoit, 1951 is reported for the first time from Ghana. Regarding Cryptinae, Gabunia coerulea Kriechbaumer, 1895 is also reported for the first time from Ghana. Regarding Ophioninae, Enicospilus babaulti (Seyrig, 1935) and E. biimpressus (Brullé, 1846) are reported for the first time from Guinea, and Thyreodon boliviae Morley, 1912 is reported for the first time from French Guiana. Regarding Tryphoninae, Netelia caucasica (Kokujev, 1899) is reported for the first time from Hungary. With four figures.

Chalarus (Diptera: Pipunculidae) of Colombia, with description of two new species and an updated key to males of the Neotropical species

Chalarus Walker, 1834 (Diptera: Pipunculidae: Chalarinae) has a cosmopolitan distribution, with 11 known species in the Neotropical Region, but none recorded in Colombia. Two new species of Chalarus are described from Colombia, namely Chalarus boyacensis sp. nov. (type locality: Cabaña Chaina, Santuario de Fauna y Flora Iguaque) and C. chairensis sp. nov. (type locality: Vereda Tigrera Alta, Cartagena del Chaira). Chalarus absonus Rafael, 1990, C. delicatus Rafael, 1990 and C. connexus Rafael, 1988 are recorded in Colombia for the first time, with amended and comparative diagnoses. Photographs of five South American species, distribution map and a dichotomic key to males of all Neotropical species are included.

Pipunculus Latreille, 1802 (Diptera: Pipunculidae) of Colombia, with description of three new species and an updated key to males of the Neotropical species.

Pipunculus Latreille, 1802 is a cosmopolitan genus of big-headed flies (Pipunculidae), with nine known species in the Neotropical region, but completely unknown in Colombia. Three new species of Pipunculus are described from Colombia, namely Pipunculus caeruleus sp. nov. (type-locality: Pramo de Santa Ins, Belmira), P. chiminiguagua sp. nov. (type-locality: Cabaa Chaina, Santuario de Fauna y Flora Iguaque) and P. planus sp. nov. (type-locality: Los Mudos, Santuario de Fauna y Flora Iguaque). Illustrations and a new dichotomic key to the Neotropical species are presented. This paper increases the number of Neotropical Pipunculus to 11, five in South America and three in Colombia, which is now the South American country with the highest diversity of this genus.

Different response of females and males Neotropical catfish (Rhamdia quelen) upon short-term temperature increase.

Climate change has been one of the most discussed topics in the world. Global warming is characterized by an increase in global temperature, also in aquatic environments. The increased temperature can affect aquatic organisms with lethal and sublethal effects. Thus, it is necessary to understand how different species respond to temperature. This study aimed to evaluate how the Neotropical catfish species Rhamdia quelen responds to temperature increases. The fish were exposed to temperatures of 25°C (control) and 30°C after gradual temperature increase for 7days. After 96h in each temperature, the fish were anesthetized, blood was collected, and after euthanasia, brain, liver, posterior kidney, gills, muscle, and gonads were collected. The gonads were used for sexing, while other tissues were used for the hematological, biochemical, genotoxic, and histopathological biomarkers analysis. Hepatic proteomic analysis with a focus on energy production was also carried out. Blood parameter changes in both sexes, including an increase in glucose in males, leukopenia in females, and genotoxicity in both sexes. Hepatic proteins related to energy production were altered in both sexes, but mainly in males. Others biomarker alterations, such as histopathological, were not observed in other tissues; however, the antioxidant system was affected differently between sexes. These showed that R. quelen juveniles, at temperatures higher than its optimum temperature such as 30°C, has several sublethal changes, such as hematological alterations, antioxidant system activation, and energetic metabolism alteration, especially in males. Thus, short-term temperature rise can affect females and males of R. quelen differently.

Two new species of Caryodaphnopsis (Lauraceae) from the Magdalena Medio, Colombia, with an updated key for the Neotropical Caryodaphnopsis species

Background and Aims: Caryodaphnopsis is a tropical genus with an amphi-Pacific distribution, comprising 19 species, 10 of which Neotropical. Two new species are described from wet forest remnants on the western slopes of the Eastern Cordillera of Colombia, which is part of the Magdalena Medio biogeographical region. Their morphological relationships with Neotropical allied species are discussed.Methods: Descriptions and illustrations of both new species were based on collections from the Santander and Boyacá departments, deposited in the herbaria of the Bogotá Botanical Garden (JBB) and the Universidad Industrial de Santander (UIS). Measurements of vegetative parts and inflorescences were taken from herbarium material. Floral structures and fruits were measured from fresh material preserved in alcohol. Threat categories were proposed according to IUCN criteria.Key results: Caryodaphnopsis carmensis and C. yariguiensis are two species so far known only from the Magdalena Medio, Colombia. Caryodaphnopsis carmensis is unique because of its dense, golden pubescence on vegetative parts, leaves with subcordate base and 14-17 arcuate tertiary veins, located between the first pair of secondary veins. Caryodaphnopsis yariguiensis resembles C. fieldii but differs from the latter by the shape of leaves and the stamens with a pair of glands located between the middle portion of filament. Both taxa are proposed as Endangered (EN). An updated key for the Neotropical Caryodaphnopsis species is presented, along with information about their geographic distribution. Conclusions: The two species described are trees that yield one of the finest timbers in the Magdalena Medio and their populations are much reduced. We encourage their cultivation to promote their conservation. With these two new species, Colombia is the country with the greatest diversity of the genus (seven) and the Magdalena Medio has the highest concentration of endemic species (three).

A New Eyeless Species of Micranops Cameron 1913 from Bolivia (Coleoptera: Staphylinidae: Paederinae)

This work adds a new eyeless species of the genus Micranops Cameron, 1913 (Staphylinidae, Paederinae, Scopaeina) to the endogean Coleoptera of the Bolivian fauna. The loss of eyes is usually associated with adaptations to subterranean habitats, although M. bolivianus Guzman & Żyła sp. nov. was collected from leaf litter. We also report new occurrences for the genus west of the Andes mountain range. Our work increases the number of known Neotropical Micranops species to seven and extends the known distribution range for the genus in South America.

Diversity of Neotropical stalked-puffball: Two new species of Tulostoma with reticulated spores

Species of the genus Tulostoma are easily recognizable by the presence of a spore sac, with a mouth from which spores are released, attached to a stipe. Tulostoma is a species-diverse genus with a worldwide distribution, and some attempts were made to delimitate species and to evaluate reliable taxonomic-informative characteristics for species identification. However, there is a notable information gap regarding Neotropical species, especially for geographic distribution and DNA data, which hampers further understanding of the infrageneric diversity, evolution, and ecology of this genus. Based on morphological analysis, molecular phylogenetics and geographic distribution, we propose here two new species of Tulostoma with reticulated spores, from the two threatened Brazilian geographical areas, Atlantic Forest and “campos rupestres” (rupestrian grassland), as well as we provide notes on the taxonomic rank of Tulostoma exasperatum var. ridleyi.

New sand fly (Diptera, Psychodidae) records and COI DNA barcodes in the state of Maranhão, Eastern Amazon, Brazil

The sand fly fauna and the usefulness of the DNA barcoding fragment of the cytochrome c oxidase subunit I (COI) gene were accessed in a forest fragment in the municipality of Governador Newton Bello, state of Maranhão, Brazil. We performed entomological collections in three independent campaigns in May and October 2021, and January 2023. Sand flies were morphologically-identified and then DNA barcoded. Sequences were deposited and analyzed in the BOLD System Database, and various species delimitation algorithms, to assess whether DNA sequences merge into taxonomic units in accordance with nominal species. In total, 1,524 sand flies were collected, comprising 32 nominal species. Nyssomyia antunesi was the most abundant species (31.5 %), followed by Psychodopygus davisi (27 %). We reported for the first time in the state of Maranhão, the presence of Lutzomyia evangelistai, Lutzomyia sherlocki, Pressatia equatorialis, and Psathyromyia barrettoi. We amplified and analyzed 67 COI barcodes of 23 species, which were merged with conspecific sequences extracted from GenBank. The maximum intraspecific p distances ranged from 0.0 % to 14.74 %, while the distances to the nearest neighbor varied from 1.67 % to 13.64 %. The phylogenetic gene tree and species delimitation tools clustered sequences into well-supported clades/clusters for each nominal species, except for Pressatia choti/Pr. equatorialis, which have the lowest interspecific genetic distance (1.67 %). We sequenced for the first time COI barcodes of Brumptomyia brumpti, Evandromyia monstruosa, Micropygomyia rorotaensis, Micropygomyia pilosa, Pintomyia christenseni, Pintomyia pacae, Pr. equatorialis, Pa. barrettoi, and Psathyromyia hermanlenti, which will be useful for further molecular identification and classification proposals of Neotropical species. This study updated the current list of the sand fly fauna for the state of Maranhão to 97, and demonstrated that COI barcodes are useful for specific identification.

Traceability of primordial germ cells in three neotropical fish species aiming genetic conservation actions.

Primordial germ cells (PGCs) are embryonic pluripotent cells that can differentiate into spermatogonia and oogonia, and therefore, PGCs are a genetic source for germplasm conservation through cryobanking and the generation of germline chimeras. The knowledge of PGC migration routes is essential for transplantation studies. In this work, the mRNA synthesized from the ddx4 3'UTR sequence of Pseudopimelodus mangurus, in fusion with gfp or dsred, was microinjected into zygotes of three neotropical species (P. mangurus, Astyanax altiparanae, and Prochilodus lineatus) for PGC labeling. Visualization of labeled PGCs was achieved by fluorescence microscopy during embryonic development. In addition, ddx4 and dnd1 expressions were evaluated during embryonic development, larvae, and adult tissues of P. mangurus, to validate their use as a PGC marker. As a result, the effective identification of presumptive PGCs was obtained. DsRed-positive PGC of P. mangurus was observed in the hatching stage, GFP-positive PGC of A. altiparanae in the gastrula stage, and GFP-positive PGCs from P. lineatus were identified at the segmentation stage, with representative labeling percentages of 29% and 16% in A. altiparanae and P. lineatus, respectively. The expression of ddx4 and dnd1 of P. mangurus confirmed the specificity of these genes in germ cells. These results point to the functionality of the P. mangurus ddx4 3'UTR sequence as a PGC marker, demonstrating that PGC labeling was more efficient in A. altiparanae and P. lineatus. The procedures used to identify PGCs in P. mangurus consolidate the first step for generating germinal chimeras as a conservation action of P. mangurus.

Ruehssia bahiensis: a new species of Apocynaceae endemic to a floristically neglected area within the Semi-arid of Bahia, Brazil

Ruehssia belongs to the Apocynaceae, tribe Marsdeniae, including all neotropical species of this family with erect pollinia. This genus includes about 110 species, 44 of which occur in Brazil. In recent floristic surveys conducted within the Semi-arid region of Bahia state, Northeast Brazil, a new species of Ruehssia was recognised, which is herein described and illustrated. It resembles R. pickelii (Fontella & Morillo) F.Esp.Santo & Rapini, known from the caatinga vegetation in Pernambuco, Paraíba and Rio Grande do Norte states, but has the corolla lobes adaxially pubescent (vs glabrous), corona lobes shorter than (vs as high as) the gynostegium and an exserted, conical (vs inserted and subglobose) style-head apex. The newfound species is named R. bahiensis and is known from only three locations, two within the “sisal territory” and one in the region of Feira de Santana. All these locales are floristically underexplored and have undergone an extensive transformation into pasture and agricultural land.

Taxonomic status of Myotis extremus (Chiroptera, Vespertilionidae) from Mesoamerica, with comments on the distribution and systematics of Myotis nigricans

Myotis nigricans was previously considered the most widely distributed Neotropical Myotis species, occurring from Mexico to northern Argentina, and several allopatric subspecies have been recognized, including M. n. extremus, from Mexico and Guatemala. However, recent studies have shown that M. nigricans is a species complex—many cryptic. Using molecular and morphological data, we assessed the taxonomic status of M. nigricans populations from Mexico, Guatemala, Paraguayan Chaco, and Brazilian Atlantic Forest. Our phylogenetic analysis indicated that M. nigricans is polyphyletic, and the populations classified as M. n. extremus were recovered in a clade composed of species from the ruber group. Populations from Paraguay and Brazil were recovered in distinct clades within the albescens group. Our morphological and morphometric analyzes corroborate the molecular findings, supporting the recognition of M. extremus as a full species. Furthermore, we propose that M. nigricans is a monotypic species occurring exclusively in the South American Atlantic Forest. Populations from other ecoregions should have their taxonomic status redefined in future studies.

Intra-annual stable isotopes in the tree rings of Hymenaea courbaril as a proxy for hydroclimate variations in southern Amazonia

The hydrology of Amazonia is changing due to climate and land-use changes, especially in the southern region, which has warmed and dried faster than other tropical regions. Yet there are no long-term hydrological records to put these changes in a historical perspective. Here we investigate the use of tree-ring carbon (δ13C) and oxygen isotopes (δ18O) to assess the seasonal variation in climate for the southern Amazonia basin. We analysed the intra-annual variation of δ13C and δ18O in 10 segments of each tree ring from 2013 to 2017 from individuals of Hymenaea courbaril, a long-lived and widespread neotropical tree species. We find strong seasonal patterns of tree-ring δ13C supporting previous observations of annual growth rhythms for this species. The intra-annual variation in δ18O shows that the lowest values generally occur just after the middle point of ring formation, corresponding to the peak rainy season. We find strong correlations between the δ18O in the middle of the growth ring and vapour pressure deficit (r = 0.92, P = 0.02) and precipitation (r = −0.93, P = 0.02). We further find associations between the oxygen isotopic series and the discharge of the Araguaia basin’s main rivers during the rainy period. Our results show that these δ18O records are sensitive to fluctuations in rainfall and humidity, and thus to river discharge in the region. Longer reconstructions of based on tree-ring δ18O of Hymenaea courbaril could provide a novel proxy to assess past hydrological changes.

Description of a new species of Deinodryinus from Ecuador: D. hubeni sp. nov. (Hymenoptera, Dryinidae).

A new species of Deinodryinus Perkins, 1907, D. hubeni sp. nov., is described from Ecuador, Tungurahua Province. The new species can be recognized by having the antennal setae shorter than breadth of antennomeres, the face completely sculptured by subparallel longitudinal keels, the paramere with distal extremity covered with sensorial lamellae and with inner proximal branch covered with lamellae and wrapping penis. The male holotype is compared with the known males of all other Neotropical species of the same genus. The key to the Neotropical males of Deinodryinus is modified to include this new species.

Evolution of Seed Dispersal Modes in the Orchidaceae: Has the Vanilla Mystery Been Solved?

Orchid seeds are predominantly wind-dispersed, often developed within dry, dehiscent fruits that typically release millions of dust-like seeds into the air. Animal-mediated seed dispersal is a lesser-known phenomenon in the family and predominantly occurs in groups belonging to early-diverging lineages bearing indehiscent, fleshy fruits with hard, rounded, dark seeds. In this review, we explore the evolutionary trends of seed dispersal mechanisms in Orchidaceae, focusing on the pantropical genus Vanilla. Notably, certain Neotropical species of Vanilla produce vanillin-aromatic compounds synthesized naturally in their fruits, which plays a pivotal role in seed dispersal. Ectozoochory occurs in dry, dehiscent fruits, whose seeds are dispersed by (i) male euglossine bees collecting the fruit’s vanillin aromatic compounds and (ii) female stingless bees collecting the fruit’s mesocarp. Endozoochory occurs in (iii) highly nutritious, indehiscent fruits consumed by terrestrial mammals or (iv) fleshy, dehiscent fruits whose mesocarp is consumed by arboreal mammals. Wind dispersal appears to be a derived state in Orchidaceae and, given its predominance, a trait likely associated with enhanced speciation rates. Zoochory primarily occurs in groups derived from early-diverging lineages; occasional reversions suggest a link between dispersal mode and fruit and seed traits. Interestingly, fruit dehiscence and fleshiness in Vanilla lack phylogenetic signal despite their role in determining dispersal modes, suggesting potential environmental adaptability.

Right or left-handed: are locomotion type, body size, and microhabitat related to forelimb laterality in anurans?

Laterality, the tendency of animals to use the structures on one side of the body more frequently or efficiently than the other side, can reflect the evolutionary history of species, their ecological characteristics, and cognitive abilities. Using anurans as a model system, it has been hypothesized that the strength of laterality (measured as the percentage of right-handed individuals) is higher in terrestrial and arboreal species, which exhibit asynchronous locomotion type (e.g., walking, climbing), than in aquatic species where synchronous locomotion type (e.g., swimming) predominates. We tested these predictions, including body size as an additional factor, in 19 Neotropical anuran species. For this, we obtained data on forelimb use preference for individuals of such species. We then combined these data with published data for another 14 anuran species obtained through a systematic review of the literature. The combined dataset was used to perform a phylogenetic comparative analysis to verify the strength of laterality in the species. We found that four out of the 19 tested species show a percentage of right-handed individuals that was higher or lower than expected by chance. Individuals of these four species exhibit asynchronous movements of forelimbs during displacements, foraging behaviors, sexual displays, or agonistic interactions. Results of a phyloANOVA and a phyloANCOVA indicated that the strength of laterality was higher in species with asynchronous movements, but this was unrelated to microhabitat and body size. Asynchronous (i.e., non-simultaneous) movements of limbs imply alternating muscle contractions and a higher neural asymmetry than synchronous (i.e., simultaneous) movements of limbs. Therefore, species with an asynchronous locomotion type could be reflecting such complexity and neural asymmetry in higher levels of forelimb laterality. Our results mostly support the predictions based on models that cover the expected relationship between the strength of laterality and the evolutionary ecology of vertebrates.

Cylindrospermopsin exposure promotes redox unbalance and tissue damage in the liver of Poecilia reticulata, a neotropical fish species

ABSTRACT There is a growing concern regarding the adverse risks exposure to cylindrospermopsin (CYN) might exert on animals and humans. However, data regarding the toxicity of this cyanotoxin to neotropical fish species are scarce. Using the fish species Poecilia reticulata, the influence of CYN concentrations equal to and above the tolerable for drinking water may produce on liver was determined by assessing biomarkers of antioxidant defense mechanisms and correlated to qualitative and semiquantitative histopathological observations. Adult females were exposed to 0.0 (Control); 0.5, 1 and 1.5 μg/L pure CYN for 24 or 96 hr, in triplicate. Subsequently the livers were extracted for biochemical assays and histopathological evaluation. Catalase (CAT) activity was significantly increased only by 1.5 μg/L CYN-treatment, at both exposure times. Glutathione -S-transferase (GST) activity presented a biphasic response for both exposure times. It was markedly decreased after exposure by 0.5 μg/L CYN treatment but significantly elevated by 1.5 μg/L CYN treatment. All CYN treatments produced histopathological alterations, as evidenced by hepatocyte cords degeneration, steatosis, inflammatory infiltration, melanomacrophage centers, vessel congestion, and areas with necrosis. Further, an IORG >35 was achieved for all treatments, indicative of the presence of severe histological alterations in P. reticulata hepatic parenchyma and stroma. Taken together, data demonstrated evidence that CYN-induced hepatotoxicity in P. reticulata appears to be associated with an imbalance of antioxidant defense mechanisms accompanied by histopathological liver alterations. It is worthy to note that exposure to low environmentally-relevant CYN concentrations might constitute a significant risk to health of aquatic organisms.

Effects of Deforestation on Foraging Behavior, Ectoparasites, and Adult Survival in the Vulnerable La Selle Thrush, Turdus swalesi, in Haiti

Although range-restricted Neotropical bird species are highly endangered, little is known about their ability to cope with environmental disturbance. We studied the vulnerable, Hispaniola- endemic La Selle Thrush (LST), Turdus swalesi, in a protected forested area in Haiti threatened by agriculture, livestock and logging. We used capture–mark–recapture, visual observations, and camera traps to document foraging ecology and estimate adult survival over 26 months. LST foraged mainly in deciduous woodlots. However, some individuals regularly foraged on or around dung pats in fallow pastures, whereas no other ground-dwelling bird species was observed to do so. Coincidently, 16.5% of 79 mist-netted LST harbored Ixodid ticks, compared to none of the 2131 individuals belonging to 29 other species mist-netted in the area. This suggests that infestation with ticks might come as a cost of opportunistically exploiting a new food resource. Apparent annual adult survival rate was independent of sex, and varied between 0.393 and 0.440, depending on the inclusion of a transience effect in our models. This low value was possibly due to “permanent” emigration from the site during the study. We discuss the potential effects of deforestation on the ecology and demography of LST and make recommendations for future conservation-oriented research in Haiti.

The relationship between wing morphology and foraging guilds: exploring the evolution of wing ecomorphs in bats

Abstract Different aspects of foraging strategies in bats have been studied to understand the evolution of flight in mammals. General descriptors of wing morphology associated with flight performance, such as aspect ratio and wing loading, allowed us to describe ecomorphs determined by the dietary preferences of bat species. However, the role of wing shape divergence in the evolution of bat foraging strategies remains little explored. We adopted a two-dimensional geometric morphometric approach to quantify the wing shape and size variation explained by phylogenetic relatedness (families) and to evaluate the covariance between foraging guilds and flight descriptors based on phylogenetic comparative methods in 69 Neotropical bat species. We tested whether wing morphology represents a reliable marker of the foraging guild, and we explored the rate of shape evolution among foraging guilds to describe divergent trends that could explain the morphological and ecological diversification. Our results suggest that the earliest bat ancestor was an aerial forager occupying the edge space, which is congruent with the observed evolution of wing shape from an edge space wing morphology. The relationship between wing shape and foraging space defines wing ecomorphs, which probably evolved early in bat ancestors; a process other than convergence could explain this association.

Redescription and neotype designation of Suphis fluviatilis Guignot, 1948 (Coleoptera: Dytiscoidea: Noteridae) and notes on other species of the genus.

Suphis fluviatilis Guignot, 1948 was described based on a single female specimen, and according to the author, the type was deposited in the Museum National dHistoire Naturelle (Paris, France). However, the diagnostic features of the specimen deposited under this name and provided with the respective labels deviate considerably from those given by Guignot in his original description. It is shown that this specimen belongs to Suphis notaticollis Zimmermann, 1921, confirming previous observations by Luis Grosso in 1980. Thus, a neotype of S. fluviatilis is designated to avoid instability of the nomenclature by the impending synonymy of S. fluviatilis with S. notaticollis. Suphis fluviatilis is redescribed in detail, including illustrations of the main diagnostic characters. The species is compared with other Neotropical Suphis species, in particular with S. notaticollis. Additionally, the male genitalia of Suphis intermedius Rgimbart, 1903 are described for the first time, and a first record from Paraguay is given.