Neonatal Body Weight Research Articles

성장에 영향을 주는 유전적.환경적 요인 분석에 대한 예비 연구

Objectives: Purpose of this study is to analyze and to estimate which and how much genetic and environmental factors have affected on growth. Also, a method of final height prediction can be developed from this study results. Methods: Correlation analysis and categorical regression analysis were conducted between genetic and environmental factors correlated with the final adult height, through survey from 171 male. Results: Mid parental height, neonatal body weight, intake frequency of beef, chicken, milk, fruits and coffee, sleep quantity and quality during the elementary school and sleep quantity during the middle school have affected on the final adult height. And a regression equation with 0.494 for coefficient of determination was obtained. Conclusions: Mid-parental-height has the most affected on the final adult height. Among environmental factors, food and sleep have significantly affected, but exercise doesn`t. Among foods, meal, beef, and milk intake have remarkably affected on the final height, and chicken and fruit also have affected in some degree, but coffee has affected badly. Among sleep habits, sleep quantity during the elementary school has the most affected, sleep quality during the elementary school and sleep quantity during the middle school also have affected in some degree on final height. The younger the age is, the more sleep have affected and sleep quantity have more affected than sleep quality. Neonatal weight also has remarkably affected on the final height. Through this analysis, the final adult height can be predicted using regression equation which covers 49.4% of genetic and environmental factors.

Outcome in Newborns from Pregnancies after in Vitro Fertilization

Background: Artificial reproductive technique - in vitro fertilization (IVF) has taken an important place in medicine as a routine and efficient method for the treatment of fertility. Aim: To examine outcome in newborns from pregnancies after IVF. Methods: A retrospective study included 260 newborns from pregnancies after IVF, which were divided into two groups. The first group consisted of 136 neonates from monofoetal IVF pregnancies and the second group consisted of 124 neonates from multifoetal IVF pregnancies. In newborns were analyzed: gender, gestation, anthropometric measures, Apgar score, morbidity and outcome. Results: The study found no difference in sex distribution between both groups of newborns from IVF pregnancies (p>0.05). Analysis found a significantly higher gestational age (37.6gw vs. 35.0gw, p< 0.01), lower rate of prematurity (20.5% vs. 64.5%, p< 0.01), greater birth body weight (3098.5g vs. 2238.7g, p< 0.01) and body length (49.9cm vs. 45.5cm, p< 0.01) in neonates from monofoetal IVF pregnancies. Analysis found significantly higher values of Apgar score at the first (8.5 vs. 7.0, p< 0.01) and at the fifth minute (9.3 vs. 7.8, p< 0.01) of birth in newborns from monofoetal IVF pregnancies. Testing has found a significantly lower morbidity in the first group of neonates (40.4% vs. 71.8%, p< 0.01), respectively, higher number of newborns from monofoetal IVF pregnancies had a favorable outcome (89.7% vs. 59.6%, p< 0.01). Conclusion: Newborns from monofoetal IVF pregnancies have a lower potential risk of perinatal complications with lower morbidity and better outcome, unlike newborns from multifoetal IVF pregnancies.

Role of iodine in diiodomethyl-p-tolylsulfone induced reproductive toxicity in rats: Proposed mode of action

The biocide diiodomethyl-p-tolylsulfone (DIMPTS) caused dystocia, decreased neonatal survival and hypothyroidism in rat reproduction studies resembling the effects caused by iodine. One molecule of DIMPTS contains two iodine moieties that are hydrolyzed upon ingestion and systemically absorbed, suggesting iodine toxicity as a probable mode of action for the effects observed in rats. This study compared the effects induced by DIMPTS and an equimolar concentration of its de-iodinated analogue, methyl-p-tolylsulfone (MPTS). Groups of 20 female Sprague Dawley rats were fed diets supplying 80mg DIMPTS/kg/day, 32mg MPTS/kg/day or control feed from prior to breeding through lactation and gonadal function, mating performance, conception, gestation, parturition, lactation, survival, growth and development of pups evaluated through postnatal day 7. Serum thyroid hormones and iodine levels in milk and sera were also determined. Females given DIMPTS had increased incidence of vulvar discharge and dystocia, decreased litter size, decreased body weights and feed consumption, increased thyroid weights, thyroid follicular cell hypertrophy with decreased colloid, decreased triidothyronine, and increased thyroid stimulating hormone levels. DIMPTS pups had decreased neonatal survival and body weights. These effects were associated with elevated levels of iodine in milk and sera. In contrast, MPTS did not produce similar effects in adult females or their offspring. These data support the hypothesis that the dystocia, altered neonatal survival and hypothyroidism following repeated dietary administration of DIMPTS were due to excessive iodine released from DIMPTS during absorption and metabolism.

Risk factors for postpartum hemorrhage in a cohort of 6011 Italian women

IntroductionPostpartum hemorrhage is responsible for 25% of maternal pregnancy-related deaths and it is the first cause of maternal morbidity and mortality worldwide. ObjectiveTo define the prevalence of postpartum hemorrhage and associated risk factors after vaginal birth and to develop a risk model that improves postpartum hemorrhage prediction. Patients and methodsAll women who underwent a vaginal delivery at the Obstetric Unit of a large University hospital in Milan (Italy) between July 2007and September 2009 were enrolled. Postpartum hemorrhage was defined as ≥500mL blood loss. A nomogram tailored to predict postpartum hemorrhage was developed, summarizing the impact of each covariate on the probability of postpartum hemorrhage. Results6011 women were studied (24% had blood loss ≥500mL and 4.8% ≥1000mL). Nulliparity, episiotomy, retained placenta and high neonatal body weight were confirmed as risk factors for postpartum hemorrhage. The odds ratio of postpartum hemorrhage was 0.86 (95%CI 0.78-0.90) for each 1gr/dL increase in ante-partum hemoglobin. An extensive internal validation of the developed nomogram demonstrated a good stability of the risk model. ConclusionsLow ante-partum hemoglobin is a new potentially modifiable risk factor for postpartum hemorrhage. A nomogram to predict the probability of postpartum hemorrhage is now available for external validation.

Body size of newborns in relation to mother's ethnicity and education: A pilot study from Vilnius city (Lithuania), 2005-2010

The purpose of this study was to analyse body size indices of newborns in Vilnius city (Lithuania) during 2005-2010 in relation with mother's education and ethnicity, and in parallel with the changes of socio-economic situation during the recent years. The present results were based on data (N = 18,084) from the Vilnius University Clinic of Obstetrics and Gynaecology. Comparison of the present Lithuanian data with other newborn studies was made. The analysis of socio-economic and demographic indicators of Lithuania, and the comparison of Gross Domestic Product of various countries was performed. The comparison of body size of newborns' data from the different countries showed that Lithuanian newborns were among the biggest babies. Some statistically significant differences in body size of newborns from different ethnic groups were established. Body length of Lithuanian newborns (M = 52.6 cm, SD = 2.5) was higher than length of Russian, Ukrainian and Romanian newborns. Body weight of Lithuanian newborns (M = 3511 g, SD = 485) was bigger than birth weight of Russian, Polish, Ukrainian and Romanian newborns. The analysis of newborns size by mother's education showed that body weight of neonates from mothers with the university education and from each other education group was bigger in comparison with the babies from respectively lower education group. The comparison of newborns weight by mother's ethnicity in relation to education level revealed nearly no discrepancies between size of newborns from mothers with the same education level at different ethnic group. The analysis of birth parameters by year has not established a statistically significant difference between the mean values for the body weight and body length of the whole investigated contingent of the full-term, single-birth newborns from Vilnius city during the 2005-2010. However, the tendency has been revealed that newborns from mothers with lower education were the most susceptible to negative economic changes after the 2008.

Effects of Ghrelin on the perinatal neonates

Ghrelin is a kind of brain-gut peptides,which integrates the body's digestive function and energy metabolism in the brain-gut axis.Recent studies showed that Ghrelin can be detected in breast milk.It is increasingly becoming the focus that Ghrelin from both maternal and perinatal neonates has special biological effects on the growth and development of newborns.such as stimulating the growth of fetal skin cells and the formation of bone,helping the catch-up growth of intrauterine growth restriction fetus after birth, regulating the glucose metabolism and promoting the fat synthesis of premature infants,and improving the maturation of neonatal immune system.This paper reviews the structure and physiological function of Ghrelin.as well as its effects on the neonatal growth and body weight regulation. Key words: Ghrelin; Fetus; Neonates; Growth and development

Acceptance of two liquid vitamin D3 formulations among mothers with newborn infants: a randomized, single-blind trial

In Switzerland, children are prescribed 7.5-12.5 μg per day of vitamin D(3) dissolved in alcohol, but many families do not adhere to the recommendation. The aim of the trial was to compare the acceptance of vitamin D(3) dissolved in alcohol or in medium-chain triglycerides among mothers of Swiss newborn infants. The acceptance was tested in 42 healthy newborn infants (20 girls and 22 boys) aged between 2 and 7 days. Their neonatal body weight ranged between 2.225 and 4.150 kg, and the gestational age between 36 1/7 and 41 3/7 weeks. The blinded mothers rated the facial reaction of their children by pointing on a facial hedonic scale. Thirty eight of the 41 mothers, who brought the comparison to completion, assigned a better score to the oily preparation with no difference in the remaining three cases (P < 0.0001). The acceptance for the oily preparation was significantly better both among mothers whose babies were initially presented the alcoholic preparation and among mothers whose babies were initially presented the oily preparation. Furthermore, the acceptance for the oily preparation was better irrespective of gender of the infant or parity of the mother. In conclusion, from the perspective of mothers, Swiss newborn infants prefer the taste of the oily vitamin D(3) preparation over the alcoholic preparation.

Perinatal supplementation with thymoquinone improves diabetic complications and T cell immune responses in rat offspring

BackgroundEpidemiological studies have shown that the offspring of mothers who experience diabetes mellitus during pregnancy are seven times more likely to develop health complications later in life compared to offspring born to nondiabetic mothers. Aim of the studyWe investigated whether supplementation with a natural antioxidant (thymoquinone; TQ) in female rats with streptozotocin (STZ)-induced gestational diabetes (GD) improved diabetic complications and T cell immune responses in their offspring. MethodsThree groups of female rats were tested: nondiabetics, diabetics treated with TQ during pregnancy and lactation periods and diabetics that were not treated with TQ (n=10 female rats in each group). ResultsOur data demonstrated a significant decrease in the numbers of neonates born to diabetic rats compared with those born to control rats. GD led to macrosomic pups with several postpartum complications, such as a significant increase in plasma levels of the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α (but not of IL-10); a marked decrease in the plasma level of IL-2; a marked reduction in the proliferative capacity of superantigen (SEB)-stimulated T-lymphocytes; and an obvious reduction in the number of circulating and thymus homing T cells. TQ supplementation of diabetic mothers during pregnancy and lactation periods had an obvious and significant effect on the number and mean body weight of neonates. Furthermore, TQ significantly restored the IL-2 level and T cell proliferation and subsequently rescued both circulating and thymus homing T cells in the offspring. ConclusionsOur data suggest that nutritional supplementation of GD mothers with the natural antioxidant TQ during pregnancy and lactation periods improves diabetic complications and maintains an efficient T cell immune response in their offspring, providing a protective effect in later life.

Relationships of Umbilical and Maternal Adiponectin, Resistin and Osteoprotegerin to Maternaland Newborn Anthropometric Characteristics

Introduction. Adiponectin, resistin and osteoprotegerin (OPG) are cytokines expressed in the adipose tissue. Pregnancy is associated with gradually increased maternal levels of these molecules, also detected in significant amounts in umbilical cord blood serum samples. Aim, patients and methods. To establish the relationships of maternal and umbilical adiponectin, resistin and OPG levels to both maternal and fetal anthropometric measurements and insulin sensitivity, 28 mother-newborn pairs were enrolled in the study. Blood samples were collected in a fasting state, after delivery, and serum insulin, C-peptide, sex hormone-binding globulin, adipocytokines, OPG and bone specific alkaline phosphatase (BAP) were measured. Results. Compared to maternal values, umbilical serum adiponectin levels were about 3-fold higher; additionally, significantly higher resistin and lower OPG levels were observed. Stratification of umbilical and maternal adiponectin levels according to tertiles of birth body weight demonstrated significantly lower maternal adiponectin levels by tertiles of neonatal body weight. No relationships were noticed between infant birth weight and maternal or umbilical serum resistin and OPG, respectively. Umbilical resistin was significantly associated to both maternal resistin and umbilical adiponectin. Multiple regression analysis showed that maternal BMI, umbilical insulin, C-peptide and resistin explained 71.83% of umbilical serum adiponectin variability. Umbilical resistin was independently predicted by umbilical adiponectin, umbilical C-peptide and maternal BMI, and the model explained 81.49% of umbilical resistin levels. Conclusions. In human, umbilical serum adiponectin and resistin levels are significantly higher compared to adults. These adipokines may mediate the effects of maternal body mass on fetal development. The biology of the OPG/RANKL cytokine system in fetuses and newborns needs further research.

Maternal lipid metabolism during normal pregnancy and its implications to fetal development

Whereas accumulation of fat depots occurs during the first two-thirds of gestation, increased adipose tissue lipolysis and hyperlipidemia develops in the last trimester. Insulin resistance and increased estrogens in late pregnancy contribute to these changes. Lipoprotein receptors and fatty acid binding proteins in the placenta allow the transfer of long-chain polyunsaturated fatty acids to the fetus. Enhanced oxidative stress in pregnancy may be related to maternal hyperlipidemia. Maternal plasma nonesterified fatty acids and cholesterol correlate with those in the fetus, and maternal adipocytokines have been associated with fetal growth. However, positive correlations between maternal nonesterified fatty acids or triacylglycerols and neonatal body weight or fat mass have only been found in gestational diabetics. Under the limited capacity of the fetus to modify the structure of essential polyunsaturated fatty acids, their long-chain derivatives have to be transferred from the mother through the placenta in appropriate amounts to warrant the normal fetal development.

The effects of pre-pregnancy body mass index and gestational weight gain on neonatal birth weight in Taiwan

The aim of this retrospective study was to explore the effects of pre-pregnancy body mass index and gestational weight gain on neonatal birth weight in Taiwan.Study subjects included two hundred and sixty three women who delivered their babies at two local hospitals in southern Taiwan. Initial data included maternity records on age, parity, socio-economic status, body mass index (BMI), pregnancy weight gain, neonatal gender, and neonatal birth weight. The mean age of 263 women was 29.99 years (range 19 - 42). The mean BMI was 21.19 kg/m2 (range 16.22 - 32.05), and the mean pregnancy weight gain was 13.98 kg (range 3 - 33.5). The mean neonatal birth weight was 3192.57 gm (range 2120 - 4390). Mothers whose BMI was 24 - 27 had significantly higher neonatal birth weight than those mothers with a BMI > 27 and < 18.5) (F = 5.816, p = 0.001). After dividing the weight gain during pregnancy into four groups based on the recommendation of the Department of Health in Taiwan, our results show differences between maternal weight gain and neonatal body weight (F = 9.49, p < 0.001). Pregnant women with a weight gain less than 10 kg resulted in deliveries of neonates with lower birth weight (113.94, 237.62 and 332.58 gm) than those mothers who gained 10 - 14, 14 – 16 kg, and more than 16kg. Multiple regression models control for other maternal and neonatal characteristics were able to document, weight gain as being more strongly associated with neonatal birth weight than pre-pregnancy body mass index. Neonatal birth weight was not correlated with maternal working status, socio-economical status and was not differentiated between primipara and multipara status. Conclusively, pre-pregnancy BMI and prenatal weight gain are related to neonatal birth weight. These results suggest that the pre-pregnancy BMI and prenatal weight gain should be among the most important concerns for providers offering prenatal services. Hopefully these findings will serve as a useful reference for prenatal nurses and will reinforce health insurance departments’ efforts to ensure quality prenatal care. Key words: Body mass index, pregnancy weight gain, neonatal birth weight

Effects of Sanyinjiao (SP6) with electroacupuncture on labour pain in women during labour

To evaluate the effects of Sanyinjiao (SP6) with electroacupuncture on labour pain in women during the labour process. A total of 350 women in labour from three centres were randomly divided into the electroacupuncture group (acupuncture group), the sham electroacupuncture group (sham group) and the control group. Women in labour in the electroacupuncture group received the electroacupuncture on the point Sanyinjiao (SP6). The analgesic effect was self-rated by women in labour, using visual analogue scale (VAS). The duration and paralysis time of uterine contraction, uterine contraction regularity, degree of cervical extension, presentation of foetal descent, the condition of intra-partum haemorrhage and postpartum haemorrhage, labour manner, lochia, involution of uterus, milk secretion, neonate Apgar Score and neonate body height and weight were also measured. All data were analysed with Statistical Package for Social Sciences (SPSS) 12.0. Labour pain scores from women in the acupuncture group were less than in the control group at needle retaining at 30min, 2 and 4h after needle withdrawal. Other evaluated indices did not show significant differences among the three groups. No adverse events were observed during the labour process. SP6 with electroacupuncture could be an effective way for decreasing labour pain.

Effects of in ovo feeding of carbohydrates and arginine on hatchability, body weight, energy metabolism and perinatal growth in duck embryos and neonates

1. The objective of this study was to test the hypothesis that in ovo feeding of carbohydrates and arginine into the duck amnion may improve the glycogen store and perinatal growth. At 23 d of incubation, fertile eggs were injected with 1·2 ml of sodium chloride (NaCl), sucrose + maltose (CHO), arginine (Arg) or sucrose + maltose + arginine (CHO + Arg), with controls not injected. Body weight, liver and muscle glycogen levels, and hepatic glucose-6-phosphatase activity were determined at 25 d of incubation, at hatch, and at 3 and 7 d posthatch. 2. At hatch and 7 d of age, the body weights were greater in the in ovo-feeding treatments than the controls. Arg and CHO + Arg significantly enhanced liver glycogen level at hatch compared with controls. CHO and CHO + Arg significantly increased muscle glycogen level at 25 d of incubation over controls. CHO and Arg decreased glucose-6-phosphatase at 25 d of incubation, whereas NaCl and CHO + Arg increased glucose-6-phosphatase at hatch relative to controls. 3. In ovo feeding of carbohydrates and arginine at 23 d of incubation may improve glycogen reserves, which may, in turn, provide the energy needed for perinatal growth.

The Application of a Bi-ventricular Assist Device for a Low Weight (2.4 kg) Neonate with Coarctation of the Aorta and Critical Aortic Stenosis

A 5-day-old neonate (body weight=2.4 ㎏) with coarctation of the aorta and critical aortic stenosis underwent an interventional balloon valvuloplasty for aortic stenosis. During the intervention, cardiac arrest occurred due to injury of the right carotid artery by the guide wire. An extracorporeal membrane oxygenator (ECMO) was applied. After 1 day’s support, total surgical correction was achieved; however, in the immediate postoperative period, cardiac function was severely depressed. We applied a bi-ventricular assist device (bi-VAD) instead of an ECMO and we were able to wean the patient off the bi-VAD device after 3 days’ support. The patient was discharged without severe complications.

Effects of Different Oil Sources and Vitamin E in Breeder Diet on Egg Quality, Hatchability and Development of the Neonatal Offspring

Effects of polyunsaturated fatty acid (PUFA) and vitamin E (VE) supplementation in the diet of breeder hens on the egg quality and hatchability, lipid peroxides of the egg yolk, and development of the newly-hatched offspring chicks were investigated. A total of 800 Avian 48, 28 wk-old broiler breeders were assigned randomly to 4 groups with 4 replicates of 45 females and 5 males. Each group was fed one of the following four diets with different oil sources and levels of VE: corn oil (CO), fish oil (FO), CO+VE and FO+VE. The results showed that: i) Addition of FO in the breeder diet reduced the whole egg weight, yolk weight, albumen weight, yolk color score and neonatal offspring chick body weight without affecting the hatchability as compared to the CO treatment. ii) Addition of VE efficiently reduced the lipid peroxides of egg yolk from hens fed diets containing FO. iii) VE in the breeder diet significantly promoted the development of liver and heart of the chick offspring.

OP23.02: Amniotic fluid erythropoietin like matter of distinction between IUGR and constitutionally small fetuses

To determine the possibilities of analysis of erythropoietin (EPO) in amniotic fluid as a parameter independent of gestational age and subjective interpretations upon making a distinction between IUGR and constitutionally small fetuses. Prospective clinical study was conducted on 38 pregnancies with fetuses small for gestational age. Small for gestational age fetuses (SGA) have been defined as both estimated fetal weight and neonatal body weight beyond 10th percentile for our population. The study group was divided into two subgroups: constitutionally small fetuses, n = 16 (42.11%) and growth restricted fetuses, n = 22 (57.89%). The diagnosis of both, constitutionally small and growth restricted fetuses, were based upon standard criteria (fetal biometry as well as AFI and Doppler evaluation). Amniotic fluid samples were collected in all pregnancies in order to evaluate EPO levels, by amniocenthesis for determination of lung maturity, maximum 48 hours before delivery EPO concentration was higher in growth restricted fetuses compared to constitutionally small fetuses showing high statistical significance (p < 0.01). ROC analysis for EPO values comparing growth restricted fetuses and constitutionally small fetuses has the best result at the level of 9.8 IU/L. This cut-off point has sensitivity of 81%, specificity of 80%, PPV 81.2% and NPV 88.4%. Neonates with EPO concentrations above 9.8 IU/l were more frequent admitted to the NICU and concentrations of EPO showed statistical significance (p < 0.05) comparing to those without admittance at NICU. There is a difference in amniotic fluid EPO concentrations between growth restricted and constitutionally small fetuses. Concerning the fact that EPO production is independent of gestational age, EPO concentrations may be used as a distinction between the pathologically and physiologically small for gestational age fetuses when amniocentesis is done for the detection of fetal lung maturity or rapid karyotypisation

The relationship between breast milk leptin and neonatal weight gain

To investigate whether change in leptin content of breast milk during lactation acts on neonatal body weight gain. In total 15 lactating women and their 15 term infants were involved in the study. Breast milk and neonatal serum samples were obtained from the same women and their neonates on the 1st day and any day between the 21st and 30th days after birth. Breast milk and serum leptin concentrations were determined by radioimmunoassay. Anthropometric indexes of the infants were recorded. The study was completed with 15 multiparious mothers aged 19-37 years and their infants. The mean collection time of the first samples after birth was 6.07 +/- 1.94 h. The leptin level in the mature milk was significantly higher than in the colostrum (p < 0.001). Neonatal weight and height were significantly increased on 21-30 lactation days compared to 1st day of lactation (p < 0.05 and p < 0.001, respectively). The leptin concentration in the mature milk was negatively correlated with delta BMI (r =-0.53; p < 0.05). The delta breast milk leptin concentration was also found to be inversely correlated with delta BMI (r =-0.529; p < 0.05). The results of this study have suggested that change in the leptin content of breast milk during lactation might play a role in the regulation of weight gain in healthy neonates.

Effect of lactational exposure of olanzapine on body weight of mice: a comparative study on neonates of both the sexes during post-natal development

Adverse impact of atypical antipsychotic drugs on body weight of adult and juvenile groups has been well-documented both at clinical and preclinical investigations. However, studies on impact of drug on body weight during fetal or neonatal development received little attention. The present study is the first-ever preclinical investigation demonstrating the effect of lactational exposure of olanzapine (4, 8, and 10 mg/kg) and risperidone (1 and 2 mg/kg), two widely prescribed antipsychotics, on body weight of mice neonates. Body weight gain was observed with both the drugs, although a sex-related differential response was noted. In olanzapine-exposed female neonates, the weight gain was more with the highest dose. Male neonates showed a reverse trend, i.e. the highest gain with the lowest dose. Female neonates exposed to risperidone also showed significant, but less gain as compared to their olanzapine-exposed counterparts. Risperidone-exposed male neonates showed little body weight gain. Waist-to-hip ratio and plasma prolactin level were measured to explain the reason behind the body weight gain, but there were deviations with respect to drug and sex. The body weight gain may be the overall manifestations of drug-induced endocrine and metabolic dysregulations.

Effects of early nursing intervention on neonatal jaundice and weight

Objective To study the effects of early intervention on neonatal jaundice and body weight, thereby preventing the occurrence of hyperbilirubinemia in newborns. Methods 320 healthy newborns were randomly divided into the two groups of the intervention group and the control group, with 160 cases in each group. All neonates of the intervention group were given the early intervention, including early breast-feeding, formula milk before the adequate breast-feeding, swimming and massaging. There was no nursing intervention for the control group, only the general nursing. The first defecation time, the first yellow defecation time, body weight in everyday, bilirubin value per cutem were measured and recorded. Results The bilirnbin value of the intervention group was much lower than that of the control group. The difference had statistical meaning (P < 0. 01). The body weight of newborns in the intervention group was much higher than that of the control group 5 days after born. There was statistical difference between the two groups (P < 0. 01). Conclusions Early nursing intervention can mitigate the bilirubin value effectively, reduce the incidence rate of hyperbilirnbinemia in newborns, reduce the decrease of body weight in newborns, and raise the body weight on discharge. Key words: Neonatal jaundice; Body weight; Nursing intervention

Analysis of PFOA in dosed CD1 mice: Part 1. Methods development for the analysis of tissues and fluids from pregnant and lactating mice and their pups

The number of studies involving the analysis of perfluorooctanoic acid (PFOA) has increased recently because PFOA is routinely detected in human blood samples from around the world. Recent studies with mice have shown that dosing pregnant dams with PFOA during gestation gives rise to a dose-dependent mortality in the litters, a reduction in neonatal body weight for the surviving pups, and subsequent deficits in mammary gland development when compared to control animals. The actual body burdens of PFOA in dams and pups associated with these endpoints have not been determined, in part due to a lack of robust analytical methods for these matrices. The goal of the current study was to develop reliable methods with acceptable performance characteristics for the analysis of PFOA in several matrices relevant to pregnant mouse studies. Dam and pup serum, amniotic fluid, urine, milk, mammary tissue, and whole mouse pups were isolated for method development and analysis. The resulting method provided excellent accuracy (92.1–111%) and reproducibility (relative standard deviation 4.3–21%) making them very useful for future studies. These methods were then applied to dosed animal fluids and tissues in order to conduct a thorough evaluation of the pharmacokinetics in utero. Resulting tissue specific measurements of PFOA in serum, amniotic fluid, urine, milk, mammary tissue, and whole pup homogenate will be used to more completely describe the dose–response relationships for the most sensitive health outcomes and inform pharmacokinetic models that are being developed and evaluated.