Neolithic Dog Research Articles

Eurasian Grey and White wolf ancestors—800,000 years evolution, adaptation, pathologies and European dog origins

AbstractThe oldest known wolf appears 800,000 years ago (Marine Isotope Stage 21) in Eurasia with the unspecialized short‐legged old Mammoth steppe wolf Canis lupus bohemica nov. spec. From this species, about 600,000–420,000 years ago (MIS 15‐11), the interglacial Canis lupus mosbachensis (Soergel, 1925) short‐legged Mosbach grey wolf subspecies roamed Eurasia. In the late Middle Pleistocene, there are two lineages, the southern interglacial grey and northern glacial White wolves in Eurasia. Since 320,000 (MIS 8), the short‐legged White wolf Canis lupus spelaeus (Goldfuss, 1823) was the glacial Mammoth steppe‐adapted wolf. Parallel to the “cave wolf” (found in the German Zoolithen Cave), the warm climate grey wolf Canis lupus brevis Kuzmina and Sablin, 1994 existed. C. l. spelaeus relates to the Holocene (MIS 1) extant Holarctic Greenland Canis lupus arctos and Siberian Canis lupus albus (Kerr, 1792). The Late Palaeolithic (MIS 2) “Gravettian Goyet dogs” fall into the DNA pool of C. l. spelaeus and are identified herein as pathological bite trauma individuals, which braincase shortened during the healing process. European prehistoric Neolithic dogs seem to have been imported from Central Asia with the Bandkeramik people (approx. 7000 BP) first, which have the stepped frontals originating from grey wolves.

The gut microbiome buffers dietary adaptation in Bronze Age domesticated dogs

SummaryIn an attempt to explore the role of the gut microbiome during recent canine evolutionary history, we sequenced the metagenome of 13 canine coprolites dated ca. 3,600–3,450 years ago from the Bronze Age archaeological site of Solarolo (Italy), which housed a complex farming community. The microbiome structure of Solarolo dogs revealed continuity with that of modern dogs, but it also shared some features with the wild wolf microbiome, as a kind of transitional state between them. The dietary niche, as also inferred from the microbiome composition, was omnivorous, with evidence of consumption of starchy agricultural foods. Of interest, the Solarolo dog microbiome was particularly enriched in sequences encoding alpha-amylases and complemented a low copy number of the host amylase gene. These findings suggest that Neolithic dogs could have responded to the transition to a starch-rich diet by expanding microbial functionalities devoted to starch catabolism, thus compensating for delayed host response.

Bronze Age Coprolites Unveil the Buffering Role of the Gut Microbiota in the Dietary Adaptation of Dogs to Domestication

Dog evolution has long been debated, particularly with regards to the origin of domestication and its effects on the genome, but little is known about the role of the gut microbiome during the recent canine evolutionary history. We sequenced the metagenome of 13 canine coprolites dated ca. 3,600-3,450 y ago from the Bronze Age archaeological site of Solarolo (Italy), which housed a complex farming community. The microbiome structure of Solarolo dogs revealed continuity with that of modern dogs, but it also shared some features with the wild wolf microbiome, as a kind of transitional state between them. The dietary niche, as also inferred from the microbiome composition, was omnivorous, with evidence of consumption of starchy agricultural foods. Interestingly, the microbiome from Solarolo dogs was particularly enriched in sequences encoding alpha-amylases and complemented a low copy number of the host amylase (AMY2B) gene. These findings suggest that Neolithic dogs could have responded to the transition to a starch-rich diet by expanding microbial functionalities devoted to starch catabolism. Such a microbiome adaptive mechanism would have compensated for delayed host response to the new primarily abundant plant food resources, available from companionship with Bronze Age agriculturalists.

Evidence for Early European Neolithic Dog Dispersal: New Data on Southeastern European Subfossil Dogs from the Prehistoric and Antiquity Ages.

The history of dog domestication is still under debate, but it is doubtless the process of an ancient partnership between dogs (Canis familiaris) and humans. Although data on ancient DNA for dog diversity are still incomplete, it is clear that several regional dog populations had formed in Eurasia up to the Holocene. During the Neolithic Revolution and the transition from hunter-gatherer to farmer societies, followed by civilization changes in the Antiquity period, the dog population structure also changed. This process was due to replacement with newly formed dog populations. In this study, we present for the first time mitochondrial data of ancient dog remains from the Early Neolithic (8000 years before present (BP)) to Late Antiquity (up to 3th century AD) from southeastern Europe (the Balkans). A total of 16 samples were analyzed, using the mitochondrial D-loop region (HVR1). The results show the presence of A (70%) and B (25%) clades throughout the Early and Late Neolithic Period. In order to clarify the position of our results within the ancient dog population in Eneolithic Eurasia, we performed phylogenetic analysis with the available genetic data sets. This data showed a similarity of the ancient Bulgarian dogs to Italian (A, B, and C clades) and Iberian (clades A and C) dogs’ populations. A clear border can be seen between southern European genetic dog structure, on the one hand, and on the other hand, central-western (clade C), eastern (clade D) and northern Europe (clades A and C). This corresponds to genetic data for European humans during the same period, without admixture between dog populations. Also, our data have shown the presence of clade B in ancient Eurasia. This is not unexpected, as the B haplogroup is widely distributed in extant Balkan dogs and wolves. The presence of this clade both in dogs and in wolves on the Balkans may be explained with hybridization events before the Neolithic period. The spreading of this clade across Europe, together with the A clade, is related to the possible dissemination of newly formed dog breeds from Ancient Greece, Thrace, and the Roman Empire.

Retrieving ascarid and taeniid eggs from the biological remains of a Neolithic dog from the late 9th millennium BC in Western Iran.

BACKGROUND Paleoparasitology reveals the status of parasitic infections in humans and animals in ancient times based on parasitic particles found in biological remains from archaeological excavations. This line of research emerged in Iran in 2013.OBJECTIVE The identification of parasites from Neolithic times is an attractive subject that shows the oldest origins of parasitic infections in a given geographical region. From an archaeological point of view, this archaeological site is well-known for animal domestication and agriculture in ancient Iran.METHODS In this study, soil deposited on the surface and in the pores of a dog pelvic bone was carefully collected and rehydrated using trisodium phosphate solution.FINDINGS The results showed ascarid and taeniid eggs retrieved from the biological remains of a dog excavated at the East Chia Sabz archaeological site, which dates back to the Neolithic period (8100 BC).MAIN CONCLUSION The current findings clearly illustrate the natural circulation of nematode and cestode parasites among dogs at that time. These ancient helminth eggs can also be used to track the oldest parasitic infections in the Iranian plateau and contribute to the paleoparasitological documentation of the Fertile Crescent.

Ancient European dog genomes reveal continuity since the Early Neolithic

Europe has played a major role in dog evolution, harbouring the oldest uncontested Palaeolithic remains and having been the centre of modern dog breed creation. Here we sequence the genomes of an Early and End Neolithic dog from Germany, including a sample associated with an early European farming community. Both dogs demonstrate continuity with each other and predominantly share ancestry with modern European dogs, contradicting a previously suggested Late Neolithic population replacement. We find no genetic evidence to support the recent hypothesis proposing dual origins of dog domestication. By calibrating the mutation rate using our oldest dog, we narrow the timing of dog domestication to 20,000–40,000 years ago. Interestingly, we do not observe the extreme copy number expansion of the AMY2B gene characteristic of modern dogs that has previously been proposed as an adaptation to a starch-rich diet driven by the widespread adoption of agriculture in the Neolithic.

Genomic and archaeological evidence suggest a dual origin of domestic dogs.

The geographic and temporal origins of dogs remain controversial. We generated genetic sequences from 59 ancient dogs and a complete (28x) genome of a late Neolithic dog (dated to ~4800 calendar years before the present) from Ireland. Our analyses revealed a deep split separating modern East Asian and Western Eurasian dogs. Surprisingly, the date of this divergence (~14,000 to 6400 years ago) occurs commensurate with, or several millennia after, the first appearance of dogs in Europe and East Asia. Additional analyses of ancient and modern mitochondrial DNA revealed a sharp discontinuity in haplotype frequencies in Europe. Combined, these results suggest that dogs may have been domesticated independently in Eastern and Western Eurasia from distinct wolf populations. East Eurasian dogs were then possibly transported to Europe with people, where they partially replaced European Paleolithic dogs.

Y Chromosome Analysis of Dingoes and Southeast Asian Village Dogs Suggests a Neolithic Continental Expansion from Southeast Asia Followed by Multiple Austronesian Dispersals

Dogs originated more than 14,000 BP, but the location(s) where they first arose is uncertain. The earliest archeological evidence of ancient dogs was discovered in Europe and the Middle East, some 5-7 millennia before that from Southeast Asia. However, mitochondrial DNA analyses suggest that most modern dogs derive from Southeast Asia, which has fueled the controversial hypothesis that dog domestication originated in this region despite the lack of supporting archeological evidence. We propose and investigate with Y chromosomes an alternative hypothesis for the proximate origins of dogs from Southeast Asia--a massive Neolithic expansion of dogs from this region that largely replaced more primitive dogs to the west and north. Previous attempts to test matrilineal findings with independent patrilineal markers have lacked the necessary genealogical resolution and mutation rate estimates. Here, we used Y chromosome genotypes, composed of 29 single-nucleotide polymorphism (SNPs) and 5 single tandem repeats (STRs), from 338 Australian dingoes, New Guinea singing dogs, and village dogs from Island Southeast Asia, along with modern European breed dogs, to estimate the evolutionary mutation rates of Y chromosome STRs based on calibration to the independently known age of the dingo population. Dingoes exhibited a unique haplogroup characterized by a single distinguishing SNP mutation and 14 STR haplotypes. The age of the European haplogroup was estimated to be only 1.7 times older than that of the dingo population, suggesting an origin during the Neolithic rather than the Paleolithic (as predicted by the Southeast Asian origins hypothesis). We hypothesize that isolation of Neolithic dogs from wolves in Southeast Asia was a key step accelerating their phenotypic transformation, enhancing their value in trade and as cargo, and enabling them to rapidly expand and replace more primitive dogs to the West. Our findings also suggest that dingoes could have arrived in Australia directly from Taiwan, independently of later dispersals of dogs through Thailand to Island Southeast Asia.

Canids as persons: Early Neolithic dog and wolf burials, Cis-Baikal, Siberia

Interpretations of dog burials made by ancient foraging groups have tended to be based upon our own relationships with such animals and modern western cosmological and ontological concepts. Osteological studies of early dogs often focus only on issues of taxonomy, and as a result very little is known about these animals’ life histories. Eastern Siberia has produced many Holocene dog burials, but these are typically not well described and the explanatory frameworks provided for them are very underdeveloped. Here we examine in detail two Cis-Baikal canid burials, one of a wolf and the other a dog, both in large Middle Holocene hunter-gatherer cemeteries. We link the mortuary treatment of these animals to other cultural practices, particularly the treatment of the human dead, and broader patterns in Northern human-animal relationships. This interpretive model is combined with detailed osteobiographies for the canids and contextual information for these and other dogs and wolves from Middle Holocene Cis-Baikal. It is argued that canids here were understood and treated in a variety of ways. We suggest that some animals with unique histories were known as distinct persons with ‘souls’ and because of this at death required mortuary rites similar to those of their human counterparts.

Ancient DNA supports lineage replacement in European dog gene pool: insight into Neolithic southeast France

We report palaeogenetic analysis of domesticated dog ( Canis familiaris) remains excavated from three archaeological sites from southeast France and dating from Middle Neolithic. Ancient DNA analysis was attempted on teeth and bone samples taken from 11 dogs. Three 266-base-pair fragments of the mitochondrial genome Hypervariable Region I (HVR-I) could be retrieved and revealed two haplotypes belonging to HVR-I lineage C. These three sequences were compared to the sequences of Swedish and Italian Neolithic dogs and permitted to confirm that clade C was largely represented all over Western Europe during this period. One haplotype defined in Neolithic French dog was observed for the first time in Canis mtDNA, underlining the loss of mitochondrial diversity in Europe since the Neolithic. Finally, these results point out mitochondrial lineage replacement in Europe, since lineage C represents only 5% of extant European dogs. Altogether, these results support the proposition that palaeogenetic studies are essential for the reconstruction of the past demographic history and the domestication process of dogs.

Two New Dogs, and Other Natufian Dogs, from the Southern Levant

Two new fairly complete remains of dogs were uncovered from a burial in Hayonim Terrace (northern Israel), dated to the first half of the 11th millenium (bp) (Late Natufian). This burial contained the remains of three humans associated with an elaborate construction. A detailed analysis of these dogs, and a comparison with all known Natufian remains, suggested that genuine dogs were already living around and within human habitations during this period. While most studies on early dogs were carried out on post-Natufian material, a period during which intentional selection could have already been widely experienced, we show that the evolvement of Natufian dogs were the product of unconscious selection of commensal wolves quasi-isolated under the special anthopogenic habitats created within and around Natufian sites, and at least ritually assimilated to human society. There is no evidence that Neolithic dogs are direct descendants of Natufian ancestors. Their multiregional origination is a widely accepted phenomenon. We suggest that Neolithic dogs were either domesticated anew, or were introduced from elsewhere to the southern Levant. Although the number of specimens of Natufian dogs is still small, evidently the emergence of some patterns clearly indicate that all them were already markedly different from the recent southern Levantine wolves and, in spite of their widely ranged morphology, constituted a unique group. It is shown that the shortening of the muzzle mainly affected the anterior part of the snout, while the posterior region remained practically unchanged. In this respect they seem to display a typical case of paedomorphosis. The simultaneous diminution of the carnassials, and other teeth, alongside with the snout, was marked enough that no crowding of the teeth took place by any of the Natufian dogs. The disproportional reduction of the snout versus teeth that caused the “crowding” phenomenon is only known from later periods.

Neolithic dogs: A reappraisal based on evidence from the remains of a large canid deposited in a ritual feature

A canid skull and mandible, dated to the late neolithic, produced a series of measurements which indicate an animal significantly larger than any dog recovered from this period. The skull showed evidence of healed trauma with associated asymmetry. Metrical and morphological criteria have been applied to identify the remains as those of wolf Canis lupus, and the results of these tests are inconclusive. The possibility that the remains are of a large dog, Canis familiaris, is discussed, together with the implications that this may have for the archaeological consideration of this species.

Neolithic dogs: A reappraisal based on evidence from the remains of a large canid deposited in a ritual feature

Neolithic dogs: A reappraisal based on evidence from the remains of a large canid deposited in a ritual feature

A further consideration of neolithic dogs with special reference to a skeleton from Grime's Graves (Norfolk), England

The complete skull and skeleton of a dog were recently excavated from the neolithic flint mines at Grime's Graves in Norfolk. As very few complete skeletons of dogs have been found from neolithic contexts in Britain a detailed metrical comparison was made between the Grime's Graves specimen and dog skeletons of comparable antiquity and completeness from Easton Down and Windmill Hill in Wiltshire. Implicit in the study of the dog from Grime's Graves was the need to date it precisely. As the skeleton could not be sacrificed for radiocarbon dating, closely associated antlers of Red deer ( Cervus elaphus) were used for this purpose. The date of the Grime's Graves dog is compared with the dates for other dog remains from recently excavated neolithic sites in the British Isles.