The advantages/limitations obtained by working in dynamic scanning forcemicroscopy (DSFM) at different levels of tip/sample interaction forces (from thenet attractive to the hard repulsive regime) are experimentally shown by imagingmonolayers containing novel fibre-like supramolecular nanostructures wrapped upin spiral-like domains. The structures have been obtained by using theLangmuir–Blodgett technique and transferring onto mica monolayers ofquercetin-3-O-palmitate molecules mixed with a fraction of about 25% ofdimyristoylphosphatidylcholine. The measurements in the attractive regimeallowed us to reveal morphological features of the supramolecular structures thatcannot be demonstrated by the conventional repulsive regime. In particular, byattractive dynamic scanning force microscopy (DSFMA) the height ofthe fibre-like structures was a factor of two to three higher and peculiarnano-ruptures along the wrapped fibres have been observed. The influence of thetip/sample contact interaction was investigated by recording images in hardtapping and successively imaging the same region in DSFMA as well as bycomparing the images in DSFMA with those obtained in negative lift modeforce modulation microscopy, phase imaging and friction force microscopy.