Needle Conductance Research Articles

Growing season ecophysiology ofTaxodium distichum(L.) Rich. (bald cypress) saplings in a restored wetland: a baseline for restoration practice

Wetland restoration is a common practice in the southeastern United States, where changing land use patterns have resulted in the widespread degradation of coastal ecosystems. Re-establishment of indigenous vegetation is a fundamental paradigm of restoration practice. In freshwater wetlands, this often includes planting flood-tolerant species such as Taxodium distichum (L.) Rich (bald cypress). While much is known regarding the ecology of this foundational species, research establishing the ecophysiological profile of mature bald cypress under stress-free, baseline conditions is limited. Therefore, a study was undertaken in 2015 to monitor the growing season ecophysiology of a bald cypress stand in a restored coastal wetland. Net photosynthesis was constant in the early to mid-growing season, declining steadily late in the growth season until the visible onset of needle senescence. Patterns in transpiration matched those observed for photosynthesis, whereas needle conductance and chlorophyll fluorescence appeared to track expected patterns associated with needle development. Trees at this site appeared healthy and were reproductively competent. However, long-term monitoring of the stand would help evaluate the ecological sustainability of this restoration project. We advocate for the inclusion of ecophysiological-based vegetation monitoring within restoration projects, enabling a sensitive and early indication of the trajectory of a restoration plan.

Structure, pigment content, and photosynthesis of Siberian larch needles in Northern and Sub-Arctic Urals

The structure and functional organization of the photosynthetic apparatus of Siberian larch growing on the western slope of Northern and Sub-Arctic Urals were studied. The needle morphostructure, the mesophyll cell ultrastructure, and the pigment complex of chloroplasts were quantitatively characterized. Active photosynthesis of larch was observed during warm days with a low deficit of humidity. Photoassimilation depression under hot and dry weather was followed by a decrease in the stomatal conductance of needles. Under extreme conditions of mountains, Siberian larch has low photosynthesis. It decreased gradually from Nothern to Sub-Arctic Urals.

Carbon Isotope Discrimination, Gas Exchange, and Growth of Container-grown Conifers Under Cyclic Irrigation

The objective of this study was to quantify the effects of cyclic irrigation on growth and physiology of container-grown conifer species in pot-in-pot (PIP) production in the upper Midwest. Trees of four conifer species (Picea glauca var. densata, Picea pungens, Abies fraseri, and Pinus strobus) were grown in 25-L containers and assigned to one of four combinations of irrigation rate (low or high) and daily irrigation cycle frequency (one or four). Irrigation rates were based on common nursery practice in the region (2 cm rainfall equivalent/day) and one-half the standard rate (1 cm rainfall equivalent/day). Cyclic irrigation increased relative height growth and relative caliper growth of Pinus strobus by over 80% and 35%, respectively, compared with once-daily irrigation. The high-rate irrigation increased relative caliper growth of Picea pungens by 40% compared with the low rate. The effects of irrigation regime on needle- or shoot-level gas exchange varied by species and date of measurement. Carbon isotope discrimination (Δ13C) of needle and wood tissue was positively correlated (r ≥ 0.64, P < 0.001) with needle conductance to water vapor (gwv) and negatively correlated (r ≤ −0.60, P< 0.001) with intrinsic water use efficiency (WUEi). Carbon isotope discrimination of wood and needle tissue decreased with the low irrigation rate, indicating increased WUEi associated with reduced gwv. Cyclic irrigation had relatively little effect on Δ13C except for Pinus strobus. Our findings suggest that carbon isotope composition of wood and needle tissue provides a sensitive and accurate representation of plant response to varying moisture availability. From a water management perspective, identifying optimal irrigation rates appears to be more important than number of daily cycles for these crops grown in the midwestern United States.

Large probe arrays for measuring mean and time dependent local oil volume fraction and local oil velocity component distributions in inclined oil-in-water flows

Arrays of dual-sensor and four-sensor needle conductance probes have been used to measure the mean and time dependent local properties of upward inclined, bubbly oil-in-water flows (also known as dispersed oil-in-water flows) in a 153mm diameter pipe. The flow properties that were measured were (i) the local in-situ oil volume fraction α; (ii) the local oil velocity uo in the axial direction of the pipe (the Z direction); and (iii) the local oil velocity uY in the direction from the lower side of the inclined pipe to its upper side (the Y direction). Oil velocities in the X direction (orthogonal to the Y and Z directions) were found to be negligible. For all of the flow conditions investigated it was found that the mean value of α varied from a maximum value at the upper side of the inclined pipe to a minimum value at the lower side, and that the rate of decrease of this mean value of α with distance in the −Y direction became greater as the pipe inclination angle θ from the vertical was increased. It was also found that the mean value of uo was greatest at the upper side of the inclined pipe and decreased towards the lower side of the inclined pipe, the rate of decrease with distance in the −Y direction again becoming greater as θ was increased. For θ=45o, a water volumetric flow rate Qw=16.38m3h−1, an oil volumetric flow rate Qo=6.0m3h−1 and using a sampling period T=0.05s over a total time interval of 60s, it was found that at the upper side of the inclined pipe the standard deviation in uo was 31.6% of the mean value of uo. Furthermore for T=0.05s, θ=30o, Qw=16.38m3h−1 and Qo=6.0m3h−1 it was found that the standard deviation in the cross-pipe oil velocity component uY was approximately equal to the standard deviation in the axial velocity component uo. These large temporal variations in the local flow properties have been attributed to the presence of large scale Kelvin–Helmholtz waves which intermittently appear in the flow. It is believed that the techniques outlined in this paper for measuring the standard deviation of local flow properties as a function of the sampling period T will be of considerable value in validating mathematical models of time dependent oil–water flows. It should be noted that the principal focus of this paper is on the measurement techniques that were used and the methods of data analysis rather than the presentation of exhaustive experimental results at numerous different flow conditions.

Hydraulic efficiency and safety of vascular and non-vascular components in Pinus pinaster leaves

Leaves, the distal section of the soil-plant-atmosphere continuum, exhibit the lowest water potentials in a plant. In contrast to angiosperm leaves, knowledge of the hydraulic architecture of conifer needles is scant. We investigated the hydraulic efficiency and safety of Pinus pinaster needles, comparing different techniques. The xylem hydraulic conductivity (k(s)) and embolism vulnerability (P(50)) of both needle and stem were measured using the cavitron technique. The conductance and vulnerability of whole needles were measured via rehydration kinetics, and Cryo-SEM and 3D X-ray microtomographic observations were used as reference tools to validate physical measurements. The needle xylem of P. pinaster had lower hydraulic efficiency (k(s) = 2.0 × 10(-4) m(2) MPa(-1) s(-1)) and safety (P(50) = - 1.5 MPa) than stem xylem (k(s) = 7.7 × 10(-4) m(2) MPa(-1) s(-1); P(50) = - 3.6 to - 3.2 MPa). P(50) of whole needles (both extra-vascular and vascular pathways) was - 0.5 MPa, suggesting that non-vascular tissues were more vulnerable than the xylem. During dehydration to - 3.5 MPa, collapse and embolism in xylem tracheids, and gap formation in surrounding tissues were observed. However, a discrepancy in hydraulic and acoustic results appeared compared with visualizations, arguing for greater caution with these techniques when applied to needles. Our results indicate that the most distal parts of the water transport pathway are limiting for hydraulics of P. pinaster. Needle tissues exhibit a low hydraulic efficiency and low hydraulic safety, but may also act to buffer short-term water deficits, thus preventing xylem embolism.

Damage in needle tissues after infection with Chrysomyxa rhododendri increases cuticular conductance of Picea abies in winter

Chrysomyxa rhododendri is a rust which infects Picea abies growing near the alpine timberline. Attacked needles are normally shed, but few remain on shoots. We hypothesised that these needles increase transpiration of Picea during winter. Partly damaged, completely damaged and healthy needles of an infected tree as well as healthy needles of a resistant tree were compared in a microscopy analysis, and needle conductance of shoots was measured gravimetrically. Despite needle shedding, more than 6% of needles remaining on infected tree shoots were damaged. Partly damaged needles showed local brownish areas in the periphery and completely damaged needles necrotic parenchyma and epidermal tissues. Cuticular conductance of affected shoots was up to 25.23 +/- 2.75 mmol m(-2) s(-1) at moderate water potential and thus twofold higher than in the resistant tree. Needle shedding reduces negative effects of Chrysomyxa infections during summer, but remaining damaged needles impair tree water relations in winter.

Seasonal variations in monoterpene profiles and ecophysiological traits in Mediterranean pine species of group “halepensis”

Abstract: Foliar and cortical terpene profile, and needle gas exchange and water potential of P. halepensis, P. brutia and P. eldarica were compared over three consecutive seasons (1996-1998) in an experimental plantation nearby Firenze (Italy). Terpene percentages in mature tissue (cortex and needle) did not change in response to water stress during summer period and remained stable through seasons and years. Terpene profiles were not affected by seasonal drought, and are thus valuable to characterize Mediterranean pine species of the group “halepensis”. There was a threshold-type response of maximum daily gas exchange to decreasing predawn water potential in all pines. Net photosynthesis and needle conductance were linearly related, regardless of the species.

Roles of hydroxyl radical generating/scavenging mechanisms in pseudo polluted dew in reducing the foliar CO 2 assimilation rate and biomass production of Japanese red pine ( Pinus densiflora Sieb. et Zucc.) seedlings

Hydroxyl radical ( OH) is one of the most highly reactive of all active oxygen species. Aqueous-phase OH is photochemically generated in polluted dew droplets on needle surfaces of Japanese red pine ( Pinus densiflora Sieb. et Zucc., an evergreen coniferous tree) in declining pine forests. In former studies, we examined the effects of OH-generating mist solutions, which simulate polluted dew droplets, on needle ecophysiological traits of pine seedlings. Two types of solution with different OH-generating sources, i.e. the photo-Fenton reaction (H 2O 2-Fe-oxalate) and photolysis of HONO and NO 2 − [N(III)], had contrasting functional effects on needle gas exchange characteristics even though they had similar OH photoformation rates. In the present study, we investigated the effects of OH-generating mist solutions containing mixed-sources of OH (photo-Fenton and N(III)) on needle gas exchange and the biomass production of potted Japanese red pine seedlings. The N(III) and H 2O 2 concentrations of the OH-generating mist solutions were regulated to the same concentration (0, 25, 50, 75 or 100 μM, pH 5.2–5.3). Treated needles with the lowest photoformation rate and scavenging rate constant of OH had the smallest CO 2 assimilation rate ( A max) and needle conductance ( g n) of all treated pine seedlings. This suggests that the photoformation rate and scavenging rate constant do not always explain the ecophysiological disorders of pine needles subjected to OH-generating wet deposition. On the other hand, the calculated steady-state concentration of OH in the mist solutions was significantly negatively correlated with A max. In addition, pine seedlings with reduced A max showed suppressed biomass (dry weight) production. These results suggest that the OH concentration in the mist solutions is the decisive factor in explaining the negative effects of pseudo dew droplets not only on needle but also plant productivity. We propose that the deposition of polluted dew with chemical components and systems that result in a high OH concentration is one of the causes of growth decline of Japanese red pine.

Seasonal variations in monoterpene profiles and ecophysiological traits in Mediterranean pine species of group “halepensis”

Abstract: Foliar and cortical terpene profile, and needle gas exchange and water potential of P. halepensis, P. brutia and P. eldarica were compared over three consecutive seasons (1996-1998) in an experimental plantation nearby Firenze (Italy). Terpene percentages in mature tissue (cortex and needle) did not change in response to water stress during summer period and remained stable through seasons and years. Terpene profiles were not affected by seasonal drought, and are thus valuable to characterize Mediterranean pine species of the group “halepensis”. There was a threshold-type response of maximum daily gas exchange to decreasing predawn water potential in all pines. Net photosynthesis and needle conductance were linearly related, regardless of the species.

Altitudinal change in needle water relations of Pinus canariensis and possible evidence of a drought-induced alpine timberline on Mt. Teide, Tenerife

The alpine timberline on Mt. Teide, Tenerife, Canary Islands, occurs at about 1000 m lower elevation than that of continental mountains of similar latitude. We tested the hypothesis that edaphic and/or climatic drought are major causes for the timberline depression on this high subtropical island. Comparative measurements of soil water content, needle water status, stomatal conductance, carbon isotope signature, and foliar N concentration were conducted in mature trees of the timberline species, Pinus canariensis Chr. Smith ex. DC, along an elevational transect (upper montane forest at 1600 m to the timberline at 2100 m) in the wet and early dry seasons. The topsoil (0–10 cm) desiccated completely in the dry season at the timberline but retained a significantly higher soil moisture at 1600 m where cloud cover is frequent. Daily maximal stomatal conductance of pine needles decreased significantly from 1600 to 2100 m in the wet season which coincided with a higher water vapour saturation deficit of the air and a drier topsoil at 1800 and 2100 m compared to 1600 m. The δ 13 C value of sun-lit pine needles increased by 4.5 km −1 from 1600 to 2100 m, which is a greater increase with elevation than has been found in mountains on continents; this may indicate partial stomatal closure in high elevation pines. Daily minima of needle water potential in the dry season increased from –1.0 to – 1.5 MPa at 1600 m to –2.0 to –2.5 MPa at 1800 and 2100 m. We conclude that mature P. canariensis trees at the timberline are seasonally affected by edaphic and probably climatic drought which contradicts the opinion that drought stress is principally of low importance in alpine timberline environments. However, neither drought nor frost stress are likely to cause a critical reduction in vitality and growth rate of mature pines because the uppermost pine trees grow up to 14 m in height and have a vital appearance. The carbon sink limitation hypothesis is not applicable to the isolated pine trees at the timberline because self-shading and, consequently, lowered root zone temperatures do not occur. We forward the ‘multiple limitations at the seedling stage hypothesis’ in order to explain the timberline depression as resulting from a failure of P. canariensis toregenerate successfully at high elevations.

Minimum cuticular conductance and cuticle features of Picea abies and Pinus cembra needles along an altitudinal gradient in the Dolomites (NE Italian Alps).

Winter desiccation is believed to contribute to stress in coniferous trees growing at the treeline because cuticular conductance increases with altitude. To test whether winter desiccation occurs in high-altitude conifers of the Dolomites (NE Italian Alps), we measured minimum cuticular conductance (g(min)), needle wettability (contact angle) and cuticle thickness in Picea abies (L.) Karst. and Pinus cembra L. needles from December to August. Samples were collected from adult trees along an altitudinal gradient from valley bottom (1050 m a.s.l.) to the treeline (2170 m a.s.l.). The treeline site is one of the highest in the area and is characterized by a generally low wind exposure. Altitude had no effect on g(min) in either species. In P. abies, large seasonal variations in g(min) were recorded but no changes were related to needle age class. Pinus cembra had a low g(min) and appeared to be efficient in reducing needle water losses. There was a significant increase in g(min) with needle aging in P. cembra growing at low altitude that could be related to a shorter needle longevity compared with P. abies. High contact angles (> 110-120 ) suggested the presence of tubular epicuticular waxes on needles of both species. Contact angles were higher (low wettability) in high-altitude needles than in low-altitude needles. By the end of winter, there was no difference in contact angles between needles in the windward and leeward positions. Wax structures transformed toward planar shapes as demonstrated by the decrease in contact angle from winter to summer. In both species, the cuticle was thicker in needles of high-altitude trees than in needles of low-altitude trees and there was no correlation between g(min) and cuticle thickness. Because desiccation resistance did not decrease with altitude in either species, we conclude that they are not susceptible to winter desiccation at the tree line.

Influence of nitrogen and phosphorous availability and ozone stress on Norway spruce seedlings.

Four-year-old Norway spruce (Picea abies L. (Karst.)) seedlings were exposed to ambient and elevated (1.5 x ambient in 1997 and 1.6 x ambient in 1998) ozone concentrations [O3] and three nitrogen (N) and two phosphorus (P) availabilities: "optimal" values (control); 70% of the control N and P values (LN and LP); and 150% of the control N value (HN). Treatments were applied in an open-field ozone fumigation facility during the 1997 and 1998 growing seasons. Effects on growth, mineral and pigment concentrations, stomatal conductance and ultrastructure of needles were studied. The HN treatment increased growth significantly, whereas elevated [O3] had a slight or variable impact on growth and biomass allocation in all N treatments. Although there were no significant effects of the LP treatment on plant growth during the second year, there was a reduction in 1-year-old shoot dry mass in the elevated O3 + LP treatment at the end of the experiment. There were no significant treatment effects on mineral concentrations of current-year and 1-year-old needles at the final harvest. In response to the HN treatment, chlorophyll a and b and carotenoid concentrations increased significantly in current-year needles. Chlorophyll a/b ratio decreased in response to elevated [O3] alone, but increased in seedlings in the O(3) + LP treatment. Stomatal conductance of current-year needles decreased with increasing N availability, but increased in response to elevated [O3]. However, the O3-induced increase in stomatal conductance was less in the LN and LP treatments than in the control treatment. In chloroplasts of current-year needles, increased N availability decreased mean starch grain area, but increased the number of plastoglobuli. We conclude that Norway spruce seedlings are relatively tolerant to slightly elevated [O3], and that nitrogen and phosphorus imbalances do not greatly affect the influence of O3 on this species when the exposure lasts for two growing seasons or less.

Genotypic variation in carbon isotope discrimination and gas exchange of ponderosa pine seedlings under two levels of water stress

As part of a program to select ponderosa pine (Pinus ponderosa Dougl. ex Laws.) genotypes for improved drought tolerance, we examined physiological and morphological characteristics of 12 half-sib families of ponderosa pine from four seed sources; New Mexico, South Dakota, Nebraska, and Wyoming. We analyzed genetic variation in carbon isotope discrimination (Δ), photosynthetic gas exchange, needle morphology, and growth of 2-year-old seedlings from the four seed sources grown under two levels of moisture availability. To gain a better understanding of within-provenance variation and identify opportunities to refine selection strategies, we also examined family within seed source variation in the traits. Water stress significantly (P < 0.05) reduced net photosynthesis (A), needle conductance to water vapor (gwv), carbon isotope discrimination (Δ), and growth of the seedlings as compared to well-watered seedlings. However, instantaneous water use efficiency (A/gwv) did not differ between water treatments. Seedlings from New Mexico had significantly lower gwv and higher A/gwv than seedlings from the other sources. Carbon isotope discrimination was lowest for seedlings from New Mexico and Nebraska. Families within seed sources varied significantly in A, gwv, stomatal density, needle length, height increment, and Δ. Carbon isotope discrimination was significantly correlated with gwv but not with A, supporting results from mature trees suggesting that variation in Δ in ponderosa pine is more related to gwv than to A. Seed source × water treatment interactions were not observed for any of the traits analyzed. These results support our previous assertion that genotype × environment interaction in Δ of mature ponderosa pine trees from these sources grown in Nebraska and Oklahoma was related to factors other than moisture availability.

Simulations of stomatal conductance and ozone uptake to Norway spruce saplings in open-top chambers

A simulation model was developed to estimate the stomatal conductance and ozone flux to Norway spruce saplings in open-top chambers. The model was parameterized against needle conductance measurements that were made on 4–6-year-old spruce saplings, grown in open-top chambers, in July–September during three different seasons. The spruce saplings were either maintained well watered or subject to a 7–8 week drought period in July–September each year. The simulated conductance showed a good agreement with the measured conductance for the well-watered as well as the drought stress-treated saplings. The simulations were significantly improved when different vapour pressure deficit (VPD) functions were applied for well-watered and drought-stressed spruce saplings. The cumulated ozone uptake which was calculated from the conductance simulations showed less variation between years, compared to the cumulative ozone exposure index AOT40 (accumulated exposure over a threshold of 40 ppb or nl l−1) for the corresponding time periods. Measurements in May 1995 demonstrated the occurrence of long-term ‘memory-effects’ from the drought stress treatments on the conductance. Memory-effects need to be considered when simulation models for stomatal conductance are to be applied to long-lived forest trees under a multiple stress situation.

Provenance variation in carbon isotope discrimination of mature ponderosa pine trees at two locations in the Great Plains

We analyzed genotypic variation in carbon isotope discrimination (Δ), photosynthetic gas exchange, and needle morphology of ponderosa pine (Pinus ponderosa Dougl. ex Laws.) trees from four seed sources growing in two 26-year-old provenance plantings near Plattsmouth, Neb., and Norman, Okla. The populations studied were from South Dakota, New Mexico, Wyoming, and Nebraska. Net photosynthesis (A) and needle conductance to water vapor (gwv), were measured during the growing season of 1994. Specific leaf area, stomatal density, and Δ were analyzed in needles grown from 1991 to 1994. The southernmost source (New Mexico) had the highest intrinsic water-use efficiency (A/gwv) among the sources studied. Carbon isotope discrimination was correlated with growth (r = -0.81, P < 0.05), A/gwv (r < -0.54, P < 0.001), and gwv (r > 0.46, P < 0.05) but not A. Variation in Δ was significant among seed sources and years (P < 0.001). We observed a strong genotype × environment interaction in Δ resulting from geographic location but not moisture availability within locations. We hypothesize that the genotype × environment interaction is related to variation in growth phenology among the seed sources. Improving water-use efficiency or growth of ponderosa pine via Δ will require an understanding in genotypic variation in growth rhythms.

Provenance variation in carbon isotope discrimination of mature ponderosa pine trees at two locations in the Great Plains

We analyzed genotypic variation in carbon isotope discrimination (Δ), photosynthetic gas exchange, and needle morphology of ponderosa pine (Pinus ponderosa Dougl. ex Laws.) trees from four seed sources growing in two 26-year-old provenance plantings near Plattsmouth, Neb., and Norman, Okla. The populations studied were from South Dakota, New Mexico, Wyoming, and Nebraska. Net photosynthesis (A) and needle conductance to water vapor (gwv), were measured during the growing season of 1994. Specific leaf area, stomatal density, and Δ were analyzed in needles grown from 1991 to 1994. The southernmost source (New Mexico) had the highest intrinsic water-use efficiency (A/gwv) among the sources studied. Carbon isotope discrimination was correlated with growth (r = -0.81, P < 0.05), A/gwv (r < -0.54, P < 0.001), and gwv (r > 0.46, P < 0.05) but not A. Variation in Δ was significant among seed sources and years (P < 0.001). We observed a strong genotype × environment interaction in Δ resulting from geographic location but not moisture availability within locations. We hypothesize that the genotype × environment interaction is related to variation in growth phenology among the seed sources. Improving water-use efficiency or growth of ponderosa pine via Δ will require an understanding in genotypic variation in growth rhythms.

Diurnal changes in water conduction in loblolly pine (Pinus taeda) and Virginia pine (P. virginiana) during soil dehydration.

We studied diurnal changes in water conduction during soil dehydration in 37-month-old seedlings of one Virginia pine (Pinus virginiana Mill.) and two loblolly pine (P. taeda L.) sources, one from North Carolina (NC) and the other from the "Lost Pines" areas of Texas (TX), in an environmentally controlled growth chamber. For seedlings of similar biomass, the TX source had higher values of transpiration, needle conductance, and plant hydraulic conductivity under well-watered conditions than the NC source. Under dry soil conditions, the TX source had lower values of water conduction than the NC source. The Virginia pine source responded similarly to the TX source under both well-watered and dry soil conditions. For all three pine sources, gradients between soil and needle water potentials were greatest when the seedlings were moderately stressed. The TX and Virginia pine sources had higher gradients and lower daytime needle water potentials under moderate stress conditions than the NC source. Predawn needle water potentials did not differ among the pine sources. We conclude that the TX and Virginia pine sources have decreased daytime needle water potentials and increased water potential gradients during the daytime under moderate stress conditions, but with no disruption of recovery at predawn. The greater rates of transpiration and water conduction by the TX source compared with the NC source under well-watered conditions suggest a means by which growth can be maximized prior to the onset of drought, thereby enhancing survival of loblolly pines in drought-prone environments.

Genetic variation in summer gas exchange patterns of interior spruce (Picea glauca (Moench) Voss times Picea engelmannii Parry ex Engelm.)

Interior spruce (Picea glauca (Moench) Voss x Picea engelmannii Parry ex Engelm.) somatic seedlings from a range of clones were measured for gas exchange processes in relation to summer atmospheric parameters. Carbon isotope composition ( delta 13C) of needles was compared with gas exchange parameters and intrinsic water use efficiency (WUEi). Needle conductance (gwv) decreased as vapour pressure deficit (VPD) increased. Clone T703 had the lowest gwv level across all VPD conditions, with clones G351, N366, and W460 having significantly greater gwv than clone T703 at VPD levels &lt;2.0 kPa. Response of net photosynthesis (Pn) to photosynthetically active radiation (PAR) for all clones showed Pn to increase to PAR levels of around 1000 µmol ·m-2 ·s-1 and then, Pn rates were stable at higher PAR levels. Clones N366 and W460 had significantly greater Pn than clone T703 at all PAR levels. All clones showed Pn to decline as VPD increased, with clone W460 having the highest predicted Pn level across all VPD conditions and significantly greater Pn than clone T703. Higher WUEi was related to an increase in delta 13C, with clone T703 having the highest WUEi and delta 13C, followed by clone W460. Changes in delta 13C, for all clones, indicated a stronger relationship with gwv than with Pn.

Growth and Physiological Responses of Young Loblolly Pine Stands to Thinning

Abstract Although yield responses to thinning in loblolly pine have been well documented, short- and long-term morphological and physiological changes are largely unknown. To investigate these responses, three 8-yr-old, 0.2024 ha replicate stands of loblolly pine were thinned by 50% in May 1988, and bole growth and morphological changes in the crown were measured annually for the next 6 yr. In situ gas exchange measurements were monitored in the upper and lower third of the crowns monthly during the third through sixth growing seasons following thinning. First- and second-year physiological responses to thinning have been reported earlier (Ginn et al. 1991). Thinning slowed live crown recession and increased crown diameters resulting in increased photosynthetic surface area per tree. Over the 6 yr study period, the crown diameters of thinned trees increased 82% while the crown diameters of control trees increased only 20%. Larger bole diameters were observed for thinned trees four growing seasons after thinning. Photosynthetic rate and needle conductance did not increase overall, but were increased in the lower crown foliage of thinned trees. Needle dark respiration was higher in thinned stands in both the upper and lower crowns. Increased bole diameter growth is likely the result of the large increases in foliar biomass as well as initially higher physiological activity in the lower crowns of thinned stands. For. Sci. 43(4):529-534.

Performance of interior spruce seedlings treated with abscisic acid analogs

This research examined the performance of interior spruce (Piceaglauca (Moench) Voss × Piceaengelmannii Parry ex Engelm.) seedlings, each group treated with one of nine abscisic acid (ABA) analogs, during the initial stages of seedling establishment under a range of environmental conditions. Interior spruce seedlings were removed from frozen storage, ABA analog treatments were immediately applied, and seedlings were tested under low root temperature or moderate drought cycle conditions. Alternatively, seedlings were removed from frozen storage and held until bud break had occurred before ABA analog treatments were applied. These seedlings were then tested under severe drought or optimum environmental conditions. ABA analog 1, followed by ABA analog 2, had the most consistent performance of the nine tested ABA analogs under all combinations of environmental test conditions. These ABA analogs reduced needle conductance for 7–9 days when seedlings were tested under low root temperature conditions with only a reduction in net photosynthesis on the first day of testing. During three successive moderate drought cycles, seedlings treated with ABA analogs 1 and 2 had partial stomatal closure, thereby increasing mean shoot water potential by around 50%. During a severe drought, ABA analog 1 caused partial stomatal closure, which allowed seedlings to maintain a mean shoot water potential of greater than −3.0 MPa and a positive net photosynthesis up to 8 days longer than control seedlings. Under optimum environmental conditions, ABA analogs 1 and 2 reduced needle conductance for up to 7 days, with net photosynthesis reduced for 1 day. Root growth was not adversely affected in seedlings treated with any of the ABA analogs prior to bud break. However, when seedlings were treated after bud break, all ABA analogs reduced growth of long roots (&gt;4.0 cm) by approximately 60%. ABA analogs 1 and 2 delayed bud break by 4 days, when compared with control seedlings. Results are discussed in reference to the establishment process of spruce seedlings on reforestation sites.