Nearshore Marine Environment Research Articles

Diffusive Gradients in Thin Films Can Predict the Toxicity of Metal Mixtures to Two Microalgae: Validation for Environmental Monitoring in Antarctic Marine Conditions.

Anthropogenic contamination in the Antarctic near-shore marine environment is a challenge for environmental managers because of its isolation, high costs associated with monitoring and remediation activities, and the current lack of Antarctic-specific ecotoxicological data. The present study investigated the application of diffusive gradients in thin films (DGT) with a Chelex-100 binding resin for metal contaminant assessment in Antarctic marine conditions. Diffusion coefficients for cadmium (Cd), copper (Cu), nickel (Ni), lead (Pb), and zinc (Zn), determined at 1 °C, ranged between 2.1 and 2.6×10-6 cm2 /s and were up to 32% lower than those derived by theoretical calculations. Competition of metals on the DGT binding resin was observed at subsaturation concentrations, reducing the effective capacity for metal uptake by approximately 60%. The lability of the dissolved (0.45 µm filterable) Cd, Cu, Pb, and Zn metal fraction to DGT was generally >90% and unaffected by the presence of the Antarctic marine microalga Phaeocystis antarctica. Both DGT and dissolved metal concentrations gave equivalent mixture toxicity predictions in independent action and concentration addition models to P. antarctica and Cryothecomonas armigera; that is, predictions using DGT-labile concentrations also showed antagonism to P. antarctica, which agrees with previously determined mixture interactivity. The benefits of DGT over traditional sampling techniques (i.e., discrete water sampling) include lower method detection limits (MDLs), in situ assessment, and time-averaged concentrations which capture pulses of contamination typical of the Antarctic near-shore marine environment. The present study provides MDLs and recommended minimum deployment times to guide field deployments in Antarctica. Environ Toxicol Chem 2019;38:1323-1333. © 2019 SETAC.

Wind Speed Estimation Using Acoustic Underwater Glider in a Near-Shore Marine Environment

This paper investigates the use of an acoustic glider to perform acoustical meteorology. This discipline consists of analyzing ocean ambient noise to infer above-surface meteorological conditions. The paper focuses on wind speed estimation, in a near-shore marine environment. In such a shallow water context, the ambient noise field is complex, with site-dependent factors and a variety of nonweather concurrent acoustic sources. A conversion relationship between sound pressure level and wind speed is proposed, taking the form of an outlier-robust nonlinear regression model learned with in situ data. This method is successfully applied to experimental data collected in Massachusetts Bay (MA, USA) during four glider surveys. An average error in wind speed estimation of 1.3 m · s <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> (i.e., average relative error of 14%) over wind speed values up to 17 m · s <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">-1</sup> is reported with this method, which outperformed results obtained with relationships from the literature. Quantitative results are also detailed on the dependence of wind speed error estimation on the environment characteristics, and on the classification performance of observations contaminated by acoustic sources other than wind. Passive acoustic-based weather systems are a promising solution to provide long-term in situ weather data with fine time and spatial resolutions. These data are crucial for satellite calibration and assimilation in meteorological models. From a broader perspective, this paper is the first step toward an operationalization of acoustic weather systems and their on-board embedding in underwater monitoring platforms such as gliders.

Recruitment tolerance to increased temperature present across multiple kelp clades.

Kelp systems dominate nearshore marine environments in upwelling zones characterized by cold temperatures and high nutrients. Worldwide, kelp population persistence and recruitment success generally decreases with rising water temperatures coupled with low nutrients, making kelp populations vulnerable to impending warming of the oceans. This response to climate change at a global scale, however, may vary due to regional differences in temperature variability, acclimation, and differential responses of kelp species to changing conditions. Culture experiments were conducted on 12 eastern Pacific kelp taxa across geographic regions (British Columbia, central California, and southern California) under three nitrate levels (1,5,and 10μmol/L) and two temperatures (12°C and 18°C) to determine sporophyte production (i.e., recruitment success). For all taxa from all locations, sporophytes were always present in the 12°C treatment and when recruitment failure was observed, it always occurred at 18°C, regardless of nitrate level, indicating that temperature is the driving factor limiting recruitment, not nitrate. Rising ocean temperatures will undoubtedly cause recruitment failure for many kelp species; however, the ability of species to acclimatize or adapt to increased temperatures at the warmer edge of their species range may promote a resiliency of kelp systems to climate change at a global scale.

Acoustic monitoring reveals the times and tides of harbor porpoise (Phocoena phocoena) distribution off central Oregon, U.S.A.

AbstractHarbor porpoises (Phocoena phocoena) are commonly observed in Oregon's nearshore marine environment yet knowledge of their ecosystem use and behavior remains limited, generating concerns for potential impacts on this species from future coastal development. Passive acoustic monitoring was used to investigate spatial and temporal variations in the presence and foraging activity of harbor porpoises off the Oregon coast from May through October 2014. Digital monitoring devices (DMONs) were deployed to record acoustic data (320 kHz sample rate) in two neighboring but bathymetrically different locations off the Oregon coast: (1) a site on the 30 m isobath in close proximity (&lt;50 m) to a rocky reef, and (2) a site on the 60 m isobath in an open sandy environment. Data were analyzed with respect to two dynamic cyclic variables: diel and tidal phase. Porpoise presence at the rocky reef site was aligned with the ebb phase of the tidal forcing, while, harbor porpoise presence and foraging at the offshore, sandy bottom site was associated with night‐time foraging. The spatial and temporal patterns identified in this study suggest harbor porpoise habitat use is modulated by specific environmental conditions particular to each site that maximize foraging efficiency.

Microplastics in juvenile Chinook salmon and their nearshore environments on the east coast of Vancouver Island

Microplastics are a significant issue in the world's oceans. These small plastic particles (<5 mm in size) are becoming globally ubiquitous in the marine environment and are ingested by various fish species. Here we investigate the incidence of microplastics in juvenile Chinook salmon and their nearshore marine environments on the east coast of Vancouver Island, British Columbia. We completed a series of beach seines, plankton tows and sediment cores in nearshore areas of importance to juvenile salmon. Microplastics were extracted from fish, water and sediment samples and concentrations were quantified. Microplastics analysis, consisting predominantly of fibrous plastics, showed juvenile Chinook salmon contained 1.2 ± 1.4 (SD) microplastics per individual while water and sediment samples had 659.9 ± 520.9 microplastics m−3 and 60.2 ± 63.4 microplastics kg−1 dry weight, respectively. We found no differences in microplastic concentrations in juvenile Chinook and water samples among sites but observed significantly higher concentrations in sediment at the Deep Bay site compared to Nanaimo and Cowichan Bay sites. Chinook microplastic concentrations were relatively low compared to literature values and, given the size and type of microplastics we observed, are unlikely to represent an immediate threat to fish in this area. However, microplastics less than 100 μm in size were not included in the study and may represent a greater threat due to their ability to translocate through tissues.

MARINE MANAGEMENT AND THE SITING OF ELECTRICAL GENERATING STATIONS ON TROPICAL SHORELINES

Expanding utilization of electricity by developing tropical nations will produce increasing amounts of waste heat that will, in most cases, be disposed into natural water systems. Such disposal represents a serious potential marine management problem for nations which intensively utilize the nearshore marine environment in their subsistence economy. This communication reviews the marine environmental effects resulting from thermal disposal at three generating stations on the Island of Oahu, Hawaii and compares these with results of similar studies made elsewhere in the tropics and subtropics. Conclusions are drawn from these studies concerning desirable environmental features to be considered for thermal outfalls in tropical areas, and some environmental consequences of alternative methods of cooling water treatment are discussed.

Ichnology and depositional environments of the Upper Ordovician Stony Mountain Formation in the Williston Basin, Canada: Refining ichnofacies and ichnofabric models for Epeiric Sea carbonates

Ordovician epeiric sea carbonates in intracratonic basins of Laurentia are enigmatic due to their unique depositional settings and the absence of modern analogs. Integrated ichnologic and sedimentologic analysis of the Upper Ordovician Stony Mountain Formation in the Williston Basin of Canada allows recognition of neritic-marine, nearshore-marine, open-lagoon, restricted-lagoon, peritidal sand-shoal and peritidal-flat subenvironments. The Cruziana ichnofacies occurs in neritic (between fair-weather and storm wave bases) and nearshore (around fair-weather wave base) marine environments. The depauperate Cruziana ichnofacies is present in open- and restricted-lagoon environments, indicating a shift from fully marine to stressed conditions. In the open lagoon, composite ichnofabrics related to omission surfaces illustrate the low rates of background sedimentation interrupted by event deposition and early cementation, illustrating the Glossifungites and Trypanites ichnofacies. The decreased size of discrete burrows in the restricted lagoon is attributed to reduced oxygenation under stagnation rather than hypersalinity. The peritidal complex includes high-energy sand shoals and low-energy tidal flats. Only monospecific colonization took place sporadically in associated subtidal environments within the peritidal complex deposits.

Defining wet season water quality target concentrations for ecosystem conservation using empirical light attenuation models: A case study in the Great Barrier Reef (Australia)

Optically active water quality components (OAC) transported by flood plumes to nearshore marine environments affect light levels. The definition of minimum OAC concentrations that must be maintained to sustain sufficient light levels for conservation of light-dependant coastal ecosystems exposed to flood waters is necessary to guide management actions in adjacent catchments. In this study, a framework for defining OAC target concentrations using empirical light attenuation models is proposed and applied to the Wet Tropics region of the Great Barrier Reef (GBR) (Queensland, Australia). This framework comprises several steps: (i) light attenuation (Kd(PAR)) profiles and OAC measurements, including coloured dissolved organic matter (CDOM), chlorophyll-a (Chl-a) and suspended particulate matter (SPM) concentrations collected in flood waters; (ii) empirical light attenuation models used to define the contribution of CDOM, Chl-a and SPM to the light attenuation, and; (iii) translation of empirical models into manageable OAC target concentrations specific for wet season conditions. Results showed that (i) Kd(PAR) variability in the Wet Tropics flood waters is driven primarily by SPM and CDOM, with a lower contribution from Chl-a (r2 = 0.5, p < 0.01), (ii) the relative contributions of each OAC varies across the different water bodies existing along flood waters and strongest Kd(PAR) predictions were achieved when the in-situ data were clustered into water bodies with similar satellite-derived colour characteristics (‘brownish flood waters’, r2 = 0.8, p < 0.01, ‘greenish flood waters’, r2 = 0.5, p < 0.01), and (iii) that Kd(PAR) simulations are sensitive to the angular distribution of the light field in the clearest flood water bodies. Empirical models developed were used to translate regional light guidelines (established for the GBR) into manageable OAC target concentrations. Preliminary results suggested that a 90th percentile SPM concentration of 11.4 mg L−1 should be maintained during the wet season to sustain favourable light levels for Wet Tropics coral reefs and seagrass ecosystems exposed to ‘brownish’ flood waters. Additional data will be collected to validate the light attenuation models and the wet season target concentration which in future will be incorporated into wider catchment modelling efforts to improve coastal water quality in the Wet Tropics and the GBR.

Late Triassic (early Carnian–Norian) palynology of the Sentralbanken High, Norwegian Barents Sea

ABSTRACTFour stratigraphic cores (7533/2-U-1, 2-U-2, 3-U-7 and 7534/4-U-1) drilled in the Sentralbanken High provide important reference sections for the Upper Triassic succession in the northern Barents Sea. The cores span the De Geerdalen, Flatsalen and Svenskøya formations (Kapp Toscana Group), which are equivalent to the Snadd to middle Fruholmen formations of the southern Barents Sea. Only cursory palynological study of the cores has been conducted previously, and no published palynofacies data is available. We present a detailed palynostratigraphy from the cores, and describe six informal palynological assemblages. Comparison with independently dated sections from elsewhere in the region enables a revised age interpretation of early Carnian to Norian for the cored interval, thereby improving correlation between Sentralbanken, Svalbard and the southern Barents Sea. A dominance of amorphous organic matter (AOM) and marine palynomorphs indicates a distal suboxic–anoxic marine depositional environment for the lower De Geerdalen Formation, with an abrupt change to a nearshore marine environment above an erosion surface in core 7534/4-U-1. Palynofacies from the remainder of the formation are characterised by a dominance of phytoclasts and are interpreted as indicative of a delta front to coastal plain environment. The reappearance of AOM, microforaminiferal test linings and marine microphytoplankton in the Flatsalen Formation in cores 7533/2-U-1 and 2-U-2 reflects a regional transgression of early Norian age. Reworked palynomorphs, shale rip-up clasts and a transition to phytoclast-dominated palynofacies above an erosion surface at the base of the overlying Svenskøya Formation is consistent with a disconformity, which was previously identified as a possible sequence boundary on the neighbouring island of Hopen.

Late Cretaceous marine arthropods relied on terrestrial organic matter as a food source: Geochemical evidence from the Coon Creek Lagerstätte in the Mississippi Embayment.

The Upper Cretaceous Coon Creek Lagerstätte of Tennessee, USA, is known for its extremely well-preserved mollusks and decapod crustaceans. However, the depositional environment of this unit, particularly its distance to the shoreline, has long been equivocal. To better constrain the coastal proximity of the Coon Creek Formation, we carried out a multiproxy geochemical analysis of fossil decapod (crab, mud shrimp) cuticle and associated sediment from the type section. Elemental analysis and Raman spectroscopy confirmed the presence of kerogenized carbon in the crabs and mud shrimp; carbon isotope (δ13 C) analysis of bulk decapod cuticle yielded similar mean δ13 C values for both taxa (-25.1‰ and -26‰, respectively). Sedimentary biomarkers were composed of n-alkanes from C16 to C36 , with the short-chain n-alkanes dominating, as well as other biomarkers (pristane, phytane, hopanes). Raman spectra and biomarker thermal maturity indices suggest that the Coon Creek Formation sediments are immature, which supports retention of unaltered, biogenic isotopic signals in the fossil organic carbon remains. Using our isotopic results and published calcium carbonate δ13 C values, we modeled carbon isotope values of carbon sources in the Coon Creek Formation, including potential marine (phytoplankton) and terrestrial (plant) dietary sources. Coon Creek Formation decapod δ13 C values fall closer to those estimated for terrigenous plants than marine phytoplankton, indicating that these organisms were feeding primarily on terrigenous organic matter. From this model, we infer that the Coon Creek Formation experienced significant terrigenous organic matter input via a freshwater source and thus was deposited in a shallow, nearshore marine environment proximal to the shoreline. This study helps refine the paleoecology of nearshore settings in the Mississippi Embayment during the global climatic shift in the late Campanian-early Maastrichtian and demonstrates for the first time that organic δ13 C signatures in exceptionally preserved fossil marine arthropods are a viable proxy for use in paleoenvironmental reconstructions.

Formation, Fate, and Impacts of Microscopic and Macroscopic Oil‐Sediment Residues in Nearshore Marine Environments: A Critical Review

AbstractCrude oil that is spilled in marine environments often interacts with suspended sediments to form residues that can impact the recovery of the affected nearshore ecosystems. When spilled oil and sediment interact, they can form either small microscopic aggregates, commonly referred to as oil‐particle aggregates, or large macroscopic agglomerates, referred to as sediment‐oil agglomerates or sediment‐oil mats. Although these different sized oil‐sediment residues have similar compositions, they are formed under different conditions and have different fates in nearshore environments; the goal of this review is to synthesize our current understanding of these two types of residues. We believe that researchers who focus solely on studying either microscopic aggregates or macroscopic agglomerates could benefit from understanding the research findings available in the other field. In this study, we compare and contrast various processes that control the formation, fate, and impacts of these two types of residues in nearshore environments and point out some of the knowledge gaps in this field. Additionally, these residues have been referred to by many names in the past, leading to confusion and misconceptions at times. In this effort, we recommend a uniform nomenclature to distinguish them based on their physical size. Our overall aim is to bridge the gap between microscopic and macroscopic oil‐sediment residue literature to foster a robust exchange of ideas, which we believe can lead to the development of efficient strategies for managing oil spills that affect nearshore environments.

Determination of water quality, toxicity and estrogenic activity in a nearshore marine environment in Rio de Janeiro, Southeastern Brazil

Endocrine disrupting compounds (EDCs) can be found in domestic sewage, wastewater treatment plant effluents, natural water, rivers, lakes and in the marine environment. Jurujuba Sound, located in the state of Rio de Janeiro, Southeastern Brazil, receives untreated sewage into its waters, one the main sources of aquatic contamination in this area. In this context, the aim of the present study was to evaluate the estrogenic potential of water sampled from different depths and from areas with differential contamination levels throughout Jurujuba Sound. Water quality was evaluated and acute toxicity assays using Allviibrio fischeri were conducted, while estrogenic activity of the water samples was determined by a Yeast Estrogen Screening assay (YES). Water quality was mostly within the limits established for marine waters by the Brazilian legislation, with only DOC and ammoniacal nitrogen levels above the maximum permissible limits. No acute toxicity effects were observed in the Allivibrio fisheri assay. The YES assay detected moderate estrogenic activity in bottom water samples from 3 sampling stations, ranging from 0.5 to 3.2ngL−1, as well as in one surface water sample. Estrogenic activity was most frequently observed in samples from the bottom of the water column, indicating adsorption of estrogenic compounds to the sediment.

Modeling sediment transport in Qatar: Application for coastal development planning.

Hydrodynamics and sediment transport are key physical processes contributing to habitat structure within the marine environment. Coastal development that results in the alteration of these processes (e.g., changing water flushing and/or sedimentation rates) can have detrimental impacts on sensitive systems. This is a current, relevant issue in Qatar as its coastal regions continue to be developed, not only around the capital of Doha, but in many areas around this Arabian Gulf peninsula. The northeastern Qatari coast is comprised of diverse and sensitive flora and fauna such as seagrass and macroalgae meadows, coral reefs and patches, turtles, and dugongs that tolerate harsh environmental conditions. In the near future, this area may see a rise in anthropogenic activity in the form of coastal development projects. These projects will add to existing natural stresses, such as high temperature, high salinity, and low rates of precipitation. Consequently, there is a need to characterize this area and assess the potential impacts that these anthropogenic activities may have on the region. In the present study, a novel sediment transport model is described and used to demonstrate the potential impact of altering hydrodynamics and subsequent sediment transport along the northeastern Qatar nearshore marine environment. The developed models will be tested using potential scenarios of future anthropogenic activities forecasted to take place in the area. The results will show the effects on water and sediment behavior and provide a scientific approach for key stakeholders to make decisions with respect to the management of the considered coastal zone. Furthermore, it provides a tool and framework that can be utilized in environmental impact assessment and associated hydrodynamic studies along other areas of the Qatari coastal zone. Integr Environ Assess Manag 2018;14:240-251. © 2017 SETAC.

Evolution of phenology in a salmonid population: a potential adaptive response to climate change

Accumulating evidence has indicated that many fish populations are responding to climate change through shifts in migration time, but genetic data identifying the role of evolution in these shifts are rare. One of the first demonstrations of evolution of migration time was produced by monitoring allozyme alleles that were experimentally manipulated to genetically mark late-migrating pink salmon (Oncorhynchus gorbuscha). Here, we extend that research by using observations of the marker alleles in fry to demonstrate that these changes in migration time were caused by directional selection against the late-migrating phenotype during the oceanic phase of the salmonid life cycle. The selective event, which appeared to be driven by early vernal warming of the nearshore marine environment and consequent decreased survival of late-migrating fry relative to early-migrating fry, decreased the late-migrating phenotype from more than 50% to approximately 10% of the total fry abundance in only one generation. These demographic changes have persisted over the subsequent 13 generations and suggest that a larger trend toward earlier migration time in this population may reflect adaptation to warming sea-surface temperatures.

Eighty years of food-web response to interannual variation in discharge recorded in river diatom frustules from an ocean sediment core

Little is known about the importance of food-web processes as controls of river primary production due to the paucity of both long-term studies and of depositional environments which would allow retrospective fossil analysis. To investigate how freshwater algal production in the Eel River, northern California, varied over eight decades, we quantified siliceous shells (frustules) of freshwater diatoms from a well-dated undisturbed sediment core in a nearshore marine environment. Abundances of freshwater diatom frustules exported to Eel Canyon sediment from 1988 to 2001 were positively correlated with annual biomass of Cladophora surveyed over these years in upper portions of the Eel basin. Over 28 years of contemporary field research, peak algal biomass was generally higher in summers following bankfull, bed-scouring winter floods. Field surveys and experiments suggested that bed-mobilizing floods scour away overwintering grazers, releasing algae from spring and early summer grazing. During wet years, growth conditions for algae could also be enhanced by increased nutrient loading from the watershed, or by sustained summer base flows. Total annual rainfall and frustule densities in laminae over a longer 83-year record were weakly and negatively correlated, however, suggesting that positive effects of floods on annual algal production were primarily mediated by "top-down" (consumer release) rather than "bottom-up" (growth promoting) controls.

Glacier shrinkage driving global changes in downstream systems

Glaciers cover ∼10% of the Earth’s land surface, but they are shrinking rapidly across most parts of the world, leading to cascading impacts on downstream systems. Glaciers impart unique footprints on river flow at times when other water sources are low. Changes in river hydrology and morphology caused by climate-induced glacier loss are projected to be the greatest of any hydrological system, with major implications for riverine and near-shore marine environments. Here, we synthesize current evidence of how glacier shrinkage will alter hydrological regimes, sediment transport, and biogeochemical and contaminant fluxes from rivers to oceans. This will profoundly influence the natural environment, including many facets of biodiversity, and the ecosystem services that glacier-fed rivers provide to humans, particularly provision of water for agriculture, hydropower, and consumption. We conclude that human society must plan adaptation and mitigation measures for the full breadth of impacts in all affected regions caused by glacier shrinkage.

Measuring Stable Carbon and Nitrogen Isotopes in Mytilus galloprovincialis to Elucidate the Sources of Organic Matter in Three Different Nearshore Marine Environments

ABSTRACT Rumolo, P.; Carannante, M.; Gherardi, S.; Tamburrino, S.; Vallefuoco, M.; Migliaccio, R.; D'Argenzio, C., and Barra, M., 2017. Measuring stable carbon and nitrogen isotopes in Mytilus galloprovincialis to elucidate the sources of organic matter in three different nearshore marine environments. Stable carbon and nitrogen isotopes (δ13C and δ15N) in mussels (Mytilus galloprovincialis) were used to elucidate the sources of sedimentary organic matter in nearshore marine environments at three different locations: (1) Naples harbour, (2) Formia harbour, and (3) Gulf of Gaeta (Tyrrhenian Sea). The sites showed different environmental characteristics, with the latter one considered as the control site, because it hosts “mussel farms” cultivated for human food. Significant differences in both δ13C and δ15N were found at Naples harbour compared to the other two sites. In particular, the lowest δ15N (>4‰) and the highest δ13C (≥1.6‰) values were found in mussels collected in Naples harbour, while similar δ1...

Ecological factors influencing lifetime productivity of pink salmon (Oncorhynchus gorbuscha) in an Alaskan stream

Ecological factors underlying freshwater productivity and marine survival of pink salmon (Oncorhynchus gorbuscha) were evaluated by analyzing a 30 year time series of local environmental data and censuses of migrating adult and juvenile fish collected at Auke Creek, Alaska. Freshwater productivity was influenced primarily by spawning habitat limitation and less so by stream temperature and flow. Furthermore, a trend of declining freshwater productivity was detected over the time series, which may be related to observed declines in spawning substrate quality and in the duration of the adult migration. Marine survival was highly variable among brood years and was influenced by physical conditions in the nearshore marine environment; warm sea-surface temperatures during nearshore residency were associated with higher marine survival rates, whereas high stream flows late in the fry emigration period were associated with reduced marine survival. Simulations of adult recruitment, based on ecological factors in the freshwater and marine environments, indicated that the productivity of pink salmon in this stream is determined primarily by early marine survival.

Establishing nearshore marine injuries for the Deepwater Horizon natural resource damage assessment using AQUATOX

The Oil Pollution Act of 1990 (OPA) requires that the responsible party make the public whole for natural resource injuries attributable to an oil spill incident. We have presented our approach at establishing natural resource baseline conditions for multiple habitats in Coastal Mississippi and Alabama. This paper presents the subsequent steps that were taken to estimate natural resource injuries for the Deepwater Horizon Oil release (DWH) using AQUATOX Release 3.1 NME (Nearshore Marine Environments). TPAH (Total polycyclic aromatic hydrocarbon) exposure matrices in water and sediment were estimated over a spatial gradient based on observed data in Mississippi and Alabama. Bioaccumulation parameters were derived from literature and reasonable bioaccumulation calibration was verified using site specific data when available. TPAH was segregated into six different analyte groups, binned by Kow, based on observed sediment data. Toxicity data from NOAA and literature were used to estimate effects of TPAH and individual analytes on species observed on site. Combining each of these analyses, total injuries could be estimated for Mississippi and Alabama ranging from 0.2% to 4.2% of secondary and tertiary productivity lost over three years, depending on the habitat investigated and its spatial location in the study area. The analysis demonstrates the effectiveness of the AQUATOX model as a tool to quantify levels of injury by comparing the results of a baseline ecological model, calibrated and verified with pre-oiling observations, with post-oiling results. This alternative approach to injury assessment can be used to validate single-species approaches and also to evaluate the injury to, and recovery of, an integrated ecosystem.

Evaluating Nitrogen-Containing Biosynthetic Products Produced by Saltwater Culturing of Several California Littoral Zone Gram-Negative Bacteria.

The biosynthetic potential of marine-sediment-derived Gram-negative bacteria is poorly understood. Sampling of California near-shore marine environments afforded isolation of numerous Gram-negative bacteria in the Proteobacteria and Bacteriodetes phyla, which were grown in the laboratory to provide extracts whose metabolites were identified by comparative analyses of LC-mass spectrometry and MSn data. Overall, we developed an assemblage of seven bacterial strains grown in five different media types designed to coax out unique secondary metabolite production as a function of varying culture conditions. The changes in metabolite production patterns were tracked using the GNPS MS2 fragmentation pattern analysis tool. A variety of nitrogen-rich metabolites were visualized from the different strains grown in different media, and strikingly, all of the strains examined produced the same new, proton-atom-deficient compound, 1-methyl-4-methylthio-β-carboline (1), C13H12N2S. Scale-up liquid culture of Achromobacter spanius (order: Burkholderiales; class: Betaproteobacteria) provided material for the final structure elucidation. The methods successfully combined in this work should stimulate future studies of molecules from marine-derived Gram-negative bacteria.