NC USA Research Articles

Serum antibody levels against select bacterial pathogens in Atlantic bottlenose dolphins, Tursiops truncatus, from Beaufort NC USA and Charleston Harbor, Charleston, SC, USA

Concern over the emergence of zoonotic diseases in marine organisms is growing. In response to this concern, this study set out to measure antibody activities against bacterial pathogens in Atlantic bottlenose dolphins, Tursiops truncatus, from the coastal estuaries of NC and SC, USA. Individuals from Charleston SC harbor, a heavily industrialized shipping harbor estuary, and from Beaufort NC, a non-shipping estuary, were examined. Purified IgG was obtained from pooled sera using ammonium sulfate precipitation steps and protein-G procedures, which was then used to generate a panel of IgG-specific monoclonal antibodies. Two of these antibodies, mAbs BB-10-2 (IgG 1) and BB-32-2 (IgG 2b), were then used to determine total serum IgG concentrations using a sandwich capture ELISA. Circulating IgG levels were variable between individuals and between the two pods. MAb BB-10-2 was then used in an indirect ELISA to determine serum antibody activities against several common marine bacteria as well as the human pathogens E. coli and E. coli strain 0157:H7, Vibrio parahemolyticus, V. vulnificus, V. cholerae, Mycobacteria marinum, M. fortuitum, and M. chelonae. The highest antibody activities were against mycobacteria, two of which are zoonotic pathogens. Males had the highest antibody activities, thus suggesting low cell-mediated immunity against intracellular pathogens in these individuals. T-cell proliferation in response to Con-A, an indicator of cell-mediated immune function, was then measured in the Beaufort population. Males had the lowest proliferation responses, however a negative correlation between antibody activities and T-cell proliferation in individuals could not be established for either of the Mycobacteria species. Overall, antibody activities against all bacteria, including innocuous species such as V. anguillarum, V. natrigens, and M. xenopi were highly variable between individual dolphins and the two pods, with some animals exhibiting very high activities. These studies suggests that dolphin populations should be monitored by following the health and seroprevalence of pathogens of interest in select individual animals over time.

Absence of atherosclerosis in intramyocardial coronary arteries and its relationship to mural stress: Robicsek, F., M.D., Thubrikar, M.J., Ph.D., Heineman Research Laboratory, Carolinas Medical Center, Charlotte, NC USA

Absence of atherosclerosis in intramyocardial coronary arteries and its relationship to mural stress: Robicsek, F., M.D., Thubrikar, M.J., Ph.D., Heineman Research Laboratory, Carolinas Medical Center, Charlotte, NC USA

48 Local perturbation of recombinant HIV-1 reverse transcriptase by urea occurs prior to global denaturation: LL Wright, JE Wilson, JL Martin, SE Haire, PH Ray, Painter, Division of Virology, Burroughts Wellcome Co, Research Triangle Park, NC USA

48 Local perturbation of recombinant HIV-1 reverse transcriptase by urea occurs prior to global denaturation: LL Wright, JE Wilson, JL Martin, SE Haire, PH Ray, Painter, Division of Virology, Burroughts Wellcome Co, Research Triangle Park, NC USA

The presence or absence of gp 120/gp 160 bands on indeterminate Western blota

King, R.; Frey, S.; Beisha, R.; de Perre, P. Van; Karita, E.; Allen, S. Author Information

Phase II intensive weekly treatment schedule with six drugs for extensive disease small cell lung cancer (SCLC): Galen L. Wampler and Diane Alt for the Mid-Atlantic Oncology Program. Richmond, VA and Chapel Hill, NC USA

Phase II intensive weekly treatment schedule with six drugs for extensive disease small cell lung cancer (SCLC): Galen L. Wampler and Diane Alt for the Mid-Atlantic Oncology Program. Richmond, VA and Chapel Hill, NC USA