Purpose: To find out whether there is difference of acupuncturemanipulation between two acupuncturistswhen they were stimulating the same acupoint in a same person with the same needling guidance, and analysis stability of acupuncture manipulation of them. Methods:Weused a cross-over design tomeasuremanipulation of acupuncturists and collect data. Two acupuncturists with different acupuncture experience (senior-A, junior-B) would give acupuncture treatment to cancer patients who receiving chemotherapy to control nausea andvomiting. Every patient were treated by A (or B) first and B (or A) second. T heir ordersweredetermined randomly. RN12 (ZhongWan)was selected and acupuncturists used evenmanipulatingmethods (ping bu ping xie) under the same guidance (depth, insertion angle, treatment duration). Eligible data of eight patients with 2-5 times treatment of every session were selected from this clinical trial data collection. Acusensor, a non-invasive optical sensor was used to collect data during the acupuncture treatments. Data of displacement frequency (DF), displacement amplitude (DA), rotation frequency (RF) and rotation amplitude (RA) was collected and compared. Results: 1. We used Paired samples test to find the difference. Mean was calculated to be compared. There were significant differences for DA(senior 6.93±3.25mm, junior 1.62±0.53mm,P<0.005) and RF(senior 0.43±0.07Hz,junior 0.60±0.16Hz,P =0.008). There were no significant differences for DF AND RA. 2. We used one-way ANOVA to measure the stability that grouped by acupoints and treatment days. Acupuncturistswere analyzed respectively. The result showed that there was no significant differences for DF, DA, RF and RA. Conclusion: Different acupuncturists do have manual manipulation differences. From data we can see that senior needling deeper and slower than junior. As for stability, we considered that both senior and junior had a stable acupuncture manipulation. Contact: Congcong Wang, wangcongcong08@sina.cn