In order to improve a naturally aspirated (NA) Compression Ignition (CI) engine operating on biodiesel fuel, this research proposes a Guide Vane Swirl and Tumble Device (GVSTD) inside the intake runner of a HINO W04D diesel engine to improve the in-cylinder air flow characteristic as an alternative solution to supplement existing techniques. When the air intake was guided in front of the intake port, organized turbulent air was generated, consequently assisting the fuel to evaporate and mix to produce better combustion and eventually produce better performance. To examine the effectiveness of the GVSTD, a 3D IC engine simulation model was developed. Additionally, this paper also investigates the optimal vane height of GVSTD of four vanes which twist at 35° clockwise direction and are arranged perpendicular to each other with the length of the vanes three times that of the intake radius. Ten simulation models of the GVSTD, where the vane height varied from 10% to 100% of the intake radius were prepared. The simulation results of in-cylinder pressure, turbulence kinetic energy and velocity of the base and all GVSTD models were then compared. The results illustrate a promising improvement of the in-cylinder air flow characteristics inside the fuel injected region by utilizing 20% vane height compared to standard model.