The environment inside airliner cabins has attracted increasing attention. Instantaneous airflow, as one of the fundamental parameters of airflow field, affects aspects of the airliner cabin environment such as occupant comfort and airborne disease transmission. This study evaluated the characteristics of instantaneous airflow in airliner cabins with different ventilation systems (mixing ventilation and displacement ventilation) and focused on the region above passengers' heads, where complex turbulence characteristics are found. This investigation used ultrasonic anemometers for measurements at ten selected points above passengers' heads. The energy ratio on the low-frequency scale with mixing ventilation was greater than that with displacement ventilation. The instantaneous airflow with mixing ventilation was close to that of natural wind, while the instantaneous airflow with displacement ventilation was close to that of mechanical wind. Compared with mixing ventilation, displacement ventilation produced a smaller vortex length and a shorter residence time. Moreover, there was no long-term vortex in the flow field under displacement ventilation, and therefore, pollutants would be discharged more quickly from the cabin. In addition, this paper distinguishes the spatial and temporal turbulence scales of mixing and displacement ventilation, thus providing a reference for the selection of grid size and time steps in computational fluid dynamics simulations.