Natural Settings Research Articles

Correlates of Co-Infection with Coccidiosis and Avian Malaria in House Finches (Haemorhous mexicanus).

Pathogens have traditionally been studied in isolation within host systems; yet in natural settings they frequently coexist. This raises questions about the dynamics of co-infections and how host life-history traits might predict co-infection versus single infection. To address these questions, we investigated the presence of two parasites, a gut parasite (Isospora coccidians) and a blood parasite (Plasmodium spp.), in House Finches (Haemorhous mexicanus), a common passerine bird in North America. We then correlated these parasitic infections with various health and condition metrics, including hematological parameters, plasma carotenoids, lipid-soluble vitamins, blood glucose concentration, body condition, and prior disease history. Our study, based on 48 birds captured in Tempe, Arizona, US, in October 2021, revealed that co-infected birds exhibited elevated circulating lutein levels and a higher heterophil:lymphocyte ratio (H/L ratio) compared to those solely infected with coccidia Isospora spp. This suggests that co-infected birds experience heightened stress and may use lutein to bolster immunity against both pathogens, and that there are potentially toxic effects of lutein in co-infected birds compared to those infected solely with coccidia Isospora sp. Our findings underscore the synergistic impact of coparasitism, emphasizing the need for more co-infection studies to enhance our understanding of disease dynamics in nature, as well as its implications for wildlife health and conservation efforts.

Japanese Encephalitis Virus and Schizophyllum commune Co-Infection in a Harbor Seal in Japan

The Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, has a wide host range, extending from pigs and ardeid birds to opportunistic dead-end hosts, such as humans and horses. However, JEV encephalitis infections in aquatic mammals are rare, with only two cases in seals reported to date. Here, we report a lethal case of JEV and Schizophyllum commune co-infection in an aquarium-housed harbor seal in Japan. We isolated JEV from the brain of the dead seal and characterized its phylogeny and pathogenicity in mice. The virus isolate from the seal was classified as genotype GIb, which aligns with recent Japanese human and mosquito isolates as well as other seal viruses detected in China and Korea, and does not exhibit a unique sequence trait distinct from that of human and mosquito strains. We demonstrated that the seal isolate is pathogenic to mice and causes neuronal symptoms. These data suggest that seals should be considered a susceptible dead-end host for circulating JEV in natural settings.

Online poetry writing at school – comparing lower secondary students’ experiences between individual and collaborative poetry writing

IntroductionThis study investigates how seventh-grade students experience online collaborative writing, its support in writing poems, and how collaboratively and individually written poems differ.MethodsThe educational design research method was used in this mixed-methods study, which was conducted in natural classroom settings to investigate students’ individual and collaborative poetry writing.ResultsThe quantitative analysis of questionnaires and qualitative thematic analysis of postexperimental interviews show that the students enjoyed collaborative writing more and found it more accessible than individual writing. They experienced that it supported them in writing better poems and increased their writing confidence. They also appreciated the support of teamwork, although individual writing gave them more liberty to explore various aspects of poetry and express their feelings.DiscussionFrom a pedagogical point of view, the students need to be provided with opportunities for collaborative poetry writing to make the writing process easier and more enjoyable. Online collaborative writing supports the process of poetry writing.

Physical Activity in Natural Settings: An Opportunity for Lifestyle Medicine

Physical activity is a well-known behavior for promoting health and preventing a variety of chronic diseases. Despite widespread knowledge of the benefits of physical activity, most Americans do not engage in sufficient physical activity. Over the past decade, there has been increasing recognition of the health benefits of spending time in nature, mediated in part through physical activity. This has led to new partnerships across health, parks and recreation, public lands, and environmental organizations to increase time spent, and physical activity, in natural settings. This review assesses the current evidence around physical activity in natural settings (PANS), strategies for promoting PANS including health professional engagement, and current gaps in the research literature.

Adipose and skin distribution of African trypanosomes in natural animal infections

BackgroundAnimal African trypanosomiasis, which is caused by different species of African trypanosomes, is a deadly disease in livestock. Although African trypanosomes are often described as blood-borne parasites, there have been recent reappraisals of the ability of these parasites to reside in a wide range of tissues. However, the majority of those studies were conducted on non-natural hosts infected with only one species of trypanosome, and it is unclear whether a similar phenomenon occurs during natural animal infections, where multiple species of these parasites may be present.MethodsThe infective trypanosome species in the blood and other tissues (adipose and skin) of a natural host (cows, goats and sheep) were determined using a polymerase chain reaction-based diagnostic.ResultsThe animals were found to harbour multiple species of trypanosomes. Different patterns of distribution were observed within the host tissues; for instance, in some animals, the blood was positive for the DNA of one species of trypanosome and the skin and adipose were positive for the DNA of another species. Moreover, the rate of detection of trypanosome DNA was highest for skin adipose and lowest for the blood.ConclusionsThe findings reported here emphasise the complexity of trypanosome infections in a natural setting, and may indicate different tissue tropisms between the different parasite species. The results also highlight the need to include adipose and skin tissues in future diagnostic and treatment strategies.Graphical

Examining Fraud Schemes In Village Financial Management

The purpose of this study was to determine the fraud scheme that occurred in Pelangi Village from planning to accountability. This research method uses a qualitative approach. This research method is often called Naturalistic where the journey occurs naturally or in natural settings, as it is, and in normal situations, there is no manipulation of events, circumstances, and conditions so that it really matches the existing reality. The results of this study indicate that the Fraud Scheme in village financial management is indeed diverse. Fraud schemes in Pelangi Village are structured from the planning stage to the accountability stage. This organized and massive fraud scheme is not only carried out by one person but by a team or a group.

Needle bacterial community structure across the species range of limber pine

Abstract Bacteria on and inside leaves can influence forest tree health and resilience. The distribution and limits of a tree species’ range can be influenced by various factors, with biological interactions among the most significant. We investigated the processes shaping the bacterial needle community across the species distribution of limber pine, a widespread Western conifer inhabiting a range of extreme habitats. We tested four hypotheses: 1) Needle community structure varies across sites, with site-specific factors more important to microbial assembly than host species selection; 2) dispersal limitation structures foliar communities across the range of limber pine; 3) the relative significance of dispersal and selection differs across sites in the tree species range, and 4) needle age structures bacterial communities. We characterized needle communities from the needle surface and tissue of limber pine and co-occurring conifers across 16 sites in the limber pine distribution. Our findings confirmed that site characteristics shape the assembly of bacterial communities across the host species range and showed that these patterns are not driven by dispersal limitation. Furthermore, the strength of selection by the host varied by site, possibly due to differences in available microbes. Our study, by focusing on trees in their natural setting, reveals real needle bacterial dynamics in forests, which is key to understanding the balance between stochastic and deterministic processes in shaping forest tree-microbe interactions. Such understanding will be necessary to predict or manipulate these interactions to support forest ecosystem productivity or assist plant migration and adaptation in the face of global change.

The Impact of Adding Dioxane Derivatives to Polyurethane Structures on their Performance and Degradation in the Environment

AbstractThe novel dioxane-polyether polyurethanes underwent a 12-month outdoor soil burial test to look into how they would degrade in a natural setting. The structure, thermal properties, surface features, and mechanical strength of the polyurethane films were compared. The initial chemical structure and subsequent chemical alterations were identified using FTIR spectroscopy. The polyurethane samples were less thermally stable throughout the duration of the soil burial test, according to TG/DTG curves. According to all findings, polyurethanes containing 1,3-dioxane-5,5-dimethanol exhibit excellent physical characteristics and mild degradation levels after being buried in soil for a year. These polyether urethanes can break down if the rigid domain structure is exposed to moisture and if microorganisms can spread into the polymer matrix. The physical properties, surface features, and degradation of polyether polyurethanes can be improved by varying the molar ratios of the hard segment components and the dioxane derivative structures.

The elimination of two restriction enzyme genes allows for electroporation-based transformation and CRISPR-Cas9-based base editing in the non-competent Gram-negative bacterium Acinetobacter sp. Tol 5

ABSTRACT Environmental isolates are promising candidates for new chassis of synthetic biology because of their inherent capabilities, which include efficiently converting a wide range of substrates into valuable products and resilience to environmental stresses; however, many remain genetically intractable and unamenable to established genetic tools tailored for model bacteria. Acinetobacter sp. Tol 5, an environmentally isolated Gram-negative bacterium, possesses intriguing properties for use in synthetic biology applications. Despite the previous development of genetic tools for the engineering of strain Tol 5, its genetic manipulation has been hindered by low transformation efficiency via electroporation, rendering the process laborious and time-consuming. This study demonstrated the genetic refinement of the Tol 5 strain, achieving efficient transformation via electroporation. We deleted two genes encoding type I and type III restriction enzymes. The resulting mutant strain not only exhibited marked efficiency of electrotransformation but also proved receptive to both in vitro and in vivo DNA assembly technologies, thereby facilitating the construction of recombinant DNA without reliance on intermediate Escherichia coli constructs. In addition, we successfully adapted a CRISPR-Cas9-based base-editing platform developed for other Acinetobacter species. Our findings provide genetic modification strategies that allow for the domestication of environmentally isolated bacteria, streamlining their utilization in synthetic biology applications. IMPORTANCE Recent synthetic biology has sought diverse bacterial chassis from environmental sources to circumvent the limitations of laboratory Escherichia coli strains for industrial and environmental applications. One of the critical barriers in cell engineering of bacterial chassis is their inherent resistance to recombinant DNA, propagated either in vitro or within E. coli cells. Environmental bacteria have evolved defense mechanisms against foreign DNA as a response to the constant threat of phage infection. The ubiquity of phages in natural settings accounts for the genetic intractability of environmental isolates. The significance of our research is in demonstrating genetic modification strategies for the cell engineering of such genetically intractable bacteria. This research marks a pivotal step in the domestication of environmentally isolated bacteria, promising candidates for emerging synthetic biology chassis. Our work thus significantly contributes to advancing their applications across industrial, environmental, and biomedical fields.

ESG performance on the value of family firms: international evidence during Covid-19

The link between the financial success of family companies during COVID-19 and their environmental, social, and governance (ESG) performance is examined for the first time in this research. We have a natural setting in the COVID-19 era to see if the market rewards family-run firms that integrate social and environmental concerns into their goals during uncertain times. Since they can enhance their image and reputation, which the market values, these companies are likely to pursue broader social objectives, such as environmental improvement (socioemotional wealth perspective); alternatively, managers can act as stewards of the family’s interests by using these initiatives to increase the company’s value (stewardship perspective). However, it is also possible that in this type of companies economic interests prevail over social wellness (“amoral familism”). Therefore, family-owned firms could be reluctant to implement ESG practices unless they yield certain socioemotional benefits, including enhancing or maintaining their reputation in the public eye. In light of the above, we use an international display of the 500 biggest family firms in the world from 2015 to 2021. Taking into account an endogenous relationship between ESG performance and family business value, the study uses generalized moments to construct a dynamic panel (GMM). The primary conclusion is that there is a positive correlation between corporate valuation and ESG performance. Nonetheless, it has been noted that the performance of the companies is negatively impacted during the COVID-19 period. However, for firms with superior ESG performance, this negative impact did not exist over this period, supporting the idea that investors view better ESG performance as a prediction of future good stock performance. The results have a variety of implications. To begin with, this study adds to the body of knowledge on the environmentally friendly and sustainable expansion of family companies by providing recommendations for investors and businesses to better understand the influence of ESG on the profitability of family businesses. Furthermore, managers have to concentrate on enhancing the ESG performance of their organizations as it has the potential to increase value, draw in investments, encourage sustainability, control risks, affect earnings, and interact with the ownership structure. Additionally, managers need to consider how important it is to have a strong ESG performance in order to mitigate the negative effects of external crises like the COVID-19 epidemic. It’s crucial to remember that the precise impact might change based on the sector and other aspects unique to each company.

Exploring autonomous and controlled motivations for nature contact to maximise health benefits

Abstract Increasing evidence indicates contact with nature supports mental, physical and social health. However, beyond a widely reported number of barriers to nature contact, the constellation of motivations for human contact with nature is under‐theorised and under‐studied. We begin to develop indicators of autonomous and controlled motivations for nature contact informed by self‐determination theory. These include intrinsic motivation (i.e. enjoyment), integrated regulation (alignment with identify and life goals), identified regulation (a means to an end), introjected regulation (emotional reasons like guilt avoidance) and external regulation (such as peer pressure). We compare these motivation indices in a nationally representative sample of 5082 adults in Australia in 2022 with the Nature Relatedness Scale (NR6), and also test associations between them and five outcomes: time spent in nature, smartphone use in nature, interest in nature prescriptions, physical activity and self‐rated health. Statistical analyses were adjusted for potential confounding. Results demonstrate people have complex mixtures of motivations with varying potency for visiting natural settings and the extent to which those motives are autonomous or controlled matters for what they do, and the benefits accrued. For example, our analyses show that more direct considerations of intrinsic, integrated and identified forms of autonomous motivation have superior explanatory power than the NR6 for time spent in nature, interest in nature prescriptions, adherence to physical activity recommendations and self‐rated health. External regulations emphasising peer approval were associated not only with no additional time in nature but also with more distractive activities when in natural environments, as defined by more smartphone and social media use while there. While introjected regulations emphasising guilt avoidance were associated with increased nature contact, they were similarly associated with time spent on smartphones and social media when in natural environments, which has been shown to undermine restoration. Synthesis and applications: We need to formally measure autonomous and controlled motivations for nature contact to better understand both why some people visit natural environments, and whether they are mindfully maximising the health benefits of those experiences. This will help to inform robust nature‐based interventions that are acceptable, effective and sustainable for everyone. Read the free Plain Language Summary for this article on the Journal blog.

Chronic nutritional deficit resulting in multiorgan disease presentations in remote Pakistan: case reviews from natural disaster settings

The aftermath of monsoon rains that fell in Pakistan from June to November 2022 were catastrophic as millions of people were affected, water systems were damaged, and agricultural production was affected. The floods aggravated the already existing nutrition crisis which largely increased children’s vulnerability. Disaster Medicine and Dentistry (DMD), a UK-registered consultancy organization, provided support to the affected households in Dera Ismail Khan through the provision of water pumps, food rations, and climate-resistant houses. It also established a mobile medical unit that provided medical services to affected households. All the children that came for consultation were far below the expected height or weight range for their age, thus showing signs of chronic malnutrition. They also presented with multiorgan diseases as a result of the chronic malnutrition, and DMD’s onsite and remote consultants managed them all. We took a wholistic approach to managing the nutrition crisis and the floods, which significantly improved the health outcomes of the affected individuals and households.

The Privacy Price of School Safety Stakeholders' Perceptions Towards the Use of Closed-Circuit Television (CCTV) in Schools

CCTV surveillance cameras have played an influential role in safeguarding security, discipline, and a conducive learning environment in Nepali schools. However, as surveillance technology is gradually being installed and used, there is a growing concern about privacy violations. In this context, the article aims to explore the distinct perceptions and understanding of the key stakeholders, namely students, teachers and administrators, regarding using CCTV surveillance technology in their natural setting. This article especially seeks the stakeholders’ perceptions on the impact of surveillance technology on students and teachers about their privacy rights. To meet the objective, I have employed a qualitative study that follows the interpretivists’ research paradigm. I have purposively selected five respondents from each school, incorporating three students and two teaching faculties cum administrative persons. I employed semi-structured interviews at three secondary schools from inner Terai of Nawalpur district. Moreover, I utilized non-participant observation, field notes and google-form as a tool to gather content-rich data and verify the information accumulated through semi-structured interviews, regarding their lived and subjective perceptions about using CCTVs. Findings indicate effectively managing and mitigating various risk factors within schools’ premises, and cultivating a culture of shared responsibilities among students and teachers for building a conducive learning culture in schools. It is also found that surveillance technology in Nepali schools nurtures suspicion among stakeholders, along with acknowledging the importance of comprehensive policy, and students’ privacy concerns to ensure a safe and friendly learning culture.

Subjective intoxication predicts alcohol-related consequences at equivalent alcohol concentrations in young adults using ecological momentary assessment and alcohol sensors.

Subjective intoxication (SI) when drinking may serve as an internal barometer of whether to continue drinking or engage in potentially unsafe behavior. Mobile assessments offer the potential to use SI as a prospective risk indicator during drinking episodes; little evidence exists for the validity of real-time SI measures. We test the correspondence of SI with estimated blood alcohol concentration and transdermal alcohol concentration (TAC) in young adults' natural settings. We provide a novel test of whether SI features (peak and mean SI) uniquely predict consequences adjusting for alcohol concentration. Two hundred twenty-two heavy-drinking young adults (Mage = 22.3, 64% female, 79% non-Hispanic White, 84% undergraduates) participated in a 6-day study that used ecological momentary assessment of drinking and TAC sensors. SI was assessed every 30 min during drinking episodes. Multilevel modeling was used to test hypotheses. Momentary SI and estimated blood alcohol concentration had moderate associations at the moment and day levels (standardized βs = 0.5-0.6); SI was moderately associated with TAC at the day level (βs = 0.5). Associations between SI and alcohol concentration varied widely between persons and across days. Day-level SI features predicted consequences when adjusting for alcohol concentration (incidence rate ratios, IRRs = 1.29-1.70). Our two-item SI measure shows evidence of validity in real-world settings with heavy-drinking young adults. SI was significantly correlated with alcohol concentration and was a unique predictor of consequences. The strength of these associations varied greatly across persons and days. Real-time SI measurement may be useful in preventive interventions, but continued research is needed into when and for whom momentary SI is most predictive of risk. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Tectono-metamorphic evolution of the Central Bundelkhand Craton, India from geochemistry and bulk composition modelling of amphibolite enclaves

The Bundelkhand Craton (BuC) represents a significant Archean terrane within the northern Indian Craton and yet its tectono-metamorphic evolutionary history remains relatively understudied. Our investigation involves detailed petrography, geochemistry, and bulk composition modeling of both garnet-bearing and garnet-absent amphibolites with a two-fold objective: (i) to constrain the protolithic nature and tectonic settings involved in the genesis of these rocks, and (ii) to propose a tectono-metamorphic evolutionary history for the BuC. The BuC amphibolites originate from basalt and andesitic-basalt protoliths. Their trace element compositions reveal negative anomalies in Nb, Ta, and Ti, while their rare earth element (REE) normalized patterns indicate enrichment in light REEs over heavy REEs. The basaltic protolith is interpreted to have formed during orogeny in a compressional tectonic regime at the active margins of island arcs in a subduction-related setting. This interpretation is supported by various discrimination plots for amphibolites, such as Nb/Th vs Zr/Nb, Zr vs Zr/Y, and Th/Nb vs Ce/Nb, as well as high Th/Yb and low Nb/Yb contents— all of which suggest an island arc setting influenced by subduction. These amphibolites have undergone three distinct phases of metamorphism, as evidenced by petrography, mineral chemistry, and bulk composition modeling. This interpretation is further supported by geochemical discrimination diagrams which indicate that a subduction tectonic setting was active during peak metamorphism. During the pre-peak phase, the garnet-bearing amphibolites experienced pressure and temperature conditions ranging from 6.25 to 6.5 kbar and 580 to 590ºC, while the garnet-absent amphibolites underwent conditions from 5.0 to 5.8 kbar and 400 to 450ºC. Peak metamorphism was observed at pressures ranging from 6.8 to 7.4 kbar and temperatures from 760 to 805ºC for the garnet-bearing amphibolites, and at pressures from 7.0 to 7.4 kbar and temperatures from 785 to 810ºC for the garnet-absent amphibolites. The metamorphic retrograde conditions for the garnet-bearing amphibolites are defined by P-T conditions ranging from 4.45 to 4.75 kbar and 585 to 615ºC, while for the garnet-absent amphibolites, it ranges from 3.1 to 4.0 kbar and 620 to 710ºC. The mineral assemblages and P-T conditions delineate a clockwise P-T path for both, garnet-bearing and garnet-absent amphibolites from the Babina and Mauranipur regions. This suggests that the rocks underwent a burial process amid subduction tectonic settings in an arc-related environment, followed by a decompression stage that brought them to the surface.

Methane emissions from residential natural gas meter set assemblies

Residential natural gas meter set assemblies (MSAs) emit methane (CH4), but reported emissions factors vary. To test existing emissions factors, we quantified CH4 emissions from 37 residential MSAs in Calgary, Alberta, Canada. A notable difference with previous studies is the targeted measurement of regulator vents in this study, which were measured with a static chamber, while fugitives were measured with a modified hi-flow sampler. Emissions were dominated by pressure regulator vents (emissions factor = 1.18 g CH4/h/MSA), but 7 fugitives were found (emissions factor = 0.018 g CH4/h/MSA). Six regulator vents were emitting at notably higher rates (≥ 1.79 g CH4/h/MSA). The total empirical emissions factor was 1.20 g CH4/h/MSA (95 % CI, 1.03 to 1.37 g/h/MSA). This is ∼7 times higher than the emissions factor for residential MSAs used in the U.S. EPA's Greenhouse Gas Inventory, which may not include emissions from regulator vents. Upscaling to annual CH4 emissions in Calgary indicates 3234.6 t CH4/yr (95 % CI, 2776.4 t to 3692.9 t CH4/yr) could be emitted from MSAs. This is equivalent to 4.1 % (95 % CI, 3.5 % to 4.7 %) of total city-level CH4 emissions as estimated with satellite data. Results suggest residential MSA emissions may be under-estimated and further study isolating root causes of regulator vent emissions is required to guide mitigation and improve emissions modeling.

Visual Mind: Visual Question Answering (VQA) with CLIP Model

Abstract: This paper proposes a Visual Question Answering (VQA) problem using CLIP models. The proposed approach suggests an enhanced VQA-CLIP model with additional layers for better computational performance. VQA is an increasingly important task that aims to answer open-ended questions based on images. This task has numerous applications in various fields such as medicine, education, and surveillance. The VizWiz dataset, specifically designed to assist visually impaired individuals, consists of image/question pairs along with 10 answers per question, recorded by blind participants in a natural setting. The task involves predicting answers to questions and determining when a question is unanswerable. In this study, we will utilize the VizWiz dataset and employ the CLIP model with an additional linear layer, a multimodal, zero-shot model known for its efficiency in processing image and text data. By leveraging the unique capabilities of CLIP, and benchmarked against state-ofthe-art approaches. Results indicate a competitive or better performance of the VQA model.

Public perceptions of climate change during the COVID-19 crisis: Evidence from social media data in China

An emerging body of literature highlights the impact of crises and disasters in shaping climate change attitudes, yet the findings have been equivocal. The outbreak and spread of COVID-19 have posed great threats worldwide, but it also provides opportunities to explore the impact of crises in natural settings. Based on social media data in the early stage of the emergence of the pandemic in China, this study addresses the controversy and examines the extent to which the outburst of COVID-19 influences climate change attitudes. Frequency and popularity analyses of the posts suggest that public attention shifted toward the devastating impacts of the epidemic, whereas sentiment analyses indicate that the posts contain a more positive sentiment as people are more proactive in taking action to address climate change after the coronavirus outbreak. This study provides us with a more nuanced understanding of the underlying influencing factors of climate change attitudes and evaluates the implications of the pandemic and other uncertain events on environmental concerns.

Interactive transcriptome analyses of Northern Wild Rice (Zizania palustris L.) and Bipolaris oryzae show convoluted communications during the early stages of fungal brown spot development.

Fungal diseases, caused mainly by Bipolaris spp., are past and current threats to Northern Wild Rice (NWR) grain production and germplasm preservation in both natural and cultivated settings. Genetic resistance against the pathogen is scarce. Toward expanding our understanding of the global gene communications of NWR and Bipolaris oryzae interaction, we designed an RNA sequencing study encompassing the first 12 h and 48 h of their encounter. NWR activated numerous plant recognition receptors after pathogen infection, followed by active transcriptional reprogramming of signaling mechanisms driven by Ca2+ and its sensors, mitogen-activated protein kinase cascades, activation of an oxidative burst, and phytohormone signaling-bound mechanisms. Several transcription factors associated with plant defense were found to be expressed. Importantly, evidence of diterpenoid phytoalexins, especially phytocassane biosynthesis, among expression of other defense genes was found. In B. oryzae, predicted genes associated with pathogenicity including secreted effectors that could target plant defense mechanisms were expressed. This study uncovered the early molecular communication between the NWR-B. oryzae pathosystem, which could guide selection for allele-specific genes to boost NWR defenses, and overall aid in the development of more efficient selection methods in NWR breeding through the use of the most virulent fungal isolates.

Tactile Landmarks: the Relative Landmark Location Alters Spatial Distortions

Abstract The influence of landmarks, that is, nearby non-target stimuli, on spatial perception has been shown in multiple ways. These include altered target localization variability near landmarks and systematic spatial distortions of target localizations. Previous studies have mostly been conducted in the visual modality using temporary, artificial landmarks or the tactile modality with persistent landmarks on the body. Thus, it is unclear whether both landmark types produce the same spatial distortions as they were never investigated in the same modality. Addressing this, we used a novel tactile setup to present temporary, artificial landmarks on the forearm and systematically manipulated their location to either be close to a persistent landmark (wrist or elbow) or in between both persistent landmarks at the middle of the forearm. Initial data (Exp. 1 and Exp. 2) suggested systematic differences of temporary landmarks based on their distance from the persistent landmark, possibly indicating different distortions of temporary and persistent landmarks. Subsequent control studies (Exp. 3 and Exp. 4) showed this effect was driven by the relative landmark location within the target distribution. Specifically, landmarks in the middle of the target distribution led to systematic distortions of target localizations toward the landmark, whereas landmarks at the side led to distortions away from the landmark for nearby targets, and toward the landmark with wider distances. Our results indicate that experimental results with temporary landmarks can be generalized to more natural settings with persistent landmarks, and further reveal that the relative landmark location leads to different effects of the pattern of spatial distortions.