Natural Settings Research Articles

Reductive Dissolution of NCM Cathode through Anaerobic Respiration by Shewanella putrefaciens.

The consumption of lithium-ion batteries (LIBs) has considerably increased over the past decade, leading to a rapid increase in the number of spent LIBs. Exposing spent LIBs to the environment can cause serious environmental harm; however, there is a lack of experimentally obtained information regarding the environmental impacts of abandoned cathode materials. Here, we report the interactions between Shewanella putrefaciens, a microorganism commonly found in diverse low-oxygen natural settings, and LiNi0.6Co0.2Mn0.2O2 (NCM622) under anaerobic conditions. We present compelling evidence that the anaerobic respiration of Shewanella putrefaciens triggers ∼59 and ∼78% dissolution of 0.2 g/L pristine and spent NCM622, respectively. We observed that Shewanella putrefaciens interacted with the pristine and the spent NCM622 under anaerobic conditions at a neutral pH and room temperature and induced the reduction of Ni, Co, and Mn, resulting in the subsequent dissolution of Li, Ni, Co, and Mn. Moreover, we found that secondary mineralization occurred on the surface of reacted NCM622. These findings not only shed light on the substantial impact of microbial respiration on the fate of discarded cathode materials in anaerobic environments but also reveal the potential for sustainable bioleaching of cathodes in spent LIBs.

Critical appraisal of the chorioallantoic membrane model for studying angiogenesis in preclinical research.

Angiogenesis, the biological mechanism by which new blood vessels are generated from existing ones, plays a vital role in growth and development. Effective preclinical screening is necessary for the development of medications that may enhance or inhibit angiogenesis in the setting of different disorders. Traditional in vitro and, in vivo models of angiogenesis are laborious and time-consuming, necessitating advanced infrastructure for embryo culture. A challenge encountered by researchers studying angiogenesis is the lack of appropriate techniques to evaluate the impact of regulators on the angiogenic response. An ideal test should possess reliability, technical simplicity, easy quantifiability, and, most importantly, physiological relevance. The CAM model, leveraging the extraembryonic membrane of the chicken embryo, offers a unique combination of accessibility, low cost, and rapid development, making it an attractive option for angiogenesis assays. This review evaluates the strengths and limitations of the CAM model in the context of its anatomical and physiological properties, and its relevance to human pathophysiological conditions. Its abundant capillary network makes it a common choice for studying angiogenesis. The CAM assay serves as a substitute for animal models and offers a natural setting for developing blood vessels and the many elements involved in the intricate interaction with the host. Despite its advantages, the CAM model's limitations are notable. These include species-specific responses that may not always extrapolate to humans and the ethical considerations of using avian embryos. We discuss methodological adaptations that can mitigate some of these limitations and propose future directions to enhance the translational relevance of this model. This review underscores the CAM model's valuable role in angiogenesis research and aims to guide researchers in optimizing its use for more predictive and robust preclinical studies. The highly vascularized chorioallantoic membrane (CAM) of fertilized chicken eggs is a cost-effective and easily available method for screening angiogenesis, in comparison to other animal models.

Key Technologies for Autonomous Fruit- and Vegetable-Picking Robots: A Review

With the rapid pace of urbanization, a significant number of rural laborers are migrating to cities, leading to a severe shortage of agricultural labor. Consequently, the modernization of agriculture has become a priority. Autonomous picking robots represent a crucial component of agricultural technological innovation, and their development drives progress across the entire agricultural sector. This paper reviews the current state of research on fruit- and vegetable-picking robots, focusing on key aspects such as the vision system sensors, target detection, localization, and the design of end-effectors. Commonly used target recognition algorithms, including image segmentation and deep learning-based neural networks, are introduced. The challenges of target recognition and localization in complex environments, such as those caused by branch and leaf obstruction, fruit overlap, and oscillation in natural settings, are analyzed. Additionally, the characteristics of the three main types of end-effectors—clamping, suction, and cutting—are discussed, along with an analysis of the advantages and disadvantages of each design. The limitations of current agricultural picking robots are summarized, taking into account the complexity of operation, research and development costs, as well as the efficiency and speed of picking. Finally, the paper offers a perspective on the future of picking robots, addressing aspects such as environmental adaptability, functional diversity, innovation and technological convergence, as well as policy and farm management.

New insights into honey bee viral and bacterial seasonal infection patterns using third-generation nanopore sequencing on honey bee haemolymph

Honey bees are rapidly declining, which poses a significant threat to our environment and agriculture industry. These vital insects face a disease complex believed to be caused by a combination of parasites, viruses, pesticides, and nutritional deficiencies. However, the real aetiology is still enigmatic. Due to the conventional analysis methods, we still lack complete insights into the honey bee virome and the presence of pathogenic bacteria. To fill this knowledge gap, we employed third-generation nanopore metagenomic sequencing on honey bee haemolymph to monitor the presence of pathogens over almost a year. This study provides valuable insights into the changes in bacterial and viral loads within honey bee colonies. We identified different pathogens in the honey bee haemolymph, which are not included in honey bee screenings. These pathogens comprise the Apis mellifera filamentous virus, Apis rhabdoviruses, and various bacteria such as Frischella sp. and Arsenophonus sp. Furthermore, a sharp contrast was observed between young and old bees. Our research proposes that transgenerational immune priming may play a role in shaping infection patterns in honey bees. We observed a significant increase in pathogen loads in the spring, followed by a notable decrease in pathogen presence during the summer and autumn months. However, certain pathogens seem to be able to evade this priming effect, making them particularly intriguing as potential factors contributing to mortality. In the future, we aim to expand our research on honey bee transgenerational immune priming and investigate its potential in natural settings. This knowledge will ultimately enhance honey bee health and decrease colony mortality.

Physicochemical Hydrodynamics of Particle Diffusiophoresis Driven by Chemical Gradients

Chemical gradients, the spatial variations in chemical concentrations and components, are omnipresent in environments ranging from biological and environmental systems to industrial processes. These thermodynamic forces often play a central role in driving transport processes taking place in such systems. This review focuses on diffusiophoresis, a phoretic transport phenomenon driven by chemical gradients. We begin by revisiting the fundamental physicochemical hydrodynamics governing the transport. Then we discuss diffusiophoresis arising in flow systems found in natural and artificial settings. By exploring various scenarios where chemical gradients are encountered and exploited, we aim to demonstrate the significance of diffusiophoresis and its state-of-the-art development in technological applications.

Roots and Refuge: A Critical Exploration of Nature in Black Visual Narratives

This article examined the underrepresentation of Black characters in children’s picture books, particularly in natural settings, and its effect on Black children’s relationship with nature. Through an analysis of four contemporary picture books, the study revealed how visual depictions challenge these exclusions and expand narratives about Black engagement with the natural world. Utilizing visual semiotics and the theory of Black Aliveness, this research underscores the transformative power of illustrations by Black artists in enriching children’s literature and advancing joy.

Translanguaging for the construction of instructional immediacy in a Mandarin–Japanese crosslinguistic class

Abstract Accumulating studies on teachers’ instructional immediacy consistently show a strong positive correlation with teaching effectiveness. To expand the research in a bilingual and cross-cultural context, the current study investigates the construction of instructional immediacy in a natural classroom setting by theoretically anchoring on translanguaging, which refers to the idea of promoting the integration of all linguistic and nonlinguistic resources to achieve effective pedagogy with multilingual speakers. We quantified the nonverbal facial expressions to compare the teacher’s endeavours in different language modes when constructing instructional immediacy, and we used Multimodal Conversation Analysis (MCA) to examine his endeavours. The results revealed language-specific facial expressions which are attributed to varied teaching purposes. We conclude that translanguaging can serve as an effective tool for fostering instructional immediacy in multilingual classrooms.

Commentary: Environmental RNA and the assessment of organismal function in the field

Environmental RNA (eRNA) is an emerging technique with significant potential for the assessment of organismal function in field settings. It has the advantage of being non-invasive, facilitating insight into the physiological status of an organism without complications associated with processes such as capture, handling, and transportation from the field to the laboratory. It is hypothesised that eRNA approaches will be especially valuable for assessing sublethal stress of species living in environmental settings undergoing change and could therefore be integral for examining population health and for testing hypotheses regarding organismal physiology developed from laboratory studies. However, the successful application of eRNA approaches requires further data regarding the stability and persistence of eRNA in natural substrates; established and validated relationships between molecular biomarkers and the physiological processes they participate in; and an understanding of the contributions of different epithelia in direct contact with the environment (skin, gill, gut) to the eRNA transcriptome. The utility of microRNA as a component of the eRNA pool should be an area of specific future research focus. Ultimately, eRNA has the potential to provide fundamental physiological information regarding the responses of organisms in their natural settings and could increase the sensitivity and acuity of biomonitoring efforts.

20th-Century World Built Heritage Facing Water: Conservation of Fallingwater and Boa Nova Tea House

The conservation of 20th-century architectural heritage presents unique challenges due to the modern materials and construction techniques used by pioneering architects. This paper investigates the preservation challenges of iconic architectural works exposed to water environments, focusing on Fallingwater by Frank Lloyd Wright and the Boa Nova Tea House by Álvaro Siza Vieira. These structures, situated in natural settings with significant exposure to water, serve as case studies for examining conservation methods and their broader implications. This study explores archival documents, interviews with conservation experts, and literature reviews to highlight the main degradation phenomena related to the impact of environmental conditions on the material’s integrity. Despite limited specific data on monitoring and maintenance, this research underscores the importance of ongoing conservation efforts. Fallingwater’s case highlights the necessity of restoration techniques, while the Boa Nova Tea House illustrates minimal intervention strategies to maintain authenticity. This study concludes that the effective conservation of 20th-century built heritage necessitates a balance between preserving original design intents and adapting to environmental challenges. It emphasizes the need for innovative conservation strategies, adherence to international guidelines, and the establishment of comprehensive management and monitoring plans to safeguard the integrity and authenticity of culturally significant architecture.

Invasion stage and competition intensity co-drive reproductive strategies of native and invasive saltmarsh plants: Evidence from field data

Biological invasion poses a significant threat to biodiversity conservation and also results in substantial economic loss including the excessive cost of management to control it. Still, its impact on plant sexual reproduction strategies remains underexplored in natural settings. We conducted a field experiment on native Phragmites australis and invasive Spartina alterniflora in Bohai Bay and assessed plant size (aboveground biomass and height) and sexual reproduction (ear biomass, reproductive allocation, etc.) in conjunction with water and soil properties. The results showed that during the early stage of invasion, the two species declined in size and sexual reproduction, with S. alterniflora showing a lesser decline than P. australis. However, in the late stage of invasion, S. alterniflora maintained its plant size by reducing its investment in sexual reproduction. Moreover, significant reproductive allometries were demonstrated by S. alterniflora under different competition intensities. P. australis displayed heightened sensitivity to water properties and soil non-resource conditions, while S. alterniflora adapted its inherent traits and environmental tolerance. S. alterniflora allocated more resources to thriving as an individual, while P. australis prioritized reproduction by increasing seed production. Overall, this study revealed the reproductive strategies that invasive and native species employ in response to competition and environmental factors, thereby offering crucial insights for conservation and management efforts.

Autonomous Planetary Liquid Sampler (APLS) for In Situ Sample Acquisition and Handling from Liquid Environments.

Many natural and artificial liquid environments, such as rivers, oceans, lakes, water storage tanks, aquariums, and urban water distribution systems, are difficult to access. As a result, technology is needed to enable autonomous liquid sampling to monitor water quality and ecosystems. Existing in situ sample acquisition and handling systems for liquid environments are currently limited to a single use and are semi-autonomous, relying on an operator. Liquid sampling systems should be robust and light and withstand long-term operation in remote locations. The system components involved in liquid sampling should be sterilisable to ensure reusability. Here, we introduce a prototype of a liquid sampler that can be used in various liquid environments and may be valuable for the scientific characterisation of different natural, remote, and planetary settings. The Autonomous Planetary Liquid Sampler (APLS) is equipped with pre-programmed, fully autonomous extraction, cleaning, and sterilisation functionalities. It can operate in temperatures between -10 °C and 60 °C and pressure of up to 0.24 MPa (~24 m depth below mean sea level on Earth). As part of the control experiment, we demonstrate its safe and robust autonomous operation in a laboratory environment using a liquid media with Bacillus subtilis. A typical sampling procedure required 28 s to extract 250 mL of liquid, 5 s to fill the MilliQ water, 25 s for circulation within the system for cleaning and disposal, and 200 s to raise the system temperature from ~30 °C ambient laboratory temperature to 150 °C. The temperature is then maintained for another 3.2 h to sterilise the critical parts, allowing a setup reset for a new experiment. In the future, the liquid sampler will be combined with various existing analytical instruments to characterise the liquid solution and enable the autonomous, systematic monitoring of liquid environments on Earth.

Incorporating Technology in the Wilderness

This case examines a challenge faced by outdoor-based accommodations and attractions regarding guest service technological expectations through the lens of copresence and technology dependence. Given the remote nature of these types of locations and lack of viable infrastructure, it can be difficult for guests and employees alike to work and play without technology such as Wi-Fi or cellular access. Additionally, the case shows how upper management and governmental entities in charge of national park tourism attempt to address the inclusion of technology access within a natural setting. The case discusses various solutions—such as utilizing a paid internet service at multiple lodges within the park or “disguised” cellular towers. However, determining the appropriate solution can be difficult, as management and other stakeholders need to balance technological guest satisfaction with preserving the natural space of the park and the wilderness that attract guests to the destination. As seen within the case, guests respond both positively and negatively to the lack of technological access within the park, which results in a challenging situation for park management to resolve.

Boys’ Underperformance, Teacher Scoring Bias and Unbalanced Teacher Market in Rural Philippines

This study exploits a natural experimental setting in rural Philippines to investigate the reversed gender disparity in education. We compare two rating systems implemented in the Philippines, observing that boys’ underperformance persists in evaluations by teachers who know the examinees. Conversely, this bias is not evident in external assessments where the examinees’ attributes are concealed from evaluators. Our empirical analyses identify two underlying channels. The first is the disparity rooted in a stereotype against male students. The second is teacher-rated scores capturing a broader range of diligence aspects, such as attendance, where male students are likely to underperform. This bias is also amplified by the likelihood of student–teacher gender (mis)match, reflecting teacher labour-market conditions in developing countries. This study has limitations due to the relatively small sample to generalise and determine which mechanism prevails over the other. Nonetheless, we emphasise that gender disparity is driven by stereotypes (i.e. being male or diligent towards a specific gender) of one gender over the other.

Wheat leaf localization and segmentation for yellow rust disease detection in complex natural backgrounds

Wheat yellow rust disease poses a significant threat to global wheat yield and grain quality. Early detection of this disease will help to minimize the loss caused by its effects. Existing models work well on images taken in a controlled environment, whereas a uniform background is placed behind the leaf, but these models fail to produce good results in natural settings. Previous research also involves manual interventions in the pipeline to achieve good classification results such as cropping the images, using uniform backgrounds, etc. These systems are not practical to use in natural environments where there will be a lot of background noise to the image and manual cropping becomes an extra step for the farmer. Moreover, the unavailability of the dataset in which images of leaves are taken in a natural setting became another challenge. In this research, a dataset is curated and leaves are annotated for object detection, object segmentation further the leaves are classified into 3 classes ie healthy, resistant, and susceptible. A novel unsupervised image rotation algorithm is proposed that takes input from YOLOv8 to align the leave in such a way that maximum background can be removed by a rectangular bounding box . Then the comparison between multiple state-of-the-art segmentation models ie. UNET, Segment-Anything (SAM), Segnet, LinkNet, PSPNet, FPN, Deep-Labv3+ (Xception), and DeepLabv3+ (Mo-bileNet) has shown that UNET has outperformed all the other segmentation models with an IOU score of 0.9563. Lastly for classification, the performance of multiple convolution neural networks ie. VGG16, Resnet 101(v2), Xception, Mo-bileNetV2, and Transformer-based models ie. Swin trans-former and MobileVit have been compared. Swin transformer has outperformed the state-of-the-art CNN models with an accuracy of 95.8%. This paper proposes a complete robust pipeline that can be deployed in natural environment and does not need any manual intervention to produce good results. This research shows that good localization of leaves and removal of unwanted background noise at the earliest stage of the pipeline will assist the segmentation model to effectively segment the leaf from the background which will enable classification models to achieve high classification accuracy, even when dealing with very small datasets.

Analisis Produktivitas Pegawai terhadap Pelayanan Permasalahan Kinerja Aparatur Sipil Negara di Direktorat Status Kedudukan Kepegawaian Kantor Pusat Badan Kepegawaian Negara (BKN) Jakarta

This study aims to analyze the performance of human resources in the Directorate of Civil Service Status at the State Civil Service Agency in handling service problems or ASN status cases. Labor productivity is influenced by various factors related to the workforce itself as well as those related to the company environment and government policies as a whole, such as education, skills, discipline, attitude and work ethics. Research methods are classified based on the objectives and level of natural setting of the object, research methods can be classified into basic research objectives, applied research methods and research and development. In this study, the researcher chose the type of Qualitative research to present and describe in detail and in detail the analysis of employee productivity in serving ASN performance problems at the Directorate of Civil Service Status of the BKN. Research Results Overall, the quality of service at the Directorate of Civil Service Status of the State Civil Service Agency (BKN) can be categorized as very good, supported by clarity of information, ease of access through various communication channels, and employee responsiveness in handling complaints. The completeness of the files submitted by the proposing agency greatly affects the speed of the service process. When the files are complete, the process can run quickly and efficiently. However, incomplete files are often the cause of delays in service. and the use of technology, such as the ASN SI application, is very helpful in improving the efficiency and effectiveness of services. Technology allows ASN to access information and services more quickly and easily.

Bumble bees do not avoid field-realistic but innocuous concentrations of cadmium and copper.

Bee populations are facing numerous stressors globally, including environmental pollution by trace metals and metalloids. Understanding whether bees can detect and avoid these pollutants in their food is pivotal, as avoidance abilities may mitigate their exposure to xenobiotics. While these pollutants are known to induce sublethal effects in bees, such as disrupting physiological mechanisms, their potential impacts on locomotive abilities, fat metabolism, and reproductive physiology remain poorly understood. In this study, utilising workers of the buff-tailed bumble bee and two prevalent trace metals, namely cadmium and copper, we aimed to address these knowledge gaps for field-realistic concentrations. Our findings reveal that workers did not reject field-realistic concentrations of cadmium and copper in sucrose solutions. Moreover, they did not reject lethal concentrations of cadmium, although they rejected lethal concentrations of copper. Additionally, we observed no significant effects of field-realistic concentrations of these metals on the walking and flying activities of workers, nor on their fat metabolism and reproductive physiology. Overall, our results suggest that bumble bees may not avoid cadmium and copper at environmental concentrations, but ingestion of these metals in natural settings may not adversely affect locomotive abilities, fat metabolism, or reproductive physiology. However, given the conservative nature of our study, we still recommend future research to employ higher concentrations over longer durations to mimic conditions in heavily polluted areas (i.e., mine surrounding). Furthermore, investigations should ascertain whether field-realistic concentrations of metals exert no impact on bee larvae.

Rebuilding human-nature connections in children and adolescents: insights from a meta-analysis

Beyond correlational studies exploring the benefits of nature exposure, there have been studies assessing interventions that aim to improve nature connection in children and adolescents. A feeling of connection with nature or ‘nature connectedness’ describes our attitude towards nature, primarily reflecting the emotional, cognitive, and experiential aspects. This meta-analysis of 29 studies explored the impact of experimental manipulations on nature connection in young people (less than 18 years old). The results showed that such interventions had a significant positive effect on nature connections in children and adolescents. The presence of environmental education programmes, the quality of engagement with nature (passive or active), and the duration and frequency of intervention (one-off, repeated, or residential) were predictors of effect size. The quality of engagement, such as active participation in environmental education programmes or an intensive residential experience, may be more important than the type of natural setting. Several important implications for future research and practice are suggested by the results, including the need to understand the factors that contribute to improved nature connection, to examine different nature engagement activities, and to develop interventions with regular nature engagement.

Circadian clock period length is not consistently linked to chronotype in a wild songbird.

Circadian clock properties vary between individuals and relate to variation in entrained timing in captivity. How this variation translates into behavioural differences in natural settings, however, is poorly understood. Here, we tested in great tits whether variation in the free-running period length (tau) under constant dim light (LL) was linked to the phase angle of the entrained rhythm ("chronotype") in captivity and in the wild, as recently indicated in our study species. We also assessed links between tau and the timing of first activity onset and offset under LL relative to the last experienced light-dark (LD) cycle. We kept 66 great tits, caught in two winters, in LL for 14 days and subsequently released them with a radio transmitter back to the wild, where their activity and body temperature rhythms were tracked for 1 to 22 days. For a subset of birds, chronotype was also recorded in the lab before release. Neither wild nor lab chronotypes were related to tau. We also found no correlation between lab and wild chronotypes. However, the first onset in LL had a positive relationship with tau, but only in males. Our results demonstrate that links between tau and phase of entrainment, postulated on theoretical grounds, may not consistently hold under natural conditions, possibly due to strong masking. This calls for more holistic research on how the many components of the circadian system interact with the environment to shape timing in the wild. Wild birds showed chronotypes in the field that were unlinked to their circadian period length tau measured in captivity. In males only, the first onset of activity after exposure to constant dim light did correlate with tau. Our study emphasises the need to investigate clocks in the real world, including a need to better understand masking.

Ageing behavior of starch-based food packaging bioplastics in riparian sediments and sediment-derived dissolved organic matter in the soil environment

Riparian sediment (RS) is a translational zone separating aquatic and terrestrial ecosystems. To this date, the bioplastic's UV ageing and biodegradation features in these contaminated sediments remain unknown. It is a considerable concern to investigate whether a food packaging film can interact with RS and riparian sediment-derived Dissolved Organic Matter (RS-DOM) during biodegradation and UV ageing respectively, after disposal in a natural environmental setting. To address this research gap, for the first time, this study investigates the biodegradation and UV ageing of starch/PPst/GTR films intended for food packaging applications in RS and RS-DOM respectively. The findings revealed that RS comprises major fulvic acid DOM components. Remarkably, research demonstrates the leaching of humic acid-like DOM from the film promotes aromaticity and humification as UV ageing progresses from the third to the tenth day. Comparable DOM samples were darkly analysed, revealing aromatic proteins I and II. Furthermore, an elevated carbonyl carboxyl index confirmed significant degradation of films during UV ageing. Lesser humification, aromaticity, and higher biological activity were confirmed by a HI < 10 and BIX > 0.6 respectively. In comprehension, these findings reveal that the starch/PPst/GTR food packaging film will have a lesser adverse environmental impact after disposal, offering a hopeful outlook for the future of bioplastics.

Rediscover nearby nature: Exploring the well-being effect and influence factors of camping at different stages of COVID-19

The COVID-19 pandemic has significantly impacted people's lifestyles. Camping, as an outdoor activity, has become increasingly popular in urban China, especially during the pandemic. However, existing literature lacks the recognition of changes in the psychological aspects of camping at various stages of health crises. This study developed a comprehensive model to explore the connections between constraints, motivations, satisfaction, and subjective well-being in camping experiences at different stages of COVID-19 pandemic. A two-wave tracking survey was collected in May 2022 during the COVID-19 period, and April 2023 in the post-COVID-19 period. The findings indicate that travelers faced fewer constraints after the health crisis, with their satisfaction levels less affected by these constraints. Travel motivations, however, did not show significant changes. It is worth noting that while camping satisfaction continued to positively impact subjective well-being, its influence notably decreased after the health crisis. This research illuminates the evolving relationship between antecedents and consequences of camping throughout different stages of health crises, providing insights for enhancing camping product and experience design, as well as campground planning and management. Management implicationsThis pioneering study examines camping in nearby natural settings across different phases of COVID-19, elucidating differences in well-being outcomes and factors influencing camping experiences. Findings offers invaluable insights into current practices and management strategies related to camping. Campsite managers need to pay attention to the psychological processes of campers at different stages of the COVID-19 pandemic. Furthermore, governments and service providers can tailor products and services based on tourists' evolving needs and psychological changes.