The article identifies and characterizes two strategic factors that influenced the formation of the Department of General Biology and Methodology of Teaching Natural Sciences as a holistic open self-regulatory system. In particular, an important factor was the organizational and methodological as well as research activities of the heads of the department, I. V. Shust, A. V. Tsarenko, V. V. Hrubinko, who contributed to the formation of a new generation of scientists and lecturers, the emergence of scientific schools and the development of new areas of biological and methodological investigations. A new impetus to intensify the activities of the teaching staff in all areas was the association of the Department of General Biology with the Department of Methodology of Teaching Natural Sciences, which had a positive effect on cooperation between employees of higher pedagogical education with teachers of secondary schools and pupils. It has been defined that the development of collective scientific topics of pedagogical direction served as the basis for the development and implementation of a model of partnering relationships organization in the "Higher educational establishment – school" system, a systemically important factor of which is the implementation of the principles of child centeredness and student centeredness in parity collaboration of participants in the educational process. The model is based on various types of innovative activities regarding cooperation between schools and higher educational establishments in three promising areas: working out the mechanisms of interaction between secondary schools and higher educational establishments in order to modernize the forms and methods of organizing the educational process; creating conditions for professional self-determination of pupils and increasing the level of competence of future university entrants; creation by higher education establishments of an educational environment for teachers to implement lifelong learning.
 In the process of implementing the model, a number of modern forms of interaction between educational institutions have been tested. In particular, preparation and approbation of textbooks, teaching and methodical manuals for pupils and teachers, carrying out of joint master classes, trainings, workshops, scientific and methodical seminars, conferences, forums, etc.; organization of career guidance events – Doors Open Days, festival of professions "Hospitality in TNPU", All-Ukrainian career guidance Olympiad in Biology, functioning of the Summer Chemical and Biological School of TNPU "My future professional choice"; development and implementation of educational programs of postgraduate education for advanced training and professional development of teachers of biology and natural sciences .