Natural Sciences Research Articles

An Overview of the Basic Theory of Probability Theory and its Commercial Application

Abstract. Probability theory is a key field of mathematics that focus on developing theoretical models and mathematical frameworks to describe and analyze random events or processes. As a part of basic science, probability theory holds a central place in pure mathematics while also serving a crucial function across various domains, including natural science, social science and engineering technology. This paper summarizes the main concepts, basic theories and commercial applications of probability theory. Probability theory encompasses ideas such as random experiment, sample space, event, probability, conditional probability, independence, Bayes Theorem, random variable, probability distribution, expected value and variance, law of large numbers and central limit theorem. This paper provides an overview of key concepts and theorems from probability theory, along with their practical applications in everyday life, in order to make people deeply recognize the broad and profound contents and research fields of probability theory and its wide application in various fields as an academic tool, which has a great relation with people.

CDC42 deficiency leads to endometrial stromal cell senescence in recurrent implantation failure.

Does the downregulation of cell division cycle 42 (CDC42) protein in endometrial stroma lead to endometrial senescence in patients with recurrent implantation failure (RIF), and what is the potential mechanism? CDC42 deficiency causes endometrial stromal senescence and decidualization defects, impairing uterine receptivity of RIF patients, via activation of Wnt signaling pathway. Uterine aging is unique due to the cyclic remodeling and decidualization of endometrial tissue. Several transcriptomic studies have reported increased senescence in the endometrium in young patients with RIF. Our previous transcriptomic sequencing study discovered that endometrium from women with RIF showed downregulation of CDC42, which is an essential molecule affected by various senescence-related diseases. The endometrial samples of a total of 71 fertile control patients and 37 RIF patients were collected to verify the association between CDC42 expression and endometrial senescence of RIF patients. Primary endometrial stromal cells (EnSCs) were isolated from endometrial biopsies taken from patients without any endometrial complications and planning to undergo IVF, then subjected to adenovirus-mediated CDC42 knockdown and decidualization induction to explore the detailed mechanism by which CDC42 governs stromal senescence and decidualization. Wnt inhibitor XAV-939 was used to correct the endometrial senescence and decidualization defect. Senescence was determined by cell cycle arrest markers (e.g. P16, P21, and P53), SASP molecules (e.g. IL6 and CXCL8), and SA-β-gal staining. Masson's staining and Sirius Red staining were used to detect the endometrial fibrosis. Decidualization was evaluated by the mRNA expression and protein secretion of PRL and IGFBP1, F-actin immunostaining, and the BeWo spheroids 'in vitro implantation' model. Methods used to assess cell function included adenovirus transduction, RNA-sequencing, bioinformatic analysis, western blotting, RT-qPCR, ELISA, and immunofluorescence. Here, we observed remarkably increased levels of stromal senescence and fibrosis, along with stromal CDC42 deficiency, in the endometrium of patients with RIF (P < 0.001). Knockdown of CDC42 effectively induced premature senescence in EnSCs, leading to aberrant accumulation of senescent EnSCs and collagen deposition during decidualization. CDC42 deficiency in EnSCs restrained the decidualization differentiation and receptivity to trophoblast cells. Transcriptomic analysis revealed Wnt signaling activation as a critical downstream alteration in CDC42-deficient EnSCs. Mechanistically, CDC42 interacted with AKT competitively to impede the binding of GSK3β to AKT. Knockdown of CDC42 increased AKT-mediated phosphorylation of GSK3β to inactivate the Axin-GSK3β destruction complex, leading to accumulation and nuclear translocation of β-catenin. Importantly, Wnt signaling inhibitors partially corrected the endometrial senescence caused by CDC42 deficiency, and improved both decidualization and trophoblast invasion. RNA-seq data sets generated in this study have been deposited at the NCBI database with BioProject accession number PRJNA1102745. The present study was based on in vitro cell cultures. Further studies involving CDC42-regulated endometrial senescence are needed in knockout mice model and human endometrial assembloids. In addition to uncovering endometrial senescence in RIF, our findings underscore the significance of CDC42 in modulating EnSC senescence to maintain the decidualization function, and suggest Wnt signaling inhibitors as potential therapeutic agents for alleviating endometrial senescence. This work was supported by the National Natural Science Foundation of China [82271698 (R.J.), 82030040 (H.S.), 82288102 (H.W.), and 82371680 (G.Y.)]; the Natural Science Foundation of Jiangsu Province [BK20231117 (R.J.)]; and the Medical Science and Technology Development Foundation of Nanjing Department of Health [YKK23097 (Y.Z.)]. The authors declare no potential conflicts of interest.

Thyroid-stimulating hormone suppression in low-risk papillary thyroid cancer: a large-scale retrospective analysis of real-world data

Over 500,000 new cases are diagnosed with papillary thyroid cancer (PTC) globally per year, of whom the vast majority are in the low-risk stratification. Although thyroid-stimulating hormone (TSH) suppression is traditionally recommended for all postoperative PTCs in current guidelines, its necessity remains highly controversial in low-risk patients. Since relevant recommendations in current guidelines are still empirical, we aim to provide a direct, large-scale, real-world evidence. This large-scale real-world retrospective study included 11,140 low-risk PTC patients from two Chinese large-volume centers (Fudan University Shanghai Cancer Center [FUSCC] and Cancer Hospital of Chinese Academy of Medical Sciences [CH-CAMS]) treated from January 1, 2000 to June 30, 2022. The mean TSH level was calculated based on postoperative serum TSH values during follow-up. The primary outcome was the association between postoperative TSH level and structural recurrence assessed by Kaplan-Meier, log-rank, multivariate Cox regression analyses and equivalence testing by Two One-Sided Tests (TOST) procedure. Propensity score matching (PSM) was used to adjust for confounders among groups. A total of 11,140 patients with low-risk PTC were included with a median follow-up of 70 months. Based on the mean TSH level, we classified these patients into ≤0.5 (n=1,504, 13.5%), (0.5-1] (n=4,336, 38.9%), (1-2] (n=4,285, 38.5%), (2-3] (n=704, 6.3%) and >3 (n=311, 2.8%) mU/L groups. After PSM adjusting for age, sex, T and N stage, 8991 patients were included in further analysis, for whom the log-rank analyses showed no significant differences between any two groups (all P>0.05) in recurrence-free survival (RFS), locoregional recurrence-free survival (LRRFS) and distant metastasis-free survival (DMFS), and suppressed TSH was not associated with tumor recurrence in the multivariate Cox analysis (TSH > 2 group vs TSH ≤ 2 group: HR=1.30, 95% CI=0.85-2.01, P=0.23). Furthermore, the TOST equivalence tests showed that tumor recurrence status of any two TSH groups were statistically comparable (all Bonferroni-corrected P values<0.005). Subgroup multivariate analyses showed that TSH level did not impact tumor recurrence regardless of age, tumor size, lymph node metastasis, multifocality, surgical extent, biochemical evidence. Our results suggested that postoperative TSH level was not associated with tumor recurrence in patients with low-risk PTC, for whom deliberate TSH suppression may be exempted to avoid potential secondary complications. Maintaining a TSH level within the normal range may be safe for these patients. The study was supported by the National Natural Science Foundation of China (82072951 to Y.W.; 82373008 to X.S.), Shanghai Hospital Development Center (SHDC2020CR6003-001 to Y.W., SHDC2024CRI087 to Y.-J.W.), the Science and Technology Commission of Shanghai Municipality (22Y21900100/23DZ2305600 to Y.W.; 23ZR1412000 to X.S.), the Shanghai Anticancer Association Foundation (SACA-AX202213 to Yu Wang), Shanghai Municipal Health Commission and Shanghai Medicine and Health Development Foundation (WJWRC202302 to X.S.).

Gut microbiome metabolites, molecular mimicry, and species-level variation drive long-term efficacy and adverse event outcomes in lung cancer survivors

The influence of the gut microbiota on long-term immune checkpoint inhibitor (ICI) efficacy and immune-related adverse events (irAEs) is poorly understood, as are the underlying mechanisms. We performed gut metagenome and metabolome sequencing of gut microbiotas from patients with lung cancer initially treated with anti-PD-1/PD-L1 therapy and explored the underlying mechanisms mediating long-term (median follow-up 1167 days) ICI responses and immune-related adverse events (irAEs). Results were validated in external, publicly-available datasets (Routy, Lee, and McCulloch cohorts). The ICI benefit group was enriched for propionate (P=0.01) and butyrate/isobutyrate (P=0.12) compared with the resistance group, which was validated in the McCulloch cohort (propionate P<0.001, butyrate/isobutyrate P=0.002). The acetyl-CoA pathway (P=0.02) in beneficial species mainly mediated butyrate production. Microbiota sequences from irAE patients aligned with antigenic epitopes found in autoimmune diseases. Microbiotas of responsive patients contained more lung cancer-related antigens (P=0.07), which was validated in the Routy cohort (P=0.02). Escherichia coli and SGB15342 of Faecalibacterium prausnitzii showed strain-level variations corresponding to clinical phenotypes. Metabolome validation reviewed more abundant acetic acid (P=0.03), propionic acid (P=0.09), and butyric acid (P=0.02) in the benefit group than the resistance group, and patients with higher acetic, propionic, and butyric acid levels had a longer progression-free survival and lower risk of tumor progression after adjusting for histopathological subtype and stage (P<0.05). Long-term ICI survivors have coevolved a compact microbial community with high butyrate production, and molecular mimicry of autoimmune and tumor antigens by microbiota contribute to outcomes. These results not only characterize the gut microbiotas of patients who benefit long term from ICIs but pave the way for "smart" fecal microbiota transplantation. Registered in the Chinese Clinical Trial Registry (ChiCTR2000032088). This work was supported by Beijing Natural Science Foundation (7232110), National High Level Hospital Clinical Research Funding (2022-PUMCH-A-072, 2023-PUMCH-C-054), CAMS Innovation Fund for Medical Sciences (CIFMS) (2022-I2M-C&T-B-010).

DHDH-mediated D-xylose metabolism induces immune evasion in triple-negative breast cancer.

Although the prognosis of triple-negative breast cancer (TNBC) has significantly improved in the era of immunotherapy, many TNBC patients are resistant to therapies, and their disease progresses rapidly. Deciphering the metabolic mechanisms regulating anticancer immunity will provide new insights into therapeutic strategies for TNBC. In this study, we performed bioinformatics analysis in our multi-omics TNBC database and identified that a metabolic enzyme, dihydrodiol dehydrogenase (DHDH), might promote the phenotype of "cold tumor" in TNBC. The biological function of DHDH was verified by invitro and invivo functional experiments, and the potential molecular mechanism of DHDH promoting TNBC immune escape was further explored. Mechanistically, DHDH mediated the synthesis and depletion of the substrate D-xylose and inhibited the activation of the proteasome subunit beta type 9 (PSMB9) and further induction of the immune response. We demonstrated that D-xylose supplementation could enhance the proliferation of CD8+ Tcells and the expression of cytotoxic markers against cocultured DHDH-wild type (WT) cells. Consistently, D-xylose supplementation invivo promoted CD8+ Tcell infiltration and the expression of cytotoxic markers and increased the sensitivity of DHDH-overexpressing tumors to immune checkpoint blockade (ICB). Our findings reveal that a D-xylose-regulated PSMB9-dependent pathway governs tumor-intrinsic immunogenicity and, hence, the sensitivity to ICB, which may provide approaches to promote the "cold-to-hot" transition in TNBC. This study was funded by the National Key Research and Development Plan of China, Shanghai Science and Technology Commission, National Natural Science Foundation of China, and China Postdoctoral Science Foundation.

THE GOALS AND OBJECTIVES OF INTEGRATED CLASSES IN ELEMENTARY SCHOOL TECHNOLOGY CLASSES AND THE IMPORTANCE OF INTERDISCIPLINARY COMMUNICATION

This article is currently talking a lot about the integration of Primary School Education. This concept is characterized by the perception of the universe around a small schoolboy as a whole, for him not by the name of Natural Science, Russian language, mathematics, reading literacy and other educational sciences, but by the curiosity of the variety of sounds, colors, volumes of objects of the surrounding universe. The teacher feels and knows that students need to be taught to see the connection of nature and everything in everyday life. In the process of passing the subject of tenology, the teacher provides education, upbringing, forms some characteristic of students. It is in the process of fulfilling those goals in practice that several disciplines are addressed.Therefore, questions such as whether educational integration meets the current demand, how to organize it, are gaining relevance.

Single cell atlas reveals multilayered metabolic heterogeneity across tumour types

Metabolic reprogramming plays a pivotal role in cancer progression, contributing to substantial intratumour heterogeneity and influencing tumour behaviour. However, a systematic characterization of metabolic heterogeneity across multiple cancer types at the single-cell level remains limited. We integrated 296 tumour and normal samples spanning six common cancer types to construct a single-cell compendium of metabolic gene expression profiles and identify cell type-specific metabolic properties and reprogramming patterns. A computational approach based on non-negative matrix factorization (NMF) was utilised to identify metabolic meta-programs (MMPs) showing intratumour heterogeneity. In-vitro cell experiments were conducted to confirm the associations between MMPs and chemotherapy resistance, as well as the function of key metabolic regulators. Survival analyses were performed to assess clinical relevance of cellular metabolic properties. Our analysis revealed shared glycolysis upregulation and divergent regulation of citric acid cycle across different cell types. In malignant cells, we identified a colorectal cancer-specific MMP associated with resistance to the cuproptosis inducer elesclomol, validated through in-vitro cell experiments. Furthermore, our findings enabled the stratification of patients into distinct prognostic subtypes based on metabolic properties of specific cell types, such as myeloid cells. This study presents a nuanced understanding of multilayered metabolic heterogeneity, offering valuable insights into potential personalized therapies targeting tumour metabolism. National Key Research and Development Program of China (2021YFA1300601). National Natural Science Foundation of China (key grants 82030081 and 81874235). The Shenzhen High-level Hospital Construction Fund and Shenzhen Basic Research Key Project (JCYJ20220818102811024). The Lam Chung Nin Foundation for Systems Biomedicine.

Challenges Experienced by Teachers when Integrating Indigenous Knowledge Systems in Natural Sciences Practical Work

This study explored what challenges teachers experienced when integrating indigenous knowledge systems (IKS), which are culture specific, into practical work in natural sciences classrooms with diverse learners. Using an exploratory qualitative case study based on an interpretive paradigm, three natural sciences teachers and two subject advisors were purposively selected from the Harry Gwala district in the KwaZulu–Natal province of South Africa. Semi-structured interviews, a focus group interview and practical work lesson observations were used to collect data that were analysed using thematic content analysis. The findings show that when indigenous knowledge was integrated with practical work, real-life activities were simulated in the classrooms. The introduction of real-life situations and IKS-based practical activities invited IKS-based learner behaviour. When simulating real-life practices, the learners’ socio-cultural beliefs became manifest. It was reported that learners assigned traditional gender roles for the practical work activities. Recent initiates into adulthood did not participate in activities that included the use of open fires because it was forbidden in their culture. Learners were also not willing to work with clay soil based on the belief that it attracted goblins, despite evidence showing its use in the building of huts and making of pots. Furthermore, teachers recognised the need to manage the conflicts and controversies that might result from integrating IKS into practical work. The study recommends education districts to establish contextualised resource centres for IKS integration that take into account the socio-cultural beliefs of local learners as a way to support the teachers.

Website-Based Learning Media Using Google Sites to Improve Student Learning Outcomes in Natural and Social Sciences Subjects on Biodiversity Material

Inappropriate use of learning media in learning process activities can cause uninteresting learning. This study aims to develop website-based learning media using Google Sites in the Natural and social sciences subject biodiversity material in grade V elementary school. The research method used is Research and Development with the ADDIE development model. The subjects in this study were media experts, material, teachers, and 21 fifth-grade elementary school students. Data collection is used in the form of test techniques and non-test techniques. The test techniques used are pretest and posttest, while non-test techniques include observation, interviews, questionnaires, and documentation. In the data analysis process, the normality test, t-test, and N-Gain test methods were used, which were carried out with SPSS 23 software. The results of the study presented a validation test by media experts getting a score of 91%, while the validation test by material experts got a score of 92%, with a very feasible category. The t-test results on the small group trial presented a sig. (2-tailed) of 0.008. while in the large group trial, the t-test presents a sig value (2-tailed) of 0.000. The results of the N-Gain Test in the small group and large group trials showed an increase in value of 0.60 and 0.55 which was in the medium category. This shows an average increase between pretest and posttest scores. From these results, it is concluded that website-based learning media using Google Sites is very feasible and effective in improving student learning outcomes related to the biodiversity material in grade V elementary school.

Learning Innovations in Coastal Areas Through Augmented Reality and Gamification

This research investigates the development of an augmented reality (AR) application with gamification elements to enhance natural science learning in coastal areas. Validation by media and content experts, along with trials conducted among students, showed high feasibility and strong appeal of the application. Functionality tests, including marker detection range, demonstrated the application's capability to operate effectively in diverse coastal environments. The study highlights the successful integration of AR and gamification as a tool to improve engagement and understanding of natural science concepts in under-resourced coastal schools. This application holds great potential for enhancing the quality of education in coastal areas by offering an interactive and immersive learning experience. Further research is recommended to refine the application and assess its long-term impact on student comprehension and educational outcomes in various coastal communities. This study represents a significant step forward in using innovative AR technology to support learning in coastal regions.

Analysis of University Students Liberal Arts Curriculum Perception and Needs

This purpose of this study is to analyze the perceptions and needs of students at University C regarding ‘liberal arts education’ and to draw implications for the reorganization and operation of the liberal arts curriculum. To this end, a survey was conducted on the perceptions and needs of the monitoring group of University C, and in-depth interviews and data analysis were conducted. A total of 91 students participated in the survey, responding to their perceptions of liberal arts education in general and their needs, and the researchers reviewed and analyzed 40 final reports submitted by the liberal arts education monitoring group. Five students, three from the humanities/social sciences and two from the natural sciences/ engineering, who were excellent members of the Liberal Arts Monitoring Group, were interviewed in-depth, focusing on information that could not be found in the survey and final reports nor could be confirmed through interviews. The analysis revealed the following perceptions and needs for liberal arts education.&lt;/br&gt;First, students at College C perceived that liberal arts education was necessary overall, but tended to perceive it as less important than education involving their majors. Second, students perceived that the liberal arts curriculum at College C was below average in reflecting their needs. Third, while the survey showed a strong preference for lecture-style classes, the data analysis and in-depth interviews showed that the curriculum should be improved with more interactive teaching and learning methods. Fourth, students at University C felt burdened and fatigued by the relative evaluation method centered on memorization and written evaluation, and demanded a change to an evaluation system that focuses more on the meaning of learning.&lt;/br&gt;Based on these findings, the university's dedicated organization for liberal arts education needs to make efforts to change the students' perceptions of the importance and value of liberal arts education and key information for academic guidance. In addition, efforts should be made to reflect student needs in the reorganization and operation of the liberal arts curriculum by establishing and operating student organizations such as student liberal arts curriculum committees and student liberal arts education monitoring groups, or even through competitions. In addition, the dedicated organization for liberal arts education should support liberal arts instructors and share teaching and learning methods that are more in line with liberal arts education through regular seminars for dedicated faculty members. Finally, it is necessary for educators to improve the evaluation system that focuses on students' learning activities in the student evaluation process, and to increase the satisfaction level of liberal arts courses through ‘clarity of evaluation criteria’ and ‘instructor feedback on grades’, and to improve instructors' awareness of student evaluations and to foster their evaluation expertise.

의사결정나무 분석에 대한 국내 연구 동향 탐색

This study explores the basic system and applicability of decision tree techniques by examining domestic research trends in this field. We analyzed 1,075 academic papers using domestic decision tree analysis from 2001 to 2023, categorized by year, journal, topic field, author, paper frequency, and frequency of algorithms used. Key findings reveal that the frequency of papers began with one in 2001, increasing to 87 in 2023. The distribution across fields was as follows: social science (377 papers, 35.07%), natural science (233 papers, 21.67%), and engineering (230 papers, 21.40%). Among the algorithms, CHAID was used most frequently (315 times, 36.42%), followed by CART (310 times, 35.84%), ensemble techniques like random forest (124 times, 14.34%), and C5.0 (94 times, 10.87%). The Quest algorithm was rarely used (10 times, 1.16%). The annual usage trends indicate a growing preference for ensemble techniques to enhance decision tree prediction rates. The paper's keywords included “decision trees” (687), “data mining” (239), “machine learning” (105), “logistic” (98), and “neural networks” (68). Notably, keywords related to health, such as “suicide,” “depression,” “high blood pressure,” and “health,” emerged prominently, signifying the extensive use of decision trees in medical research. Based on these findings, we propose implications and directions for follow-up studies.

Transformative Sustainable Education Approach as Environmental Literacy Transformation: Case Study of Ecosystem Material in Elementary Schools

The adiwiyata program has been implemented in several elementary schools in Indonesia, but its implementation has yet to be integrated into learning. The objectives of this research are: 1) To find out the implementation of the Transformative Sustainable Education (TSE) approach in Natural and Social Science (NSS) learning; 2) To find out whether the implementation of the TSE approach is effective in improving students' environmental literacy. This TSE approach is implemented in natural and social sciences learning. This research used an experimental method with pretest-posttest control group design. The population of this study were 5th grade students of MIN 2 Bantul which consisted of class A and class B. The sample was selected by cluster random sampling and the students were selected. The sample was selected by cluster random sampling and class A of 29 was selected as the experimental class and class B of 28 as the control class. The instruments used in this study include observation sheets of learning implementation with the TSE approach and environmental literacy observation sheets. The results showed that: 1) The TSE approach was implemented with a percentage of 95% in the very good category; 2) The TSE approach was effective in improving environmental literacy in the medium category.

The destruction of Sciences at Massey University and why we need an independent entity that can investigate universities

This article summarises the events that led to the dismantling of the school of Natural and Computational Sciences at Massey University, Albany campus, and the unsuccessful attempts to prevent it by staff, the public and the New Zealand Association of Scientists (NZAS). Although challenging to prove, we argue that the dismantling was unnecessary, senseless and counterproductive. The unsuccessful attempts to save an academically and financially viable school exposed a weakness in the current Education and Training Act and demonstrated why New Zealand (NZ) needs an independent entity that could investigate universities, guard the national interests, ensure accountability of tertiary institutions’ administration, and protect the academic freedom of individual academics.

The Impact of Flipped Learning on Students` Critical Thinking; A Systematic Literature Review

Flipped learning is a revolutionary teaching method that disrupts traditional learning environments by providing instruction outside of the classroom. This strategy enables students to engage with the subject at their convenience and encourages active learning in the classroom. The current study is a comprehensive literature analysis that looks into the impact of flipped learning on student critical thinking skills. The Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) criteria were utilized to ensure that the article selection was valid and reliable. The articles were selected from some credible databases, such as Eric, Scopus, and ProQuest. After the selection procedure, the systematic review yielded 23 relevant articles to be further reported. Our analysis highlights that flipped learning practices are most commonly observed in natural science disciplines, including physics, mathematics, and chemistry. The majority of the studies employed quantitative methodologies, primarily utilizing quasi-experimental designs, and predominantly focused on university-level students in Asian countries. Notably, the findings indicate a substantial positive effect of online-based flipped learning on the enhancement of students' critical thinking skills, suggesting that this pedagogical approach can significantly benefit student learning outcomes.

TREE ADVENTURE IN ENGLISH: A JOURNEY INTO EXPLORING TREE SPECIES, ENGLISH, AND DIGITAL LITERACY

The presented research demonstrates the use of innovative teaching methods by incorporating cross-curricular integration of natural sciences and English and using digital competencies in the classroom. Students actively developed digital skills through interactive activities while learning about forests in the English language. They expanded their vocabulary on tree species and forests and practiced comprehension based on authentic English-language recordings. Particular emphasis was placed on recognizing tree species based on leaves, fruits, and general characteristics and acquiring the relevant vocabulary in English. Based on the English language video, students learned to distinguish between fir, spruce, and pine. They developed digital skills in various areas and at distinct levels by photographing trees for the film's background, drafting a script, and recording a film, which served as the final product of their learning. This approach connected theoretical knowledge with practical skills, fostering creativity and collaboration. These innovative approaches demonstrate the positive effects of cross-curricular teaching of natural sciences and English and the use of digital competencies in the classroom, allowing students to comprehensively understand the subject matter and contribute to their holistic development in multiple areas. Innovative approaches encourage active participation and the development of language skills and strengthen cooperation. Keywords: digital competencies, cross-curricular integration, English, forest trees, natural sciences

Phase response curves and the role of coordinates.

The "infinitesimal phase response curve" (PRC) is a common tool used to analyze phase resetting in the natural sciences in general and neuroscience in particular. We make the observation that the PRC with respect to a coordinate v actually depends on the choice of other coordinates. As a consequence, a complete delay embedding reconstruction of the dynamics using v which would allow phase to be computed still does not allow the v PRC to be computed. We give a coordinate-free definition of the PRC making this observation obvious. This leads to an experimental protocol: first collect an appropriate ensemble of measurements by intermittently controlling neuron voltage. Then, for any suitable current carrier dynamic postulated, we show how the ensemble can be used to compute the voltage PRC with that current carrier. The approach extends to many oscillators measured and controlled through a subset of their coordinates.

Exploring Food Processing in Natural Science Education: Practical Applications and Pedagogical Techniques

Food processing is an essential link in the chain that connects agricultural production with consumer nutrition and safety. This paper attempts to discuss how effectively food processing can be integrated into the curriculum of natural sciences to further the understanding of students about scientific principles through practical applications. Discussion of relevance to food processing prepares the ground for biology, chemistry, and environmental science, focusing on such core processes as fermentation, pasteurization, and conservation. This paper outlines how reviewing successful strategies and case studies, concept concretization of science is realized through practical activities and interdisciplinary projects, and larger goals are achieved in education for sustainability, food safety, and healthier eating. Besides, it underscores the place of technology and innovation through digital tools and virtual simulation in making the lessons interactive for the students in a career-oriented manner. The article concludes with the following practical suggestions for educators and advocates of the partnership among schools, industry, and community toward an enriched education preparing students with vocational skills.

Crossing the frontier from oral to written translanguaging for epistemic access in natural science

Background: South Africa’s Language-in-Education Policy (LiEP) provides for bi/multilingual education, but schools are only supported to choose monolingual English language policies from Grade 4 and ignore the learners’ home language or languages as resources for learning. Many teachers translanguage orally, using the learners’ home language and English to make learning more meaningful but seldom extend this to written translanguaging.Objectives: Research was conducted to document the process and effects of a teacher development project, translanguaging-for-learning (L4L) which supported teachers in developing translanguaging pedagogies to improve learner access to the curriculum and to develop academic bi-literacy.Method: This article focuses on the development of one senior phase (Grades 7–9) natural science teacher who participated in the L4L project. Data were collected following an ethnographic approach in workshops and classrooms through observation, interviews, photos, videos, and learners’ workbooks.Results: The research illustrates how with workshop engagement, mentoring support and the provision of multilingual dictionaries, a teacher’s bi/multilingual languaging skills were legitimised and mobilised to engage learners in knowledge construction, both orally and in writing. Classroom interaction shifted from being teacher-led to being more learner-centred and bilingual, providing opportunities for biliteracy, and identity affirmation.Conclusion: The findings illustrate improved learning opportunities through planned, systematic translanguaging pedagogies.Contribution: The research suggests a model for bi/multilingual education and teacher development that recognises African languages as important resources for learning to further linguistic equity, access, and social justice.

How does Learning Differentiate with The Socio-Scientific Issue (SSI) Climate Change Improving Environmental Literacy?

Climate change is a complex global problem that requires understanding and action from students. Natural and Social Sciences projects can integrate climate change learning with social and scientific contexts. The preliminary study show that students do not yet have the environmental literacy skills needed to support science and technology projects learning. It also shows that students have difficulty understanding the concept of climate change and its implications for the environment. This research aims to determine the effect of differentiated learning with SSI to increase students ' understanding of climate change. The research method used is mixed methods research using the Exploratory Sequential Mixed Methods Design method. The research results show that implementing climate change differentiation learning with SSI can improve students ' environmental literacy skills. The results of the one-way ANOVA test have a significance value less than 0.05, where there is an increase after implementing differentiation learning. The findings of this research can integrate climate change learning with social and scientific contexts. SSI can help students understand complex issues that have significant social and scientific impact. This can motivate students to take action to overcome problems as an aspect of environmental literacy skills.