Natural Plant Extracts Research Articles

Methanolic Extract of Phoenix Dactylifera Confers Protection against Experimental Diabetic Cardiomyopathy through Modulation of Glucolipid Metabolism and Cardiac Remodeling.

The incidence of cardiovascular disorders is continuously rising, and there are no effective drugs to treat diabetes-associated heart failure. Thus, there is an urgent need to explore alternate approaches, including natural plant extracts, which have been successfully exploited for therapeutic purposes. The current study aimed to explore the cardioprotective potential of Phoenix dactylifera (PD) extract in experimental diabetic cardiomyopathy (DCM). Following in vitro phytochemical analyses, Wistar albino rats (N = 16, male; age 2-3 weeks) were fed with a high-fat or standard diet prior to injection of streptozotocin (35 mg/kg i.p.) after 2 months and separation into the following four treatment groups: healthy control, DCM control, DCM metformin (200 mg/kg/day, as the reference control), and DCM PD treatment (5 mg/kg/day). After 25 days, glucolipid and myocardial blood and serum markers were assessed along with histopathology and gene expression of both heart and pancreatic tissues. The PD treatment improved glucolipid balance (FBG 110 ± 5.5 mg/dL; insulin 17 ± 3.4 ng/mL; total cholesterol 75 ± 8.5 mg/dL) and oxidative stress (TOS 50 ± 7.8 H2O2equiv./L) in the DCM rats, which was associated with preserved structural integrity of both the pancreas and heart compared to the DCM control (FBG 301 ± 10 mg/dL; insulin 27 ± 3.4 ng/mL; total cholesterol 126 ± 10 mg/dL; TOS 165 ± 12 H2O2equiv./L). Gene expression analyses revealed that PD treatment upregulated the expression of insulin signaling genes in pancreatic tissue (INS-I 1.69 ± 0.02; INS-II 1.3 ± 0.02) and downregulated profibrotic gene expression in ventricular tissue (TGF-β 1.49 ± 0.04) compared to the DCM control (INS-I 0.6 ± 0.02; INS-II 0.49 ± 0.03; TGF-β 5.7 ± 0.34). Taken together, these data indicate that Phoenix dactylifera may offer cardioprotection in DCM by regulating glucolipid balance and metabolic signaling.

Ginkgetin improved experimental colitis by inhibiting intestinal epithelial cell apoptosis through EGFR/PI3K/AKT signaling.

Excessive apoptosis of intestinal epithelial cells leads to intestinal barrier dysfunction, which is not only one of the pathological features of inflammatory bowel disease (IBD) but also a therapeutic target. A natural plant extract, Ginkgetin (GK), has been reported to have anti-apoptotic activity, but its role in IBD is unknown. This study aimed to explore whether GK has anti-colitis effects and related mechanisms. An experimental colitis model induced by dextran sulfate sodium (DSS) was established, and GK was found to relieve colitis in DSS-induced mice as evidenced by improvements in weight loss, colon shortening, Disease Activity Index (DAI), macroscopic and tissue scores, and proinflammatory mediators. In addition, in DSS mice and TNF-α-induced colonic organoids, GK protected the intestinal barrier and inhibited intestinal epithelial cell apoptosis, by improving permeability and inhibiting the number of apoptotic cells and the expression of key apoptotic regulators (cleaved caspase 3, Bax and Bcl-2). The underlying mechanism of GK's protective effect was explored by bioinformatics, rescue experiments and molecular docking, and it was found that GK might directly target and activate EGFR, thereby interfering with PI3K/AKT signaling to inhibit apoptosis of intestinal epithelial cells invivo and invitro. In conclusion, GK inhibited intestinal epithelial apoptosis in mice with experimental colitis, at least in part, by activating EGFR and interfering with PI3K/AKT activation, explaining the underlying mechanism for ameliorating colitis, which may provide new options for the treatment of IBD.

Some Chemical and Biological Methods to Control Pepper Root Rot Disease in Iraq

Background: Pepper growing fields in the Babil and Wasit governorates in Iraq, are suffering from a decline in production due to the spread of root rot diseases. The use of biological control techniques, such as the use of beneficial bacteria in the rhizosphere and natural plant extracts, along with chemical fungicides, has recently contributed to reducing the damage of plant pathogens globally. Methods: In this research, the main cause of root rot disease in pepper plants was isolated and diagnosed using polymerase chain reaction technology using (TEF-1α) gene, antagonistic activities of 75 rhizobacterial isolates, natural extracts of garlic and nerium and the fungicides of Promess and Tecto 500 SC were tested in vitro against the pathogen, then the superior bacterial isolates were identified. The ability of biological and chemical agents to control the disease was tested under greenhouse conditions according to the completely randomized design (CRD). Result: The results of isolation showed that the fungus Fusarium solani was dominance and the isolate Fsw45 was the most virulent. 3 of 75 rhizobacterial isolates outperformed the pathogen in an antagonistic tests and identified as Pseudomonas mendocina, P. fluorescens and Staphylococcus kloosii also the fungicides and botanical extracts showed significant antagonistic activities as well in vitro. Under greenhouse conditions, the fungicide treatments excelled in disease control by 100%. As for the 3 bacterial isolates, they achieved a significant reduction in the percentage of disease incidence and disease severity index, their abilities were higher than the nerium and garlic extracts.

Application of Natural Antibacterial Plants in the Extraction of Uranium from Seawater.

Marine biofouling directly affects the performance and efficiency of uranium (U(VI)) extraction from seawater. Compared to traditional chemical methods, natural plant extracts are generally biodegradable and nontoxic, making them an environmentally friendly alternative to synthetic chemicals in solving the marine biofouling problem. The effectiveness of natural antibacterial plants (i.e., pine needle, peppermint, Acorus gramineus Soland, Cacumen platycladi, and wormwood) in solving the marine biofouling problem was evaluated in this work. Experimental results showed that natural antibacterial plants could kill Vibrio alginolyticus in solution and effectively solve the marine biofouling problem of U(VI) extraction. To improve the adsorption capacity of natural plants for U(VI) in seawater, poly(vinylphosphonic acid) (PVPA) was modified on natural antibacterial plant surfaces by irradiation grafting technology. PVPA and natural antibacterial plants work as active sites and base materials for the U(VI) extraction material, respectively. The recovery performance of PVPA/pine needle for U(VI) was preliminarily studied. Results show that the adsorption of U(VI) on PVPA/pine needle follows pseudo-second-order and Langmuir models, and the maximum adsorption capacity is 111 mg/g at 298 K and pH 8.2.

Lycopene alleviates Deoxynivalenol-induced toxicity in Porcine intestinal epithelial cells by mediating mitochondrial function

Deoxynivalenol (DON) is widely found in food and feed, posing a threat to human and animal health. Lycopene (Lyc) is a natural plant extracts with significant antioxidant properties. This study was conducted to investigate the protective effects of Lyc on IPEC-J2 cells upon DON exposure. The detection of cell viability and trypan blue staining showed that Lyc alleviated cell damage and decreased cell apoptotic rate induced by DON. The analysis of reactive oxygen species (ROS) level and antioxidant parameter measurements showed that Lyc significantly down-regulated the content of ROS and restored antioxidant enzyme activity. Furthermore, mitochondrial membrane potential (ΔΨm) detection, mitochondrial DNA copy number (mtDNAcn) assay and adenosine triphosphate (ATP) concentration detection showed Lyc improved mitochondrial function after DON exposure. The results of transcriptome analysis, ROS detection and CCK8 assay suggested that Lyc may activated the oxidative phosphorylation (OXPHOS) to improve mitochondrial function. Conclusively, our results suggested that Lyc alleviated DON-induced oxidative stress by improving mitochondrial function through OXPHOS signaling pathway.

Phytogenic cocktails fed in different feeding regimes as alternatives to antibiotics for improving performance, intestinal microbial, and carcass characteristics of slow growth chickens.

The phytogenic cocktail (PC) is a unique combination of natural plant extracts consisting of coconut shell smoke, clove leaf extract, and mangosteen rind extract, predominantly containing phenol, eugenol, and α-mangostin. Chicken performance can be improved by its antibacterial properties. This study aimed to test PC as a replacement for antibiotic growth promoters (AGPs), assessing its impact on performance, intestinal microbes, and carcass traits in slow growth KUB chickens. Two hundred and forty KUB chicks were distributed randomly to five dietary groups. Each group constituted six replicates, one replicate contained eight chicks. The treatments included the control diet (CD) with no additives, CD with 50 ppm Zinc bacitracin as an additive (AGPs), CD paired with 198 mL PC/ton feed provided for the initial 12 weeks (PC1), CD with 198 mL PC/ton feed given for the first 4 weeks (PC2), and CD supplied with 198 mL PC/ton feed for the first 8 weeks (PC3). Performance and mortality indicators were assessed during the feeding stage up to 12 weeks of age, while intestinal total microbial count and carcass characteristics were determined at 12 weeks. Duncan's multiple-range test identified differences among the treatments in the randomized experiment. The AGPs group weighed significantly more (p < 0.05) than PC2 but not significantly different (p > 0.05) from Control, PC1, and PC3 at 4 weeks. At 8 weeks, there was no significant difference (p > 0.05) in the body weight (BW) between the AGP, CD, and PC groups. The AGPs group had a significantly greater BW than PC1 and PC2 at 12 weeks (p < 0.05), but was comparable to CD and PC3 (p > 0.05). During the starter phase (0-4 weeks), dietary addition of AGPs or PCs significantly reduced feed intake (p < 0.05); however, no significant effect (p > 0.05) was observed during the later feeding periods (0-8 or 0-12 weeks). During the starter period, PC3 yielded the best feed conversion ratio, slightly surpassing AGPs and significantly (p < 0.05) outperforming CD. No significant variations (p > 0.05) were detected in the carcasses among the treatments. The reduction of abdominal fat relative weight was significant (p < 0.05) during the first 8 weeks of PC feeding. After the 12-week trial, no significant difference (p > 0.05) was observed in the proportionate weights of the crop, proventriculus, gizzard, pancreas, cecum, spleen, bursa of Fabricius, heart, and liver. The reduction in the intestinal microbe population due to AGPs or PC was not statistically significant (p > 0.05). About 100% viability was confirmed by the absence of mortality throughout the study. PC supplementation in KUB chicken feed enhances their performance. The optimal feeding regimes were effective during the first 8 weeks of age. In the 0-4 week time frame, feeding the PC to the chicken worsened performance whereas no improvement was observed in the 0-12 week period. The application enhanced weight loss, feed efficiency, and reduced abdominal fat. Based on the research findings, the PC can replace AGPs as a feed additive due to comparable or superior improvement results.

The Lethal Effect of Some Natural Plants Extracts on Tobacco cutworm Spodoptera litura (Lepidoptera: Noctuidae) and their Relative Toxicity

The Lethal Effect of Some Natural Plants Extracts on Tobacco cutworm Spodoptera litura (Lepidoptera: Noctuidae) and their Relative Toxicity

Potential of Cosmos caudatus Kunth. extract as natural food sanitiser

Bacterial growth in food sources after harvesting and during food manufacturing has emerged as a major agricultural concern. Currently, several chemical preservatives have been added to foods to prevent or inhibit bacterial growth. These phenomena, however, become a concern when consumers begin to demand the safety of the food they consume, thus resulting in a search for natural plant extracts as an alternative. The antimicrobial activity of Cosmos caudatus leaf extract against six food pathogens was thus investigated in the present work. The disc-diffusion test, the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC) tests were used to determine the antibacterial potential. Time-kill kinetics were used to determine their killing endpoints at various incubation times. Thereafter, Bacillus cereus was selected as a representative bacterium to be viewed under the light sheet electron microscope (LSEM) to observe the morphological changes. For the application as a sanitiser, different concentrations of C. caudatus extract were applied to chicken meat and oyster mushroom to determine bacterial reduction and sensory acceptability. The toxicity test using brine shrimp lethality assay was also performed to determine the toxicity level of C. caudatus extract. Results showed that C. caudatus extract contain five major peaks of flavonoids. In the antibacterial test, all tested pathogens were inhibited, with B. cereus being the most susceptible, with a significant reduction time of more than 3 log10 at 3.125 mg/mL. Under LSEM, the population of B. cereus was reduced, and the shape was wrinkled. In the application, C. caudatus extract at 0.05% for 5 min treatment time was chosen as the best formulation, whereas the brine shrimp tests showed that C. caudatus extract was safe for human consumption (LC50: 3.54 mg/mL). In conclusion, C. caudatus extract could have the potential to be further explored as a natural food sanitiser.

Chemical constituents and bioactivities of fermented rose (from Yunnan) extract

Natural plant extracts have gained significant attention in research due to their low toxicity, and potent antioxidant, and anti-aging properties. The present study investigated the phytochemical composition of a fermented rose extract (FRE), and evaluated its antioxidant, skin whitening, and anti-aging activities in vitro. The results showed that the FRE was rich in polyphenols and flavonoids. A total of 13 major compounds were identified by Liquid Chromatography-Mass Spectrometry (LC-MS), with astragalin as the primary component. In vitro, analysis of antioxidant activity showed that FRE effectively eliminated 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals and dose-dependent reduced intracellular reactive oxygen species (ROS) levels. The FRE dose-dependent inhibited tyrosinase, collagenase, and hyaluronidase activity, reduced intracellular melanin synthesis, up-regulated the expression of collagen type I alpha 1 (COL1A1) and collagen type III alpha 1 (COL3A1), and down-regulated matrix metalloproteinases (MMPs) expression. Additionally, treatment with FRE significantly downregulated the expression of mitogen-activated protein kinase 1 (MAPK1), suggesting that FRE may modulate MAPK signaling pathways for skin anti-aging.

Combining Different Natural Plant Extracts to Stabilize the Antioxidative Activity of Dragon's Blood.

Dragon's blood () is a traditional Chinese medicine known for its wound hemostasis, blood circulation, and stasis properties. Recently, it has also been utilized in cosmetics, though its antioxidant capacity remains unclear. This study aims to stabilize the bioactivity of dragon's blood using various plant extracts. We evaluated single plant extracts and their combinations to identify the conditions that maintained the antioxidant capacity of dragon's blood the longest. Selected plants included Hibiscus sabdariffa, Clitoria ternatea, Hylocereus sp., Pandanus amaryllifolius, and Camellia sinensis. We used two sources of dragon's blood: Daemonorops draco and Dracaena cochinchinensis. Extraction conditions were optimized and antioxidant activity was assessed using the free radical scavenging ability of 1,1-diphenyl-2-picrylhydrazyl (DPPH), total anthocyanin concentration (TAC), total polyphenol content (TPC), the free radical scavenging activity of ABTS, and a ferric reducing antioxidant power (FRAP) assay. The results showed that all plant extracts exhibited high antioxidant capacity. Clitoria ternatea had the highest DPPH scavenging ability at 93.81%, with the best combination being green tea and Daemonorops draco at 92.57%. Clitoria ternatea had the highest TPC at 9921 mg GAE/100 g, with the best combination (green tea and Dracaena cochinchinensis) at 10500 mg GAE/100 g. ABTS activity was highest for green tea at 98.3%, with the best combination (Clitoria ternatea and Daemonorops draco) at 93.29%. The FRAP assay showed that green tea had the highest electron-donating potential at 3.85 mg/mL, with the best combination (Daemonorops draco and Dracaena cochinchinensis) at 3.71 mg/mL. This study advances our understanding of the antioxidant properties of these plants and the traditional Chinese medicine dragon's blood, enhancing the efficacy of dragon's blood in skincare and cosmetics. Moreover, the application of these extracts could rejuvenate local agriculture, impacting the skincare, cosmetics, and sustainable agriculture sectors.

Sex differences in the effects of aromatherapy on anxiety and salivary oxytocin levels.

Aromatherapy is a holistic healing method to promote health and well-being by using natural plant extracts. However, its precise mechanism of action and influence on the endocrine system remains unclear. Since recent studies reported that a neuropeptide, oxytocin, can attenuate anxiety, we hypothesized that if oxytocin secretion is promoted through aromatherapy, it may improve mood and anxiety. The present study is aimed to investigate the relationship between oxytocin and the effects of aromatherapy with lavender oil on anxiety level, by measuring salivary oxytocin levels in healthy men and women. We conducted a randomized open crossover trial in 15 men and 10 women. Each participant received a placebo intervention (control group) and aromatherapy with lavender oil (aromatherapy group). For the aromatherapy group, each participant spent a 30-min session in a room with diffused lavender essential oil, followed by a 10-min hand massage using a carrier oil containing lavender oil. Anxiety was assessed using the State-Trait Anxiety Inventory (STAI) before the intervention, 30-min after the start of intervention, and after hand massage, in both groups. Saliva samples were collected at the same time points of the STAI. In women, either aromatherapy or hand massage was associated with a reduction in anxiety levels, independently. Moreover, salivary oxytocin levels were increased after aromatherapy. On the other hand, in men, anxiety levels were decreased after aromatherapy, as well as after hand massage, regardless of the use of lavender oil. However, there were no significant differences in changes of salivary oxytocin levels between the control and aromatherapy groups during the intervention period. Interestingly, there was a positive correlation between anxiety levels and salivary oxytocin levels before the intervention, but a negative correlation was observed after hand massage with lavender oil. The results of the present study indicate that in women, aromatherapy with lavender oil attenuated anxiety with increase in oxytocin level in women, whereas in men, there was no clear relationship of aromatherapy with anxiety or oxytocin levels but, there was a change in correlation between anxiety and oxytocin. The results of the present study suggest that the effect of aromatherapy can vary depending on sex.

Targeting the STAT3/IL-36G signaling pathway can be a promising approach to treat rosacea

BackgroundRosacea is an inflammatory skin disorder characterized by the release of inflammatory mediators from keratinocytes, which are thought to play a crucial role in its pathogenesis. Despite an incidence of approximately 5.5%, rosacea is associated with a poor quality of life. However, as the pathogenesis of rosacea remains enigmatic, treatment options are limited. ObjectivesTo investigate the pathogenesis of rosacea and explore new therapeutic strategies. MethodsTranscriptome data from rosacea patients combined with immunohistochemical staining were used to investigate the activation of STAT3 in rosacea. The role of STAT3 activation in rosacea was subsequently explored by inhibiting STAT3 activation both in vivo and in vitro. The key molecules downstream of STAT3 activation were identified through data analysis and experiments. Dual-luciferase assay and ChIP-qPCR analysis were used to validate the direct binding of STAT3 to the IL-36G promoter. DARTS, in combination with experimental screening, was employed to identify effective drugs targeting STAT3 for rosacea treatment. ResultsSTAT3 signaling was hyperactivated in rosacea and served as a promoter of the keratinocyte-driven inflammatory response. Mechanistically, activated STAT3 directly bind to the IL-36G promoter region to amplify downstream inflammatory signals by promoting IL-36G transcription, and treatment with a neutralizing antibody (α-IL36γ) could mitigate rosacea-like inflammation. Notably, a natural plant extract (pogostone), which can interact with STAT3 directly to inhibit its activation and affect the STAT3/IL36G signaling pathway, was screened as a promising topical medication for rosacea treatment. ConclusionsOur study revealed a pivotal role for STAT3/IL36G signaling in the development of rosacea, suggesting that targeting this pathway might be a potential strategy for rosacea treatment.

Phyllanthus niruni (Meniran) Aqueous and Ethanol Extracts Promotes Adipogenic Differentiation in 3T3-L1 Preadipocytes

Abstract Consumers determine intramuscular fat as one of the important factors for the quality and palatability of beef meat. In a recent study, Phyllanthus niruri (meniran) has practical uses for feed additives and was screened for their ability to improve preadipocyte proliferation and differentiation. This study evaluates the effect of meniran aqueous and ethanol extracts on promoting adipogenic differentiation in 3T3-L1 preadipocytes and were investigated via CCK-8 assay, Oil red O-Staining, and RT-PCR analysis. For differentiation to adipocytes, post-confluent 3T3-L1 preadipocytes were stimulated DMEM containing 10% FBS, 5 μg/ml insulin, 0.5mM 3-isobutyl-1-methylxanthine, and 1 μM dexamethasone. 3T3-L1 were cultured with meniran aqueous and ethanol extract (25, 50, 100, and 200 ppm). Intracellular lipid accumulation was significantly increased by meniran extract until 100 ppm and decreased at 200 ppm. The expressions of adipocyte differentiation on mRNA transcription factors PPARγ and C/EBPꭤ were stimulated by 25-100 ppm of meniran both aqueous and ethanol extract but lower at 200 ppm. In the future, these findings may be applied to develop feed additives from natural plant extracts which may be used to increase intramuscular fat accumulation in beef cattle through manipulation of adipocyte differentiation.

Progress in the treatment of Osteoarthritis with avocado-soybean unsaponifiable.

Osteoarthritis (OA) is one of the leading causes of joint dysfunction and disability in the elderly, posing serious social problems and a huge socio-economic burden. Existing pharmacological treatments have significant drawbacks, and searching for an effective pharmacological intervention is an urgent priority. Recent studies have demonstrated the chondroprotective, anabolic, and anti-catabolic properties of avocado-soybean unsaponifiable (ASU), a natural plant extract made from avocado and soybean oils, consisting of the remainder of the saponified portion of the product that cannot be made into soap. The main components of ASU are phytosterols, beta-sitosterol, canola stanols, and soya stanols, which are rapidly incorporated into cells. Studies have confirmed the anti-inflammatory, antioxidant, and analgesic properties of phytosterols. ASU slows down the progression of OA primarily by inhibiting pathways involved in the development of OA disease. ASU prevents cartilage degradation by inhibiting the release and activity of matrix metalloproteinases and by increasing the tissue inhibition of these catabolic enzymes; ASU is also involved in the inhibition of the activation of nuclear factor κB (NF-κB) which is a transcriptional inhibitor that regulates the inflammatory response of chondrocytes. NF-κB is a transcription factor that regulates the inflammatory response of chondrocytes, and inhibition of the transfer of the transcription factor NF-κB from the cytoplasm to the nucleus regulates the transcription of many pro-inflammatory factors. By appealing to the mechanism of action and thus achieving anti-inflammatory, anti-catabolic, and pro-synthetic effects on cartilage tissues, AUS is clinically responsive to the reduction of acute pain and OA symptom progression. This paper aims to summarize the studies on the use of avocado-soybean unsaponifiable in the pharmacological treatment of osteoarticular.

Endogenous glucose-driven cascade reaction of nano-drug delivery for boosting multidrug-resistant bacteria-infected diabetic wound healing

Multidrug-resistant (MDR) bacteria-infected wound healing remains greatly challenging, especially in diabetic patients. Herein, a novel nano-drug delivery based on endogenous glucose-driven cascade reaction is proposed for boosting MDR bacteria-infected diabetic wound healing with high efficacy by improving wound microenvironment and enhancing photodynamic antibacterial activity. The composite nanoagent is first self-assembled by integrating berberine (BBR) and epigallocatechin gallate (EGCG) from natural plant extracts, named as BENPs, which is successively coated with manganese dioxide nanoshells (MnO2 NSs) and glucose oxidase (GOX) to form the final BEMGNPs. The cascade reaction is triggered by glucose at the wound site of diabetes which is specifically catalyzed by GOX in the BEMGNPs to produce gluconic acid and hydrogen peroxide (H2O2). That is subsequently to decompose MnO2 NSs in the BEMGNPs to generate oxygen (O2). The BEMGNPs as photosensitizers effectively produce reactive oxygen species (ROS) to enhance the eradication of bacteria with the assistance of O2. Under the synergistic function of the cascaded reaction, the BEMGNPs present excellent antibacterial efficacy even for MDR bacteria. The in vivo experiments explicitly validate that the constructed nano-drug delivery can augment the MDR bacteria-infected diabetic wound healing with excellent biosafety. The as-proposed strategy provides an instructive way to combat ever-threatening MDR bacteria, which particularly is beneficial for diabetic patients.

Dictyostelium discoideum -assisted pharmacognosy of plant resources for discovering antivirulence molecules targeting Klebsiella pneumoniae

The rise of antibiotic-resistant bacterial strains represents an important challenge for global health, underscoring the critical need for innovative strategies to confront this threat. Natural products and their derivatives have emerged as a promising reservoir for drug discovery. The social amoeba Dictyostelium discoideum is a potent model organism in this effort. Employing this invertebrate model, we introduce a novel perspective to investigate natural plant extracts in search of molecules with potential antivirulence activity. Our work established an easy-scalable developmental assay targeting a virulent strain of Klebsiella pneumoniae, with Helenium aromaticum as the representative plant. The main objective was to identify tentative compounds from the Helenium aromaticum extract that attenuate the virulence of K. pneumoniae virulence without inducing cytotoxic effects on amoeba cells. Notably, the methanolic root extract of H. aromaticum fulfilled these prerequisites compared to the dichloromethane extract. Using UHPLC Q/Orbitrap/ESI/MS/MS, 63 compounds were tentatively identified in both extracts, 47 in the methanolic and 29 in the dichloromethane, with 13 compounds in common. This research underscores the potential of employing D. discoideum-assisted pharmacognosy to discover new antivirulence agents against multidrug-resistant pathogens.

Camellia sinensis-mediated green synthesis of ZnO nanoparticles: An eco-friendly approach for high-performance gas sensing

This study investigates a sustainable method to synthesize zinc oxide nanoparticles (ZnO NPs) by employing natural plant extracts, specifically utilizing green tea powder commonly known as Camellia sinensis (ZnO synthesized via green tea – ZGT). The research further explores the potential application of these nanoparticles in effective gas sensing. The co-precipitation technique is employed for synthesizing ZnO NPs, utilizing the inherent characteristics of Camellia sinensis, a tea plant. This method ensures the production process is both environmentally sustainable and conscious. The synthesized nanoparticles undergo various analysis, including structural examination through XRD and Raman studies, morphological investigation via SEM with EDX, optical analysis using UV-Vis, and determination of surface material composition through XPS. The average grain size and lattice distortion of the substances were determined using the Debye–Scherrer and Williamson-Hall methods, respectively. The ZGT sensor exhibited notable selectivity and sensitivity to different volatile organic compounds (VOCs) such as Ammonia-NH3, Acetone-C3H6O, Formaldehyde-HCHO, and Benzene-C6H6. The environmentally friendly synthesis route presented in this paper not only offers a greener alternative to conventional methods but also opens paths for the utilization of bio-derived materials in advanced sensing applications.

Celastrus paniculatus seeds as a green corrosion inhibitor for stainless steel in H2SO4 acidic solution

Corrosion poses a significant threat to the durability and safety of metallic structures, particularly in aggressive environments such as sulfuric acid solutions. In this study, the corrosion inhibition performance of Celastrus paniculatus for steel in 0.5 M H2SO4 is investigated. Celastrus paniculatus, a natural plant extract known for its potential corrosion-inhibitive properties, is explored as a green alternative to synthetic inhibitors.The experimental methodology involves the immersion of steel specimens in 0.5 M H2SO4 solutions containing various concentrations of Celastrus paniculatus extract. Electrochemical techniques, such as potentiodynamic polarization and electrochemical impedance spectroscopy, assess corrosion inhibition efficiency. Surface analysis techniques, i.e., scanning electron microscopy (SEM), were employed to investigate the steel surfaces' morphological changes and elemental composition also Quantum Chemical Computations were conducted to find the energy levels of the Lowest Unoccupied Molecular Orbital (ELUMO) and Highest Occupied Molecular Orbital (EHOMO) and the energy gap (ΔE) between HUMO and LUMO from the optimized structure to find of which phytochemical absorb on the surface of the metal and this, we can clear that lower energy containing phytochemical will absorb more on the metal surface and have their greater efficiency. The study contributes to the development of environmentally friendly and sustainable corrosion inhibitors for steel in corrosive media, with potential applications in various industrial sectors. In metallic industries during acid cleaning or pickling processes metal undergoes acidic corrosion. This acidic corrosion can be prevented by many ways including alloying or dealloying of metals, anodic and cathodic protection or application of inhibitors. In this study, we have used the Celastrus paniculatus seed extract. Although its medicinal applications were explored but it was not used as a anticorrosive agent. So here we have determined the anticorrosive effects of Celastrus paniculatus seed in 0.5 M H2SO4 and used this as a novel and highly effective during acid cleaning.

The Efficacy of natural plant extracts in controlling rice weevil (Sitophilus oryzae)

The Efficacy of natural plant extracts in controlling rice weevil (Sitophilus oryzae)

A Review On The Antifungal, Antimicrobial And Antioxidant Effects Of Artichoke Leaf And Grapefruit

Natural plant extracts have become a popular topic nowadays. The antioxidant, antifungal and antimicrobial effects of plants have been investigated by many researchers. It is thought that plants, plant extracts and plant wastes can be used as natural preservatives. In this review, the antifungal, antimicrobial and antioxidant properties of artichoke peel and grapefruit extract were investigated. It is thought that the waste parts of the extracts can be recovered in this way and the plant extracts can be used as natural preservatives.