Natural Aroma Research Articles

Long-term exposure to polycyclic musk tonalide - A potential threat to juvenile zebrafish (Danio rerio)?

Polycyclic musk compounds are commonly used in personal care products to replace expensive natural fragrances. Due to their huge consumption, they have become a part of the aquatic environment. Inthe present study, atwo-month exposure effect oftonalide onjuvenile zebrafish (Danio rerio) was investigated. Wedetermined thevitellogenin concentration todefine the potential endocrine-disrupting effect oftonalide and also analysedselected indices toevaluate the induction ofoxidative stress. The environmentally relevant concentration oftonalide (i.e., 500ng/l) caused asignificant decrease inthe catalase activity (P<0.05) and asignificant increase (P<0.05) inthe lipid peroxidation. Anincreasing lipid peroxidation was also recorded for the highest concentration group tested (i.e., 50000ng/l). Onthe other hand, nosignificant changes were recorded invitellogenin inall theexposed groups. Thus, based onthese results, wehave not demonstrated the endocrine-disrupting activity oftonalide inzebrafish. The results ofthe oxidative stress indices showed asignificant impairment oftheantioxidant defence after the two-month tonalide exposure, which could indicate part ofthe adaptive response tothetonalide toxicity.

Biotechnologies in Perfume Manufacturing: Metabolic Engineering of Terpenoid Biosynthesis.

The fragrance industry is increasingly turning to biotechnology to produce sustainable and high-quality fragrance ingredients. Microbial-based approaches have been found to be particularly promising, as they offer a more practical, economical and sustainable alternative to plant-based biotechnological methods for producing terpene derivatives of perfumery interest. Among the evaluated works, the heterologous expression of both terpene synthase and mevalonate pathway into Escherichia coli has shown the highest yields. Biotechnology solutions have the potential to help address the growing demand for sustainable and high-quality fragrance ingredients in an economically viable and responsible manner. These approaches can help compensate for supply issues of rare or impermanent raw materials, while also meeting the increasing demand for sustainable ingredients and processes. Although scaling up biotransformation processes can present challenges, they also offer advantages in terms of safety and energy savings. Exploring microbial cell factories for the production of natural fragrance compounds is a promising solution to both supply difficulties and the demand for sustainable ingredients and processes in the fragrance industry.

Design of frontal chromatography separation of 1-phenylethanol and acetophenone using a hydrophobic resin

Adsorption and chromatographic processes havegood potential in the purification of natural aromas produced via biocatalytic routes. This work deals with the recovery of 1-phenylethanol (PE) from mixtures containing different amounts of acetophenone (AP). Amberlite FPX68, an industrial adsorbent with high selectivity with respect to acetophenone, was chosen for the separation of PE and AP by frontal chromatography. Small-scale experiments were performed for two different feed compositions and various flow rates and bed lengths. Using the target purity of 99 %, promising yields close to 90 % were obtained. The breakthrough curves were fitted with the equilibrium dispersive model, which provided HETP values under the different conditions. The obtained dependences of HETP on flow velocity complied with the Van Deemter equation. The contributions of individual phenomena responsible for adsorption zone broadening were analyzed. The experimental and modelling results obtained for the small-scale column became the basis for the scale-up of frontal chromatography separation of PE with an annual capacity up to 500 kg.

Loss of Rose Fragrance under Chilling Stress Is Associated with Changes in DNA Methylation and Volatile Biosynthesis.

Rose plants are widely cultivated as cut flowers worldwide and have economic value as sources of natural fragrance and flavoring. Rosa 'Crimson Glory', whose petals have a pleasant fragrance, is one of the most important cultivars of edible rose plants. Flower storage at low-temperature is widely applied in production to maintain quality; however, chilling results in a decrease in aromatic volatiles. To determine the molecular basis underlying the changes in aromatic volatile emissions, we investigated the changes in volatile compounds, DNA methylation patterns, and patterns of the transcriptome in response to chilling temperature. The results demonstrated that chilling roses substantially reduced aromatic volatile emissions. We found that these reductions were correlated with the changes in the methylation status of the promoters and genic regions of the genes involved in volatile biosynthesis. These changes mainly occurred for CHH (H = A, T, or C) which accounted for 51% of the total methylation. Furthermore, transcript levels of scent-related gene Germacrene D synthase (RhGDS), Nudix hydrolase 1 (RhNUDX1), and Phenylacetaldehyde reductase (RhPAR) of roses were strikingly depressed after 24 h at low-temperature and remained low-level after 24 h of recovery at 20 °C. Overall, our findings indicated that epigenetic regulation plays an important role in the chilling tolerance of roses and lays a foundation for practical significance in the production of edible roses.

PKM DEVELOPMENT OF HOUSEHOLD BUSINESS AROMATHERAPY BRICKETS KHOLY ZAINI

People of Martapura City and its surroundings often use incense/bukhur as an air freshener for religious activities. One of the home businesses that produce it is Kholy Zaini. The business was founded 12 years ago with the premise that when pioneering it was that people in the city liked incense/bukhur, while most of it was obtained from imports from the island of Java. Even though it has been running for a long time until now there is still no IPRT and in its manufacture, I t still uses synthetic fragrances, which hurts health. This program is implemented to provide understanding and solutions to partners so that the products produced don’t negatively impact the future. The methods implemented include socialization activities, counseling, discussions, training, and mentoring. The solution that the PKM Team provides to partners is to replace synthetic fragrances with natural fragrances obtained from the distillation of ylang flowers. The process used to make incense/bukhur from natural fragrances is like making it in general, but the difference is da distillation of ylang flowers to get a natural fragrance. The PKM team succeeded in making a natural fragrance from the distillation of ylang flowers. In addition, the PKM team also teaches the company's bookkeeping to partners and develops a website to market their products. The advantages of this PKM activity are making incense/bukhur more environmentally friendly, partners can make the right decisions and increasing sal, es and making products known more widely.

De Novo Biosynthesis of Anisyl Alcohol and Anisyl Acetate in Engineered Escherichia coli.

Anisyl alcohol and its ester anisyl acetate are both important fragrance compounds and have a wide range of applications in the cosmetics, perfumery, and food industries. The currently commercially available anisyl alcohol and anisyl acetate are based on chemical synthesis. However, consumers increasingly prefer natural fragrance compounds. Therefore, it is of great significance to construct microbial cell factories to produce anisyl alcohol and anisyl acetate. In this study, we first established a biosynthetic pathway in engineered Escherichia coli MG1655 for the production of anisyl alcohol from simple carbon sources. We further increased the anisyl alcohol production to 355 mg/L by the increasing availability of erythrose-4-phosphate and phosphoenolpyruvate. Finally, we further demonstrated the production of anisyl acetate by overexpressing alcohol acetyltransferase ATF1 for the subsequent acetylation of anisyl alcohol to produce anisyl acetate. To our knowledge, this is the first report on the biosynthesis of anisyl alcohol and anisyl acetate directly from a renewable carbon source.

Plant Terpenoid Permeability through Biological Membranes Explored via Molecular Simulations.

Plants synthesize small molecule diterpenes composed of 20 carbons from precursor isopentenyl diphosphate and dimethylallyl disphosphate, manufacturing diverse compounds used for defense, signaling, and other functions. Industrially, diterpenes are used as natural aromas and flavoring, as pharmaceuticals, and as natural insecticides or repellents. Despite diterpene ubiquity in plant systems, it remains unknown how plants control diterpene localization and transport. For many other small molecules, plant cells maintain transport proteins that control compound compartmentalization. However, for most diterpene compounds, specific transport proteins have not been identified, and so it has been hypothesized that diterpenes may cross biological membranes passively. Through molecular simulation, we study membrane transport for three complex diterpenes from among the many made by members of the Lamiaceae family to determine their permeability coefficient across plasma membrane models. To facilitate accurate simulation, the intermolecular interactions for leubethanol, abietic acid, and sclareol were parametrized through the standard CHARMM methodology for incorporation into molecular simulations. To evaluate the effect of membrane composition on permeability, we simulate the three diterpenes in two membrane models derived from sorghum and yeast lipidomics data. We track permeation events within our unbiased simulations, and compare implied permeation coefficients with those calculated from Replica Exchange Umbrella Sampling calculations using the inhomogeneous solubility diffusion model. The diterpenes are observed to permeate freely through these membranes, indicating that a transport protein may not be needed to export these small molecules from plant cells. Moreover, the permeability is observed to be greater for plant-like membrane compositions when compared against animal-like membrane models. Increased permeability for diterpene molecules in plant membranes suggest that plants have tailored their membranes to facilitate low-energy transport processes for signaling molecules.

Process of Zanthoxylum armatum DC. oil by a novel low-temperature continuous phase transition extraction: Evaluation of aroma, pungent compounds and quality

Zanthoxylum armatum DC. (ZAD) oil is one of the most popular seasonings for its unique flavor and numbing taste. However, aromatic and pungent components are easily affected by extraction process. In this study, a novel low-temperature continuous phase transition process (CPTP) was applied to extract ZAD oil, and the flavor and quality of oil were compared with the those of products obtained from fried process (FP), fresh squeezed process (FSP) and supercritical carbon dioxide process (SCDP) using Gas Chromatography-Mass Spectrometry/Olfactometry and UPLC-DAD-ESI-Q-TOF-MS/MS. The outcome demonstrated that the oil preserved higher volatile aroma components from SCDP and CPTP (606.073 mg/g and 194.905 mg/g, respectively). It's interesting to note that the majority of the alkyl components were only present in the ZAD oil created using CPTP, which further preserved the original makeup of the fragrance components of ZAD. Four ZAD oil had considerably different pungent compounds, with SCDP having the greatest concentration (16697.78 g/mL), followed by CPTP (6425.66 g/mL), FP (2662.6 g/mL), and FSP (719.69 g/mL). Physicochemical properties analysis indicated that unsaturated fatty acid content was highest for FSP oil (91.97%). Overall, as a fresh and effective extraction technique, CPTP may be appropriate for the extraction of natural fragrance components.

Diversity of volatile organic compounds in 14 rose cultivars

ABSTRACT This study illustrates the importance of multivariate analysis in profiling the levels of 104 volatiles across 14 modern rose cultivars obtained using headspace gas chromatography–mass spectrometry analysis. Despite differences in the abundance of volatiles, the cultivars ‘Lady Emma Hamilton’, ‘Golden Celebration’ and ‘The Poet’s Wife’ share similar profiles of volatile organic compounds (VOCs) and the absence of phenethyl alcohol, which is usually associated with a characteristic ‘rose’ fragrance. R. ‘Hansa’ is the cultivar with the greatest abundance of volatiles, with high levels of acyclic monoterpenes and aromatic compounds. No monoterpenes were detected from the climbing rose R. ‘August Gervais’. The study revealed that highly fragrant cultivars tend to produce VOCs in higher intensity and greater diversity. These findings also demonstrate the potential of using headspace analysis to compare the natural aromas of rose cultivars, which provides information to inspire innovation in the perfumery and food industries. Abbreviations AMT, acyclic monoterpenes; ARM, aromatic compounds; CMT, cyclic monoterpenes; GLV, green leaf volatiles; HEX, C6 lipid derivatives; OTH, other volatiles; PCoA, principal coordinates analysis; PEA, phenethyl alcohol; RI, retention index; SSQ, sesquiterpenes; TD-GC-MS, thermal desorption-gas chromatography-mass spectrometry; VOC, volatile organic compounds.

Construction of an Artificial Microbial Consortium for Green Production of (−)-Ambroxide

(−)-Ambroxide is a key ingredient of the fauna natural perfume ambergris, which is currently mainly supplied by the semi-synthetic route using plant-extracted sclareol as the raw material. Biosynthesis of (−)-ambradiol as the direct precursor of ambroxide holds promise for addressing the energy and environmental concerns in this semi-synthetic process. Due to the lack of the natural producer of ambradiol from glucose and the unrevealed metabolic pathway from sclareol to ambradiol, we divided the whole pathway into two modules and built an artificial microbial consortium using engineered sclareol-producing Saccharomyces cerevisiae and natural sclareol-converting Hyphozyma roseonigra ATCC 20624. S. cerevisiae strain YN42 was constructed by assembly and optimization of the plant-sourced sclareol synthetic pathway, producing 357.3 mg L–1 of sclareol in shake flasks. After validating the capability of ATCC 20624 in converting sclareol to ambradiol, an artificial microbial consortium was established for (−)-ambradiol production. In the optimized co-culture system, up to 644.2 mg L–1 of ambradiol was produced from glucose. By promoting the transmembrane transportation of sclareol via adding 2-hydroxypropyl-β-CD, a simplified induction-free co-culture system was constructed, producing 493.1 mg L–1 of ambradiol in shake flasks. This work provides an example for the biosynthesis of natural products with only a partially characterized pathway and points to a new production route of (−)-ambroxide.

Methylthioacetic acid, a derivative of aroma compounds from Cucumis melo var. conomon dose-dependently triggers differentiation and apoptosis of RCM-1 human colorectal cancer cells.

Methylthioacetic acid (MTA) is an acid-hydrolyzed derivative of a natural aroma compound, methylthioacetic acid ethyl ester isolated from Cucumis melo var. conomon (Katsura-uri, Japanese Picking Melon), and induces a villiform-like structure dome in RCM-1 human colorectal cancer cell culture. Thus far, the physiological and molecular properties of MTA-mediated dome formation remain unknown. Herein, MTA (not more than 2 mM) was demonstrated to differentiate the unorganized cell mass into the dome in RCM-1 cell culture by disclosing the correlation between dome formation and several intestinal differentiation markers such as alkaline phosphatase activity and the protein levels of dipeptidyl peptidase 4, villin, and Krüppel-like factor 4. Dome formation in RCM-1 cell culture was additively enhanced by the simultaneous administration of MTA and butyric acid (BA), suggesting that MTA directs the differentiation of RCM-1 cells, potentially through the same or similar pathway(s) shared with BA. Notably, a high dose of MTA (2 mM or more) elevated several apoptosis markers, such as DNA fragmentation, caspase-3/7 activity, and cleavage of poly(ADP-ribose) polymerase. Altogether, in addition to RCM-1 cell differentiation, MTA triggers apoptosis. These results indicate that MTA is a potential anticarcinogenic agent applicable in differentiation therapy and traditional chemotherapy against colorectal cancers.

CRISPR-Cas9-mediated editing of BADH2 gene triggered fragrance revolution in rice.

Fragrance is one of the most important quality traits for breeding in rice. The natural aroma substance 2-acetyl-1-pyrroline (2-AP) is a key fragrance compound among over 200 volatiles identified in fragrant rice. In addition to rice, there are other plant species that contain a germplasm that naturally produces a fragrant aroma. These other plant species all have lower activity levels of the enzyme BETAINE ALDEHYDE DEHYDROGENASE 2 (BADH2). Therefore, improving fragrance efficiency has been a focus of intensive research. Recent studies have engineered BADH2 gene, which is responsible for fragrance trait in non-fragrant cultivars of rice, using CRISPR-Cas9. Although engineering rice BADH2 can be useful for upregulating 2-AP, there are still a lot of restrictions on how it can be applied in practice. In this review article, we discuss the recent developments in BADH2 editing and propose potential future strategies to effectively target BADH2 for transcriptional regulation, with the goal of producing a better fragrance.

First evidence for multimodal animal seed dispersal in orchids

Identifying the mechanisms for seed dispersal and persistence of species is a central aim of ecology. Seed dispersal by animals is an essential form of dissemination in many plant communities, including seeds of over 66% of neotropical canopy tree species.1,2 Besides physical dispersal, animals influence seed germination probabilities through scarification, breaking dormancy, and preventing rotting, so plants often invest important resources in attracting them. Orchids are predominantly adapted to wind dispersal, having dust-like seeds that are easily uplifted. Exceptions include bird-,3,4 cricket-,5,6 and mammal-dispersed7 species, featuring fleshy fruits with hard seeds that germinate after passing the animal's digestive system. Given the similarity in fruit and seed morphology, zoochory has also been suggested in Vanilla,8,9,10,11,12,13,14,15 a pantropical genus of 118 species with vine-like growth.16,17,18 We test this prediction through in situ and ex situ experimentation using fruits of Vanilla planifolia, and wild relatives, from which vanillin-a widely used natural aroma and flavoring-is obtained. Seeds from dehiscent fruits are removed by male Euglossini collecting fragrances, a unique case in plants, and female Meliponini bees gathering nest-building materials, a first among monocots. By contrast, mammals, mostly rodents, consume the nutritious indehiscent fruits, passing the seeds up to 18h after consumption. Protocorm formation in digested and undigested seeds proves that scarification in the gut is not strictly required for germination. Multimodal seed dispersal mechanisms are proven for the first time in Orchidaceae, with ectozoochory and endozoochory playing crucial roles in the unusually broad distribution of Vanilla.

Formulation of Essential Oil-based Air Freshener Gel

Meanwhile, many reports of health risks caused by chemical or volatile organic compounds from gel air fresheners, odour removal products have been found, and their wide range of applications continues present in daily life. Therefore, the essential oil-based gel air freshener, a safe and available price product for the consumer and for human health protection, was studied and formulated with natural gelling agents (carrageenan, xanthan gum), natural fragrances (essential oils and aroma extracts), a surfactant (polysorbate 20), co-solvent (ethylene glycol), sodium benzoate, sodium chloride, and distilled water at varying concentrations. The essential oils extracted from pomelo peels, and aerial parts of lemongrass and peppermint acted both as flavoring agents and antimicrobial agents. Scent formulas of D-JA, D-PO, and D-FV blended from natural fragrances were conducted on the various gel formulas (I, II, and III). The most significant product samples were given by formula I (D-PO) being recorded at 0.0121 of specific gravity, a syneresis rate of 18.91%, and pH 4.88. This formula met the given criteria and panellists. Additionally, the formulas of air freshener gels containing essential oils as bioactive compounds presented antibacterial activity against Escherichia coli and Staphylococcus aureus., especially the formula with lemongrass essential oils and blended essential oils. These results indicated that essential oils on air freshener gel can inhibit the growth of bacteria and are safe for the consumer.

Industrial hydrophobic adsorbent screening for the separation of 1-phenylethanol and acetophenone

The biocatalytic conversion of acetophenone into 1-phenylethanol is a promising way to prepare a natural aroma. This work deals with adsorption on hydrophobic adsorbents as a way of separation of the product from the unreacted substrate. Seventeen types of industrial hydrophobic adsorbents were screened for their application potential in this separation. Batch equilibrium measurements were performed for these adsorbents, and the data were well fitted with the competitive Langmuir isotherm. All adsorbents had good capacities and many of them also exhibited good selectivity. The selectivity of the best was higher than 10. Since 1-phenylethanol was found to have a lower affinity than acetophenone, its recovery from the column outlet stream was first examined via ideal model simulations for a feed containing 5 g/L of 1-phenylethanol and 0.5 g/L of acetophenone. The adsorbent with the highest selectivity, Amberlite FPX68, could deliver a theoretical yield of 92.6 % for pure 1-phenylethanol. A fixed bed verification experiment provided a product collected in 55 bed volumes with 99 % purity and 87.8 % yield.

Bioconversion of isoeugenol to vanillin by bacillus firmus strain JAA1

Natural aromatic compounds are in growing demand in the food and pharmaceutical market. Due to limited sources for natural aroma compounds, there is an increasing interest in developing different sources for vanillin and another aromatic aldehydes. Some microbial species have been reported to produce aromatic aldehyde as an intermediate product in the degradation pathway of phenylpropanoids. The present work was carried out to isolate microbial species, capable of transforming isoeugenol to vanillin and its metabolites. Bacillus firmus strain JAA1 isolated from soil sample was found to transform isoeugenol into vanillin. The ability to produce vanillin from isoeugenol as substrate was screened through resting cells. The bacterial strain possessed the tolerance to grow in presence of isoeugenol as the sole carbon source. The bioconversion of isoeugenol to vanillin by the strain Bacillus firmus JAA1 was detected in the growing culture and its metabolite was detected through gas chromatography mass spectroscopy.

Identification of long non-coding RNAs involved in floral scent of Rosa hybrida.

Long non-coding RNAs (lncRNAs) were found to play important roles in transcriptional, post-transcriptional, and epigenetic gene regulation in various biological processes. However, lncRNAs and their regulatory roles remain poorly studied in horticultural plants. Rose is economically important not only for their wide use as garden and cut flowers but also as important sources of natural fragrance for perfume and cosmetics industry, but presently little was known about the regulatory mechanism of the floral scent production. In this paper, a RNA-Seq analysis with strand-specific libraries, was performed to rose flowers in different flowering stages. The scented variety ‘Tianmidemeng’ (Rosa hybrida) was used as plant material. A total of 13,957 lncRNAs were identified by mining the RNA-Seq data, including 10,887 annotated lncRNAs and 3070 novel lncRNAs. Among them, 10,075 lncRNAs were predicted to possess a total of 29,622 target genes, including 54 synthase genes and 24 transcription factors related to floral scent synthesis. 425 lncRNAs were differentially expressed during the flowering process, among which 19 were differentially expressed among all the three flowering stages. Using weighted correlation network analysis (WGCNA), we correlate the differentially-expressed lncRNAs to synthesis of individual floral scent compounds. Furthermore, regulatory function of one of candidate lncRNAs for floral scent synthesis was verified using VIGS method in the rose. In this study, we were able to show that lncRNAs may play important roles in floral scent production in the rose. This study also improves our understanding of how plants regulate their secondary metabolism by lncRNAs.

Characterization of Odorants in White Leaf Mountain Mint, Pycnanthemum albescens.

White leaf mountain mint, Pycnanthemum albescens Torrey & A. Gray, also known as white mountain mint or white leaved mountain mint, is a species endemic in the American Southeast. In the present study, 24 odorants were identified using solvent-assisted flavor evaporation, aroma extract dilution analysis, and gas chromatography-mass spectrometry. Nine odorants with flavor dilution factors ≥16 were quantitated by stable isotope dilution assays, and odor activity values (OAVs) were calculated. In addition, the enantiomeric proportions of several chiral odorants were determined by chiral chromatography. Odorants with OAV ≥1 included 1,8-cineole (eucalyptus; OAV 9200), myrcene (terpeny; OAV 1400), linalool (floral, citrus; OAV 370), β-ionone (floral, violet; OAV 64), borneol (earthy; OAV 55), bornyl acetate (earthy, fruity; OAV 19), and eugenol (clove; OAV 3.1). Odor simulation experiments revealed that a mixture of the odorants with OAV ≥1 successfully mimicked the odor of an aqueous extract of the plant when combined in their natural concentrations. This study lays the groundwork for future studies aimed at determining the natural aroma variation within different populations of P. albescens and aids in the future development of selections and hybrids with targeted aroma profiles of commercial interest.

Comprehensive analysis of bZIP gene family and function of RcbZIP17 on Botrytis resistance in rose (Rosa chinensis)

Rose is an important ornamental plant and a commercial source of natural fragrances and essential oils. However, gray mold is the most serious disease of rose during cultivation and post-harvest. In plants, the bZIP gene family is one of the important families of transcription factors that regulate many biological processes, including pathogen defense, light response, and stress signaling. Although there were reports that bZIPs play a key role in plant pathogen defense, there have been no comprehensive studies or functional analysis of the bZIP gene family in rose (Rosa sp.) yet. In this study, a genome-wide analysis of the bZIP family gene (RcbZIPs) in rose was carried out to investigate their phylogenetic relationships, gene structure, chromosome localizations, collinearity, and the function of RcbZIP17. In the rose genome, 48 RcbZIP genes were identified and divided into 11 subgroups by phylogenetic relationships. Moreover, gene structure analysis agreed with phylogenetic tree grouping. The RcbZIPs were unevenly distributed on all seven chromosomes. The gene duplication events indicated that segmental duplication of the entire genome may play a key role in gene duplication. A Ka/Ks analysis showed that they mainly underwent purifying selection with limited functional differentiation. Gene expression analysis showed that 16 RcbZIPs were significantly differential expressed by Botrytis cinerea infection. It is speculated that these RcbZIPs are candidate genes for regulating the response of rose to gray mold infection. According to virus-induced gene silencing (VIGS) and overexpression (OE) studies, RcbZIP17 was implicated in the resistance of rose to gray mold. This study provides useful information for further study on the function of the bZIP gene family in rose.

Jelly candy enriched with BRS Violeta grape juice: Anthocyanin retention and sensory evaluation

This study aimed to evaluate the applicability of grape juice, despite being from immature grape, as a bioingredient and natural coloring in jelly candy. The product was made containing BRS Violeta grape juice, gelatin, honey, agar and natural aroma. The qualitative and quantitative composition of anthocyanins present in the grapes, the juice produced, and the juice-containing jelly candy were determined using HPLC-DAD- ESI -MS/MSn; and, sensory analysis methods (acceptance and Rate-All-That-Apply (RATA)) were used to assess the quality of the candy produced. The product had a concentration of total anthocyanins (mg malvidin-3,5-glucoside·kg fruit−1) of 686.78, with a retention of 41% in relation to the concentration determined in the juice. However, compared to the grape, the concentration of diglycosylated anthocyanins, including the coumarylated ones, increased because they are more stable compounds. According to the sensory characterization, the candy presented a satisfactory acceptance; according to the RATA method, it was characterized mainly as purple and uniform in color, shiny and gelatinous in appearance. Finally, it is concluded that the application of juice, combined with the other ingredients of the formulation, resulted in a product pleasant and with the considerable concentration of anthocyanins and, with grape odor and flavor.