Natural Color Research Articles

The Effect Of Substituting Butterfly Pea Extract As A Natural Dye In Mochi Production

Mochi is a traditional Japanese food made from glutinous rice flour with a sweet taste and a distinctive soft texture that turns chewy when eaten. Mochi is typically round in shape, filled with peanut paste, and coated with roasted cornstarch. In Indonesia, mochi has various variations, one of which involves modifying the mochi's outer layer by adding food coloring to the dough. As time progresses, people have become more aware of the negative effects of synthetic ingredients, leading consumers to shift towards using natural food colorings. Butterfly pea flowers are one alternative source of natural food coloring. These flowers contain anthocyanin compounds that can produce a blue color. The use of butterfly pea flowers does not affect the taste of food products since it only contains anthocyanins. The purpose of this study is to analyze the quality of mochi with the addition of butterfly pea flower extract. The research method employed is a pure experiment, using a Completely Randomized Design (CRD) with three repetitions. Data was collected by surveying a limited group of panelists, who are Family Welfare Science lecturers with a concentration in Culinary Arts at the Faculty of Tourism and Hospitality, Universitas Negeri Padang. Data was analyzed using Analysis of Variance (ANOVA), and if F-value > F-table, then the Duncan test was conducted. The research results indicate a significant effect on color quality. However, the shape (neat and uniform), aroma, texture, and taste did not show a significant effect. The highest overall scores were as follows: neat shape 3.11 (X1), uniform shape 3.11 (X1), blue color 3.44 (X1), pleasant aroma 3.33 (X0), chewy texture 3.67 (X1), and sweet taste 3.33 (X1). The best result in this study was treatment (X1) with a 50% substitution of butterfly pea flower extract.

The Influence Of Corn Flour Substitution On The Quality Of Dry Noodles

The use of corn flour contains various essential components such as a source of plant-based vitamin A, also known as beta-carotene content, essential fatty acids, protein, carbohydrates, and high dietary fiber. However, the utilization of local corn flour is not maximized in the food industry, as it is mainly focused on supplying animal feed, even though corn is one of Indonesia's staple commodities in supporting the national food security program. The use of corn flour can replace synthetic coloring agents with a natural yellow color obtained from the use of local corn flour derived from the beta-carotene pigment contained in corn. This research aims to analyze the influence of substituting corn flour by 0% (X0), 20% (X1), and 40% (X2) on the quality of dry noodles in terms of color, aroma, texture, and taste. The research design used was a pure experiment with a Completely Randomized Design (CRD) method. The data used were obtained directly from three limited panelists using the organoleptic evaluation format for the quality of dry noodles with corn flour substitution. The collected data were then tabulated in table format, and an Analysis of Variance (ANOVA) was conducted. If the analysis of variance shows Fcount > Ftable, then Duncan's test was carried out. The results of the research indicate a significant influence of corn flour substitution at 0% (X0), 20% (X1), and 40% (X2) on the quality of color (pale yellow), and texture (chewy). The best result was observed at the 20% substitution level (X1) with a 20% substitution of corn flour from the total amount of wheat flour used.

A water-based ultrasound-assisted extraction system to obtain natural blue colorant from Genipap (Genipa americana L.)

This study aimed to optimize the extraction of a blue colorant using ultrasound-assisted technology employing water as the solvent from genipap, an Amazon fruit. Process conditions were optimized by multiresponse analysis through the antioxidant activity by the ferric reducing antioxidant potential (FRAP) assay, the total phenolic content, and the b* and C* color parameters. The optimized conditions of 70 °C, 325 W, solid: solvent ratio of 1:40 (m/v), and time of 10 min were validated externally at a level of 90 % confidence. The composition of aqueous blue extract obtained under the greatest conditions was evaluated by ultra-performance liquid chromatography (UPLC). Besides, in vitro bioactivities such as digestive enzymatic inhibitions assays, and antiproliferative activity through human cells assays were evaluated. Genipin, geniposide, and other ten iridoids were identified in the aqueous blue extract. The natural colorant obtained presented the capacity of inhibiting enzymes involved in lipid and glucose digestion, suggesting a potential control of obesity and glucose levels in blood without showing cytotoxicity. Therefore, this study presented, for the first time, the optimal operational conditions to obtain a natural blue colorant from genipap in a green approach with interesting biological properties for the food and pharmaceutical industries.

Dialogue on Artistic Expression: A Study of Oriental Elements in 19th-Century Impressionism

As one of the most significant art movements in the 19th century, Impressionism broke down conventional boundaries, laying the foundation for today’s artistic styles to flourish. This paper aims to explore the integration of Oriental elements of Impressionism and to reveal the influence of cultural exchange on art movements. The introduction section provides a comprehensive overview of Impressionism, focusing on the research goals that revolve around Oriental aspects. The paper utilises an exhaustive approach, including a literature review, artwork analysis, and examination of historical records, ensuring a thorough exploration of Oriental influences. The structure of this paper acts as a guide for readers, highlighting unique contributions such as exploring little-known Eastern elements and interpreting artworks from new perspectives. By deeply studying the historical background of Impressionism, this paper discusses the interests in Eastern art and its influences on the Western art movement in the 19th century, examines the channels of cultural exchange, and analyses the reception of Oriental elements through case studies. At the same time, the influences of oriental traditions and elements such as brushwork, composition, and calligraphy on the natural depiction, colour application, daily life, symbolism, and character representation of Impressionist paintings are also discussed. In conclusion, this paper summarises the key findings and contributions and emphasises the importance of Oriental elements in Impressionist art.

The Reconstruction of Indigenous Science into Scientific Knowledge in the Natural Color Process from Lurik Klaten

Teaching science in schools rarely includes local wisdom. Local wisdom-based learning is more suitable for the regional characteristics and students’ daily life experiences and also supports sustainability and cultural diversity. The reconstruction of indigenous science into scientific knowledge will show the part of local wisdom that is suitable for science learning. This research aimed to reconstruct indigenous science into scientific knowledge from the process of natural color that was conducted in the community of Lurik Klaten in Pedan and Cawas Subdistrict, Klaten Regency, Central Java, Indonesia. The outcome of this research was scientific knowledge from the result of indigenous science reconstruction that was based on society’s culture about the process of natural color. The expected benefit of this research was to be the contextual educational resource for teachers of science in the school. The method of this research was qualitative descriptive through visceral interviews, direct observation and document study about the traditional process of natural color. The research focused on natural color production, especially on knowledge of traditional lurik craftsman related to the raw materials of natural color and also the process of coloring lurik. The obtained data were analyzed, verified, and constructed into scientific knowledge and were interpreted to get meaningful information. The conclusion was that there was five indigenous science from natural color production which could be reconstructed into scientific knowledge and also appropriate with several competences in science education. Keywords: Indigenous Science, Natural Color Process, Lurik Klaten

High Fidelity Full-Color Optical Sectioning Structured Illumination Microscopy by Fourier Domain Based Reconstruction

The natural color of biological specimens plays a crucial role in body protection, signaling, physiological adaptations, etc. Full-color optical sectioning structured illumination microscopy (OS-SIM) color is a promising approach that can reconstruct biological specimens in three-dimension meanwhile maintaining their natural color. Full-color OS-SIM takes the advantages of rapid imaging speed, compatibility with fluorescence and non-fluorescence samples, compact configuration, and low cost. However, the commonly used HSV-RMS reconstruction algorithm for full-color OS-SIM faces two issues to be improved. One is the RMS (root-mean-square) OS reconstruction algorithm is prone to background noise, and the other is the reconstruction is bound in RGB and HSV color spaces, consuming more reconstructing time. In this paper, we propose a full-color Fourier-OS-SIM method that allows for the OS reconstruction using the high-frequency spectrum of the sample and thus is immune to the low-frequency background noise. The full-color Fourier-OS-SIM directly runs in the RGB color space, providing an easy way to restore the color information. Simulation and experiments with various samples (pollen grains and tiny animals) demonstrate that the full-color Fourier-OS-SIM method is superior to the HSV-RMS method regarding background noise suppression. Moreover, benefiting from the background noise suppression merit, the quantitative morphological height map analysis with the full-color Fourier-OS-SIM method is more accurate. The proposed full-color Fourier-OS-SIM method is expected to find broad applications in biological and industrial fields where the 3D morphology and the color information of objects both need to be recovered.

Clinical evaluation of therapeutic efficacy of a Siddha herbo mineral formulation “Kumaara Veeriya Kaantha Chenduram’’ with “Nellikkai Legiyam” (internal) in the treatment of Iron Deficiency Anemia

Abstract: Background: Kumaara veeriya kaantha chenduram (KVKC) is a siddha herbomineral formulation indicated for paandunoi in Siddha literature (1).Paandu noi is a disease characterized by changes in the body's natural color andpallor found in the skin, nails and conjunctiva. Objectives: To determine the efficacy of the KVKC, the open labed clinical study was conducted in National Institute of Siddha OPD. Materials and Methods: The 30 patients with Iron deficiency anaemiawere selected and treated with KVKC orally with the dose of 260 milli gram twice in a day with Nellikailegiyam6 gram continuously for 45 days. Laboratory investigations for hemoglobin was done on 1st day and every 15 days once of the study. Results: Clinical assessment was done, based on the before and after treatment scores which was compared statistically and recorded.Regarding Hemoglobin level, 24(80%) patients showed increase of 1 to 3 grams from base level, 6(20%) patients showed increase of 0.1 to 0.9 grams from base level.Regarding Serum Ferritin, out of 30 patients, 9 (30%) patients showed 0.1-3.5 ng/ml increase from its base level, 21 (70%) patients showed 3.6-7.0 ng/ml increase from its base level.From the Statistical studies, the Mean ± Standard deviation for Haemoglobin before treatment was 9.7 ± 0.93 and after treatment was 11.18 ± 1.08 which was statistically highly significant (p<0.0001).And Serum ferritin before treatment was 9.27 ± 3.31, after treatment was 13.5 ± 3.49 which was statistically significant (p<0.0001).

The road less taken: Dihydroflavonol 4-reductase inactivation and delphinidin anthocyanin loss underpins a natural intraspecific flower colour variation.

Visual cues are of critical importance for the attraction of animal pollinators, however, little is known about the molecular mechanisms underpinning intraspecific floral colour variation. Here, we combined comparative spectral analysis, targeted metabolite profiling, multi-tissue transcriptomics, differential gene expression, sequence analysis and functional analysis to investigate a bee-pollinated orchid species, Glossodia major with common purple- and infrequent white-flowered morphs. We found uncommon and previously unreported delphinidin-based anthocyanins responsible for the conspicuous and pollinator-perceivable colour of the purple morph and three genetic changes underpinning the loss of colour in the white morph - (1) a loss-of-function (LOF; frameshift) mutation affecting dihydroflavonol 4-reductase (DFR1) coding sequence due to a unique 4-bp insertion, (2) specific downregulation of functional DFR1 expression and (3) the unexpected discovery of chimeric Gypsy transposable element (TE)-gene (DFR) transcripts with potential consequences to the genomic stability and post-transcriptional or epigenetic regulation of DFR. This is one of few known cases where regulatory changes and LOF mutation in an anthocyanin structural gene, rather than transcription factors, are important. Furthermore, if TEs prove to be a frequent source of mutation, the interplay between environmental stress-induced TE evolution and pollinator-mediated selection for adaptive colour variation may be an overlooked mechanism maintaining floral colour polymorphism in nature.

Ecological dyeing and UV-protective functionalization of cotton/lyocell blend fabrics designed for high comfort summer clothing

Wearing clothes that absorb or block harmful UV radiation is one of the most effective forms of protection against sun damage and skin cancer. In the current study, sustainable processes which will provide high clothing comfort and a sufficient level of UV protection for fabrics used in production of lightweight sun-protective summer clothing were proposed. In accordance with the scope of the study, structural properties, low-stress mechanical properties, surface characteristics, permeability, and UV-protection properties of three woven fabrics produced with different weft settings and lyocell ratios were determined. The fabric type which had the most desirable results was selected for dyeing experiments. Avocado (Persea americana) seed was used as an eco-friendly source for dyeing and UV-protective functionalization of cotton/lyocell blend fabric. To obtain different hues, the fabrics were mordanted according to two different recipes using magnesium sulphate and ferrous sulphate and dyed with the natural colorant extracted from avocado seeds. Comfort, color, fastness, and UV-protective properties of dyed samples were evaluated by laboratory tests. Results indicated that it is possible to achieve an excellent level of UV protection (UPF 50+) by dyeing cotton/lyocell blend fabrics with avocado seed extract and produce summer clothing with improved comfort and UV-protective properties, without using any toxic materials.

Nurturing Natural Beauty: Empowering MAN 1 Model Bengkulu's Female Students with Red Dragon Fruit (Hylocereus polyrhizus) Cosmetics Crafting Workshop

Introduction: The use of lipstick, blush-on, and various colored cosmetic products is highly popular among teenage girls. However, the adverse effects of hazardous colorants in cosmetics include respiratory irritations, skin irritations, eye injuries, gastrointestinal toxicity, and carcinogenic properties. Red Dragon Fruit contains a significant amount of red anthocyanin antioxidants, making it a viable natural colorant for food, cosmetics, and textiles. This community engagement project aimed to enhance the understanding of teenage girls, especially students of MAN 1 Model City of Bengkulu, regarding illegal cosmetics containing harmful and prohibited ingredients. It also aimed to improve the girls' skills in crafting natural Lip Tint and Blush-On preparations from Red Dragon Fruit (Hylocereus Polyrhizus), promoting a return to nature in cosmetic choices. This initiative was expected to inspire creative ideas among teenagers for designing alternative economic activities centered on producing natural and halal cosmetics made from herbal ingredients. Methods: The project involved educational and awareness-raising activities about the dangers of forbidden cosmetic ingredients, followed by workshops on making Lip Tint and Blush-On formulations. These workshops were led by faculty members from the School of Medicine and the School of Pharmacy, with assistance from students of the Faculty of Mathematics and Natural Sciences at the University of Bengkulu. Results: Evaluation results showed that workshop participants experienced a 63.2% increased in knowledge and gained the ability to independently create Lip Tint and Blush-On products from red dragon fruit. Conclusion: The workshop successfully produced natural Lip Tint and Blush On and the project also heightened the students' interest in the Pharmacy Program.

Suitability of dyes from cinnamon bark on wool fibers using metal and bio-mordants.

Growing concern about the environmental effects of metal mordants and synthetic dyes is encouraging the use of bio-mordants and natural colorants. Cinnamon bark is a rich source of natural colorants such as cinnamaldehyde and tannins. The main purpose of this research was to study and compare the effect of bio-mordants versus metal mordants in terms of colorimetric parameters and color fastness properties of cinnamon bark on wool fibers. Accordingly, some bio-mordants, including date kernel, peppermint, banana peel, and artemisia, as well as some metal mordants like aluminum potassium sulphate and copper sulphate, were studied based on three conventional mordanting methods (pre-, meta-, and post-mordanting). The results indicated that the conjunction of metal mordants and polyphenolic bio-mordants with cinnamon colorants can create different hues and tones of brown. Also, the color produced by cinnamon in wool fibers has poor color fastness and low color strength. Overall, bio-mordants have presented good color properties, making the dyeing process eco-friendly and greener. Among the applied bio-mordants, peppermint has created the best color strength and color fastness.

One‐Step Photo‐Patterning Process for Multi‐Modal and Transformative Structural Coloration: Toward Anti‐Counterfeiting Applications

AbstractThe selective interactions of light with matter enable the existence of colors in nature. Through the precise modification of material properties, photonic crystals, plasmonic nanoparticles, and dielectric metasurfaces can produce bright and vivid structural colors. However, this approach is rendered inadequate by its complex fabrication processes, limited working area, and lack of inherent material‐property tuning. Here, a novel one‐step fabrication process based on ultraviolet‐curable chitosan (UVCC) for generating structural multicolor patterns in polydimethylsiloxane in a single bending event is presented. UV exposure through a grayscale photomask controls the polymerization of the UVCC, enabling manipulation of its thickness, thereby allowing the production of size‐, wavelength‐, and amplitude‐controllable wrinkles under compressive stress. This enables the exhibition of selective structural coloration in response to incoming incident light. Upon the release of applied stresses, the wrinkles disappear, and the bilayer device becomes transparent again. Notably, repeating the process allows for dynamic covert–overt switching of the structural colors. This firm control over the active‐layer thickness, simple device structure, cost‐effectiveness, facile fabrication process, and vibrant‐color tuning ability make this one‐step photopatterning approach more competitive for structural‐color applications. Finally, the authenticity of a cosmetic cream is evaluated to demonstrate the anti‐counterfeiting effect of the UVCC circumference wrinkles.

Characterization and Discrimination of Marigold Oleoresin from Different Origins Based on UPLC-QTOF-MS Combined Molecular Networking and Multivariate Statistical Analysis.

Marigold oleoresin is an oil-soluble natural colorant mainly extracted from marigold flowers. Xinjiang of China, India, and Zambia of Africa are the three main production areas of marigold flowers. Therefore, this study utilized ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technology, combined with Global Natural Products Social Molecular Networking (GNPS) and multivariate statistical analysis, for the qualitative and discriminant analysis of marigold oleoresin obtained from three different regions. Firstly, 83 compounds were identified in these marigold oleoresin samples. Furthermore, the results of a principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) indicated significant differences in the chemical compositions of the marigold oleoresin samples from different regions. Finally, 12, 23, and 38 differential metabolites were, respectively, identified by comparing the marigold oleoresin from Africa with Xinjiang, Africa with India, and Xinjiang with India. In summary, these results can be used to distinguish marigold oleoresin samples from different regions, laying a solid foundation for further quality control and providing a theoretical basis for assessing its safety and nutritional aspects.

Bio-inspired fabrication of non-iridescent structural color coatings with excellent colorfastness for rapid and large-scale colorization of textile

Structural color in nature provides a new strategy for eco-friendly colorization. Biomimetic construction of amorphous photonic crystals (APCs) on the substrate surface can form a specific non-iridescent structural color, which can be widely used in textile, paint and other fields. However, the APCs structural color has the shortcomings of dim color and poor colorfastness, which restrict the industrial application. In this study, base on the structural design of APCs building blocks, a novel copolymer containing reactive epoxy groups of poly (styrene-co-glycidyl methacrylate) (P(St-co-GMA)) was synthesized, which was used for fabricating APCs structural color by rapid spray coating method. P(St-co-GMA) nanospheres with different particle diameters can be self-assembled into APCs showing different crack-free structural colors. This research reports for the first time that the polymer nanospheres with two different particle diameters were synthesized by one-pot polymerization to enhance the color brightness of structural color. P(St-co-GMA) nanospheres structural colorized fabrics showed excellent colorfastness, flexibility and air permeability. This research promotes the industrial application of structural color for the rapid larger-scale fabrication of APCs with excellent colorfastness on substrates through the synthesis of polymer nanospheres with reactive epoxy groups.

Recent advances on pH-responsive polymers integrated with nature colorants: from preparation to applications

This review covers the progress in recent years on pH-responsive polymers integrated with natural colorants. The classification of various natural colorants, including anthocyanin, curcumin, and alizarin, and the pigments’ structural changes at different pH are introduced. Subsequently, the latest research in substrates for immobilizing natural colorants is discussed. Moreover, their applications on wound dressing for bacterial infections monitoring, smart package for food quality monitoring, and textile dyeing, are outlined. Finally, this study was summarized by emphasizing the current challenges and future opportunities in the field of pH-responsive polymers.

Biopolymer-based intelligent packaging integrated with natural colourimetric sensors for food safety and sustainability.

Increasing concerns about global food safety and security demands innovative solutions, particularly in food packaging technologies. This review paper investigates the advanced integration of natural colourimetric sensors with biopolymer-based packaging materials, with a focus on developments over the past 5 years. These sensors change colour in response to environmental stimuli such as oxygen, temperature, pH and relative humidity, intuitively indicating food freshness and safety. The paper emphasizes the recent advancements in using natural colourants, such as alizarin, anthocyanins, betacyanins, chlorophyll, curcumin and shikonin. When combined with either natural or synthetic biopolymers, these colourants contribute to a sustainable and eco-friendly approach to food packaging. Such technological advances could notably decrease the incidence of foodborne illnesses by signaling potential spoilage or contamination, while also addressing food wastage by providing clear indications of edibility. Although challenges remain in sensor longevity and widespread adoption, the prospects for biopolymer-based food packaging with embedded natural colourimetric sensors are promising.

Assessing the potential of C-phycocyanin as a natural colorant for non-alcoholic carbonated beverages

The multibillion-dollar carbonated beverage industry is currently facing questions from health-conscious consumers over negative health effects of such beverages. Decreasing consumption trends have forced companies to look for healthier choices for their products. C-phycocyanin CPC, a bright blue cyanobacterial pigment with anti-oxidant and other health benefits has been proposed as a candidate in edible drinks. We found that CPC is stable in a wide pH and temperature regime. Reaction kinetics for 12 weeks at 4 °C in non-alcoholic carbonated beverages (B1-B4) showed that B3 (sweetened, ~30 % degradation) best preserved CPC integrity while B1 (non-sweetened, ~87 % degradation) was ineffective. Other beverages (sweetened) could preserve ~ 49 % CPC integrity. Behnajady-Modirshahla-Ghanbary and first order kinetic models explained CPC degradation with and without preservative (sucrose), respectively. The ’consume-by’ times suggest possible refrigeration from ~ 13 hours to 27 days for various CPC-containing beverages. Results suggest CPC could be filter-sterilized and added to non-alcoholic beverages before being packaged in cans or tetra packs to avoid light exposure.Graphical abstract

Automatic Marker Generation to Similarity Based Region Merging Algorithm using Edge Information

One of the most important tasks in computer vision is image segmentation. Several interactive strategies are utilized for picture segmentation because automated techniques are difficult for this kind of work. The outcome of interactive methods is mostly determined by user input. Obtaining excellent interactions for huge datasets is challenging. However, automated picture segmentation is starting to play a significant role in image analysis and computer vision. Effective and efficient segmentation outcomes are obtained using the interacting region merging method suggested by Maximal Similarity based region merging algorithm. Limitation of MSBRM is it requires some efforts on the part of users and is yet not a fully-automatic approach. Here, we suggest a completely fresh unsupervised image segmentation method that combines edge information with MSBRM. We propose an integrated framework to generate object markers for similarity based region algorithm using edge information. Long edges give rough distribution of objects in image. After retrieving edges using phase congruency, edge processing operations are employed to remove small edges and to group color similar long boundaries. Centroids of long boundaries are used as object markers to the MSBRM algorithm. The generation of object markers is done using edge segment grouping. These object markers guide the region merging process. The proposed method shows its effectiveness in segmenting natural real world color images.

The Natural Way of Food Colouring

Color is a measure of quality and nutrient content of foods. The objective of adding color to foods is to make them appealing, ent the loss of color during processing, to improve the quality and also to influence the consumer to buy a product. At augment present, the demand for natural dyes is increasing worldwide due to the increased awareness on therapeutic and medicinal properties and their benefits among public and also because of the recognized profound toxicity of synthetic colors. Natural dyes are those derived from naturally occurring sources such as plants, insects, animals and minerals. Among all the natural dyes, plant-based pigments have medicinal values so are mostly preferred. Today the food industry and color suppliers are however constantly motivated to work towards the improvement of the technical and physical properties of the color preparations. Development of cost-effective, viable technology for the preparation of a food color and its application in foods is a challenge and the need of the day. This review article covers recent developments in technological advances of food colors with respect to natural color application and stability in foods compared to synthetic colors and the detail basic chemical information about of the major pigments.

Advancement in extraction and characterization techniques of natural dyes from dye yielding plants sources: a review

Purpose Everyone is extremely concerned about environmental protection and health safety due to the rise in living standards. Plant-derived natural dyes have garnered much industrial attention in food, pharmaceutical, textile, cosmetics, etc. owing to their health and environmental benefits. The present study aims to focus on the elimination of the use of synthetic dyes and provides brief information about natural dyes, their sources, extraction procedures with characterization and various advantages and disadvantages. Design/methodology/approach In producing natural colors, extraction and purification are essential steps. Various conventional methods used till date have a low yield, as these consume a lot of solvent volume, time, labor and energy or may destroy the coloring behavior of the actual molecules. The establishment of proper characterization and certification protocols for natural dyes would improve the yielding of natural dyes and benefit both producers and users. Findings However, scientists have found modern extraction methods to obtain maximum color yield. They are also modifying the fabric surface to appraise its uptake behavior of color. Various extraction techniques such as solvent, aqueous, enzymatic and fermentation and extraction with microwave or ultrasonic energy, supercritical fluid extraction and alkaline or acid extraction are currently available for these natural dyes and are summarized in the present review article. Originality/value If natural dye availability can be increased by the different extraction measures and the cost of purified dyes can be brought down with a proper certification mechanism, there is a wide scope for the adoption of these dyes by small-scale dyeing units.