Background:This study aims to assess the effectiveness of self -instructional - module on knowledge regarding natural birthing methods among antenatal mothers of selected hospitals. It is quasi experimental research. Purpose of the study was to know the pre assessment knowledge regarding natural birthing methods followed by the intervention, which included administration of self-instruction-module, which was based on the content related to natural birthing methods. After administration post evaluation of the knowledge score was done to compare the effectiveness of the intervention.
 Material and method: A quasi experimental research design was used while the approach utilized was quantitative. Pre-test- post-test control group research design was used for the study. The study was done at tertiary hospital of a cosmopolitan city, while the samples were antenatal mothers who were attending the OPD’s. Data collection tool used was a structured questionnaire on natural birthing methods which included demographic questions and knowledge based questions. Analysis was done by using descriptive inferential statistics.
 Result:Results revealed that experimental group knowledge score in pre-test was 9.2 as compared to 11.2 in the post-test for the p-value of 0.05. In control group, knowledge score in pre-test was 8 as compared to 8.5 in the post-test. Association was found with the demographic variable of education
 Conclusion: Average mean difference in knowledge score in antenatal mothers in experimental group was significantly higher than that in control group hence It is evident that the self-instructional module is significantly effective in improving the knowledge among antenatal mothers regarding natural birthing methods.