Melissopalynological analysis of 39 honey samples from Oaxaca, Mexico, enabled us to establish the important plant sources exploited by bees during the principal harvest in four districts of the State of Oaxaca, Mexico. A total of 64 taxa belonging to 29 families were recorded. These subtropical honeys were characterised by their botanical origin as follows: (a) monofloral honeys of Bursera simaruba, Clethra mexicana, Cordia alliodora, Lonchocarpus sp., Mangifera indica, Miconia argentea, Orbignya cohune and Quercus sp.; (b) bifloral honeys with an association of Heliocarpus donnell-smithii and Ceiba sp., Lonchocarpus sp. and Mimosa pudica, H. donnell-smithii and Mangifera indica, Miconia argentea and Miconia tenuiflora; (c) oligofloral honeys of Asteraceae; and (d) multifloral honeys with three or four species ≥10%. Monofloral honeys were placed in classes I, II, III, IV and V. Oligofloral were class II, bifloral were classes I and II, and polyfloral honeys were assigned to classes I, II and III. Honey samples of Apis mellifera had a diversity index range of 0.3 to 2.7. It is well known that this bee is polylectic and has a heterogeneous foraging behaviour. In the State of Oaxaca, it prefers resources of secondary vegetation from low deciduous forest, although taxa of economic importance were also utilised, for instance, Mangifera indica and Citrus sinensis. Oaxaca has important and diverse native resources, and beekeeping activity needs to be promoted because of its potential to develop new types of honey.
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