National Forest Research Articles

Response of Soil CO2 Emission to Addition of Biochar and Dissolved Organic Carbon along a Vegetation Restoration Gradient of Subtropical China

Biochar, as a soil amendment, has been widely confirmed to increase soil carbon sequestration. However, how biochar addition affects soil carbon changes during the vegetation restoration process is still unclear, which constrains our ability to explore biochar’s application in the technology of soil carbon sequestration in forests. We conducted an incubation experiment on biochar and dissolved organic matter (DOM) addition to soil at three stages of revegetation (degraded land (DS), plantation forest (PS), and secondary natural forest (NS) in Changting County in Fujian province, China) to investigate the effects of vegetation restoration, biochar, DOM, and their interaction on soil CO2 emission and its relative mechanisms. We found that the accumulative release of CO2-C in the NS and PS soils was 7.6 and 6.8 times higher, respectively, in comparison to that from the DS soil. In the DS, biochar addition significantly increased the accumulative release of CO2-C, soil pH, NH4+-N content, qCO2, phenol oxidase, and peroxidase activities. Peroxidase activities were positively correlated with the accumulative release of CO2-C, and oxidase was the most important direct factor influencing the accumulative release of CO2-C in the DS. However, the accumulative release of CO2-C, soil NH4+-N content, qCO2, β-glucosidase, and N-acetylglucosaminidase activities was significantly reduced after the application of biochar in the PS and NS. These two hydrolases were positively associated with the accumulative release of CO2-C, and hydrolase was the most vital direct factor influencing the accumulative release of CO2-C from the PS and NS soils. The positive effect of DOM addition on CO2 emission under biochar application declined with a vegetation restoration age increase. Our results indicated that biochar could alter microbial physiological processes, inhibit qCO2 and hydrolase activities, and further decrease CO2 emission in relatively fertile soil from the PS and NS; but in the relatively barren soil from the DS, biochar might promote CO2 emission by stimulating microorganisms to enhance qCO2 and oxidase activities.

Plantation forestry of Alder-leaf Birch (Betula alnoides) affects composition but not interactions of mixed-species bird flocks in southwestern China

Human disturbance impacts mixed-species bird flocks (“flocks”). Unfortunately, the impact on flocks by one large-scale disturbance, plantation forestry, has remained little explored. We examined how plantation forestry of a widespread yet understudied timber species, Alder-leaf Birch (Betula alnoides, “birch”), affects the composition and interactions of flocks in the Indo-Burma biodiversity hotspot in southwestern China. We conducted transect surveys to sample flocks in birch plantations and natural forests of two age classes (mature and young). While flock size and species richness per flock were similar across land-use types, rarefied species richness accounting for unequal sampling effort was noticeably higher in mature forests. Furthermore, flock composition differed across land-use types, with differences related to species' morphological characteristics, dietary preferences, and foraging substrates. Specifically, mature forests supported flock participants with large bodies, poor dispersal ability, and a fruit-eating diet. Birch plantations offered equal support to invertivores as both natural forests, and additional support to bark foragers. Lastly, interactions among flocking species quantified by social network metrics were similar across land-use types, suggesting that birch plantations perserved the flocking behavior itself. Our study reveals the conservation potential of birch plantations in supporting invertivorous birds and preserving interactions in flocks. More importantly, it highlights the irreplaceability of mature forests because of their unique species composition. We recommend promoting birch planting without compromising local economies and protecting remnant mature forests through education programs and continued research.

Beware of the stripes: Encounter between Lycalopex griseus and Conepatus chinga in their natural environment in central Chile

We document an occasional encounter between carnivore species Lycalopex griseus and Conepatus chinga, using camera traps in native Mediterranean forest of the Coast Range in central Chile. Photographic evidence is provided as to the agonistic behavior exhibited by C. chinga and the reaction of L. griseus to this interaction. In view of incomplete niche segregation and the spatiotemporal overlap in the habitat use for both species, our observations contribute to a better understanding of the interactions and ecological dynamics of the carnivore community of this ecosystem.

Satellite Image Fusion Airborne LiDAR Point-Clouds-Driven Machine Learning Modeling to Predict the Carbon Stock of Typical Subtropical Plantation in China

In the current context of carbon neutrality, afforestation is an effective means of absorbing carbon dioxide. Stock can be used not only as an economic value index of forest wood resources but also as an important index of biomass and carbon storage estimation in forest emission reduction project evaluation. In this paper, we propose a data-driven machine learning framework and method for predicting plantation stock based on airborne LiDAR + satellite remote sensing, and carried out experimental verification at the site of the National Forest emission reduction project in Southern China. We used step-up regression and random forest (RF) to screen LiDAR and Landsat 8 OLI multispectral indicators suitable for the prediction of plantation stock, and constructed a plantation stock model based on machine learning (support vector machine regression, RF regression). Our method is compared with traditional statistical methods (stepwise regression and partial least squares regression). Through the verification of 57 plantation field survey data, the accuracy of the stand estimation model constructed using the RF method is generally better (ΔR2 = 0.01~0.27, ΔRMSE = 1.88~13.77 m3·hm−2, ΔMAE = 1.17~13.57 m3·hm−2). The model evaluation accuracy based on machine learning is higher than that of the traditional statistical method, and the fitting R2 is greater than 0.91, while the fitting R2 of the traditional statistical method is 0.85. The best fitting models were all support vector regression models. The combination of UAV point clouds and satellite multi-spectral images has the best modeling effect, followed by LiDAR point clouds and Landsat 8. At present, this method is only applicable to artificial forests; further verification is needed for natural forests. In the future, the density and quality of higher clouds could be increased. The validity and accuracy of the method were further verified. This paper provides a method for predicting the accumulation of typical Chinese plantations at the forest farm scale based on the “airborne LiDAR + satellite remote sensing” data-driven machine learning modeling, which has potential application value for the current carbon neutrality goal of the southern plantation forest emission reduction project.

Nutritional contents and antimicrobial activity of the culinary-medicinal mushroom Leccinum scabrum

ABSTRACT Leccinum scabrum (Bull.) Gray (Boletaceae) is an edible mycorrhizal species with potential application interest due to its food and medicinal properties. A field investigation carried out during summer in the Białowieża Primeval Forest, located along the border between Belarus and Poland allowed to collect samples for chemical composition analysis and antibacterial activity evaluation. Mushroom extracts were prepared with microwave-assisted as well as ultrasound-assisted extraction techniques (UAE and MAE). The analysis of a dry sample of L. scabrum showed a significant content of vitamins and minerals and also a remarkable content of carbohydrates, protein, dietary fibre, total sugars, total free amino acids, and polyunsaturated fatty acids. The antibacterial activity of L. scabrum aqueous extracts showed inhibitory activity against all tested bacteria. In general, MAE extract exhibits a higher inhibition activity against Listeria monocytogenes ATCC 19114. As regards the Minimum Inhibitory Concentration (MIC) values, the high antibacterial activity of MAE extract was detected for L. monocytogenes ATCC 19114 and Escherichia coli ATCC 25922. Regarding UAE, high antibacterial activity was detected for Salmonella enterica ATCC 13076 and L. monocytogenes ATCC 19114. Based on data hereby reported, L. scabrum is a culinary-medicinal mushroom with a promising potential use as a high-quality food and nutraceutical mycological resource.

Different Approaches of Forest Type Classifications for Argentina Based on Functional Forests and Canopy Cover Composition by Tree Species

Modern forestry systems rely on typologies of forest types (FTs). In Argentina, several proposals have been developed, but they lack unified criteria. The objective was to compare different approaches, specifically focusing on (i) phenoclusters (functional forests based on vegetation phenology variations and climate variables) and (ii) forest canopy cover composition by tree species. We conducted comparative uni-variate analyses using data from national forest inventories, forest models (biodiversity, carbon, structure), and regional climate. We assessed the performance of phenoclusters in differentiating the variability of native forests (proxy: forest structure), biodiversity (proxy: indicator species), and environmental factors (proxies: soil carbon stock, elevation, climate). Additionally, we proposed a simple FT classification methodology based on species composition, considering the basal area of tree species. Finally, we compared the performance of both proposals. Our findings showed that classifications based on forest canopy cover composition are feasible to implement in regions dominated by mono-specific forests. However, phenoclusters allowed for the increased complexity of categories at the landscape level. Conversely, in regions where multi-specific stands prevailed, classifications based on forest canopy cover composition proved ineffective; however, phenoclusters facilitated a reduction in complexity at the landscape level. These results offer a pathway to harmonize national FT classifications by employing criteria and indicators to achieve sustainable forest management and conservation initiatives.

Electrical Conductivity, Basic Cations and Organic Matter content of Soils under different Land use Practices in AkwaIbom State

Variation in electrical conductivity, basic cations and organic matter content of soils can be influenced by land use practices, and these parameters may be affected by another. The study was conducted to ascertain the electrical conductivity (EC), basic cations and organic matter content of soils under varying land use types and to assess the relationship between electrical conductivity and the other parameters. The land use types were intensively cultivated farmland (ICF), natural forest (NF), oil palm plantation (OPP) and gmelina plantation (GP). Stratified random sampling technic was used in the collection of soil samples from the land use types. Three (3) replicates of bulk soil were collected for each land use giving a total of twelve (12) observational units. Soil samples were prepared and analyzed in a laboratory and data generated were subjected to analysis of variance, regression analysis and descriptive statistics. There was significant (P≤0.05) variation in the measured parameter among soils of the land use types. Soils under ICF, OPP, and GP had similar electrical conductivity of 0.08 (dS/m), while NF had 0.10 (dS/m). Organic matter (OM) content of 13.3 g/kg, 19.0 g/kg, 15.3 g/kg and 18.7 g/kg were observed under ICF, NF, OPP and GP, respectively. Exchangeable calcium at ICL was 2.33 cmol/kg; at NF it was 2.50 cmol/kg; at OPP, it was 2.40cmol/kg, and 1.93cmol/kg at GP. Exchangeable magnesium (Mg) was highest (1.07 cmol/kg) at ICL but lowest (0.93cmol/kg) at GP. Similarly, ICF had highest (1.73cmol/kg) and NF had lowest (0.04 cmol/kg) for exchangeable sodium. Calcium and magnesium had significant (P≤0.05) positive relationships with electrical conductivity, while organic matter and sodium had negative but non-significant relationship with electrical conductivity. The soils do not have salt problem and may not be prone to salinity in the future, meanwhile, organic matter input should be increased at the soil under intensively cultivated farmland.

Influence of Land Use Types on Soil Properties and Soil Quality in Karst Regions of Southwest China

Establishing a suitable and useful soil quality index (SQI) is the key to accurately evaluating changes in soil quality (SQ) under different land use types. In the present study, a suitable and useful SQI using a minimum data set (MDS) with two scoring methods (linear scoring method and nonlinear scoring method) and two additive models (simple additive model with same weighting value and weighted additive model with significant different weighting value) was established to compare SQ under different land uses in Longtan valley. Soil samples were collected under one dryland (DRYL), one paddy (PADD), one orchard (GRA), and one natural forest (FORE), and 13 soil properties were measured. The four land use types had the same soil type and similar environmental conditions. Land use types had significant effects on the measured 12 soil properties. The top two principal components in Principal Component Analysis were chosen, and their cumulative variance was more than 90%. Soil indicators of soil labile carbon, C/N ratio, and microaggregates were chosen as members of MDS in this study. Significant (p < 0.001) positive correlations among the four establishing SQIs were found. The values of the sensitive index ranged from 47.17% to 82.12% for the four SQIs, and the SQI established using the nonlinear scoring method and weighted additive model (SQI-NLW) had the highest values. Among the four land use types, the four SQIs had similar change trends, and the average values of SQ under FORE (0.73) and PADD (0.68) were significantly higher than those under GRA (0.54) and DRYL (0.43). These results indicated that the SQI-NLW was an effective and precise tool to assess SQ under different land uses in similar regions, and the FORE and PADD were the suitable land use types for the sustainable use of soils in karst regions.

Tree parameter extraction method based on new remote sensing technology and terrestrial laser scanning technology

Ground LiDAR is a terrestrial LiDAR system that is often used for terrain and geomorphic mapping. Ground-based LiDAR can be used to collect more local and short-range data, making it ideal for mapping smaller areas with high precision. In order to solve the rapid extraction of tree parameters in the national public welfare forest survey, the ground-based LIDAR was used to obtain the point cloud of trees, and the point cloud data was registered, denoised, normalized, sliced, parameter extracted, etc., and the parameters of individual trees in the forest were obtained. The Bland-Altman consistency test is used to test whether the method of extracting tree parameters from point clouds is consistent with the traditional measurement method. The experimental results show that the point cloud data obtained by the ground-based LIDAR can quickly, conveniently and accurately extract the tree parameters, which is consistent with the traditional tree parameter extraction method, and has the advantages than the traditional tree parameter measurement, such as point cloud, image and traceability. It has a unique advantage in establishing a tree database. It is suggested that LIDAR should be used for forest survey in the future.

Morphological description of the White-throated treerunner (Pygarrhichas albogularis, King 1831) in the Cape Horn Biosphere Reserve, Chile

The sub-Antarctic Magellanic ecoregion is one of the most pristine wilderness areas remaining on the planet, and is home to the southernmost forest ecosystems in the world, which are protected by the Cape Horn Biosphere Reserve (CHBR). In these forests, birds are the group of vertebrates with the largest number of species. However, essential aspects of the ecology and morphology of several species in this region are still understudied. These species include the White-throated treerunner (Pygarrhichas albogularis, King 1831), considered a “living fossil” as it is the only living species of the genus Pygarrhichas. In addition, this Furnariid is endemic to the temperate forests of South America. Using the 23-year database of the Long-Term Ornithological Research Program of Omora Park (54º56’S, 67º38’W), based on monthly captures and banding of forest birds, we describe the morphology, longevity, and presence of the White- throated treerunner in the CHBR. Between 2000 and 2022, 91 individuals were captured, including 29 recaptures. Based on these recaptures we determined that this species can live for at least five years. Morphometric measures showed a positive correlation between bill measures and tarsus length with both tail and wing lengths. The average weight varied for each season but not significantly. The greater presence on old-growth forests sites suggests a dependence of treerunners on large trees. This study expands the knowledge about the natural history of the White-throated treerunner, particularly about its populations inhabiting the world’s southernmost forests

Dating of avalanches in Pirin mountains in Bulgaria by tree-ring analysis of Pinus peuce and Pinus heldriechii trees

Snow avalanches are among the most important disturbances in mountain ecosystems. The effects of avalanches on the endemic Pinus heldreichii and Pinus peuce forests in the Pirin Mountains in Bulgaria are poorly understood. These forests are among the few remaining old-growth forests with preserved natural dynamics in Europe. However, the ongoing development of tourist infrastructure at the borders of some of the best-preserved Pinus heldreichii and Pinus peuce forests close to Bansko ski resort leads to increased tourist pressure. Many backcountry skiers and snowboarders venture beyond the resort boundaries, practicing their activities in these forests and avalanche tracks and couloirs within them. The objective of our study was to collect tree-ring cores from Pinus peuce and Pinus heldreichii trees affected by avalanches and verify the specific types of damages and tree responses characteristic for the species and region. Then using the collected data, we aimed at reconstructing the years in which specific areas were affected by avalanches and in this way obtain data on the magnitude of larger events. Our study shows that large magnitude avalanches (LMA) in the vicinity of Bansko ski resort in the Pirin Mountains in Bulgaria occurred dozens of times in the 20th century. On average the period between LMAs ranged between 9 and 20 years with the shortest period between two such events in one couloir being 2 years. Avalanches affected forests high on the couloir banks and left traces in the tree rings related to the mechanical impact that snow avalanches had on the surviving trees. In the studied tree-ring cores of Pinus peuce and Pinus heldreichii the most informative types of responses were sharp growth suppressions, including missing rings, followed by reaction wood and in less cases visible scars and callus tissue. Our data for frequent large avalanches shows that is necessary to address the avalanche risk with high priority especially for the Palashica couloir, which directly threatens the lower parts of the ski runs as well as other facilities in Bansko ski resort.

Wetland Creation and Reforestation of Legacy Surface Mines in the Central Appalachian Region (USA): A Potential Climate-Adaptation Approach for Pond-Breeding Amphibians?

Habitat restoration and creation within human-altered landscapes can buffer the impacts of climate change on wildlife. The Forestry Reclamation Approach (FRA) is a coal surface mine reclamation practice that enhances reforestation through soil decompaction and the planting of native trees. Recently, wetland creation has been coupled with FRA to increase habitat available for wildlife, including amphibians. Our objective was to evaluate the response of pond-breeding amphibians to the FRA by comparing species occupancy, richness, and abundance across two FRA age-classes (2–5-year and 8–11-year reclaimed forests), traditionally reclaimed sites that were left to naturally regenerate after mining, and in mature, unmined forests in the Monongahela National Forest (West Virginia, USA). We found that species richness and occupancy estimates did not differ across treatment types. Spotted Salamanders (Ambystoma maculatum) and Eastern Newts (Notophthalmus viridescens) had the greatest estimated abundances in wetlands in the older FRA treatment. Additionally, larger wetlands had greater abundances of Eastern Newts, Wood Frogs (Lithobates sylvaticus), and Green Frogs (L. clamitans) compared to smaller wetlands. Our results suggest that wetland creation and reforestation increases the number of breeding sites and promotes microhabitat and microclimate conditions that likely maximize the resilience of pond-breeding amphibians to anticipated climate changes in the study area.

TYPOLOGIES OF LAND USE AND LAND COVER ASSOCIATED WITH CASES OF CUTANEOUS LEISHMANIASIS IN A METROPOLITAN REGION IN THE BRAZILIAN AMAZON

Characterizing the probable site of infection of cutaneous leishmaniasis (CL) in face of anthropic changes in the Amazon makes it possible to understand the distribution of this disease in order to adopt control measures. The objective of this study is to identify landscape typologies resulting from changes in land use and land cover in the Metropolitan Region of Santarém regarding the occurrence of CL cases in 2012 and 2014. Landscape typologies were developed from TerraClass project data using regular 1 km² cells. Landscape presence and dominance metrics were used to generate cells with a single class and cells with more than one class, called mosaics. For cases of leishmaniasis, the metric was the presence of the disease. Association analyses were extracted from a 2x2 contingency table. The primary forest typology (PP04) had the highest number of cells in both years analyzed. However, changes in land use and land cover were evidenced by the growth in the number of cells with mosaics of agriculture (PP11 and PP12), urbanized areas (PP03 and PP10), and pastures (PP13). The presence of at least one case of CL in each year occurred in ten typologies, particularly in compositions with urbanized areas, pastures, and secondary vegetation. Typologies with the agriculture class, although the number of cells increased, did not follow the same growth logic regarding the presence of the disease. This study makes it possible to identify and characterize the places where CL occurs and provides further information for health surveillance agencies

Topographical gradient of the structure and diversity of a woody plant community in a seasonally dry tropical forest in northwestern Madagascar

AbstractFew studies have evaluated the structure and dynamics of forests in Madagascar with high levels of endemism. Ankarafantsika National Park holds the largest of the remaining primary dry forests in northwestern Madagascar, where most of the forests have been lost or degraded by fire and other human activities. In this primary forest, we established a 15‐ha forest dynamics monitoring plot and mapped and identified all woody stems with a diameter at breast height (DBH) ≥5 cm. The forest stand was characterized by small‐sized individuals (75% of stems with DBH <10 cm, with 99% of trees with height <15 m), encompassing 35,758 tree and 513 liana individuals belonging to 160 species in 53 families (including 20 species of lianas accounting for 1.4% of the stems). Most species‐rich families were Rubiaceae, Fabaceae, Lamiaceae, and Malvaceae, which together encompassed 43 tree species and 23% of all tree individuals. The plot was located on a hill consisting of white sand at elevations from 150 to 200 m, with a gentle slope down from southwest to northeast. Over this elevation gradient, stem density and species diversity increased with elevation, while tree height and diameter decreased. The most abundant two species, Drypetes perrieri (Putranjivaceae) and Noronhia alleizettei (Oleaceae) are evergreen, suggesting the importance of a nutrient conservation strategy. These results provide the foundational knowledge necessary for the conservation and restoration of natural semi‐deciduous dry forests that used to cover large areas in northwestern Madagascar until recently.

Stable isotopic composition of Bothrops atrox (common lancehead) in four distinct land covers in eastern Amazonia

AbstractSince consumers reflect the isotopic composition of an assimilated diet, stable isotopes can be a useful tool to address the feeding ecology of tropical snakes. This is the first study reporting carbon and nitrogen stable isotopic composition of Bothrops atrox (Linnaeus, 1758) living in different landscapes located in the lower Amazon river, encompassing four main natural landscapes of the Amazon: old‐growth forests, várzeas (flooded forests), savannas, and pastures. Our null hypothesis is that the δ13C of forest specimens of B.atrox is more negative because forests are dominated by C3 plants, while C4 plants are common in the other landscapes. On the other hand, δ15N of forest specimens should be more positive, since the δ15N of old‐growth forests are higher than plants of savanna, várzea, and pastures. Confirming our hypothesis, the δ13C of B. atrox scales of the Tapajós National Forest was approximate −25‰ to −24‰, increased to approximately −23.5‰ to −23.0‰ in the savanna and pasture, and to −21‰ in the várzea, showing an increased contribution of C4‐derived carbon. Some specimens of B. atrox had δ15N as high as 18‰, which is much higher than the average δ15N of the snake's prey (7‰), confirming the apex position of B. atrox in the Amazon region. The δ15N values of the forest specimens were 5‰ higher than the savanna specimens, and this difference decreased to 3‰ between the forest and the pasture, and the várzea specimens. Finally, there were not large differences between δ15N values of livers and scales in any of the landscapes, suggesting a constant diet through time, and reinforcing the possibility of the use of snake's scale as a less invasive and non‐lethal tissue to analyze.Abstract in Portuguese is available with online material.

Leaf functional traits and ecological niche of Fagus grandifolia and Oreomunnea mexicana in natural forests and plantings as a proxy of climate change.

Functional traits reflect species' responses to environmental variation and the breadth of their ecological niches. Fagus grandifolia and Oreomunnea mexicana have restricted distribution in upper montane cloud forests (1700-2000 m a.s.l.) in Mexico. These species were introduced into plantings at lower elevations (1200-1600 m a.s.l.) that have climates predicted for montane forests in 2050 and 2070. The aim was to relate morphological leaf traits to the ecological niche structure of each species. Leaf functional traits (leaf area, specific leaf area [SLA], thickness, and toughness) were analyzed in forests and plantings. Atmospheric circulation models and representative concentration pathways (RCPs: 2.6, 4.5, 8.5) were used to assess future climate conditions. Trait-niche relationships were analyzed by measuring the Mahalanobis distance (MD) from the forests and the plantings to the ecological niche centroid (ENC). For both species, leaf area and SLA were higher and toughness lower in plantings at lower elevation relative to those in higher-elevation forests, and thickness was similar. Leaf traits varied with distance from sites to the ENC. Forests and plantings have different environmental locations regarding the ENC, but forests are closer (MD 0.34-0.58) than plantings (MD 0.50-0.70) for both species. Elevation as a proxy for expected future climate conditions influenced the functional traits of both species, and trait patterns related to the structure of their ecological niches were consistent. The use of distances to the ENC is a promising approach to explore variability in species' functional traits and phenotypic responses in optimal versus marginal environmental conditions.

Conventional and Organic Honey Production and Quality: A Worldwide Perspective

Theoretical Framework: Honey production in the country is mainly carried out by family farmers, contributing to sustainable development and biodiversity. Organic honey production is a relevant topic, driven by the movement towards healthy foods. Brazil leads global organic honey production, standing out for being produced in native forest areas without the use of pesticides. Organic certification plays a crucial role, ensuring product quality, free from chemical contaminants, and promoting sustainable practices. Objective: To deepen the understanding of honey production and quality, focusing on the characteristics of traditional and organic honey, production at both global and Brazilian levels, beekeeping practices, family farming, and the production and certification of organic honey. Methodology: The Methodi Ordinatio protocol was used, a theoretical approach methodology based on literature review from platforms such as CAPES, WebSciense, Google Scholar, and PubMed, for the selection of scientific articles. Results and Discussion: The analysis highlighted the economic importance of honey and the challenges in organic production, such as the need for technical assistance and access to technologies. In summary, the article provides a comprehensive view of honey production and quality, emphasizing the relevance of organic production and certification as essential factors for sustainable practices.

First report of the barley root-knot nematode, Meloidogyne naasi Franklin, 1965 from Pennsylvania, with new host report.

Meloidogyne naasi Franklin, 1965, the barley root-knot nematode, was originally found in field crops such as cereals, grasses, and sugar beet (Beta vulgaris L.) in England and Wales, (Franklin,1965). This nematode is one of the most significant root-knot nematodes impacting grains in European countries (Santos et al. 2020). Among root-knot nematode species, M. naasi, exhibits a distinct preference for grasses, with documented impacts on turfgrasses leading to reduced growth and vigor (Skantar et al., 2023; Cook and Yeates, 1993). In September 2022, root-knot nematode females and second-stage juveniles (J2) were recovered from roots of fowl manna grass, Glyceria striata (Lam.) Hitchc., during a nematode survey on natural vegetation at the Allegheny National Forest (41°30'13.8"N 79°09'46.2"W). Second-stage juvenile specimens were recovered from soil using sugar centrifugal flotation (Jenkins, 1964). Small galls with egg masses were dissected from fowl manna grass roots originally collected from the surveyed areas. In parallel, five plants of non-infected fowl manna grass were placed in a pot in the greenhouse using naturally nematode-infested soil collected from the same forested area. Small galls and female specimens recovered from these plants were dissected and processed for further analyses. Female and J2 were fixed in 3% formaldehyde solution and processed to glycerin (Golden, 1990; Hooper, 1970). The specimens were examined by light microscopy, morphometric measurements, and molecular markers, which included the D2-D3 region of the large ribosomal subunit 28S, and the rDNA internal transcribed spacer region (ITS). The perennial pattern of five females analyzed morphologically were consistent to the patterns observed for M. naasi. The perennial patterns had coarse ridges on the cuticle in dorsal region forming broken irregular lines around anal and phasmid area. We also noted a prominent fold that covered some of the anus and showed a curved line between vulval slit and phasmids, typical of M. naasi. The area around the vulval area had a few or no striae except for a few lines radiating from the vulval slit as in the original description. Measurements of ten J2 had a body length ranged between 380 and 410 µm, stylet 11-13 µm, tail 50-70 µm long with a hyaline tail terminus between 12-22 µm in length, 4 lines in the lateral field, a and c ratio between 29.23-35.91 and 5.79-7.9 fitting the original description by Franklin, 1965 and others populations found in the USA (Skantar et al., 2023). The matrix codes for the female specimes are A32, B324, C3, D3 and for J2's A2, B21, C123, D1, E3, F12 (Subbotin et al., 2021). The amplified DNA fragments were sequenced, resulting in an 726 bp fragment flanked by the D2-D3 primers (PP097762), while for the ITS primers an 634 bp fragment was obtained (PP092043). Both generated sequences for the specimens collected in Pennsylvania revealed >99% similarity to M. naasi sequences deposited at GenBank, and therefore, validating the morphological analyses. Based on both morphological and molecular analyses the specimens collected in the state of Pennsylvania were identified as M.naasi. To our knowledge, this is the first report of this species from this state and being associated with naturally infected fowl manna grass.

Mammal species biodiversity in a Costa Rican protected tropical rainforest environment

Tropical rainforest deforestation has led to old-growth forest loss and declines in species diversity worldwide. In the 1980’s, Costa Rica began innovative reforestation efforts and currently 52% of Costa Rica is a tropical forest environment. However, the impact of Costa Rica’s reforestation efforts on regaining species biodiversity is less known. The aim of this study was to consider longitudinal changes in mammal biodiversity at La Selva Biological Station (LSBS) following reforestation. Mammal species census data were collected between 1994 and 2017 by 30-60 trained and experienced Citizen Scientists. We calculated the total number of different species sighted per year, their relative abundance, and the Shannon Index to estimate biodiversity. Linear regression was used to consider change over time and ANOVA was used to consider differences in land and water use by mammal species (p<0.05). Results suggest the total number of different species observed declined, their relative abundance declined, and the Shannon index of biodiversity declined (p<0.05). This decline is similar to that reported for avian fauna, terrestrial amphibian, and dung beetle species reported at LSBS. Our study highlights the concern that past deforestation continues to lead to species decline and the ongoing need for tropical forest stewardship.

Female Deer Movements Relative to Firearms Hunting in Northern Georgia, USA.

Perceived risk associated with hunters can cause white-tailed deer (Odocoileus virginianus) to shift their activity away from key foraging areas or alter normal movements, which are important considerations in managing hunting and its effects on a population. We studied the effects of seven firearms hunts on the movements of 20 female deer in two Wildlife Management Areas within the Chattahoochee National Forest of northern Georgia, USA, during the 2018-2019 and 2019-2020 hunting seasons. Deer populations and the number of hunters in our study area have declined significantly since the 1980s. In response, hunting regulations for the 2019-2020 hunting season eliminated opportunities for harvesting female deer. To evaluate the indirect effects of antlered deer hunting on non-target female deer, we calculated 90% utilization distributions (UDs), 50% UDs, and step lengths for pre-hunt, hunt, and post-hunt periods using the dynamic Brownian bridge movement model. Data included 30 min GPS locations for 44 deer-hunt combinations. Pre-hunt 50% UDs (x- = 7.0 ha, SE = 0.4 ha) were slightly greater than both hunt (x- = 6.0 ha, SE = 0.3 ha) and post-hunt (x- = 6.0 ha, SE = 0.2 ha) 50% UDs (F = 3.84, p = 0.03). We did not detect differences in step length, nor did we detect differences in size or composition of 90% UDs, among the periods. Overall, our results suggest that the low level of hunting pressure in our study area and lack of exposure to hunters led to no biologically significant changes in female deer movements. To the extent of the findings presented in this paper, adjustments to the management of hunting in our study area do not appear to be necessary to minimize hunting-related disturbances for female deer. However, managers should continue to consider female deer behavior when evaluating future changes to hunting regulations.