Native Chicken Research Articles

Evaluating sperm quality characteristics obtained through the female teaser method in native chicken breeds

Evaluating sperm quality characteristics obtained through the female teaser method in native chicken breeds

Performance Of Balitbangtan Native Chicken With Various Level of Leucaena leucocephala Leaf Flour in Feeds

Feed is the largest production input at the farm, include in native chiken farm. Ingredieent of feed devine in two part, there are as resource energy and resoursce protein. Protein source feed are relatively more expensive than energy source. Lamtoro leaves (Leucaena leucocephala) is a legume source of protein that can grow well in tropical and dry climates. The crude protein content of lamtoro leaf flour can reach above 20% so that it has the potential as a protein source feed ingredient for chicken livestock. This research was conducted to determine the level of proportion of lamtoro leaf meal in chicken feed, in this case the Balitbangtan superior native chicken (KUB) without affecting its performance. The combination of 4 treatments with different proportions of lamtoro leaf meal in the feed, namely P0 = 0%, P1 = 5%, P2 = 10% and P4 = 15%, with each treatment consisting of 3 groups containing 5 KUB chickens aged 4 weeks with initial body weight 247 – 255 g. Length of maintenance/research 8 weeks (up to 12 weeks of age). Parameters observed were feed consumption, final body weight, daily body weight growth, and feed conversion. The results showed that there was no significant difference between treatments (P>0.05) both in the level of feed consumption, daily body weight gain, and feed conversion. It can be concluded that lamtoro leaf flour can be used up to a level of 15% in the grower phase of KUB chicken livestock rations.

Thermal impacts on transcriptome of Pectoralis major muscle collected from commercial broilers, Thai native chickens and its crossbreeds.

The main objective of this study was to define molecular mechanisms associated with thermal stress responses of chickens from commercial broilers (BR, Ross 308), Thai native chickens (NT) and crossbreeds between BR×NT (H75). Twenty days before reaching specific market age, chickens from each breed were divided into control and thermal-stressed groups. The stressed groups were exposed to a cyclic thermal challenge (35°C±1°C for 6 h, followed by 26°C±1°C for 18 h) for 20 days. Control group was raised under a constant temperature of 26°C±1°C. Pectoralis major (n = 4) from each group was collected for transcriptome analysis using HiSeq Illumina and analysis of glycogen and lactate. Gene expression patterns between control and thermalstressed groups were compared within the same breeds. Differentially expressed transcripts of 65, 59, and 246 transcripts for BR, NT, and H75, respectively, were revealed by RNA-Seq and recognized by Kyoto encyclopedia of genes and genomes database. Pathway analysis underlined altered glucose homeostasis and protein metabolisms in all breeds. The signals centered around phosphatidylinositol 3-kinase (PI3K)/Akt signaling, focal adhesion, and MAPK signaling in all breeds with slight differences in molecular signal transduction patterns among the breeds. An extensive apoptosis was underlined for BR. Roles of AMPK, MAPK signaling and regulation of actin cytoskeleton in adaptive response were suggested for H75 and NT chickens. Lower glycogen content was observed in the breast muscles of BR and NT (p<0.01) compared to their control counterparts. Only BR muscle exhibited increased lactate (p<0.01) upon exposure to the stress. The results provided a better comprehension regarding the associated biological pathways in response to the cyclic thermal stress in each breed and in chickens with different growth rates.

IPTEKS BAGI MASYARAKAT KELOMPOK PETERNAK AYAM KAMPUNG PADA PETERNAKAN DI NEGERI RUTONG KOTA AMBON

Native chicken farms can support the community's economy, there are many in rural areas and are generally traditional. Human intervention in maintenance is limited to ownership, housing and limited feed. The negative impact of maintenance like this is low production. Objectives: 1. Increase chicken production, utilize fisheries and agricultural waste (sago dregs, cassava leaves, lamtoro leaves). 2. Increase the knowledge and skills of breeders on how to process feed ingredients and prepare rations. 3. Train and motivate farmers to improve maintenance systems and develop their livestock business. The target of this activity is a group of native chicken breeders (Dasawisma group). The success of this activity is measured through better farmer attention (80%) to the livestock being kept and better production. Treatment results on IbM livestock rations resulted in egg production and egg quality which were 25-40% better, and DOC weight was quite high compared to the extensive method (conventional feed). Groups can have increased knowledge about feed and applicable hatchery technology

Designing and evaluating a cost‐effective single nucleotide polymorphism liquid array for Chinese native chickens

AbstractGenotyping plays an important role in breeding and population studies. Currently available genotyping technologies, including solid‐phase chips and sequencing, often have several limitations in their application to local chickens in China, including high costs, insufficient diversity, and poor universality. In the present study, we developed a chicken 5 K single nucleotide polymorphism (SNP) array suitable for breeding and genetic analysis using genotyping by targeted sequencing technology. The chip design was based on genomic data from 33 local breeds, and 5847 SNPs were selected for the final chip design. Among these SNPs, 3427 sites were associated with economic traits in broiler chickens. Our chip contained 25,000 high‐quality SNP markers captured from 5 K regions with highly efficient target site capture. Population analyses of the eight breeds showed high detection rates and minor allele frequencies for SNP markers on the array, enabling clear differentiation of different populations and families within populations. The genetic diversity trend obtained using the chip was consistent with the results obtained from microsatellite analyses. Additionally, the 5 K array was applied to a genome‐wide association study of broilers, resulting in the identification of several growth‐ and meat quality‐related loci. Therefore, the newly developed chip is efficient, cost‐effective, and well suited for application in local Chinese chickens, which will accelerate poultry breeding improvements and enhance conservation efforts.

Nutrien dan Morfologi Membran Kerabang Telur Ayam Kampung sebagai Bahan Baku Produksi Kolagen

Collagen derived from the eggshell membrane, is an increasingly popular functional material. Native chicken is one of the leading local commodities whose industrial waste has the potential to be used as collagen raw material. This study aimed to evaluate the nutrients and morphology of the eggshell membrane of native chicken eggs as raw material for collagen production. The parameters observed were morphology using electron scanning microscopy, mineral composition, and collagen nutrient content (water and protein). SEM observations showed that the eggshell membrane of native chicken eggs was fibrous. The meshed fiber form represents the extracellular matrix present in collagen proteins. The eggshell membrane contains Cl, K, Ca, P, and Mg macrominerals at 1.696%, 1.796%, 14.30%, 0.437%, and 0.146%, respectively. Microminerals I and Mo were also found at 0.00034% and 0.00014%, respectively. The moisture content of the superior native chicken eggshell membrane decreased by 13.05% after the collagen extraction (p&lt;0.05), with a crude protein content of 25.32% after the extraction. It was concluded that the eggshell membrane of native chicken eggs produces fibrous morphological characteristics that are meshed and contain high minerals so that they have the potential to be used as raw materials for collagen production in various applications.&#x0D; &#x0D; Keywords: collagen, eggshell, eggshell membrane, native chicken, morphology

The characterization and correlation between the phenotypic and genotypic resistance of Campylobacter spp. isolates from commercial broilers and native chickens in the south of Thailand

ABSTRACT Chicken is a major reservoir and source of human infections from zoonotic Campylobacter. In southern Thailand, chicken is the most popular meat consumed; however, little is known about the antimicrobial resistance (AMR) of Campylobacter spp. in chicken production systems. We aimed to investigate the prevalence and characteristics of AMR in Campylobacter isolates from commercial broiler and native chicken farms in southern Thailand. A total of 286 Campylobacter isolates (adjusted prevalence: 60.3%, SE ± 0.01) were recovered, of which 268 were identified as Campylobacter jejuni and 18 as Campylobacter coli. The adjusted prevalence of resistance was highest for nalidixic acid (98.6%), followed by ciprofloxacin (97.2%), enrofloxacin (96.4%), tetracycline (74.6%), tylosin (38.3%), and erythromycin (10.6%). Commercial broilers had resistance levels 7–11% higher to quinolones and 35–47% lower to macrolides and tetracycline (all P ≤ 0.025) compared to native chickens. Genotypic resistance analyses revealed high prevalence rates (>86%) for gyrA mutant, efflux pump CmeABC, and point mutation A2075G in the 23S rRNA gene. High homogeneity was observed among isolates within study farms. Commercial broilers exhibited higher homogeneity in AMR patterns than native chickens. The best agreement was observed between tetracycline resistance and tet(O) genes (Kappa: k = 0.811, P < 0.001), while the fair agreement was found for quinolone resistance and gyrA mutant genes (k = 0.260–0.271, P < 0.001). No association was detected between macrolide resistance and point mutations in the 23S rRNA gene. These findings fill knowledge gaps on AMR in Campylobacter in Thailand, emphasizing the need for targeted AMR mitigation strategies in both commercial broilers and native chickens. RESEARCH HIGHLIGHTS High Campylobacter prevalence in chickens; C. jejuni more prevalent than C. coli. Susceptibility to macrolides but resistance to quinolones/tetracyclines in isolates. Homogeneous resistance patterns within farms; higher in broilers than in native birds. Partial association between phenotypic and genotypic resistance among isolates.

Digestibility of Native Chicken Rations Containing Fermented Banana Peels

Improving the performance of super native chickens must be balanced by improving the ration. The ingredients used do not need to be “expensive” but are of good quality and readily available throughout the year. One of the feed ingredients that is widely available and is a waste is banana peel. Banana peels contain 3.63% nutrients, 2.52% crude fat, 18.71% crude fiber, 7.18% calcium, and 2.06% phosphorus. In addition to high crude fiber, banana peels also contain antinutrients in the form of tannins, hence the need for fermentation. To see the quality of the ration, it is necessary to test the digestibility. This research was carried out from March to July 2023. The design used in this study was a completely randomized design (CRD) with 5 (five) treatments and 3 (three) replicates. The treatments were as follows: R0 (ration without the addition of fermented banana peel flour/TKPF), R1 = (2.5% TKPF), R2 = 5% TKPF, R3 = 7.5% TKPF, R4 = 10% TKPF. Variables observed were N retention, protein digestibility, crude fiber digestibility, and organic matter digestibility. The results showed that the provision of fermented banana flour in the ration of native chickens significantly (P &lt;0.01) influenced the digestibility of crude protein, crude fiber digestibility, digestibility of organic matter, and N retention of native chicken rations. The best results were obtained in the R2 treatment (5% Fermented Banana peel flour), where the crude protein digestibility gave results of 76.11%, crude fiber digestibility of 45.12%, organic matter digestibility of 70.09%, and N retention of 75.82%

The Effect Of Giving Amino Acid Solutions Based On Maggot Bsf (Hermetia Illucians) With Different Concentrations Into The Feed On The Percentage Of Breast And Back Of Kub Chickens

JIRFAN DAWANTO (4517035005). The Effect of Giving Amino Acid Solutions Based on BSF Maggots (Hermetia Illucians) with Different Concentrations into the Feed on the Percentage of Breast and Back of KUB Chickens. (Under the guidance of Asmawati Mudarsep as the main guide and Muhammad Idrus as the aggota guide).&#x0D; KUB chickens are widely kept by farmers because they have fast growth. The current constraint on native chicken farming is the high price of feed raw materials. Therefore, Maggot from the Black Soldier Fly fly (Hermetia Illucens) is one of the best alternatives as a mixture of chicken feed because it has a nutrient composition that is almost the same as fish meal. Based on this, this study aims to determine the Effect of Giving Amino Acid Solutions Based on BSF Maggot (Hermetia Illucians) with Different Concentrations into Feed on the Percentage of Breast and Back of KUB Chickens.&#x0D; This study used the DOC of 96 KUB chickens and was kept for 60 days with a cage map of 16 plots and each plot consisted of 6 KUB chickens. Feed used at the age of 1-30 days is given BP-11 granules, then at the age of 31-60 days using mixed feed by adding a fermented Maggot-based amino acid solution arranged according to treatment.&#x0D; The treatment used is the administration of BSF maggot-based amino acid solutions , namely: P0, P1 (15 ml), P2 (30 ml), P3 (45 ml)&#x0D; The results of the analysis of various doses of Maggot amino acid solution as an additive to mixed feed showed results that had no real effect (P&gt;0.05) on the percentage of breast and back of KUB chickens. However, the administration of Maggot amino acid solution as an additive to mixed feed did not reduce the weight of KUB chicken breasts and buttocks and showed good performance

Integrated Analysis of the Effects of Cecal Microbiota and Serum Metabolome on Market Weights of Chinese Native Chickens.

The gut microbiota plays an important role in the physiological activities of the host and affects the formation of important economic traits in livestock farming. The effects of cecal microbiota on chicken weights were investigated using the Guizhou yellow chicken as a model. Experimental cohorts from chickens with high- (HC, n = 16) and low-market-weights (LC, n = 16) were collected. Microbial 16S rRNA gene sequencing and non-targeted serum metabolome data were integrated to explore the effect and metabolic mechanism of cecal microbiota on market weight. The genera Lachnoclostridium, Alistipes, Negativibacillus, Sellimonas, and Ruminococcus torques were enriched in the HC group, while Phascolarctobacterium was enriched in the LC group (p < 0.05). Metabolomic analysis determined that pantothenic acid (vitamin B5), luvangetin (2H-1-benzopyran-6-acrylic acid), and menadione (vitamin K3) were significantly higher in HC serum, while beclomethasone dipropionate (a glucocorticoid) and chlorophene (2-benzyl-4-chlorophenol) were present at higher levels in the LC group. The microbes enriched in HC were significantly positively correlated with metabolites, including pantothenic acid and menadione, and negatively correlated with beclomethasone dipropionate and chlorophene. These results indicated that specific cecal bacteria in Guizhou yellow chickens alter the host metabolism and growth performance. This study provides a reference for revealing the mechanism of cecal microbe actions that affect chicken body weight.

Comparative study and relationship analysis between purine content, uric acid, superoxide dismutase, and growth traits in purebred and crossbred Thai native chickens.

The objective was to compare and analyze the relationship between growth, purine content, uric acid, and superoxide dismutase (SOD) in purebred and crossbred Thai native chickens. A total of 300 Thai native chickens were divided into 3 groups. Group 1 was purebred Thai native chickens (100%TN), Group 2 was 50% Thai native chickens (50%TN), and Group 3 was 25% Thai native chickens (25%TN). Data included the body weight (BW), average daily gain (ADG), and breast circumference (BrC). At 6, 8, and 10 weeks of age, 10 chickens from each group were randomly euthanized to collect breast meat, liver, and blood samples to analyze the purine content consisting of total purine, adenine, guanine, xanthine, and hypoxanthine, and uric acid, in breast meat and liver and SOD in blood. A general linear model, Pearson correlation and principal component analysis were used to analyze the significant differences and relationship between variables. The results showed the 25%TN group had the highest growth traits at every age, while the 100%TN group had the lowest (p < 0.05). Consistent with the analysis results of purine values, purine content and uric acid in breast meat and liver and SOD in blood decreased with age (p < 0.05). The correlations between purine content (total purine, adenine, guanine, xanthine, and hypoxanthine) and growth traits (BW, ADG, and BrC) ranged from moderate negative to moderate positive (-0.542 to 0.253)(p < 0.05). The correlations between uric acid and growth traits (0.348-0.760) and SOD and growth traits (0.132-0.516) were low to moderate positive with significant differences (p < 0.05). The principal component plot, which highlighted three principal components (PC 1, PC 2, and PC 3), explained 86.44 and 86.53% of the total information in breast meat and liver for selecting animals for optimal balance of the variation in the growth traits, purine content, uric acid, and SOD. Although purebred Thai native chickens showed the lowest growth traits, purine content, uric acid, and SOD were also lowest compared to crossbred Thai native chickens. Therefore, the development of genetics in Thai native chickens to produce healthy food could be possible.

A Review on Production, Reproduction, Morphometric, and Morphological Characteristics of Ethiopian Native Chickens

Native chickens in Ethiopia are characterized in a fragmented manner for their performance characteristics and genotypes. This review aimed to explore the production and reproduction performance characteristics as well as the morphometric and morphological diversity of Ethiopian native chickens. The investigation was performed on four production performance characteristics, including average egg per clutch, average clutch/hen/year, average egg set/hen, and average egg/hen/year, as well as six reproductive performance characteristics, including age at first laying, age of male chickens at first bred, age at which female chickens are first bred, the reproductive life span of males and females, and fertility percentage in various parts of Ethiopia. Some economically practical morphometric characteristics of native chickens, such as shank length, chest circumference, comb length, body weight, body length, keel length, wattle length, neck length, back length, and morphological diversity, were also summarized. Regarding performance characteristics, there were some variations in eggs’ average production performance per clutch (13.56-15.4 eggs) and clutch/hen/year (3.0-4.29) in Ethiopia. The average reproduction performance characteristics of Ethiopian native chickens for age at first laying (6.90-7.13 months), age of male chickens at first bred (5.87-6.15 months), female at first bred (5.20-5.93 months), the reproductive life span of males (3.79 years) and hens (3.56 years), and chicks hatched from set eggs revealed variation across Ethiopia. In various locations of Ethiopia, the average trait values reported for Ethiopian native chickens under the farmer’s management differed in terms of morphometric and morphological features. The variation observed in performance characteristics, as well as morphometrics and morphological characteristics for Ethiopian native chicken ecotype population, can help the native breed classification, unique trait conservation, and breed improvement intervention programs.

Evaluation of Semen Quality of Free-Range Chickens After Addition of Putri Malu Leaf Herbal Extract (Mimosa pudica Linn)

This research was conducted to determine the effect of female shy daughter (Mimosa pudica Linn) leaf extract supplementation on the semen quality of native chickens. In this study, 18 male native chickens aged approximately 1 year with an average weight of 2.0 kg were used, divided into 6 treatments, each with 3 replications, each consisting of 1 chicken. Basal feed in this study used BR-1 chicken feed produced by PT. Japfa Comfeed Indonesia Tbk. As an adjustment before treatment, the chickens were reared for 1 week with basic feed. As a control before being given treatment, sperm was taken from chickens and used as a control. Furthermore, the chickens began to be given treatment by giving shame daughter leaf extract supplementation. The study was conducted experimentally using a completely randomized design by allocating 18 free-range male chickens which were divided into 6 treatments, each treatment consisting of three replications, one chicken each. The conclusion of this study is that the supplementation of Putri Mal leaf extract as much as 0.50 cc to 2 cc per day per chicken can increase sperm count per ml of free-range chicken semen, viability, motility, thickness, and color density, while the volume and pH of semen do not experience change.

Posthatch developmental changes in the ovary with emphasis on follicular development and atresia in the native chicken (Uttara fowl) of Uttarakhand, India.

This experiment was designed to investigate the postnatal development of the ovary in the Uttara fowl chicken and was conducted on 54 apparently healthy female birds divided into different age groups, namely Day 1 and Weeks 1, 4, 8, 12, 16, 20, 24, 28 with six birds each. During postnatal development, the left ovary gradually increased in size and complexity. The segmentation of the ovary started by 4 weeks, follicular eruption by 8 weeks, small liquor follicles (1-5 mm) appeared by 16 weeks, pre-hierarchical follicles by 20 weeks and hierarchical follicles by 24 weeks of age. The cortex was distinctly differentiated from the medulla in the early stage of ovarian development. However, the division between cortex and medulla was gradually obscured with age (transitional stage) and distinction was completely lost in the mature ovary. The different stages of follicular development in the chicken ovary were classified as primordial, primary, growing at Stage I, II and III stromal follicles besides pre-hierarchical and hierarchical surface follicles. The primordial and primary follicles showed cytoplasmic sudanophilic substances, especially in the Balbiani's yolk body, indicating the presence of lipids (Sudan Black B) with no activity for neutral polysaccharides (periodic acid Schiff method). It was observed that apoptotic changes may affect any stage of developing follicle resulting in arrested growth and atrophy. An early form of follicular atresia was the fate of the growth-arrested primordial and primary follicles, whereas the glandular form of atresia was commonly observed in growing follicles arrested at Stages I and II. The scanning electron micrographs unveiled the follicles as hollow oval structures with a follicular lumen lined by the perivitelline membrane (glycoprotein membrane) having lacunae giving a honeycomb-like appearance.

Genome-Wide Association Study on the Content of Nucleotide-Related Compounds in Korean Native Chicken Breast Meat.

Meat flavor is an important factor that influences the palatability of chicken meat. Inosine 5'-monophosphate (IMP), inosine, and hypoxanthine are nucleic acids that serve as taste-active compounds, mainly enhancing flavor in muscle tissue. For this study, we performed a genome-wide association study (GWAS) using a mixed linear model to identify single-nucleotide polymorphisms (SNPs) that are significantly associated with changes in the contents of the nucleotide-related compounds of breast meat in the Korean native chicken (KNC) population. The genomic region on chicken chromosome 5 containing an SNP (rs316338889) was significantly (p < 0.05) associated with all three traits. The trait-related candidate genes located in this significant genomic region were investigated through performing a functional enrichment analysis and protein-protein interaction (PPI) database search. We found six candidate genes related to the function that possibly affected the content of nucleotide-related compounds in the muscle, namely, the TNNT3 and TNNT2 genes that regulate muscle contractions; the INS, IGF2, and DUSP8 genes associated with insulin sensitivity; and the C5NT1AL gene that is presumably related to the nucleotide metabolism process. This study is the first of its kind to find candidate genes associated with the content of all three types of nucleotide-related compounds in chicken meat using GWAS. The candidate genes identified in this study can be used for genomic selection to breed better-quality chickens in the future.

Bias and accuracy of body weight trait evaluations of an F2 chicken using single-step genomic best linear unbiased prediction (ssGBLUP)

The objectives of this study were (i) to compare the accuracy and bias of estimates of breeding values for body weight (BW) at 2–7 weeks of age using pedigree-based best linear unbiased prediction (BLUP) and single-step genomic BLUP (ssGBLUP) methods, and (ii) to determine the best level of minor allele frequencies (MAFs) for pre-selection of SNPs for genomic prediction (GP). Records of 488 F2 broiler chickens obtained from crossbreeding of fast-growing Arian chickens and slow-growing Iranian native chickens at 2–7 weeks of age were used. Samples were genotyped using Illumina Chicken 60K BeadChip. To investigate the effect of MAFs on the accuracy of prediction, 48 379 quality-controlled SNPs were grouped into five subgroups with MAF bins 0.05–0.1, 0.1–0.2, 0.2–0.3, 0.3–0.4, and 0.4–0.5. Our results confirmed the superiority of ssGBLUP compared to traditional BLUP methodology. The average accuracy of GP improved by 59.03%, 220.34%, 0.46%, 5.61%, 0.45%, and 2.73% using ssGBLUP compared to BLUP for BW at 2–7 weeks of age, respectively. Depending on the age group, using a subset of SNPs with a specific MAF bin compared to all SNPs resulted in a remarkable improvement of GP accuracy for the observed traits.

Integrasi Pasar Jangka Pendek Ayam Broiler di Indonesia

One of the fast-growing livestock subsectors is the poultry sector. This livestock subsector produces broiler chicken meat as a strategic commodity in addition to beef, milk, and eggs. The level of broiler chicken meat consumption is the highest order, followed by native chicken consumption, then beef. Broiler meat is a rapidly growing strategic commodity. This study uses ECM model analysis to determine the short-term market integration in broiler chickens—time series data on broiler chicken prices at the producer level between January 2015 and December 2020. This analysis's first step is testing broiler chicken meat producer price data stationarity in 28 provinces. Data that are not stationary will be heteroscedastic (have autocorrelation). In addition, time series data that are not stationary can also produce spurious regression, a condition in which two or more variables have a causality relationship, but there is no theory or logic underlying the direct causality relationship between two or more of these variables. According to the findings of this investigation, there is no complete integration. According to the study's findings, the weak flow of information in the market is suspected, so the information transmitted is not perfect. This shows that the broiler meat market in Indonesia leads to imperfect competition. The broiler's construction meat commodity market leads to oligopsony. Government intervention is required to establish broiler meat price control policies with the primary consideration of focusing on the price leader market that will be transmitted in harmony with other regions to make the broiler meat market structure more competitive.

Growth Performance of Native Hen at the Grower Phase (Aged 6-12 weeks) After Supplemented Arginine-Lysine in Low-Protein Fed

This study aims to determine the growth performance of female Native chickens at the grower phase (6-12 Weeks) after being given arginine-lysine in low protein feed. This research was carried out from May to August 2022 at the Kampung Chicken Housing, Sion Women's Farmer Group, Sasi Village, Kefamenanu. The material used is 80 Native chickens aged 6-12 weeks with an average body weight of 400 g. The research method used is a complete randomized design. Chickens were randomly placed for 5 treatments and 4 replications, each replication consisted of 4 bird. The treatment given was P0 (control feed + 0.60% arginine + 0.52% lysine + 18% CP); P1 (control feed + 0.70% arginine + 0.61% lysine + 17% CP); P2 (control feed + 0.80% arginine + 0.70% lysine + 16% CP); P3 (control feed + 0.90% arginine + 0.79% lysine +15% CP); P4 (control feed + 1.00% arginine + 0.88% lysine + 14% CP). Variables observed were body weight gain, feed intake and feed conversion ratio. Data analysis using variance analysis and Duncan test. The results showed that the average body weight gain at treatments P0, P1, P2, P3 and P4 were 71.67±3.75; 84.69±3.55; 105.63±3.71; 105.46±7.15; and 94.38±3.50 g/bird/week, respectively. Feed intake were 277.41±15.95; 324.98±13.20; 401.13±15.10; 397.19±30.00; and 363.30±12.06 g/bird/week. Feed conversion ratio were 3.87±0.02; 3.84±0.01; 3.80±0.02; 3.77±0.03; and 3.85±0.01. Analysis of variance showed that the treatment had a significant effect on body weight gain, feed intake and feed conversion ratio of female Native chickens at the grower phase (P&lt;0.05). It was concluded that the use of the arginine-lysine increases body weight gain, feed intake and decreases feed conversion ratio of female Native chickens at the grower phase (6 – 12 weeks). The level of 0.80-0.90% arginine + 0.70-0.79% lysine can streamline the use of feed crude protein by 2-3% from 18% to 15-16% and provide maximum results on the performance of female Native chickens at the grower phase.

KERAGAMAN FENOTIPE SIFAT KUANTITATIF AYAM BURGO, AYAM HUTAN MERAH, DAN AYAM KAMPUNG DI PROVINSI BENGKULU

The importance of a good understanding of the characteristics of animal genetic resources is very necessary to serve as a reference for decision making in livestock development and breeding programs. This study aims to identify phenotype diversity of quantitative traits of burgo chickens, red jungle fowl, and kampung chickens especially in Bengkulu, and analyze the determinants of their size and shape. The research material used was 47 burgo chickens, 9 red jungle fowl (Gallus gallus spadiceus), and 18 native chickens (Gallus gallus domesticus). Samples were taken using purposive sampling technique and the chicken taken was an adult chicken in Mukomuko Regency, North Bengkulu Regency, Bengkulu Tengah Regency, Bengkulu City, and Bengkulu Selatan Regency, Bengkulu Province. Based on the results, it can be concluded that: (1) burgo chicken and red jungle chicken have quantitative traits with low level of diversity (monomorphic), conversely kampung chickens having qualitative traits with high levels of diversity (polymorphic), and (2) the determinant of the size of the burgo chicken is the length of the lower thigh (male) and the length of the third toe (female), the red jungle chicken is the length of the lower thigh (male) and the length of the upper thigh (female), and the native chicken is the length of the shank, while the determinant of the shape burgo chicken is the length of the lower thigh, red jungle chicken is the length of the third toe, and the native chicken is the length of the shank.

Application of genomic big data to analyze the genetic diversity and population structure of Korean domestic chickens

Genetic diversity analysis is crucial for maintaining and managing genetic resources. Several studies have examined the genetic diversity of Korean domestic chicken (KDC) populations using microsatellite markers, but it is difficult to capture the characteristics of the whole genome in this manner. Hence, this study analyzed the genetic diversity of several KDC populations using high-density single nucleotide polymorphism (SNP) genotype data. We examined 935 birds from 21 KDC populations, including indigenous and adapted Korean native chicken (KNC), Hyunin and Jeju KDC, and Hanhyup commercial KDC populations. A total of 212,420 SNPs of 21 KDC populations were used for calculating genetic distances and fixation index, and for ADMIXTURE analysis. As a result of the analysis, the indigenous KNC groups were genetically closer and more fixed than the other groups. Furthermore, Hyunin and Jeju KDC were similar to the indigenous KNC. In comparison, adapted KNC and Hanhyup KDC populations derived from the same original species were genetically close to each other, but had different genetic structures from the others. In conclusion, this study suggests that continuous evaluation and management are required to prevent a loss of genetic diversity in each group. Basic genetic information is provided that can be used to improve breeds quickly by utilizing the various characteristics of native chickens.