You have accessJournal of UrologyStem Cell Research1 Apr 2011180 DEVELOPMENT OF BLADDER AUGMENTATION METHOD USING SKELETAL MUSCLE-DERIVED MULTIPOTENT STEM CELL SHEET-PELLETS TRANSPLANTATION Shuichi Soeda, Tetsuro Tamaki, Akio Hoshi, Maki Masuda, Masahiro Nitta, Yukio Usui, Akira Akatsuka, and Toshiro Terachi Shuichi SoedaShuichi Soeda Isehara, Japan More articles by this author , Tetsuro TamakiTetsuro Tamaki Isehara, Japan More articles by this author , Akio HoshiAkio Hoshi Isehara, Japan More articles by this author , Maki MasudaMaki Masuda Isehara, Japan More articles by this author , Masahiro NittaMasahiro Nitta Isehara, Japan More articles by this author , Yukio UsuiYukio Usui Isehara, Japan More articles by this author , Akira AkatsukaAkira Akatsuka Isehara, Japan More articles by this author , and Toshiro TerachiToshiro Terachi Isehara, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.249AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Reconstruction of contracted bladder by illeocystoplasty using a segment of bowel has got a wide acceptance in the urological field. In this case, however, nervous control is disrupted due to the complete section of bladder-wall and replaced bowel. Thus, conservation of bladder functions such as wall contractions and sensations is difficult, whereas capacity growth is possible. Here, we tried to dilate the bladder wall associate with vascular and nerve reconstitutions using transplantation of stem cell sheet-pellets, which is formed by expanded skeletal muscle-derived multipotent stem cells (MDSCs) that have a synchronized reconstitution capacity of vascular, muscular and peripheral nervous systems. METHODS MDSCs were obtained from green fluorescent protein (GFP) transgenic mice muscles. Muscles were enzymatically dissected into several fiber-bundles, and totally cultured for 5 days. Then, cells were suspended by EDTA, and removed fibers and other debris. Remaining cells were cultured for 4 days to obtaining confluent cellular sheets. Sheets were softly suspended by EDTA, and corrected by centrifuge (MDSCs sheet-pellets). Wild-type C57/BL mice were used for recipients. Surface of the bladder-wall was sectioned from smooth muscle layers along midline legion maintaining mucosal layer, and tag ends were spread out for a transverse plane. The MDSCs sheet-pellets were pasted on the open up thin-walled bladder. Cellular engraftment and differentiation were determined by fluorescence immunohistochemistry 4 wks after transplantation. RESULTS GFP+ transplanted cells were actively engrafted on the bladder-wall and contributed to recover the thickness of the wall (Fig. 1). In addition, transplanted GFP+ cells differentiated into vascular cells (pericytes, endothelial cells, and adventitial cells) and nervous cells (Schwann cells and perineurial cells), and contribute to the reconstitution of blood vessels and peripheral nerves (arrows in Fig. 1). CONCLUSIONS Present Bladder augmentation method using one-half wall section cystoplasty and MDSCs sheet-pellets transplantation was potentially useful for the reconstruction of contracted bladder associate with native bladder functions. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e75 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Shuichi Soeda Isehara, Japan More articles by this author Tetsuro Tamaki Isehara, Japan More articles by this author Akio Hoshi Isehara, Japan More articles by this author Maki Masuda Isehara, Japan More articles by this author Masahiro Nitta Isehara, Japan More articles by this author Yukio Usui Isehara, Japan More articles by this author Akira Akatsuka Isehara, Japan More articles by this author Toshiro Terachi Isehara, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...