National Scientific Center Research Articles

Modern directions of forensic examination and criminalistic

In the conditions of war, investigators investigate hundreds of military criminal offenses. Foreign military assistance provided to Ukraine significantly improves and speeds up the investigation process.
 Today, it is extremely necessary to invite foreign criminalistic and forensic experts to help domestic investigators, prosecutors, and forensic experts in disentangling military criminal offenses and conducting forensic examinations._Regarding the state of development, certain aspects of this topic were considered in the works of such scientists as H.K. Avdeeva, M.V. Kostenko, V. Yu. Shepitko and other scientists. The purpose of the article is to consider issues related to modern areas of forensic examination and forensic analysis in the conditions of the war in Ukraine._With the beginning of a full-scale war in Ukraine, law enforcement agencies were faced with the need to conduct hundreds of types of forensic examinations of objects that were subject to individual examinations in Ukraine until 2014.
 Therefore, the help of foreign criminalistic, forensic medical experts during the investigation of military criminal offenses and conducting forensic examinations is important today. The burden on forensic experts has increased, related to conducting hundreds of DNA, weapons, etc. examinations. It is necessary to talk about the use of DNA laboratories. One of these was presented to the National Scientific Center of Forensic Examinations named after Honored Professor M.S. Bokarius in 2022. Thanks to it, forensic experts conducted hundreds of expert studies and identified the identities of the dead citizens. We should also use the innovative experience of our French colleagues, who created this DNA laboratory and have already provided two such laboratories for Ukrainian forensic experts. Another urgent problem is the interference of foreign campaigns in the activities of the domestic network of Stylnik communication. Large-scale cyberattacks on the Kyivstar network from the Russian side require investigators and prosecutors to use innovative developments in forensics and forensics. It is necessary for forensic experts to determine many circumstances and establish whether a cyber attack was carried out from the territory of the Russian Federation.

Comparative characteristics of ophthalmological symptoms of orbitocranial and orbital cavernous venous malformation

BACKGROUND: Cavernous venous malformation of the orbit, formerly known as cavernous hemangioma of the orbit, refers to vascular malformations with slow blood flow, and is a frequent lesion of the orbit in adults. The spread of cavernous venous malformation of the orbit into the cranial cavity is extremely rare.
 AIM: The aim of this study is a comparative analysis of ophthalmological manifestations of orbitocranial and orbital cavernous venous malformation.
 MATERIALS AND METHODS: The analysis was performed on 50 patients operated in N.N. Burdenko National Scientific and Practical Center for Neurosurgery from 2004 till 2023. Two groups of patients with сavernous venous malformation of the orbit were identified: group 1 — with malformation spreading into the cranial cavity, group 2 —with malformation localized in the orbit only.
 RESULTS: In 29 patients, cavernous venous malformation of the orbit spread into the cranial cavity through superior orbital fissure, inferior orbital fissure, optic canal and/or through combinations thereof; in 21 patients, the malformation was located in the orbit only. Females prevailed in both groups; the average age was 44 years. The first group included patients with the following features: visual impairments were more frequent and more prominent, oculomotor disorders caused mainly by the involvement of oculomotor and abducens nerves; optic nerve atrophy, slight proptosis. The second group was identified by more prominent proptosis, more mild visual impairments, oculomotor disorders caused by the presence of the malformation in the orbit, at the eye fundus, optic nerve head edema prevailed.
 CONCLUSIONS: Ophthalmic symptoms due to topographic and anatomical variants of a cavernous malformation.

Population and Species Composition of Rodents and Insectivores in Settlements of the West Kazakhstan Region Located in the Plague Enzootic Territory

The aim of the study was to analyze the abundance, species composition, dissemination of mouse-like rodents and other small mammals in the settlements of the West Kazakhstan Region over the past 6 years and the factors contributing to the change in the numbers in the long-term aspect.Materials and methods. We utilized the data obtained by employees of the Uralsk Plague Control Station, affiliated branch of the Republican State Enterprise “National Scientific Center of Particularly Dangerous Infections named after Masgut Aikimbaev” during a scheduled epizootiological survey of plague foci located in the West Kazakhstan Region.Results and discussion. The average annual number of rodents and insectivores in the settlements on the plague-enzootic territory of the West Kazakhstan Region was 5.1 in spring season, 8.1 in autumn, with 30.0 % occupancy of the facilities. In outbuildings, the number of rodents was three times higher than in residential buildings and amounted to 11.5 (residential buildings – 3.7). The general species composition of small mammals caught in the settlements was represented by seven species, among which the house mouse was a predominant one (97.5 %). The second place was occupied by the small shrew (1.7 %). There is a downward trend in the number of rodents in the steppe foci of plague (over 18 years – an average of 22.0 %). A slight increase in the number of animals by 2.0 % has been registered in the sandy focus.

COOMET key comparison COOMET.PR-K1.a.2018 for spectral irradiance 250 nm to 2500 nmm, final report

Main textThis report describes the Key Comparison COOMET.R-K1.a.2018 for spectral irradiance in the wavelength range of 250 nm to 2500 nm, conducted by the Regional Metrology Organization "Euro-Asian Cooperation of National Metrological Institutions" (COOMET). The comparison was, in fact, a combination of three bilateral comparisons with the same link/pilot laboratory - the All-Russian Research Institute for Optical and Physical Measurements (VNIIOFI), the Russian Federation. The non-link laboratories were the Belarusian State Institute of Metrology (BelGIM), Belarus, the National Scientific Centre "Institute of Metrology" (NSC IM), Ukraine, and the National Metrology Institute of Türkiye (TÜBİTAK UME), Türkiye.The aim of the comparison was to link the BelGIM, NSC IM and TÜBİTAK UME measurement results to the CIPM key comparison CCPR-K1.a.2017, conducted by the Consultative Committee for Photometry and Radiometry (CCPR).Tungsten quartz halogen lamps of the FEL type (1000 W) were used as artefacts. Each participant used its own set of lamps. The sequence of measurements was participant - pilot - participant. The pilot measurements of the described here COOMET comparison were performed in a short time (within three months) after completing the measurements of CCPR-K1.a.2017, where VNIIOFI also was a pilot, which provided a high quality link.NSC IM performed measurements in the full spectral range, from 250 nm to 2500 nm, while BelGIM and TÜBİTAK UME managed to cover the range from 250 nm to 1100 nm only.The analysis of the comparison was performed following the approach described in the Appendix A of the Guidelines for CCPR and RMO Bilateral Key Comparisons (CCPR-G5). Differences from KCRV of BelGIM and TÜBİTAK UME, for the most of the measured wavelengths, do not exceed 2% and are within the expanded uncertainties. For NSC IM the differences from KCRV are within or comparable with the uncertainties in the IR range, from 900 nm to 2500 nm, but become larger with decreasing wavelength in the visible and UV reaching 9% at 250 nm.To reach the main text of this paper, click on Final Report. Note that this text is that which appears in Appendix B of the BIPM key comparison database https://www.bipm.org/kcdb/.The final report has been peer-reviewed and approved for publication by the CCPR, according to the provisions of the CIPM Mutual Recognition Arrangement (CIPM MRA).

APPLICATION OF ETIOLATION OF GRAPE EXPLANTS IN VITRO CULTURE

Micropropagation of grapes is used to obtain original and certified planting material for planting mother plants. The decrease in the efficiency of microclonal reproduction is associated with difficulties at the stages of introducing explants into in vitro culture and rooting. The method of etiolation is simple to use and allows you to prevent phenolic oxidation of explants, has a positive effect on increasing the length of shoots and the number of roots. The purpose of the research was to determine the effectiveness of etiolation at the stages of introducing explants and rooting microcuttings in in vitro culture. The work was carried out in the laboratory of in vitro grape culture of the department of grape nursery, propagation and biotechnology of the National Scientific Centre «V.Ye. Tairov Institute of Viticulture and Winemaking» NAAS of Ukraine according to the generally accepted method on table, technical and grafted varieties of grapes. Etiolation was applied in two stages: introduction of initial grape explants into in vitro culture (for 7, 15, and 25 days) and rooting of grape microcuttings (for 7 and 15 days). The survival and proliferation of explants, the main biometric indicators of growth and development of microclones were determined. It was established that at the stage of introduction of initial explants of grapes into in vitro culture, the use of etiolation for 15 days, and at the stage of micropropagation - for 7 days, had a positive effect on rooting, proliferation of axillary buds and further development of explants and microcuttings. The height of the stem of the experimental microclones was 0.6-2.6 cm higher, and the number of leaves was 0.4-0.9 more than that of the control plants. Also, etiolation had a positive effect on the increase in the length and biomass of roots in microcuttings. In the future, it is planned to analyze changes in the in vitro reproduction coefficient of various grape varieties under the influence of etiolation.

Optimal tactics for surgical revascularisation in diabetic angiopathy of the lower limbs.

Aim: This study aims to compare the efficacy of conservative treatment methods versus advanced surgical interventions, including revascularising automyelotransplantation and stem cell therapy, in improving vascular patency and the quality of life in patients with diabetic angiopathy. Materials and Methods: The research analyzed 68 patients with angiopathies under medical supervision from January 2007 to December 2017 at the National Scientific Center of Surgery named after A.N. Syzganov. Participants, aged 4 to 49, were divided into two groups based on angiographic blood flow characteristics: one with accelerated and another with delayed blood flow. A comprehensive participant selection process was implemented to ensure a representative sample. Sensitivity analysis was conducted to validate the findings' robustness. Results: The main group demonstrated notable success in limb salvage, with 90.9% avoiding high limb amputation post-revascularising automyelotransplantation. Moreover, 16.7% of patients experienced healing of trophic ulcers and toe necrosis. The use of stem cells from adipose tissue and fetal tissue progenitor cells showed promising results in reducing pain and increasing pain-free walking distance, alongside the formation of collateral vascular networks. Conclusions: The study concludes that advanced surgical interventions and stem cell therapies significantly enhance treatment outcomes in patients with diabetic angiopathy compared to conventional conservative treatments. These findings highlight the potential of personalized and innovative approaches in managing vascular complications associated with diabetes, offering new avenues for reducing disability and improving patient quality of life. Future research should focus on further refining these therapeutic strategies and exploring their integration into clinical practice.

Experience of liver transplantation in National Scientific Center for Surgery Named after A.N. Syzganov

Experience of liver transplantation in National Scientific Center for Surgery Named after A.N. Syzganov

Computed tomography-guided intraoperative navigation in children with congenital scoliosis versus freehand/fluoroscopy methods

BACKGROUND: The choice of techniques for the treatment of children with congenital spinal deformities remains one of the most significant problems of spinal surgery. This topic is relevant given the peculiarities of the disease course, severity and rigidity of deformities, their steady and rapid progression, formation of compensatory curvature, and a significant decrease in the quality and life expectancy of patients.
 AIM: To compare screw misposition, adverse outcomes, intraoperative blood loss, and time required for pedicle screw placement with further deformity correction under computed tomography (CT) guidance with intraoperative navigation versus fluoroscopy.
 MATERIALS AND METHODS: This single-center, prospective comparative study was conducted from 2019 to 2022 at the National Scientific Center of Traumatology and Orthopedics named after academician N.D. Batpenov. Patient demographics and surgical outcomes were obtained from the medical records. All patients underwent a comprehensive clinical and radiological examination before surgery, after surgery, and at the stages of dynamic observation. Data of patients with congenital malformations of the spine were analyzed. The study involved 42 patients aged 318 years with congenital kyphoscoliosis of the thoracic and/or lumbar spine. The patients were divided into two groups according to the method of surgical correction used: the O-arm navigation group and the C-arm group.
 RESULTS: Data of patients who underwent surgery for congenital scoliosis of the spine were analyzed. The patients were divided into the O-arm navigation group, which included patients who underwent surgery using the O-arm mobile intraoperative CT with the seventh-generation Stealth Station navigation system in combination with intraoperative neuromonitoring, and the C-arm group, which included patients who underwent surgery under the control of the intraoperative C-arm. In both groups, 364 screws were placed, of which 189 screws were placed under neuronavigation, and 175 screws were placed using the C-arm. The effectiveness of the intraoperative neuronavigation system in combination with neuromonitoring showed 97.11% correct placement (grades A and B) of pedicle screws. The use of an intraoperative C-arm showed 89.63% (grades A and B) correctness. The proportion of misplaced screws corresponding to GertzbeinRobbins classes CE was higher in the C-arm group (10.37%) than in the navigation group (1.49%) (p 0.005). No severe neurological disorders, postoperative infection, or adverse clinical outcomes were observed in both groups.
 CONCLUSIONS: The installation of pedicle screws using CT-guided navigation (O-arm) did not prolong the operation time, did not increase blood loss, and reduced the risk of screw mispositioning compared with freehand and fluoroscopy pedicle screw placement.

Features of the Course of Atrial Fibrillation in Patients with the History of COVID-19: Impact on the Possibility and Tactics of Restoring Sinus Rhythm

Introduction. Atrial fibrillation (AF) is a potentially life-threatening complication of infection. In general, AF has a high prevalence in elderly population with cardiovascular risk factors and comorbidities. Thus, the presence of AF correlates with adverse outcomes in patients with previous coronavirus disease (COVID-19) which deserves increased attention and should be appropriately treated to prevent adverse outcomes.
 The aim. To analyze the prevalence and clinical course of AF in hospitalized patients after COVID-19 to determine approaches to restoring sinus rhythm.
 Materials and methods. The study included 179 patients with various types of heart rhythm and conduction disorders who suffered from COVID-19 and were hospitalized to the Department of Clinical Arrhythmology and Electrophysiology of the National Scientific Center “The M.D. Strazhesko Institute of Cardiology, Clinical and Regenerative Medicine of the NAMS of Ukraine” from 09/20/2020 to 12/21/2021. 116 patients were hospitalized with AF who suffered from coronavirus infection between 1 and 12 months ago (5.1 ± 0.2 months on average). Other heart rhythm and conduction disorders were the cause of hospitalization for 63 patients (35.2%). The first group was formed by 36 people (31%) in whom AF occurred after coronavirus infection. The second group included 25 patients in whom the form of AF changed, namely: there was a transition from paroxysmal form of AF to persistent AF, or persistent AF to a permanent form of AF. The third group included 55 patients in whom the form of AF did not change. However, in the third group, two subgroups were formed: 3A consisting of 35 patients, in whom, although the form of AF did not change, the frequency or duration of paroxysms increased, and 3B, which included 20 patients without significant changes in the course of AF. As a control group, 49 patients with AF without a history of coronavirus infection were examined.
 Results. In most patients (65%), the reason for hospitalization was AF. The first recorded paroxysms of this arrhythmia were recorded in 31% of patients 2 months after the coronavirus infection. In this group, more than half of the cases (58.4%) were patients with persistent AF, and 8.3% of those hospitalized failed to restore sinus rhythm. In those who had AF before COVID-19, 75% of its course worsened: the frequency or duration of paroxysms increased. The persistent form of AF was dominant and occurred after a previous infection in 58% of cases. In patients who had AF before COVID-19, its course worsened (in 76% of the examined persons) after the infection. Patients with a history of coronavirus infection had more frequent use of drug therapy, which is probably due to the delayed time of hospitalization to the clinic from the onset of the paroxysm.
 Conclusion. AF is the most common arrhythmia and has a worse course in hospitalized patients after coronavirus infection.

THE PECULIARITIES IN THE FORMATION OF GUNSHOT WOUNDS CHANNELS CAUSED BY 7.62 MM EXPANSIVE «HPBT» AND «SP» TYPE BULLETS

The aim of the study – to study the peculiarities of wound channel formation, causedby 7.62 mm «HPBT» and «SP» bullets, by means of experimental methods of forensicballistics with the use of human biological tissue simulators.Materials and methods. Experimental investigations were carried out under conditionsof the ballistic route of the National Scientific Center «Hon. Prof. M. S. Bokarius ForensicScience Institute». To determine the velocities of the fired bullets, the optoelectronicmeasuring complexes IBX-731.4 were used. Ballistic plasticine «Beschussmasse,6287156» was used as a simulator of human body tissues. The experimental shooting wascarried out with a TS.M308 model .308 «Winchester» sniper rifle. For the experimentalshooting 7.62 × 51 mm (.308 «Winchester») cartridges were used, which were filled with«SP» and «HPBT» bullets.Results. In the course of experimental shooting, the peculiarities of the formation ofdamage channels caused by 7.62 mm «SP» and «HPBT» bullets were determined. Basedon the results of experimental studies, it has been found that the bullets of these types ofthe same caliber and practically the same mass are able to cause different amounts ofbodily injury, which is primarily due to the peculiarities of the design of the bullets. SPtype bullets with a bare core cause more severe bodily injuries, they are less subject tofragmentation and in most cases retain a relatively heavy bottom part. As a consequence,such bullets are able to cause longer wound channels than other types of expansivebullets. HPBT-type bullets can also cause sufficiently large amounts of bodily injury,but because of their design, they are more prone to fragmentation when hitting thehuman body. In the course of the research it has been established that there are severalperipheral (secondary) channels in the area of the main wound channel. They are formeddue to destruction of the bullet and movement of its separate fragments away from themain direction, which leads to complication of diagnostics and further treatment of suchgunshot wounds.Conclusions. The use of 7.62 × 51 mm (.308 «Winchester») expansive «SP» and «HPBT»type bullets in the course of combat operations for firing modern sniper systems resultsto severe bodily injuries. Wounds caused by such bullets are characterized by significantin volume zones of direct traumatic necrosis (contusion), which significantly extend deepinto the tissues and structures of the human body and form even larger in size zonesof conditional necrosis (contusion) and morpho- functional changes. The formationnature of both the main wound channel and the peripheral wound channels, caused byfragments of bullet structural elements, depends on the velocity, design features andposition (projection) of the bullet at the moment of impact. Changes in biological tissuesin the area of wounds caused by these types of expansive bullets have rather complexarchitectonics, which significantly complicates the issue of clear delimitation of gunshotwound zones and adequate surgical intervention. The used investigation methods of thestriking properties of expansive bullets are suitable for predicting the severity and volumeof the caused injuries, as well as for determining the individual characteristic complex ofgunshot wounds signs caused by such bullets.

BIODIVERSITY AND BIORESOURCES OF DEEP-SEA ECOSYSTEMS OF THE NORTHWESTERN PACIFIC

Modern problems of the investigation of marine biodiversity and bio-resources and their inventory in the deep-sea of the World Ocean are considered. The discussion of these problems is also based on the data of aseries of deep-water marine expeditions of the National Scientific Center of Marine Biology of the Far Eastern Branch of the Russian Academy of Sciences (NSCMB FEB RAS) in the deepest areas of the Far Eastern Seas and adjacent waters of the North-West Pacific. New results of a series of the deep-sea expeditions within the special RAS Project “Fundamental problems of investigation and conservation of the deep-sea ecosystems in the potentially ore-reach areas in the North-West Pacific” are briefly introduced and discussed (project of RAS № 3.1902.21.0012). Several “unique” deep-sea ecosystems found in the ore bearing sites and the active fishing areas in the North-West Pacific are described. Some safety opportunities for these “unique” ecosystems and possible limitations in the use of dangerous mining and fishing techniques disturbing deep-sea landscapes are considered, including various conservation statuses.

THE BENEFITS OF DUPLEX SCANNING OF EXTRACRANIAL CAROTID PATHOLOGIES FOR RISK STRATIFICATION OF ISCHEMIC STROKE

Abstract Introduction: Recent retrospective cohort study demonstrated the benefit of duplex scanning in the examination of the extracranial carotid artery pathologies for preventing cerebrovascular disorders. The aim of this study: was to evaluate the clinical and demographical characteristics and outcome in patients treated at National Scientific Center of Surgery, after detecting extracranial carotid artery stenosis ≥70%. Material and methods: A retrospective cohort study was conducted; 204 patients were included. Results: The most significant risk factors were: ischemic heart diseases – 95.6%, arterial hypertension – 80.1%, chronic heart failure – 83.8%, cholesterol level above the norm of 5.2 mmol/L-39.2%; 30.4% of patients had a history of postinfarction cardiosclerosis. There were a significant statistically difference between the prevalence of extracranial carotid artery stenosis depending on the risk factors (blood cholesterol levels, a clinical history of chronic heart failure, and acute cerebrovascular diseases p=0.048, p=0.003, p=0.048 respectively (p>0.05)). Conclusion: In summary, we conclude that surgery should be considered, and duplex ultrasound plays a crucial role in assessing the severity of carotid stenosis in atherothrombotic stroke patients with severe external carotid stenosis. These data indicate that physicians, should be aware of the usefulness of carotid duplex ultrasound scanning in stratifying the risk of cerebral and cardiovascular diseases based on various aspects.

Metrological traceability of the results of testing for electromagnetic compatibility in accordance with the NATO standards

Ensuring the electromagnetic compatibility of special-purpose equipment used in a complex saturated radio frequency environment requires testing in accordance with procedures that guarantee a valid verification of its immunity to external electromagnetic fields. The paper presents the national measurement standards and equipment used at the National Scientific Centre “Institute of Metrology” for the NRE02, NRE03 and NRS02 testing in accordance with the AESTR-500 standard. It is shown how metrological traceability to the SI units of the measurement results is ensured during tests. The paper presents the results of evaluating the uncertainty of the results of measurements of the electromagnetic field strength using the NARDA-550 meter for the frequency ranges of 10 kHz – 1 GHz when using the GTEM chamber, and for the frequency ranges of 1-43 GHz when using the anechoic chamber. The studies have proven that the measurement uncertainties, when calibrating the antenna in the anechoic chamber, and the uneven distribution of the electromagnetic field in the GTEM chamber are the most critical. The obtained results of the uncertainty evaluation of the NARDA-550 calibration coefficient are used for high-precision measurements of radio frequency signals both at emission and being exposed to electromagnetic fields when the disturbances have limit values as regards the provisions of these standards. These results with a discrepancy of no more than 0.05 dB confirm the characteristics of the device provided by the manufacturer. It is shown that the set of national measurement standards and test equipment of the NSC “Institute of Metrology” allows testing special equipment for electromagnetic compatibility according to the NATO standards with the required accuracy.

Creation and a breeding-genetic study of pollinator lines - sterility fixers and their CMS-analogues in fodder beet breeding for heterosis

Purpose. To create pollinator lines - sterility fixers and their cytoplasmic male sterility (CMS)-analogues and to study their breeding and genetic features for the development of parental components of CMS-based one-sprout fodder beet hybrids.
 Materials and Methods. The study was carried out at the Tobacco Research Station of the National Scientific Center "IA NAAS" (Cherkaska Oblast, Uman) in 2013–2021. Four diploid one-sprout fodder beet populations of hybrid origin were taken as the starting material. Fodder beet lines were created by inbreeding. Analyzing crossings were carried out under paired coarse calico bags. Breeding accessions were tested in accordance with a method developed by scientists of the Institute of Bioenergy Crops and Sugar Beet of NAAS.
 Results and Discussion. The results indicate a fairly high frequency of pollinators - sterility fixers among the one-sprout fodder beet hybrids bred in Uman. The mean across all the initial accessions was 7.5%. One-sprout hybrids KT-7105 2хmm and KZ-1507 2хmm demonstrated the highest incidence Owen type pollinators - sterility fixers. A high level of pollen sterility was intrinsic to the CMS analogues of the O-type lines derived from the initial accessions IM-3319 2хmm and KZ-1507 2хmm (99.1% and 97.0%, respectively). The highest percentage of one-sprout seeds was recorded for the CMS analogues of the O-type lines derived from hybrid accessions KZ-1507 2хmm and KT-7105 2хmm. Fifteen best O-type lines have been selected for further breeding to obtain parental components of CMS-based experimental hybrids.
 Analysis showed that the beetroot yields of the majority of the CMS fodder beet lines were lower than that of the check variety. CMS IM-3319/16, CMS IM-3319/34 and CMS IM-3319/49 were the only lines that yielded at the level of the check variety (80.9–83.6 t/ha), which is attributed primarily to their genetic origin. As to the dry matter content in beetroots, only lines CMS ST-4514/27 and CMS ST-4514/39 significantly exceeded the check variety; the values of the others were similar to that of the check variety. In terms of the dry matter output, except for CMS KT-7105/7, CMS IM-3319/34 and CMS IM-3319/49, most lines were inferior to the check variety.
 Conclusions. We found that the portion of pollinators - CMS fixers in one-sprout populations of fodder beets of different genetic origins was 3.7–11.3%. Fifteen combinable O-type lines with pollen sterility fixing of 96.2–100% and percentage of one-sprout seeds of 96.4–99.3% have been created. Their CMS analogues have been obtained. The created breeding accessions are a valuable starting material for the selection of parental components and generation of highly productive CMS-based one-sprout fodder beet hybrids

Рецензія на монографію «досвід військових формувань у ліквідації наслідків аварії на чорнобильській атомній електростанції через призму сучасних радіаційних загроз (до 35 роковин аварії на чорнобильській атомній електростанції)»

The Monograph is the result of scientific and practical cooperation of the Ministry of Defense of Ukraine, the General Staff of the Armed Forces of Ukraine, the Command of the Medical Forces, the Ukrainian Military Medical Academy, the SE “L.I. Medveds Research Center of Preventive Toxicology, Food and Chemical Safety of the Ministry of Health of Ukraine”, State University “Na-tional Scientific Center of Radiation Medicine of the National Academy of Sci-ences of Ukraine” in the field of radioprotection, namely - prevention and elimi-nation of radiation exposure, organization of radiation, chemical and biological protection of forces during elimination of the consequences of a radiation acci-dent. The main goal of the Monograph is the analysis of the practical aspects of the cooperation of servicemen of the Armed Forces, representatives of power minis-tries and agencies, and the health care system on the elimination of the conse-quences of the accident at the Chernobyl nuclear power plant. The authors note that the experience gained was the basis for the development of scientific recom-mendations for improving radioprotection, taking into account the radiation ef-fect on human health and the environment. Special attention is paid in the Monograph to the study of the key principles of prevention and elimination of radiation exposure, the peculiarities of the or-ganization of radiation, chemical and biological protection of forces during the elimination of the consequences of a radiation accident. Undoubtedly, the Monograph is a useful source of information for doctors of various specialties, scientific specialists, students of the Ukrainian Military Medi-cal Academy, students of higher medical schools and educational institutions of postgraduate education, and military specialists in the organization of radiation, chemical and biological protection of forces.

Insufficiently studied minor viral infections in livestock of Ukraine

For the first time in Ukraine the presence of genetic material of bovine immunodeficiency virus and bovine foamy virus in cattle on Ukrainian farms was detected by scientists of the Laboratory of Leukemia Study and the Laboratory of Molecular Diagnostics of the National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’. The associative nature of animal infection with leukemia, immunodeficiency and spumavirus pathogens is recorded. In the future, it is planned to study the properties of pathogens, adaptation to homologous cell cultures and accumulation of viral material in order to develop domestic means of serological diagnosis of immunodeficiency and spumavirus infection

ШЕЙНАЯ ЭКТОПИЯ ТИМУСА У ДЕТЕЙ

Thymus ectopia is a rare solid neck lesion in children. The location of the formation along the path of thymus migration (thymopharyngeal duct), the echo pattern and the intensity of the MR signal, which is identical to the normally developed thymus, help in making a confident diagnosis. Materials and methods of the research: 14 pediatric patients were treated against the cervical thymus ectopia in 2018-2022 in the Russian Children's Clinical Hospital with the Pirogov Russian National Research Medical University of the Ministry of Healthcare of Russia and the National Scientific and Practical Center for Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev (both located in Moscow, Russia). The diagnosis was set only after the histological examination results in all cases. A differential diagnosis was made with other cystic and solid formations, taking into account its location and distribution according to ultrasound, computed tomography, and magnetic resonance imaging in each case. Results: stable, relapse-free treatment result was achieved after more than a year of follow-up in all cases. The correct diagnosis wasn’t set in every case until the biopsy results due to the rarity and diverse clinical and radiological picture of the disease. Conclusion: cervical thymus ectopia is a benign and relatively rare condition in children that is rarely diagnosed preoperatively by the visualization only. The conservative management may be appropriate in some cases when the thymus is in the chest cavity only, but surgical correction is more often needed in cases with the neck deforming formations.

ФИЗИЧЕСКОЕ И ДВИГАТЕЛЬНОЕ РАЗВИТИЕ ДЕТЕЙ, ЛЕЧИВШИХСЯ ОТ ОНКОЛОГИЧЕСКИХ ЗАБОЛЕВАНИЙ, КАК ФАКТОР, ВЛИЯЮЩИЙ НА СВЯЗАННОЕ СО ЗДОРОВЬЕМ КАЧЕСТВО ЖИЗНИ

Article represents the results of the research undertaken to prove the relationship between the physical conditions of patients treated for oncological diseases (OD) and the decrease in their health-related quality of life (HRQOL). Materials and methods used: 1564 children aged 6 to 18 y/o who have undergone rehabilitation at the “Russkoye pole” Clinical Rehabilitation Research Center for patients in remission with the National Scientific and Practical Center for Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev located in Grishenki village, Chekhovsky District, Moscow Oblast, Russia, after the complex treatment for OD from 2018 to 2021 participated in the research, of which 864 boys and 700 girls. The average age of the patients was 10.6+/-3.4 y/o, the average remission period was 5.4+/-2.7 years. The comparison group consisted of healthy siblings aged 6 to 18 y/o who stayed at the same facility and during the same period, a total of 238, of which 111 boys and 127 girls. The average age of the examined siblings was 10.2+/-2.4 y/o. The indicators of physical condition were tested as follows: anthropometry, strength endurance of muscles, tolerance to physical loads, development of motor skills. The PedsQL questionnaire was used for the HRQOL assessment. In order to build the mathematical models and to evaluate the statistically significant influence of factors, the classical linear regression models were used. The analysis of the relationship between groups of variables was carried out with the canonical correlation analysis. Comparison of children treated for OD and healthy children was carried out using the Welch's t-test. Results: all of the HRQOL indicators in all gender and age subgroups in children with OD were statistically significantly lower compared to healthy siblings. The most significant differences were obtained in the scale of physical functioning (p=0.001). A direct moderate relationship was found between HRQOL and a factor indicator for the physical condition (rcc=0.53), a direct moderate-weak relationship with a generalized measure of strength endurance (rcc=0.315) and a direct moderate relationship with motor skills (rcc=0.5). It was also found that a lag in the formation of basic motor skills had the greatest negative impact on the HRQOL of children treated for OD. Conclusion: the results of the research do confirm the role of physical condition in improving the patients’ quality of life. Continued research would allow developing a new tool for assessment of the effectiveness of physical rehabilitation for patients in this category.

OUR EXPERIENCE OF LAPAROSCOPIC PANCREATODUODENECTOMY IN TUMORS OF THE PERIAMPULLARY ZONE

The purpose of research is to determine the feasibility of laparoscopic pancreatoduodenectomy in tumors of the periampullary zone. Material and methods. In the period from 2016 to 2022 at the Syzganov National Scientific Center of Surgery, 193 patients underwent PD with a diagnosis of a tumor of the periampullary zone. Of these, 6 patients were performed laparoscopically. All patients who underwent LPD were female. The age of the patients ranged from 15 to 77 years (average age – 55,7 years). Results. In 4 (66.7%) patients, cancer of Ampulla of Vater was detected, in 2 (33.3%) pancreatic head cancer. According to the final histology data, adenocarcinoma and the degree of differentiation G2 were detected in all cases. Data on the histology and size of the tumor are summarized in Table 1. The average age of patients was 55.7 years. All patients had a clinic of mechanical jaundice before surgery; the average levels of bilirubin in the blood were 121.3 mmol/l. Accordingly, all patients underwent drainage of the biliary tract. Of these, 5 (83.3%) patients underwent percutaneous stenting and 1 (16.7%) endobiliary stenting. Conclusion. Thus, we presented our initial experience of performing laparoscopic PD. Our results shows the feasibility of laparoscopic PD safely and radically for tumors of the periampullary zone in certain cases. The accumulation of experience in such interventions leads to an improvement in immediate results and a reduction in postoperative complications, the operative time.

Endothelial dysfunction in the pathogenesis of arterial hypertension: new diagnostic methods

ENDOTHELIAL DYSFUNCTION IN THE PATHOGENESIS OF ARTERIAL HYPERTENSION: NEW DIAGNOSTIC METHODS Tetiana V. Talaieva State Institution «National Scientific Center «M. D. Strazhesko Institute of Cardiology, Clinical and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine», 5, Svyatoslava Khorobroho Str., Kyiv, Ukraine 03151 Introduction. Arterial hypertension (AH) remains the most widespread disease of the circulatory system, as well as one of the leading risk factors for the development of cardiovascular diseases. Recent studies indicate that endothelial dysfunction may play a key role in the pathogenesis of hypertension. Endothelial dysfunction is associated with damage and accelerated apoptosis of endothelial cells (ECs), and quite often these changes occur before morphological and clinical signs of the disease appear. The researches of the last decades established that the most important properties of the endothelium - restoration and preservation of structural and functional integrity and its reparative activity are directly related to circulating endothelial progenitor cells - precursor cells of endotheliocytes (ECCs). The use of various markers for the determination of ECCs in circulating blood, the determination of the content of exfoliated endothelial cells and the reserve function of the bone marrow (ability to produce ECCs) makes it possible to assess the function of the endothelium and the risk of the development and progression of cardiovascular diseases. Purpose: using the flow cytometry method to evaluate the possibility of determining the content of ECCs in the blood, desquamated ECs and the reserve function of the bone marrow (ability to produce ECCs) as markers of endothelial dysfunction. Materials and methods. 153 patients with AH were included in the study. All patients underwent complaint registration, history taking, general clinical examination, including office blood pressure measurement and daily blood pressure monitoring, physical examination, brachial artery compression test to assess endothelium-dependent vasodilatation. The number of ECCs of peripheral blood was determined by the method of flow cytometry with the help of reagents for the determination of differentiation clusters CD34, CD45, CD31, CD133 manufactured by "Beckman Coulter Inc.". In the conditions of the test with dosed physical load, the blood content of ECCs was determined on a bicycle ergometer before and after 60 minutes. after completing the test on 55 patients with hypertension. The results. In the studies, complex determination of various markers on the surface of the ECCs was used. At baseline, the number of ECCs (CD34+/CD45-/+) was 22 % lower in patients with hypertension, and 28 % lower in patients with resistant hypertension than in practically healthy donors (р < 0.05). The number of ECCs (CD133+ CD31+ CD45-/+) in patients with hypertension was 25 % less than in the norm. The number of desquamated cells exceeded the norm by 152 % (р < 0.001). In patients with hypertension, there was a decrease in the reserve function of the bone marrow to produce ECCs in response to ischemia caused by stress. The obtained data are confirmed by the results of the brachial artery compression test. Based on the results of the analysis in the groups with controlled and resistant hypertension, it was found that the last index of EDVD was 25 % lower than in the group of patients with hypertension that is well controlled (р < 0.05). The use of standard therapy for 12 weeks was accompanied by an increase in the number of ECCs in patients, which indicated the restoration of endothelial function after the treatment. Conclusions. In patients with hypertension, a decrease in the content of ECCs in the blood was noted. The resistant course of hypertension is associated with greater manifestations of endothelial dysfunction. Determination of ECCs using the flow cytometry method provides important additional information about endothelial dysfunction as a risk factor for the development and progression of hypertension, and can also be used to assess the effectiveness of antihypertensive therapy. Keywords: arterial hypertension, endothelium, dysfunction, endothelial progenitor cells.