Abstract Urban humans and biodiversity-related concepts are interacting with each other in many negative and positive ways. The biodiversity provides a wide array of provision and cultural-ecological services to urban residents, but it is being overexploited to the point of crisis. The crisis is largely driven by the expanding illegal wildlife trade in developing countries with a high urbanization rate and biodiversity level like Vietnam. While supply-side measures are ineffective in reducing biodiversity loss, researchers have suggested demand-side measures as supplements, such as social marketing campaigns and law enforcement in urban areas. Moreover, urban residents are also potential visitors to urban public parks and national parks, which helps generate finance for biodiversity preservation and conservation in those places. Understanding how urban residents' perceptions towards biodiversity and biodiversity-related behaviors can help improve the effectiveness of conservation efforts and sustainable urban development. Thus, this article presents a dataset of 535 urban residents' wildlife consumption behaviors, multifaceted perceptions and interactions with biodiversity-related concepts, and nature-based recreation demand. The dataset is constructed with six major categories: 1) wildlife product consumption, 2) general biodiversity perceptions, 3) biodiversity at home and neighborhood, 4) public park visitation and motivations, 5) national park visitation and motivations, and 6) socio-demographic profiles. These resources are expected to support researchers in enriching the lax literature regarding the role of urban residents in biodiversity conservation and preservation, and help policymakers to find insights for building up an “eco-surplus culture” among urban residents through effective public communication and policymaking.