Background: The microbial population of wound isolates remains a public health concern because of its role in spread of infections. The microorganisms present in wound swab of patients have been reviewed in order to ascertain the presence of emerging and re -emerging pathogens in hospital environment. These microorganisms are generated from nosocomial and opportunistic origin as well as immune compromised individuals. The purpose of this study is to determine the occurrence of microorganisms in wounds of patient’s attending clinic in National Orthopedic Hospital Enugu, Nigeria. Methodology: A cross sectional study of 230 wound patients was selected using convenient sampling technique. Conventional methods and 16SrRNA sequencing was used for characterization of some organisms that could not be identified in the conventional methods. Questionnaire structured from literature review was also deployed for demographic studies obtained from consented participants. Results The frequency distribution of wound isolates showed an increase in Pseudomonas aeruginosa 7(10%) and Klebsiella pneumonia 6(8.6%) respectively. Mixed growths had a notable increase of 42(18.3%),while culture with no bacteria growth had 37(16.1%). Staphylococcus aureus was prevalent with 32(13.9%), Staphylococcus epidermis 15(6.5%) and the least isolates had 1(1.4%), Male gender had the highest wound isolates of one hundred and fourty one (141) with meanSD 21.11 and median of 19.00 while the female had 88 with meanSD 20.3113.457, median of 16.50. The socio demographic data findings for participant’s age were higher in the range of 21-40yrs (94) and 41-60yr (74) while the least was >80 years (5). The mean age of the 230 participantswas 43.15±17.284yrs.Wound type had a notable increase in accident cases (63)27.4%, surgical cases (44)19.1% and lowest was laceration cases (2) 0.9%, The most prevalent population of wound cases according to occupational status was observed in artisans (148) 64.3%, students (33)14.3%, civil servants (29)12.6%, least population was the retired (20)8.7%. The portion of wound site was highest in the leg region (174), trunk (22) and the hand (12). Conclusion: it is advocated that proper measures such as adequate sanitation, personal hygiene, sterilization of hospital equipment and environment should be beefed up to prevent the spread of these microbes to the public.