The 8th African Conference on One Health and Biosecurity with the theme Strengthening Health Security and Mitigating Biological Threats in Africa was held Wednesday, 2nd November- Friday 4th November, 2022. The 8th edition of the annual conference was organized by the Global Emerging Pathogens Treatment Consortium (GET Africa) with the support of Lagos State Ministry of Health, and in partnership with major non-state institutions across the World. The conference focused on ways of improving health security in the African Continent and addressing emerging biological threats. The 3-day conference present a unique forum to raise National, Regional and Continental awareness and engage in deep introspection and robust interactions on existing health security measures and how to strengthen them, as the first urgent step toward mitigation of emerging biological threats in Africa. The conference, attended by professionals and stakeholders across the various strata of the health and allied sectors of the society, received presentations from resource persons in the healthcare sector and related fields. The following observations and recommendations emerged from exhaustive deliberations