Space Narrowing Research Articles

Research on the drone endoscopic system for fusion reactors

This paper proposes an air-ground separation drone endoscopic system (DES) design scheme for the fusion reactor internal inspection task. A foldable rotor drone with vertical takeoff and landing capability is used as the main body of DES. The nest platform is designed separately from the drone to reduce the weight. Meanwhile, the nest platform can be installed with a safety cable and 2D code to realize emergency rescue and precise take-off and landing. DES adds 3D lidar sensors for 360° stereo sensing, realizing real-time positioning in dark or even lightless environments in vacuum chambers. The first-generation DES prototype is developed, and the flight test is performed inside the PF6 superconducting coil shell by fusing 3D lidar point cloud data with visual camera image data. The results show that the position error of DES is stabilized within ±0.05 m during the hovering phase. The error is mainly due to the narrow space inside the PF6 coil shell, which produces a perturbed flow field on the DES. In general, the DES accomplished circumferential autonomous flight control and 3D point cloud data acquisition in a confined space, proving the feasibility of drone inspection in vacuum chambers.

Speleothem-Inspired Copper/Nickel Interfaces for Enhanced Liquid-Vapor Transport by Marangoni and Soret Effects.

Geological formations have superior wickability and support the absorption of water and oils into narrow spaces of Earth's crust without external assistance. In this study, we present speleothem inspired heterogeneous porous and wicked copper (Cu)/nickel (Ni) interfaces for enhanced nucleate boiling of water/ethanol mixtures for energy-efficient separation processes. The incorporation of Ni strands within the copper particle matrix significantly enhanced heat transfer. Compared to plain copper, the Cu/Ni speleothem surfaces exhibited a 61% increase in the heat transfer coefficient for water/ethanol mixtures and a 332% increase for water, with a 58% faster onset of nucleate boiling. This enhancement was attributed to Marangoni and Soret effects at the Cu/Ni interfaces, driven by surface tension and concentration gradients. Furthermore, the synergistic wicking action of the Ni strands facilitated rewetting of the surface, replenishing liquid to the porous nucleation sites and preventing surface dry-out, thereby improving the overall heat transfer performance.

Electrowriting patterns and electric field harness directional cell migration for skin wound healing

Directional cell migration is a crucial step in wound healing, influenced by electrical and topographic stimulations. However, the underlying mechanism and the combined effects of these two factors on cell migration remain unclear. This study explores cell migration under various combinations of guided straight line (SL) spacing, conductivity, and the relative direction of electric field (EF) and SL. Electrowriting is employed to fabricate conductive (multiwalled carbon nanotube/polycaprolactone (PCL)) and nonconductive (PCL) SL, with narrow (50 μm) and wide (400 μm) spacing that controls the topographic stimulation strength. Results show that various combinations of electrical and topographic stimulation yield significantly distinct effects on cell migration direction and speed; cells migrate fastest with the most directivity in the case of conductive, narrow-spacing SL parallel to EF. A physical model based on intercellular interactions is developed to capture the underlying mechanism of cell migration under SL and EF stimulations, in agreement with experimental observations. In vivo skin wound healing assay further confirmed that the combination of EF (1 V cm−1) and parallelly aligned conductive fibers accelerated the wound healing process. This study presents a promising approach to direct cell migration and enhance wound healing by optimizing synergistic electrical and topographic stimulations.

Boiling of Coolant Near the Cutting Edge in High Speed Machining of Difficult-to-Cut Materials

This study investigates film boiling of coolant as a cooling inhibitor in a narrow wedge-shaped space between the tool flank face and the machined surface of a workpiece, observed during high-speed turning of stainless steel SUS304 and nickel-based superalloy Inconel 718. The boiling, likely triggered by high surface temperatures at both the face and surface close to the cutting edge, impedes coolant access to the tool tip area and efficient cooling. Therefore, the impact of coolant pressure on the boiling zone size was initially explored across pressures ranging from 0.1 to 20 MPa. A burn mark band due to coolant boiling, distinctly visible on the flank face of an insert with a yellow hard coating, expanded over cutting time. The film boiling area width, or the distance from the flank wear area to the band, decreased with increasing coolant pressure, reflecting the enhanced cooling ability and tool life with high-pressure coolant. Applying Boyle–Charles’ law to film boiling indicated that vapor pressure was directly related to coolant velocity rather than pressure. In contrast, the boiling area width increased with increasing cutting speed.

Laparoscopic Colectomy for Cecal Cancer and Intestinal Malrotation: A Case Report.

Intestinal malrotation (IM) often remains undetected until adulthood, being discovered during testing or surgery for other comorbidities. Preoperative understanding of this anatomical abnormality is crucial. An 80-year-old woman presented with cecal cancer. Three-dimensional computed tomography (CT) revealed that the cecum was located at the midline of the abdominal cavity, the duodenum did not cross the midline, and the ileocolic vein ran to the left. Clinically diagnosed with stage IVc cecal cancer complicated by IM, the patient underwent laparoscopic surgery. The ascending colon and cecum were not fixed to the retroperitoneum. The duodenum lacked the second, third, and fourth portions and the small bowel was distributed on the left and right sides of the abdominal cavity. Adhesions had shortened the mesentery, which were released close to their normal positions. Although laparoscopic surgery is superior to open surgery in terms of securing the field of view in a narrow space, providing a magnifying effect, and minimal invasiveness, it has a limited field of view and is inferior in terms of grasping the overall anatomy, which may be disadvantageous in cases of anatomical abnormalities. Colorectal cancer with IM is rare; however, the rate of preoperative diagnosis seems to be increasing thanks to improvements in diagnostic imaging, such as three-dimensional CT scans. In this study, we also reviewed 49 cases of colorectal cancer associated with IM.

Seeding Atomic Silver into Internal Lattice Sites of Transition Metal Oxide for Advanced Electrocatalysis.

Transition metal oxides (TMOs) are widely studied for loading of various catalysts due to their low cost and high structure flexibility. However, the prevailing close-packed nature of most TMOs crystals has restricted the available loading sites to surface only, while their internal bulk lattice remains unactuated due to the inaccessible narrow space that blocks out most key reactants and/or particulate catalysts. Herein, using tunnel-structured MnO2, this study demonstrates how TMO's internal lattice space can be activated as extra loading sites for atomic Ag in addition to the conventional surface-only loading, via which a dual-form Ag catalyst within MnO2 skeleton is established. In this design, not only faceted Ag nanoparticles are confined onto MnO2 surface by coherent lattice-sharing, Ag atomic strings are also seeded deep into the sub-nanoscale MnO2 tunnel lattice, enriching the catalytically active sites. Tested for electrochemical CO2 reduction reaction (eCO2RR), such dual-form catalyst exhibits a high Faradaic efficiency (94%), yield (67.3mol g-1 h-1) and durability (≈48h) for CO production, exceeding commercial Ag nanoparticles and most Ag-based electrocatalysts. Theoretical calculations further reveal the concurrent effect of such dual-form catalyst featuring facet-dependent eCO2RR for Ag nanoparticles and lattice-confined eCO2RR for Ag atomic strings, inspiring the future design of catalyst-substrate configuration.

Alternative Agronomic Management Practices Through Fertilizer, Irrigation, and Plant Density Adjustments for Hybrid Fodder Sorghum (<em>Sorghum bicolor</em> L. Moench)

Purpose: The basal fertilizer recommendation for growing hybrid fodder sorghum varieties in uplands involves relatively high quantities of Urea, TSP, and MOP, with rates of 100:90:65 kg/ha for SX-17 and 150:62:37 kg/ha for Dairy green. For both initial and ratoon crops, it is recommended to apply 150 kg/ha of urea as a top dressing. This study investigated the impact of reducing the fertilizer application rate to 75% and employing alternative agronomic practices on hybrid fodder sorghum varieties grown in lowland fallow paddy fields in the dry zone during the Yala and third seasons.Research Method: The agronomic management practices, including four basal fertilizer rates (Urea:TSP: MOP) of 100:90:65 (BD1), 150:62:37 (BD2), 75:68:49 (BD3), and 113:47:28 (BD4); two top-dressing urea fertilizer rates of 150 (TD1) and 112.5 kg/ha (TD2); two plant spacing configurations of 45x30 (WS) and 45x15 cm (NS); and two irrigation interval regimes, 5 days up to 30 days followed by 8 days (SI) and 7 days up to 30 days followed by 10 days (LI) were investigated. These factors were combined into four agronomic management packages: AMP1 (BD1, TD1, WS, SI), AMP2 (BD2, TD1, NS, SI), AMP3 (BD3, TD2, WS, LI), and AMP4 (BD4, TD2, NS, LI). The study used a split-plot design with three replicates and assessed various parameters, including plant height, stem diameter, plant weight, leaf area index (LAI), number of tillers, and fodder yield in both the initial fodder sorghum crop and the first ratoon crop. The crops were harvested when 50% of the panicles on the plants were at the milk and dough grain stages.Findings: The initial crop exhibited significantly heavier plants having wider stems when grown with higher fertilizer rates, wider plant spacing, and shorter irrigation intervals in AMP1. Irrespective of fertilizer rate and irrigation interval, the initial crops tended to have higher LAI with narrow plant spacing in AMP2 and AMP4. In the ratoon crops, wider plant spacing in AMP1 and AMP3 resulted in clumps with a greater number of tillers and heavier plants. However, the ratoon crops also exhibited higher LAI with narrow plant spacing in AMP2 and AMP4. Total fodder dry matter yield (DMY) in AMP2 and AMP4 with narrow plant spacing was significantly greater for both varieties. Furthermore, the DMY was not affected by fertilizer rate and irrigation interval.Value: Hybrid fodder sorghum varieties (SX-17 and Dairygreen) cultivated at higher plant density, along with reduced fertilizer (75%) and irrigation, can produce comparable fodder yield (21.1 and 26.1 MT/ha, respectively) to those grown at lower plant density with more fertilizer and irrigation (24.6 and 28.1 MT/ha, respectively) in lowland paddy fields during Yala and third seasons in the dry zone.

Probabilistic model for predicting fire casualties in an urban traffic tunnel

Fire accidents could result in severe casualties in an urban traffic tunnel due to the concentration of users and narrow space. However, fire casualties could not be easily predicted due to the complicated evacuation environment and uncertainty in personnel distribution. This paper proposes a probabilistic model to predict the fire casualties in an urban traffic tunnel with only natural ventilation, considering the effects of the thermal and carbon monoxide distribution, in addition to the arrangement of various vehicles. First, the general framework of the probabilistic model was put forward and the methods to analyze the fire scenario, evacuation process, casualty criteria, and probability curve were introduced in details. Then, a case study based on a three-lane traffic tunnel was conducted, and the effects of maximum heat release rate on probabilistic curve of fire casualties were discussed. The results showed that the fire casualty increased with the maximum heat release rate. Besides this, when the distance between fire source and evacuation exit was reduced to 150 m, the impact of maximum heat release rate would decrease significantly. This study made notable contributions toward evaluating the personnel loss in tunnel fire and laid a foundation for assessing and improving the resilience of tunnel.

Enhanced sodium-storage performances of crumpled MXene nanosheets via alkali treatment–induced active ammonium ions

Ti3C2Tx MXene demonstrates excellent potential as an anode material for sodium-ion capacitors. However, the narrow interlayer spacing and self-stacking phenomenon limit its applicability. In this study, we demonstrate an easy two-step method involving freezing and crumpling of MXene nanosheets to improve their Na-ion storage via the addition of ammonium ions (referred to as FCM nanosheets). Flat MXene particles aggregate and undergo folding in an alkaline solution. Ammonium ions can penetrate the gaps between MXene nanosheets, expanding interlayer spaces and inducing the formation of folds. Compared to MXene nanosheets, FCM nanosheets exhibit improved ion transfer kinetics and additional high capacity owing to the intercalated ammonium ions. The manufactured FCM anode exhibits remarkable electrochemical properties, including a high specific capacity of 313 mAhg−1 and stability over 15,000 cycles.

Arthroscopy strategy to obtain surgical access to peripheral lateral tibial plateau for arthroscopic assisted reduction & internal fixation of fractures

Current standard of open surgical approach for lateral tibial plateau fractures is lateral submeniscal arthrotomy for obtaining visual and instrumentation access to peripheral meniscus covered part where fractures are often located. Anterior parapatellar arthrotomy is used less often as it is associated with soft tissue complications and provides access to only central uncovered part. Lateral submeniscal arthrotomy achieves necessary access by surgical division of meniscotibial attachments and superior retraction of thus detached meniscus. Arthroscopic reduction and internal fixation (ARIF), with its portals placed anteriorly obtains efficient viewing as well as instrumentation access to central part but; only tangential viewing access to peripheral concealed part of joint surface in mid-lateral and posterior part and none at all in anterior and anterolateral part. Further, considering meniscotibial attachment of meniscus remains intact in ARIF, manoeuvrability of instruments in narrow submeniscal space even in posterior and mid-lateral area is severely constrained. ARIF as it is practiced now, therefore remains merely a monitoring and evaluation tool at least in peripheral meniscus concealed area. Direct elevation of depressed articular fragment often undertaken in ORIF by submeniscal arthrotomy, remains therefore unexploited in ARIF. This article describes a strategy to achieve arthroscopically; visual and instrumentation access similar to open submeniscal arthrotomy to conduct interventions equivalent to those carried out in ORIF.

Overview of in-bore pipe cutting and welding tools for the maintenance of CFETR and EU-DEMO

In the remote maintenance of fusion nuclear devices, one of the most challenging tasks is the cutting and the re-welding of large pipes that feed the breeding blanket. The narrow space around some of the pipes hinders the suitability of conventional orbital processes. For such pipes, both the cutting system and the welding one have to be compact enough to move along their interior, lock against the pipe inner wall and keep the position during the operation. The inner diameter and thickness of the pipes together with the other severe requirements related to the remote maintenance drive the choice of the processing technologies and the design of the tooling configuration. In this work, activities carried out by a joint team from EU-DEMO and CFETR on the in-bore cutting and welding operations are presented and discussed. For the in-bore cutting system a solution for the simultaneous parting and bevelling has been developed opting for a mechanical cutting with a pair of symmetrically distributed knives. A preliminary dimensioning of the motors has been made assuming the radial feed and the cutting speed of the knives. For the in-bore welding system, the TIG welding with filler wire has been considered as the most reliable and suitable technology within prescribed requirements and constraints. A multi-pass welding operation has been assumed. A conceptual design of both tools has been developed. The space constraint remains the most critical issue. Further verification and validation work is planned in close collaboration between European laboratories and the Comprehensive Research fAcility for Fusion Technology (CRAFT) at ASIPP in Hefei.

Surgical training in extraperitoneal laparoscopic para‑aortic lymphadenectomy for the treatment of gynecological cancer using a Thiel‑embalmed cadaver.

The extraperitoneal laparoscopic approach (ELPAN) for para-aortic lymphadenectomy provides excellent visibility of the left side of the aorta, thus facilitating surgery in the retroperitoneal space. This technique is highly complex compared with the transperitoneal approach. In particular, advanced techniques are required to develop an appropriate surgical field in the narrow retroperitoneal space; therefore, surgeons need to undergo a significant amount of training to become competent. A variety of tools are available for surgical training but are limited by their ability to reproduce complex anatomy. Thus, cadavers may represent the most suitable tool for learning this unique technique. The present study describes a surgical training protocol for the ELPAN technique using a Thiel-embalmed human cadaver and provides a step-by-step description of the ELPAN technique performed at Okayama University (Okayama, Japan). A 72-year-old Thiel-embalmed female cadaver was used to develop a protocol for surgical training in the ELPAN technique that effectively reproduced the methodology required in clinical practice. A training method for ELPAN surgery was developed and successfully completed using the Thiel-embalmed cadaver that secured the surgical field in the retroperitoneal space and permitted resection of the lymph nodes. The Thiel-embalmed cadaver tissue possessed excellent properties for surgical training, including color tone, flexibility, and the membrane structure of connective and fat tissues. In addition, this method of fixation preserved stiffness and elasticity of the peritoneum, although large vessels were slightly fragile and poorly extensible. Surgical training using a Thiel-embalmed human cadaver represents a valuable option for learning the ELPAN surgical technique. However, this technique may be unsuitable for training in perivenous manipulation. To the best of our knowledge, this is the first report to describe the use of Thiel-embalmed cadavers as a tool for surgeons to undergo training in the ELPAN technique.

Simulation of range code tracking loop for multipath mitigation in NavIC receiver

Abstract The Operational Navigation with Indian Constellation (NavIC) comprises seven satellites in the orbit, including three in geostationary orbit (GEO) and four in geosynchronous orbit (GSO). NavIC provides both Standard Positioning Service and Restricted Service, using L5 (1176.45 MHz) and S1 (2492.028 MHz) frequencies, with coverage extending 1500 km around the mainland of India. In an urban canyon, multipath interference severely reduces the precision and reliability of NavIC positioning. Many current multipath mitigation techniques often exhibit high computational requirements or reliance on external assistance. In this paper, a ranging code tracking loop is proposed that can sustain either a late or early branch in contrast to the Narrow-Spacing (NS) correlation technique for mitigating multipath for NavIC receiver. The design of proposed code tracking loop is based on steepest descent algorithm. The findings demonstrate that, in terms of calculation time and code multipath mitigation, the suggested technique performs better than both Multipath Estimated Delay Locked Loop (MEDL) and NS correlation. The proposed method produces less than 0.016 chips for the tracking error Standard Deviation (STD). In addition, the recommended method takes 24 % less computation time.

Remote cross-resonance gate between superconducting fixed-frequency qubits

High-fidelity quantum state transfer and remote entanglement between superconducting fixed-frequency qubits have not yet been realized. In this study, we propose an alternative remote cross-resonance gate. Considering multiple modes of a superconducting coaxial cable connecting qubits, we must find conditions under which the cross-resonance gate operates with a certain accuracy even in the presence of qubit frequency shifts due to manufacturing errors. For 0.25- and 0.5 m cables, remote cross-resonance gates with a concurrence of >99.9% in entanglement generation are obtained even with ±10 MHz frequency shifts. For a 1 m cable with a narrow mode spacing, a concurrence of 99.5% is achieved by reducing the coupling between the qubits and cable. The optimized echoed raised-cosine pulse duration is 150–400 ns, which is similar to the operation time of cross-resonance gates between neighboring qubits on a chip. The dissipation through the cable modes does not considerably affect the obtained results. Such high-precision quantum interconnects pave the way not only for scaling up quantum computer systems but also for nonlocal connections on a chip.

Designing User Interface Of Travel Mobile Application Based On Artificial Intelligence

Indonesia is a country with many potential places that can be used as tourist destinations. The Central Statistics Agency (BPS) states that as of 2021 Indonesia has a total of 2,552 destinations in the tourism industry. The development of the tourism industry in Indonesia was hampered by the pandemic that had hit the country for approximately 2 years, which caused a narrow space for tourists to move. On the other hand, the use of digital technology has increased during the pandemic era. Artificial intelligence is one of the technologies that also developed during the pandemic. This technology allows for a program to think like a human and help facilitate daily activities and work. This design has the aim of creating an application display design that can implement artificial intelligence in a mobile application for the needs of the tourism industry. The method used in this design is the Design Thinking method popularized by Kelley and Brown from IDEO, consisting of the Emphatize, Define, Ideate, Prototype, and Testing stages. The result of this design is a design of a mobile application interface for the tourism industry, with the implementation of artificial intelligence and various features, and contains information about various tourist destinations, as well as designing its trials easily by users.

Turbulent flow around submerged foundation arrays for ocean energy

Submerged ocean energy foundations experience dynamic loading due to the non-uniform approach velocity and wake propagation behind them. This paper investigates the unsteady turbulent flow dynamics and propagation of large-scale vortical structures around submerged foundations using a large eddy simulation. The computational domain constituted an array of eight foundations arranged in an inline configuration of two columns. Two streamwise spacing, x = 6D and 8D, are investigated at an approach Reynolds number, based on the channel hydraulic diameter, of 8.5 × 104. The parametric study reveals the expected changes in wake recovery and their impact on the downstream foundations. The narrow spacing promotes intense wake interactions, while larger inline spacing causes the entrainment of ambient flow, thereby limiting the wake interaction with the downstream foundations. A two-point correlation analysis reveals larger integral turbulent timescales for the 6D spacing compared to the 8D spacing. The separated flow around the foundations is characterized by eddies shed at varying frequencies. Multiple spectra peaks dominate at low frequencies, signifying dominant energy-containing large-scale vortices coupled with periodic excitation of the vortices as energy is transferred from the large-to small-scale turbulent structures.

Design of a Quadruped Robot with Morphological Adaptation through Reconfigurable Sprawling Structure and Method

Morphological adaptation is crucial for animals and robots in navigating unstructured environments. In this article, a quadruped robot with a reconfigurable sprawl posture and posture transformation strategy is proposed, which can transform between different sprawl postures to cope with complex environments and adapt to a dorsal downward fall posture through reverse sprawling. First, the function and structure of the robot are described, including an analysis of the Hawken mechanism's endpoint trajectories and a regional examination of the robot leg's fundamental components, establishing a relationship between the trajectory characteristics and linkage length. Second, the robot posture transformation strategy is analysed, obtaining the geometric dimensional constraints and feasibility regions of the posture transformation. The posture transformation process is quantified, and the robot transformation gait and drive functions are designed. The robot gait and velocity regulation are implemented based on a neural control architecture. Finally, the robot's locomotion and posture transformation are tested using four high‐speed cameras. The results show that the robot can crawl on an inclined surface and continue crawling in a dorsal downward posture post‐fall. Additionally, the robot effectively navigates obstacles and narrow spaces after posture transformation.

Noise reduction of electron holography observations for a thin-foiled Nd-Fe-B specimen using the wavelet hidden Markov model

In this study, we investigate the effectiveness of noise reduction in electron holography, based on the wavelet hidden Markov model (WHMM), which allows the reasonable separation of weak signals from noise. Electron holography observations from a Nd2Fe14B thin foil showed that the noise reduction method suppressed artificial phase discontinuities generated by phase retrieval. From the peak signal-to-noise ratio, it was seen that the impact of denoising was significant for observations with a narrow spacing of interference fringes, which is a key parameter for the spatial resolution of electron holography. These results provide essential information for improving the precision of electron holography studies.

Toward Modeling the Structure of Electrolytes at Charged Mineral Interfaces Using Classical Density Functional Theory.

The organization of water molecules and ions between charged mineral surfaces determines the stability of colloidal suspensions and the strength of phase-separated particulate gels. In this article, we assemble a density functional that measures the free energy due to the interaction of water molecules and ions in electric double layers. The model accounts for the finite size of the particles using fundamental measure theory, hydrogen-bonding between water molecules using Wertheim's statistical association theory, long-range dispersion interactions using Barker and Henderson's high-temperature expansion, electrostatic correlations using a functionalized mean-spherical approximation, and Coulomb forces through the Poisson equation. These contributions are shown to produce highly correlated structures, aptly rendering the layering of counterions and co-ions at highly charged surfaces and permitting the solvation of ions and surfaces to be measured by a combination of short-range associations and long-ranged attractions. The model is tested in a planar geometry near soft, charged surfaces to reproduce the structure of water near graphene and mica. For mica surfaces, explicitly representing the density of the outer oxygen layer of the exposed silica tetrahedra allows water molecules to hydrogen-bond to the solid. When electrostatic interactions are included, water molecules assume a hybrid character, being accounted for implicitly in the dielectric constant but explicitly otherwise. The disjoining pressure between approaching like-charged surfaces is calculated, demonstrating the model's ability to probe pressure oscillations that arise during the expulsion of ions and water layers from the interfacial gap and predict strong interattractive stresses that form at narrow interfacial spacing when the surface charge is overscreened. This interattractive stress arises not due to in-plane correlations under strong electrostatic coupling but due to the out-of-plane structuring of associating ions and water molecules.

Pterostilbene Protects against Osteoarthritis through NLRP3 Inflammasome Inactivation and Improves Gut Microbiota as Evidenced by In Vivo and In Vitro Studies.

Osteoarthritis (OA) is a persistent inflammatory disease, and long-term clinical treatment often leads to side effects. In this study, we evaluated pterostilbene (PT), a natural anti-inflammatory substance, for its protective effects and safety during prolonged use on OA. Results showed that PT alleviated the loss of chondrocytes and widened the narrow joint space in an octacalcium phosphate (OCP)-induced OA mouse model (n = 3). In vitro experiments demonstrate that PT reduced NLRP3 inflammation activation (relative protein expression: C: 1 ± 0.09, lipopolysaccharide (LPS): 1.14 ± 0.07, PT: 0.91 ± 0.07, LPS + PT: 0.68 ± 0.04) and the release of inflammatory cytokines through NF-κB signaling inactivation (relative protein expression: C: 1 ± 0.03, LPS: 3.49 ± 0.02, PT: 0.66 ± 0.08, LPS + PT: 2.78 ± 0.05), ultimately preventing cartilage catabolism. Interestingly, PT also altered gut microbiota by reducing inflammation-associated flora and increasing the abundance of healthy bacteria in OA groups. Collectively, these results suggest that the PT can be considered as a protective strategy for OA.