Narrow Endemic Plant Species Research Articles

A multifaceted field sampling approach for the management of extremely narrow endemic vascular plant species.

Extremely narrow endemic plant species (ENEs) are generally connected with microrefugia characterized by particular environmental conditions. In-depth knowledge of the ecological requirements of ENEs is fundamental to plan appropriate conservation measures. Using cross-cutting technology, this paper gives a multifaceted approach to collect on-site data on the ecology of ENEs, defines the protocols for a correct sampling design and describes the type of equipment, the time and expenditure needed. Our sampling approach is based on two orthogonal transects, long enough to extrapolate the whole ecological gradient across the area of occupancy of the target species. Microclimatic data are recorded all along the transects through iButton technology, plus a weather station installed at the intersection of the transects. Microtopographic data are recorded with high-resolution digital elevation model and sub-metric GPS. Edaphic data are recorded along the transects through standard soil analyses and on-site evaluation of the seasonal decomposition rate of organic matter. Additionally, vegetation sampling in 4m2 plots and on-site germination tests allow to collect data on auto- and synecological factors that regulate the life cycle of the target species. Our approach has proved to be cost-effective and efficient in terms of time spent in the field against the data collected. The most demanding activities were the establishment of the transects and the vegetation sampling. The time spent downloading microclimatic data and testing seed germination was relatively short. Our sampling design allows: (i) to catch as much micro-topographic variability as possible, both within and out of the tolerance range of the target species, (ii) to minimize the risk of recording identical micro-topographic conditions compared with a random sampling scheme, and (iii) to ensure quick and relatively easy retrieval of the plots and the equipment both on a multi-seasonal and multi-annual basis.

Sentinel 2 images enable reliable prediction of fine‐scale habitat dynamics of narrow endemic plant species in serpentine soils

AbstractAimsSerpentine soils are rare globally, covering <1% of the land surface, but frequently harbouring large numbers of narrow endemic species. Many serpentine areas are endangered and would benefit from actions informed by species distribution models (SDMs). Here, we assessed, for the first time, the potential use of remotely sensed descriptors of water and carbon cycles derived from Copernicus Sentinel 2 images for fine‐scale habitat characterization and mapping of narrow endemic serpentine‐tolerant species.LocationWe focused on three endemic species with a very limited distribution in the Serra de Careón ultramafic outcrop, a regional plant biodiversity hotspot in Spain.MethodsWe developed SDMs based solely on remotely sensed descriptors of plant water content and habitat dynamics estimated from multitemporal Sentinel 2 images.ResultsThe predictive capacity of the models was very high (area under the receiver operating characteristic curve (AUC) > 0.9, sensitivity > 90 and specificity > 84), even for projections beyond the range of data used for calibration (AUC > 0.8, sensitivity > 77 and specificity > 81). Overall, the suitable habitat areas predicted by the models were similar for all three species (Schoener’s D metric > 0.7). The annual mean and coefficient of variation of the Normalized Difference of Water Index provided a good representation of the hydromorphic soils with serpentine clay minerals and characterized by the formation of temporary pools during winter months. The annual mean and coefficient of variation of the Modified Soil Adjusted Vegetation Index were also important predictors owing to their capacity to capture the serpentine habitat conditions, characterized by open vegetation and highly exposed soil.ConclusionsThese findings confirm the potential of ecosystem functioning descriptors derived from spectral Sentinel 2 indices as a cost‐effective means of characterizing the ecological niche of endemic plant species in serpentine habitats and as a promising tool to support their conservation and monitoring.

Genetic and ecological consequences of recent habitat fragmentation in a narrow endemic plant species within an urban context

Understanding the timescales that shape spatial genetic structure is pivotal to ascertain the impact of habitat fragmentation on the genetic diversity and reproductive viability of long-lived plant populations. Combining genetic and ecological information with current and past fragmentation conditions allows the identification of the main drivers important in shaping population structure and declines in reproduction, which is crucial for informing conservation strategies. Using historic aerial photographs, we defined the past fragmentation conditions for the shrub Conospermum undulatum, a species now completely embedded in an urban area. We explored the impact of current and past conditions on its genetic layout and assessed the effects of genetic and environmental factors on its reproduction. The historically high structural connectivity was evident in the genetics of the species. Despite the current intense fragmentation, we found similar levels of genetic diversity across populations and a weak spatial genetic structure. Historical connectivity was negatively associated with genetic differentiation among populations and positively related to within-population genetic diversity. Variation partitioning of reproductive performance explained ~ 66% of the variance, showing significant influences for genetic (9%), environmental (15%), and combined (42%) fractions. Our study highlights the importance of considering the historical habitat dynamics when investigating fragmentation consequences in long-lived plants. A detailed characterization of fragmentation from 1953 has shown how low levels of genetic fixation are due to extensive gene flow through the non-fragmented landscape. Moreover, knowledge of the relationships between genetic and environmental variation and reproduction can help to implement effective conservation strategies, particularly in highly dynamic landscapes.

Range extension and conservation status of the rare Solanaceae shrub, Solanum conocarpum.

BackgroundThe British Virgin Islands and the US Virgin Islands, two island groups located in the Caribbean archipelago, hold unique plant diversity and high endemism. Until recently, Solanumconocarpum was considered a rare plant species endemic to the island of St. John in the US Virgin Islands. Ongoing botanical surveys in this region are revealing new populations and refining our understanding of the distribution of these narrow endemic plant species. The objective of this paper is to assess the conservation status of S.conocarpum, including a review of its geographic range, population numbers, threats and conservation actions needed for its long-term survival.New informationIn this paper, we present new occurrences for S.conocarpum, extending its geographic range to a new island, Tortola and new territory, the British Virgin Islands. Despite this range expansion, this species is evaluated as Endangered (EN), based on Criteria B1b(iii,v)+2b(iii,v)+C2a(i), according to the IUCN Red List Categories and Criteria. The extent of occurrence (EOO = 46 km2) and area of occupancy (AOO = 20 km2) are highly restricted. On St. John (US Virgin Islands), the historically recorded individuals at Reef Bay, Europa Ridge and Sabbat Point are now considered extirpated due to disturbance from development compounded by invasive species, as well as the impact of feral ungulates and drought stress. These threats are impacting the species across the whole island of St. John and contributing to a continuing decline of suitable habitat, despite the island being a National Park. On the island of Tortola, the species occurs on unprotected lands subject to development and habitat modification and decline by feral ungulates. Based on these threats acting separately across the two islands, two locations were defined. The estimated total number of mature individuals ranges between 150 and 250, with the largest subpopulation at Nanny Point in the US Virgin Islands, containing 108 mature individuals. Conservation action, focused on protecting this species' habitat, is urgently needed.

Fine-scale species distribution modelling and genotyping by sequencing to examine hybridisation between two narrow endemic plant species

Hybridization has an important and often positive role in plant evolution. However, it can also have negative consequences for species. Two closely related species of Ornduffia are endemic to the Porongurup Range in the South West Australian Global Biodiversity Hotspot. The rare Ornduffia calthifolia is found exclusively on the summits, while O. marchantii is more widely dispersed across a greater range of elevation and is not considered threatened. Hybridisation in suitable overlapping habitat has been suspected between them for decades. Here we combine genotyping by sequencing to verify hybridisation genetically, and fine scale (2 m resolution) species distribution modelling (SDM) to test if hybrids occur in suitable intersecting habitat. From a study area of c. 4700 ha, SDM identified c. 275 ha and c. 322 ha of suitable habitat for O. calthifolia and O. marchantii, respectively. We identified range overlap between species of c. 59 ha), which enveloped 32 individuals confirmed to be hybrids. While the hybrids were at the margin of suitable habitat for O. marchantii, their preference for elevated habitat was closer to the more narrowly distributed O. calthifolia. The combination of genetic data and fine scale spatial modelling approaches enabled a better understanding of hybridisation among taxa of conservation significance. However, the level to which hybrid proliferation and competition for habitat presents as a threat to O. calthifolia is currently unknown and requires priority in conservation management given the threats from global warming and disturbance by tourism.

Breeding system and inbreeding depression in a translocated population of the endangered plant Dianthus morisianus (Caryophyllaceae)

Know the breeding system of endemic plants is important to design conservation strategies. Translocations are actions to improve the survival prospects of the species, but nowadays there are only a few studies that analyse their success and make a comparison between translocation and the natural populations. Dianthus morisianus is a threatened narrow endemic plant species growing on sand dunes in SW Sardinia (Italy). The objective of this study was to assess the breeding system, the presence of inbreeding depression and pollen limitation, as well as the success of the plant translocation. All these results were compared with those from the single natural population. The breeding system was tested through five pollination treatments and the reproductive success was analysed by the fruit set, seed set, seed weight, germination and mortality rate. The translocated population behaved like the natural one on fruit and seed formation. Autonomous self-pollination was lower than the other treatments regarding fruit set and seed/ovule ratio in the two studied populations. The species is self-compatible and presents partial self-fertility. The selfing rate was higher in the translocated population and the inbreeding depression presented low values for the natural population, while the translocated population presented negative values. Neither of the populations suffered pollen limitation. The species did not present reproductive problems and it is pollinator dependent. Moreover, the translocated population demonstrated high success after five years, as an increase of the population area and new recruited plants was observed; the offspring were able to flower, fruiting and reproduce. This translocation success increases the survival prospects of the species.

Predicting population viability of the narrow endemic Mediterranean plant Centaurea corymbosa under climate change

Climate change is a growing threat for global biodiversity, in particular for narrow endemic species. The Mediterranean region, which harbors an exceptional biodiversity, has been identified as one of the most sensitive regions to climate change. Based on a 22-year monitoring period, we analyzed the dynamic and viability of the six extant populations of a narrow endemic plant species of the Mediterranean area, Centaurea corymbosa, to predict their fate under two climatic scenarios. We constructed matrix projection models to calculate current asymptotic growth rates and to perform stochastic projections including both demographic and environmental stochasticity. Neither asymptotic growth rates nor their temporal variance were linked to population size and age at flowering. Randomization tests showed that asymptotic growth rates were significantly different among years but not among populations. An increase in temperature and a decrease in the number of wet days had a negative impact on the whole life-cycle, particularly in the summer period, and thus reduced asymptotic growth rates. Stochastic projections showed that an increased frequency of extreme climatic events increased population extinction risk and decreased mean time to extinction. The warm scenario had a more dramatic impact on population viability than the dry scenario. Management recommendations are proposed to increase population viability of endangered plant species such as C. corymbosa that face climate change.

Ten nuclear microsatellites markers cross-amplifying in Scaevola montana and S. coccinea (Goodeniaceae), a locally common and a narrow endemic plant species of ultramafic scrublands in New Caledonia

We developed ten microsatellites markers to study the population genetic structure of two ultramafic scrublands species in New Caledonia, Scaevola montana a common species used in ecological restoration of mining sites, and S. coccinea a narrow endemic species of the Tontouta valley. The markers were isolated from S. montana. Nine loci are polymorphic in S. montana; eight were polymorphic in S. coccinea. The narrow endemic species showed lower variation at these loci than S. montana. There is a significant heterozygosity deficit in S. montana at four loci, and a global significant inbreeding coefficient across all loci (F IS = 0.159). In contrast, the tested populations of S. coccinea did not show significant deviations from Hardy–Weinberg expectations. These markers are now being used in conservation genetic studies aiming to understand the biology of these species and to provide information to guide restoration programmes.

Invasive competitor and native seed predators contribute to rarity of the narrow endemicAstragalus sinuatusPiper

The conservation of rare plant species hinges on our ability to identify the underlying mechanisms that limit rare plant populations. Theory on rarity suggests that both predispersal seed predation and competition can be important mechanisms influencing abundance and/or distribution of rare plant populations. Yet few studies have tested these interactions, and those that have evaluated each mechanism independently. Astragalus sinuatus Piper (Whited's milkvetch) is a narrow endemic plant species restricted to eight populations within a 10-km2 area in eastern Washington. We used experimental and observational methods to test the effects of native insect predispersal seed predators and an invasive grass (Bromus tectorum L. [cheatgrass]) on seed set and population density of A. sinuatus. We quantified per capita seed production and pod predation rates across four sites and among four years. Seed predation rates were high across four sites (66-82%) and all years (65-82%). Experimental reduction of predispersal seed predators significantly increased per capita seed set of A. sinuatus (164-345%) at two experimental sites. Concurrently, two seed addition experiments demonstrated the effect of seed loss and presence of B. tectorum on seedling recruitment and establishment of A. sinuatus over four growing seasons. In the first seed addition experiment, we found no difference in recruitment and establishment between low (40) and high (120) seed addition levels. In the second addition experiment (one level of addition; 40 seeds), we found that recruitment and survivorship increased 200% in plots where B. tectorum was removed compared to plots where B. tectorum was present. Thus, seed addition had no impact in the presence of B. tectorum; conversely, in the absence of B. tectorum, seed addition was highly effective at increasing population numbers. Results suggest that, in areas where B. tectorum is present, recruitment is site limited, and it is seed limited when B. tectorum is absent. We recommend that managers reduce B. tectorum in an effort to increase population growth of A. sinuatus; in areas where B. tectorum is absent, short-term reduction of insect predators should be considered as a strategy to increase population growth of this rare species.

Distribution, status and conservation of a Critically Endangered, extremely narrow endemic: Lamyropsis microcephala (Asteraceae) in Sardinia

AbstractThe aims of this work were to verify the distribution and population size of Lamyropsis microcephala (Asteraceae), characterize its habitat, assess its conservation status and initiate conservation measures for this Critically Endangered species. Distribution was determined by field surveys and mapping. To estimate population size and density 81 permanent monitoring plots were randomly established. Ex situ conservation measures were activated by harvesting and appropriate storage of seed. We confirmed the presence of L. microcephala at two previously known sites and found it in two previously unknown localities. The areas in which the species occur vary from 200 to 240,000 m2, at altitudes of 1,450–1,820 m, on slopes of 15–45° with aspects from north to west. The estimated number of ramets per population varied from c. 2,000 to c. 2,000,000, with a mean density of 8.29 ± SD 14.3 to 10.33 ± SD 10.05 ramets m-2. Our findings confirmed the Critically Endangered status of this species, although with different criteria. This study is an example of an integrated approach for the conservation of an extremely narrow endemic plant species and may be useful for other little-known threatened species for which urgent conservation measures are needed.

Conservation assessment of Aquilegia paui (Ranunculaceae): a case study of an extremely narrow endemic

AbstractThe extremely rare Aquilegia paui (Ranunculaceae) was described in 1920 but was not found again until 1999, when it was discovered in the Parc Natural dels Ports in Tarragona Province, Spain. This species had been confused with the widespread A. vulgaris and consequently its taxonomic and conservation status had been misinterpreted. Based on the limited range of A. paui and the level of disturbance of its habitat we recommend that it is categorized as Endangered on the IUCN Red List. We summarize the conservation actions available for such extremely narrow endemic plant species and make appropriate recommendations for the conservation of A. paui.

Germination requirements of the alpine endemic Silene elisabethae Jan: effects of cold stratification, light and GA3

Summary Silene elisabethae Jan (Caryophyllaceae) is a narrow endemic plant species that occurs in calcareous grasslands of the Southern Italian Alps, and is protected by National and Regional Red Lists (IUCN). Seed germination requirements of this species were studied for the fi rst time with the aim of producing appropriate germination protocols for use in ex situ conservation. Seeds were collected from three populations and sown on agar in the laboratory. Increasing durations of cold stratifi cation resulted in an increase in germination from 20 to 90%. In addition, GA3 could substitute for a cold stratifi cation requirement resulting in 90 to 100% germination in the absence of chilling. All populations germinated to higher levels in the light than dark, and populations from the coldest collection site germinated better at low temperatures, suggesting adaptation of seed germination behaviour to the local microclimate. Our results indicate that seeds of S. elisabethae exhibit non-deep physiological dormancy and that light plays an important role as a germination cue.

Potential vulnerability of Namaqualand plant diversity to anthropogenic climate change

We provide a position paper, using a brief literature review and some new modelling results for a subset of succulent plant species, which explores why Namaqualand plant diversity might be particularly vulnerable to anthropogenic climate change despite presumed species resilience under arid conditions, and therefore a globally important test-bed for adaptive conservation strategies. The Pleistocene climate-related evolutionary history of this region in particular may predispose Namaqualand (and Succulent Karoo) plant endemics to projected climate change impacts. Key Succulent Karoo plant lineages originated during cool Pleistocene times, and projected air temperatures under anthropogenic climate change are likely to exceed these significantly. Projected rainfall patterns are less certain, and projections of the future prevalence of coastal fog are lacking, but if either of these water inputs is reduced in concert with rising temperatures, this seems certain to threaten the persistence of, at least, narrow-endemic plant species. Simple modelling approaches show strong reduction in spatial extent of bioclimates typical of Namaqualand within the next five decades and that both generalist species with large geographic ranges, and narrow-range endemics may be susceptible to climate change induced loss of potential range. Persistence of endemics in micro-habitats that are buffered from extreme climate conditions cannot be discounted, though no attempts have been made to address this shortcoming of broader scale bioclimatic modelling. The few experimental data available on elevated temperature and drought tolerance suggest susceptibility of leaf succulent species, but high drought tolerance of non-succulent shrubs. Both species-level monitoring and further experimental work is essential to test and refine projections of climate change impacts on species persistence, and the implications for conservation.

Can the preservation of historical relicts permit the conservation of endangered plant species? The case of Silene sennenii (Caryophyllaceae)

Allozyme electrophoresis was used to measure levels and distribution of genetic diversity in the critically endangered (CR) Silene sennenii (Caryophyllaceae), a narrow endemic plant species found in northeastern Catalonia (Spain). At present, only 5 populations remain, containing no more than 5,000 individuals, which are subjected to several human pressures such as habitat fragmentation and land use changes. Interestingly, the largest population is located in and around a fortress built in the XVIII century, a circumstance which might facilitated its preservation until now. From the 21 satisfactorily interpreted loci, low levels of genetic variation were detected (P = 20.9, A = 1.31 and He = 0.071), which may be related to small population size, isolation, and fragmentation of extant populations. Moderate to high levels of inbreeding were also found, probably as consequence of the population’s genetic structuring. The conservation of the population located in the fortress would allow the preservation of all the alleles detected at species level; nevertheless, conservation of other populations, coupled with the inclusion of S. sennenii in the Spanish Catalogue of Endangered Species, would also be desirable in order to ensure the long-term survival of the species.

Diversity Patterns in the Flora of the Campo-Ma’an Rain Forest, Cameroon: Do Tree Species Tell it All?

This study describes diversity patterns in the flora of the Campo-Ma’an rain forest, in south Cameroon. In this area, the structure and composition of the forests change progressively from the coastal forest on sandy shorelines through the lowland evergreen forest rich in Caesalpinioideae with Calpocalyx heitzii and Sacoglottis gabonensis, to the submontane forest at higher elevations and the mixed evergreen and semi-deciduous forest in the drier Ma’an area. We tested whether there is a correlation between tree species diversity and diversity of other growth forms such as shrubs, herbs, and lianas in order to understand if, in the context of African tropical rain forest, tree species diversity mirrors the diversity of other life forms or strata. Are forests that are rich in tree species also rich in other life forms? To answer this question, we analysed the family and species level floristic richness and diversity of the various growth forms and forest strata within 145 plots recorded in 6 main vegetation types. A comparison of the diversity within forest layers and within growth forms was done using General Linear Models. The results showed that tree species accounted for 46% of the total number of vascular plant species with DBH ≥1 cm, shrubs/small trees 39%, climbers 14% and herbs less than 1%. Only 22% of the diversity of shrubs and lianas could be explained by the diversity of large and medium sized trees, and less than 1% of herb diversity was explained by tree diversity. The shrub layer was by far the most species rich, with both a higher number of species per plot, and a higher Shannon diversity index, than the tree and the herb layer. More than 82% of tree species, 90% of shrubs, 78% of lianas and 70% of herbaceous species were recorded in the shrub layer. Moreover, shrubs contributed for 38% of the 114 strict and narrow endemic plant species recorded in the area, herbs 29%, trees only 20% and climbers 11%. These results indicate that the diversity of trees might not always reflect the overall diversity of the forest in the Campo-Ma’an area, and therefore it may not be a good indicator for the diversity of shrubs and herbaceous species. Furthermore, this suggests that biodiversity surveys based solely on large and medium sized tree species (DBH ≥0cm) are not an adequate method for the assessment of plant diversity because other growth form such as shrubs, climbers and herbs are under-represented. Therefore, inventory design based on small plots of 0.1 ha, in which all vascular plants with DBH ≥1 cm are recorded, is a more appropriate sampling method for biodiversity assessments than surveys based solely on large and medium sized tree species.

Variation in Dispersal Traits in a Narrow-endemic Plant Species, Centaurea corymbosa Pourret. (Asteraceae)

The existence of genetic variability for dispersal is a crucial issue for organisms facing increased habitat fragmentation and climate change. We study the genetic basis and evolutionary potential for diaspore traits related to dispersal in Centaurea corymbosa. Using diaspores collected in natural conditions in four of the six extant populations of this narrow-endemic plant species and diaspores produced in a common garden experiment, we study the variation for pappus and achene sizes, and diaspore mass. Using a sample of achenes from the common garden experiment, we find that the best predictor of terminal velocity is a linear combination of pappus length, achene width, and achene weight. We find significant differences among populations for all traits in both conditions, as well as significant differences among families within population. Although the differences among populations for some traits are not exactly the same in controlled conditions compared to natural conditions, the ranking of populations according to their mean trait values is consistent in both conditions. Our study is therefore one of the first to show a correlation between phenotypic differentiation for dispersal traits in natural conditions vs. controlled conditions. We also show evidence of genetic variation for traits commonly thought to be involved in dispersal ability, suggesting the potential for evolutionary changes following environmental change and management actions.

The biology and ecology of narrow endemic and widespread plants: a comparative study of trait variation in 20 congeneric pairs

The objective of this study is to examine whether habitat, herbivory and traits related to resource acquisition, resource conservation, reproduction and dispersal differ between narrow endemic plant species and their widespread congeners. We undertook pairwise contrasts of 25 ecological characteristics and biological traits in 20 congeneric pairs of narrow endemic and widespread plant species in the French Mediterranean region. Within each pair, the two species had the same life‐form, pollination mode and dispersal mode. Endemic species differed significantly from widespread congeners for a number of attributes. Endemic species occur in habitats on steeper slopes, with higher rock cover and in lower and more open vegetation than their widespread congeners. Endemic species are significantly smaller than widespread species, but show no differences in traits related to resource acquisition (specific leaf area, leaf nitrogen content, maximum photosynthetic rate) or resource conservation (leaf dry matter content). After accounting for their smaller stature, endemic species produce fewer and smaller flowers with less stigma‐anther separation and lower pollen/ovule ratios and produce fewer seeds per plant than their widespread congeners. No consistent variation in seed mass and propagule structure was found between congeneric species. Herbivory levels did not differ between congeneric species. Ecological characteristics, notably the occupation of rocky habitats with low aboveground competition, may thus have played an important role in the differentiation of narrow endemic species in the Western Mediterranean. Morphological and ecophysiological traits of narrow endemic species indicate that they are not more stress‐tolerant than their widespread congeners. Lower investment in pollen transfer and seed production suggest that local persistence is a key feature of the population ecology of narrow endemic species.