Narrow Dynamic Range Research Articles

Reducing the dynamic range of infrared images based on block-priority equalization and compression of histograms

Objectives. The problem of reducing the dynamic range of infrared images for their reproduction on display devices with a narrow dynamic range is considered. The method of local image histogram equalization based on the integral distribution function of brightness is investigated. To transform the brightness of a pixel, this method uses an approximation of the local alignment values of the nearest blocks of pixels of original image. This in-creases the local contrast of the image, but leads to high computational complexity, which is increasing while block size decreases. The aim of the work is to reduce the computational complexity of adaptive equalization and compression of infrared image histograms while reducing their dynamic range.Methods. Image processing methods are used.Results. To reduce the computational complexity of transforming the dynamic range of infrared images, a block-priority modification of the adaptive histogram equalization method is proposed. The modification is based on the division of the set of image blocks into two subsets of high-priority and low-priority blocks depend-ing on their brightness statistical properties. When interpolating pixel values, high-priority blocks use local alignment values, and low-priority blocks use global alignment values. As a result, the total number of alignment vectors is reduced in proportion to the ratio of subsets sizes and the computational complexity of the dynamic range transformation is reduced.Conclusion. When changing the ratio of the number of high-priority blocks of infrared image pixels to the number of all blocks in the range of 0.25–0.75, the proposed algorithm is more efficient than global and adaptive histogram equalization algorithms.

In This Issue of the Journal

In This Issue of the Journal

Dual Voltage Forward Topology for High Efficiency at Universal Mains

This paper introduces a forward converter aimed at the universal mains voltages, i.e., 220–230 Vac and 115 Vac, named the ‘dual voltage forward converter’. The suggested converter has a narrow dynamic range at the universal mains voltages, which results in lower stress on devices, optimal duty cycles, and better overall efficiency. The topology comprises two primary power loops reconfigurable by additional two-state switches and a passive diode, which allows the converter to run in parallel or in series modes and increase the performance over the full universal mains range of 90–265 Vac. The utilization of the devices is better, as they experience lower voltage and current stress by supporting two optimized working points. A converter operating at 100 kHz with an output power of 75 W and output voltage of 12 Vdc was designed and tested. The results were compared with a conventional forward converter with the identical specification. The results at the low mains were similar between the converters; however, at the high mains, the efficiency improvement was between 5% and 23%.

Programmable Synthetic Upstream Activating Sequence Library for Fine-Tuning Gene Expression Levels in Saccharomyces cerevisiae.

A wide dynamic range of promoters is necessary for fine-tuning transcription levels. However, weak intensity and narrow dynamic range limit transcriptional regulation via constitutive promoters. The upstream activation sequence (UAS) located upstream of the core promoter is a crucial region that could obviously enhance promoter strength. Herein, we created a random mutagenesis library consisting of 330 different variants based on the UAS of the TDH3 promoter with an ∼37-fold dynamic range by error-prone polymerase chain reaction (PCR) and obtained strong intensity mutant UAS, which was ∼12-fold greater than the wild-type UASTDH3. Analysis of the mutant library revealed 15 strength-enhancing sites and their corresponding bases of the UASTDH3 regions, which provided the impetus for a synthetic library. The resulting 32 768 mutant UAS library was constructed by permutation and combination of the bases of the 15 enhancing sites. To characterize the library, a strength prediction model was built by correlating DNA structural features and UAS strength, which provided a model between UAS sequence and intensity. Following characterization, the UAS library was applied to precisely regulate gene expression in the production of β-carotene, proving that the UAS library would be a useful tool for gene tuning in metabolic engineering. In summary, we designed, constructed, and characterized a UAS library that facilitated precise tuning of transcription levels of target proteins.

Characterization in nonhuman primates of (R)-[18F]OF-Me-NB1 and (S)-[18F]OF-Me-NB1 for imaging the GluN2B subunits of the NMDA receptor

PurposeGluN2B containing N-methyl-D-aspartate receptors (NMDARs) play an essential role in neurotransmission and are a potential treatment target for multiple neurological and neurodegenerative diseases, including stroke, Alzheimer’s disease, and Parkinson’s disease. (R)-[18F]OF-Me-NB1 was reported to be more specific and selective than (S)-[18F]OF-Me-NB1 for the GluN2B subunits of the NMDAR based on their binding affinity to GluN2B and sigma-1 receptors. Here we report a comprehensive evaluation of (R)-[18F]OF-Me-NB1 and (S)-[18F]OF–Me-NB1 in nonhuman primates.MethodsThe radiosynthesis of (R)-[18F]OF-Me-NB1 and (S)-[18F]OF-Me-NB1 started from 18F-fluorination of the boronic ester precursor, followed by removal of the acetyl protecting group. PET scans in two rhesus monkeys were conducted on the Focus 220 scanner. Blocking studies were performed after treatment of the animals with the GluN2B antagonist Co101,244 or the sigma-1 receptor antagonist FTC-146. One-tissue compartment (1TC) model and multilinear analysis-1 (MA1) method with arterial input function were used to obtain the regional volume of distribution (VT, mL/cm3). Occupancy values by the two blockers were obtained by the Lassen plot. Regional non-displaceable binding potential (BPND) was calculated from the corresponding baseline VT and the VND derived from the occupancy plot of the Co101,244 blocking scans.Results(R)- and (S)-[18F]OF-Me-NB1 were produced in > 99% radiochemical and enantiomeric purity, with molar activity of 224.22 ± 161.69 MBq/nmol at the end of synthesis (n = 10). Metabolism was moderate, with ~ 30% parent compound remaining for (R)-[18F]OF-Me-NB1 and 20% for (S)-[18F]OF-Me-NB1 at 30 min postinjection. Plasma free fraction was 1–2%. In brain regions, both (R)- and (S)-[18F]OF-Me-NB1 displayed fast uptake with slower clearance for the (R)- than (S)-enantiomer. For (R)-[18F]OF-Me-NB1, both the 1TC model and MA1 method gave reliable estimates of regional VT values, with MA1 VT (mL/cm3) values ranging from 8.9 in the cerebellum to 12.8 in the cingulate cortex. Blocking with 0.25 mg/kg of Co101,244 greatly reduced the uptake of (R)-[18F]OF-Me-NB1 across all brain regions, resulting in occupancy of 77% and VND of 6.36, while 0.027 mg/kg of FTC-146 reduced specific binding by 30%. Regional BPND, as a measure of specific binding signals, ranged from 0.40 in the cerebellum to 1.01 in the cingulate cortex.ConclusionsIn rhesus monkeys, (R)-[18F]OF-Me-NB1 exhibited fast kinetics and heterogeneous uptake across brain regions, while the (S)-enantiomer displayed a narrower dynamic range of uptake across regions. A Blocking study with a GluN2B antagonist indicated binding specificity. The value of BPND was > 0.5 in most brain regions, suggesting good in vivo specific binding signals. Taken together, results from the current study demonstrated the potential of (R)-[18F]OF-Me-NB1 as a useful radiotracer for imaging the GluN2B receptors.

Age-dependent modulation of short-term neuronal dynamics in the dorsal and ventral rat hippocampus.

Brain aging is associated with alterations in the behavioral capacity to process information, due to mechanisms that are still largely unclear. Short-term neuronal activity dynamics are basic properties of local brain networks profoundly involved in neural information processing. In this study, we investigated the properties of short-term changes in excitatory synaptic transmission and neuronal excitation in the CA1 field of dorsal and ventral hippocampal slices from young adult and old rats. We found that short-term synaptic plasticity (i.e. short-term dynamics of input to CA1 circuit) does not significantly differ between young and old dorsal or ventral hippocampus. However, short-term dynamics of hippocampal output differ markedly between young and old rats. Notably, age-dependent alterations in short-term neuronal dynamics were detected mainly in the dorsal hippocampus. Thus, the dorsal hippocampus of young rats can detect and facilitate transmission of 1-30 Hz input and depress transmission of higher-frequency input. In contrast, the old dorsal hippocampus appears unable to transmit information in a frequency-dependent discriminatory manner. Furthermore, the amplification of steady-state output at frequencies < 40 Hz is considerably lower in the old than the young dorsal hippocampus. The old ventral hippocampus did not show major alterations in short-term processing of neural information, though under conditions of intense afferent activation, neuronal output of the ventral hippocampus is depressed at steady-state more in old than in young rats. These results suggest that aging is accompanied by alterations in neural information processing mainly in the dorsal hippocampus, which displays a narrower dynamic range of frequency-dependent transient changes in neuronal activity in old compared with young adult rats. These alterations in short-term dynamics may relate to deficits in processing ongoing activity seen in old individuals.

Antibody-Free Quantification of Serum Chromogranin A by Targeted Mass Spectrometry.

Chromogranin A (CgA) is a 48 kDa protein that serves as a diagnostically sensitive, but nonspecific, serum biomarker for neuroendocrine tumors. Immunoassays for CgA are not standardized and have a narrow dynamic range, which requires dilution of concentrated specimens. We developed and validated an antibody-free, liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based method for CgA without these limitations. CgA was extracted from serum using a mixed-mode anion exchange solid-phase extraction plate, digested with trypsin, and analyzed by LC-MS/MS using well-characterized CgA calibration standards. After validation, the mass spectrometry method was compared with the CISBIO immunoassay using 200 serum specimens previously submitted for CgA analysis. Specimens with discordant results were reanalyzed by high-resolution mass spectrometry- (HRMS) -based methods to assess the contribution of truncated and post-translationally modified forms of CgA. The assay had a linear range of 50 to 50 000 ng/mL, recoveries between 89% and 115%, and intra- and interassay imprecision <10%. LC-MS/MS assay results showed a Pearson's correlation of r = 0.953 with the CISBIO immunoassay, with CgA values being a mean 2- to 4-fold higher. Concordance for CgA between the 2 assays was 80.9% (95% CI 72.8%-89.2%), showing substantial agreement. Truncation and posttranslational modification, including 2 phosphorylation sites that had not been previously observed or predicted to our knowledge, did not appear to contribute directly to discordance between the 2 assays. Quantification of CgA by LC-MS/MS provides an analytically sensitive and reproducible alternative to commercially available immunoassays.

Panspermia in a Milky Way–like Galaxy

Abstract We study the process of panspermia in Milky Way–like galaxies by modeling the probability of successful travel of organic compounds between stars harboring potentially habitable planets. To this end, we apply the modified habitability recipe of Gobat &amp; Hong to a model galaxy from the McMaster Unbiased Galaxy Simulations suite of zoom-in cosmological simulations. We find that, unlike habitability, which only occupies a narrow dynamic range over the entire galaxy, the panspermia probability can vary by orders of magnitude between the inner (R, b = 1–4 kpc) and outer disk. However, only a small fraction of star particles have very large values for the panspermia probability and, consequently, the fraction of star particles where the panspermia process is more effective than prebiotic evolution is much lower than from naïve expectations based on the ratio between the panspermia probability and natural habitability.

Fast structured illumination microscopy with a large dynamic measurement range.

Fast structured illumination microscopy plays an important role in micro-nano detection due to the features of high accuracy, high efficiency, and excellent adaptability. The existing method utilizes the linear region of the axial modulation response curve (AMR), and by building the relationship between the modulation and the real height, achieves topography recovery. However, the traditional method is limited to narrow dynamic measurement range due to the linear region of the AMR being very short. In this paper, a double-differential fast structured illumination microscopy (DDFSIM) is proposed. By introducing two additional detectable branches for building the double-differential axial modulation response curve (DDAMR), the proposed method can obtain a large dynamic measurement range. In the measurement, three charge-coupled devices are respectively placed in and behind and before the focal plane to generate three axial modulation response curves. Three AMRs are used to set up the DDAMR, which has a large dynamic measurement range. Through simulation and experimental verification, the measurement range of DDFSIM is twice that of the conventional method under the same system parameters.

Reflective Parametric Frequency-Selective Limiters With Sub-dB Loss and μWatts Power Thresholds

This article describes the design methodology to achieve reflective diode-based parametric frequency selective limiters (pFSLs) with low power thresholds ($P_{th}$) and sub-dB insertion-loss values ($IL^{s.s}$) for driving power levels ($P_{in}$) lower than $P_{th}$. In addition, we present the measured performance of a reflective pFSL designed through the discussed methodology and assembled on a FR-4 printed circuit board (PCB). Thanks to its optimally engineered dynamics, the built pFSL can operate around $\sim$2.1 GHz while exhibiting record-low $P_{th}$ (-3.4 dBm) and $IL^{s.s}$ (0.94 dB) values. Furthermore, while the pFSL can selectively attenuate undesired signals with power ranging from -3.4 dBm to 13 dBm, it provides a strong suppression level (IS > 12.0 dB) even when driven by much higher $P_{in}$ values approaching 28 dBm. Such measured performance metrics demonstrate how the unique nonlinear dynamics of parametric-based FSLs can be leveraged through components and systems compatible with conventional chip-scale manufacturing processes in order to increase the resilience to electromagnetic interference (EMI), even of wireless radios designed for a low-power consumption and consequently characterized by a narrow dynamic range.

Conditional preparation of non-Gaussian quantum optical states by mesoscopic measurement

Non-Gaussian states of an optical field are important as a proposed resource in quantum information applications. While conditional preparation is a highly successful approach to preparing such states, their quality is limited by detector non-idealities such as dead time, narrow dynamic range, limited quantum efficiency and dark noise. Mesoscopic photon counters, with peak performance at higher photon number, offer many practical advantages over single-photon level conditioning detectors. Here we propose a novel approach involving displacement of the ancilla field into the regime where mesoscopic detectors can be used. We explore this strategy theoretically and present simulations accounting for experimental non-idealities such as loss and amplification noise, showing that precise photon-number resolution is not necessary to herald highly nonclassical states. We conclude that states with strong Wigner negativity can be prepared at high rates by this technique under experimentally attainable conditions.

Development of a Core Outcome Set for Therapeutic Studies in Eosinophilic Esophagitis (COREOS): An International Multidisciplinary Consensus

Development of a Core Outcome Set for Therapeutic Studies in Eosinophilic Esophagitis (COREOS): An International Multidisciplinary Consensus

Development and Characterization of a Highly Sensitive NanoLuciferase-Based Immunoprecipitation System for the Detection of Anti-Influenza Virus HA Antibodies.

Antibody detection is crucial for monitoring host immune responses to specific pathogen antigens (Ags) and evaluating vaccine efficacies. The luciferase immunoprecipitation system (LIPS) was developed for sensitive detection of Ag-specific antibodies in sera from various species. In this study, we describe NanoLIPS, an improved LIPS assay based on NanoLuciferase (NLuc), and employ the assay for monitoring antibody responses following influenza virus infection or vaccination. We generated recombinant influenza virus hemagglutinin (HA) proteins tagged with N-terminal (N-NLuc-HA) or C-terminal (C-NLuc-HA) NLuc reporters. NLuc-HA yielded an at least 20-fold higher signal-to-noise ratio than did a LIPS assay employing a recombinant HA-Gaussia princeps luciferase (GLuc) fusion protein. NanoLIPS-based detection of anti-HA antibodies yielded highly reproducible results with a broad dynamic range. The levels of antibodies against C-NLuc-HA generated by mice vaccinated with recombinant vaccinia virus DIs strain expressing an influenza virus HA protein (rDIs-HA) was significantly correlated with the protective effect elicited by the rDIs-HA vaccine. C-NLuc-HA underwent glycosylation with native conformations and assembly to form a trimeric structure and was detected by monoclonal antibodies that detect conformational epitopes present on the globular head or stalk domain of HA. Therefore, NanoLIPS is applicable for evaluating vaccine efficacy. We also showed that C-NLuc-HA is applicable for detection of HA-specific antibodies in sera from various experimental species, including mouse, cynomolgus macaque, and tree shrew. Thus, NanoLIPS-based detection of HA offers a simple and high-sensitivity method that detects native conformational epitopes and can be used in various experimental animal models.IMPORTANCE Influenza virus HA-specific antibodies can be detected via the hemagglutination inhibition (HI) assay, the neutralization (NT) assay, and the enzyme-linked immunosorbent assay (ELISA). However, these assays have some drawbacks, including narrow dynamic range and the requirement for large amounts of sera. As an alternative to an ELISA-based method, luciferase immunoprecipitation system (LIPS) was developed. We focused on NanoLuciferase (NLuc), which has a small size, higher intensity, and longer stability. In this study, we developed a technically feasible and highly sensitive method for detecting influenza virus-specific antibodies using a NLuc-tagged recombinant HA protein produced in mammalian cells. HA with a C-terminal NLuc extension (C-NLuc-HA) was glycosylated and formed trimeric complexes when expressed in mammalian cells. Furthermore, C-NLuc-HA was recognized not only by monoclonal antibodies that bind to the globular head domain but also by those that bind to the stalk domain. We also demonstrated that the data obtained by this assay correlate with the protection of an experimental vaccine in animal models.

Calcium Signal Profiles in Vascular Endothelium from Cdh5-GCaMP8 and Cx40-GCaMP2 Mice

Introduction: Studies in Cx40-GCaMP2 mice, which express calcium biosensor GCaMP2 in the endothelium under connexin 40 promoter, have identified the unique properties of endothelial calcium signals. However, Cx40-GCaMP2 mouse is associated with a narrow dynamic range and lack of signal in the venous endothelium. Recent studies have proposed many GCaMPs (GCaMP5/6/7/8) with improved properties although their performance in endothelium-specific calcium studies is not known. Methods: We characterized a newly developed mouse line that constitutively expresses GCaMP8 in the endothelium under the VE-cadherin (Cdh5-GCaMP8) promoter. Calcium signals through endothelial IP3 receptors and TRP vanilloid 4 (TRPV4) ion channels were recorded in mesenteric arteries (MAs) and veins from Cdh5-GCaMP8 and Cx40-GCaMP2 mice. Results: Cdh5-GCaMP8 mice showed lower baseline fluorescence intensity, higher dynamic range, and higher amplitudes of individual calcium signals than Cx40-GCaMP2 mice. Importantly, Cdh5-GCaMP8 mice enabled the first recordings of discrete calcium signals in the intact venous endothelium and revealed striking differences in IP3 receptor and TRPV4 channel calcium signals between MAs and mesenteric veins. Conclusion: Our findings suggest that Cdh5-GCaMP8 mice represent significant improvements in dynamic range, sensitivity for low-intensity signals, and the ability to record calcium signals in venous endothelium.

Reagentless Affimer- and antibody-based impedimetric biosensors for CEA-detection using a novel non-conducting polymer

Polyoctopamine (POct), an amine-functionalised non-conducting polymer, as the transducer layer in an electrochemical biosensor, is presented. This polymer offers versatile covalent coupling either through thiol linker conjugation, carboxyl or aldehyde functional groups without the requirement of pre- or post-surface activation. The colorectal cancer biomarker carcinoembryonic antigen (CEA) was selected as the target analyte, whilst an antibody and a synthetic binding protein, an Affimer, were used as distinct bioreceptors to demonstrate the versatility of polyoctopamine as a transducer polymer layer for oriented immobilisation of the bioreceptors. The electrodeposited polymer layer was characterised using cyclic voltammetry, electrochemical impedance spectroscopy, and on-sensor chemiluminescent blotting. The performance of optimised POct-based biosensors were tested in spiked human serum. Results showed that the electropolymerisation of octopamine on screen printed gold electrode generates a thin polymer film with low resistance. Close proximity of the immobilised bioreceptors to the transducer layer greatly enhanced the sensitivity detection. The sensitivity of the smaller monomeric bioreceptor (Affimer, 12.6 kDa) to detect CEA was comparable to the dimeric antibody (150 kDa) with limit of detection at 11.76 fM which is significantly lower than the basal clinical levels of 25 pM. However, the Affimer-based sensor had a narrower dynamic range compared to the immunosensor (1–100 fM vs. 1 fM – 100 nM, respectively). All electrochemical measurements were done in less than 5 min with small sample volumes (10 μl). Hence, polyoctopamine features a simple fabrication of impedimetric biosensors using amine-functionalisation technique, provides rapid response time with enhanced sensitivity and label-free detection.

Protein Quantification Using the "Rapid Western Blot" Approach.

For the quantification of certain proteins of interest within a complex sample, Western blot analysis is the most widely used method. It enables detection of a target protein based on the use of specific antibodies. However, the whole procedure is often very time-consuming. Nevertheless, with the development of fast blotting systems and further development of immunostaining methods, a reduction of the processing time can be achieved. Major challenges for the reliable protein quantification by Western blotting are adequate data normalization and stable protein detection. Usually, normalization of the target protein signal is performed based on housekeeping proteins (e.g., glyceraldehyde 3-phosphate dehydrogenase, ß-actin) with the assumption that those proteins are expressed constitutively at the same level across experiments. However, several studies have already shown that this is not always the case making this approach suboptimal. Another strategy uses total protein normalization where the abundance of the target protein is related to the total protein amount in each lane. This approach is independent of a single loading control, and precision of quantification and reliability is increased. For Western blotting several detection methods are available, e.g., colorimetric, chemiluminescent, radioactive, fluorescent detection. Conventional colorimetric staining tends to suffer from low sensitivity, limited dynamic range, and low reproducibility. Chemiluminescence-based methods are straightforward, but the detected signal does not linearly correlate to protein abundance (from protein amounts >5μg) and have a relatively narrow dynamic range. Radioactivity is harmful to health. To overcome these limitations, stain-free methods were developed allowing the combination of fluorescent standards and a stain-free fluorescence-based visualization of total protein in gels and after transfer to the membrane. Here, we present a rapid Western blot protocol, which combines fast blotting using the iBlot system and fast immunostaining utilizing ReadyTector® all-in-one solution with the Smart Protein Layers (SPL) approach.

A 3D-cascade-microlens optofluidic chip for refractometry with adjustable sensitivity.

Refractive index (RI) sensing as a label-free and non-invasive method has been playing an important role in industrial metrology, biochemical detection, and environmental analysis. Due to the combined advantages of microoptics and microfluidics, optofluidic RI sensors have attracted growing interest. Despite a variety of prototypes of optofluidic RI sensors, comprehensive improvement in sensitivity, detection range, fabrication procedures and cost can still bring substantial benefits to the field. In this work, we fabricated a 3D-cascade-microlens optofluidic chip (3DCMOC) for RI sensing. Two-photon stereolithography was employed to fabricate the chip mold, with which the 3DCMOC could be easily manufactured via mold replication. By virtue of integrating four detection channels configured with different numbers (1, 3, 5, and 7) of cascaded microlenses within the 3DCMOC, adjustable sensitivity for RI sensing has been demonstrated through measuring standard sucrose solutions. It was found that the seven-microlens configuration achieved an excellent sensitivity (mean: 21 ± 5 AU·RIU (refractive index unit)-1) and resolution (mean: 3.8 × 10-5 ± 0.9 × 10-5 RIU) at a cost of a narrow linear dynamic range (LDR, 1.3326-1.3548). In contrast, the single-microlens configuration led to an extended LDR (1.3326-1.5120 tested) despite the lower sensitivity (mean: 2.6 ± 0.2 AU·RIU-1) and resolution (mean: 1.5 × 10-4 ± 0.1 × 10-4 RIU). Furthermore, the use of the 3DCMOC was investigated via real-time salinity sensing and analysis of urine specific gravity.

Non-scanning wide range multi-wavelength imaging temperature measurement technology

Multi-wavelength temperature measurement technology is an advanced radiation temperature measurement technology, which assumes a functional relationship between emissivity and spectrum. The measured multi-wavelength radiation signals and the function relationship between emissivity and spectral is used to obtain the true temperature and emissivity of the target. Multi-wavelength imaging temperature measurement technology detects the multi-wavelength radiation image information of the target and inversely calculates the temperature field distribution of the target. Aiming at the inadequacy of color CCD-based multi-wavelength imaging temperature measurement, such as adaptability of the nonlinear spectral emissivity model, and the narrow dynamic range of temperature measurement, and so on, a four-wavelength non-scanning imaging temperature measurement method based on non-equal ratio filter color splitting at the diaphragm was proposed. This method could effectively compress the band imaging bandwidth to form four narrow band imaging detections, and was suitable for wide dynamic range temperature field measurement of nonlinear spectral emissivity model targets. A four-wavelength imaging thermometer was developed according to the proposed temperature measurement method. The thermometer was mainly composed of a window, a neutral optical attenuator, a four-color filter, an optical objective lens, a visible/near infrared integrated sensor, a measurement control unit, and software. The real temperature distribution of the target was obtained according to the multi-wavelength warming algorithm by the thermometer software. The target high-temperature field (800-2500 ℃) was tested under laser heating conditions. The comparison between the measurement result and the thermocouple data shows that the error is less than 1%, and the method has higher accuracy and better dynamic range adaptability.

In-Bed Biosignal Acquisition From Conventional Differential Pressure Sensor Based on Thermal Flow Principle

We propose a non-invasive pneumatic biosensing method that measures the pressure on a tube placed under a bed cushion. Although similar methods use microphones for pressure sensing, the high sensitivity of microphones may cause problems such as saturation, slow recovery from saturation, narrow dynamic range, and low signal-to-noise (S/N) ratio. However, the proposed method uses a differential pressure sensor based on the thermal flow principle which has been developed and commercialized recently. We derive theoretical models of both microphone and thermal flow measurements to show that the proposed method can solve the problems of methods based on microphones. Basic experimental results validate both models providing the time constant (65 s), saturation range (-80-+40 Pa), noise level (4.02 Pa), dynamic range (32 dB) and S/N ratio (21 dB) of the low-frequency microphone developed for biosignal measurement and the superior time constant (0.012 s), saturation range (-500-+500 Pa), noise level (0.82 Pa), dynamic range (62 dB) and S/N ratio (56 dB) of the proposed method based on a differential pressure sensor. Furthermore, the effective S/N ratio in the measurement of heart beat signal was also compared between the low-frequency microphone and the proposed method. The former showed S/N ratio (22.1 dB), and the latter showed S/N ratio (28.1dB).

A lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe.

The fission yeast Schizosaccharomyces pombe is an excellent model organism for the study of eukaryotic cellular physiology. The organism is genetically tractable and several tools to study the functions of individual genes are available. One such tool is regulatable gene expression and overproduction of proteins. Limitations of currently available overexpression systems include delay in expression after induction, narrow dynamic range, and system-wide changes due to induction conditions. Here I describe a new long noncoding RNA (lncRNA)-regulated, thiamine-inducible expression system that integrates lncRNA-based transcriptional interference at the fission yeast tgp1 promoter with the fast repression kinetics of the thiamine-repressible nmt1 promoter. This hybrid system has rapid induction kinetics, broad dynamic range, and tunable expression via thiamine concentration. The lncRNA-regulated thiamine-inducible system will be advantageous for the study of individual genes and for potential applications in the production of heterologous proteins in fission yeast.