This study aimed to determine the effects of plant spacing on some morphological traits and yields of 5 hot pepper lines. The trial was laid out in a 5 × 3 factorial in randomized complete block design with 4 replications. Five pepper lines (ICPN16#3, ICPN16#4, ICPN16#6, ICPN16#7, and ICPN16#9) and 3 plant spacing (70 cm × 30 cm, 70 cm × 40 cm and 70 cm × 50 cm) were used. The results revealed that the closer plant spacing of 70 cm × 30 cm produced tall plants with ICPN16#4 line being the tallest. In addition, line ICPN16#4 produced narrow canopy with more branches, whilst ICPN16#9 (a short line) produced more branches with medium spread canopy. Plant spacing of 70 cm × 50 cm resulted in more spread canopy and number of branches. Closer spacing gave higher fruit yield compared to wider spacing. However, wider spacing gave the largest fruit size. Key words: Lines, spacing, canopy, plant height, branch, interaction.