In this work, we report a novel well-dispersed palladium nanoparticles (PdNPs)/nickel-phosphorus nanosheets (Ni-P NSs) synthesized by combination of red phosphorous phosphorization and simple electrodeposition technique. The obtained PdNPs/Ni-P nanosheets framework with large electrochemical active surface would not only exhibit much superior electrocatalytic activity (180.5±3.07mAcm−2) toward glucose oxidation reaction, but also represent a wide linear range (2μM−4.65mM) and high sensitive detection of glucose (242.5±3.28μAmM−1cm−2) as non-enzymatic glucose sensor. The three- dimensional (3D) PdNPs/Ni-P nanosheets have a promising prospect as a novel kind of highly active nanocatalyst and non-enzymatic electrochemical sensor.