Nanoscale Characterization Research Articles

A HR-TEM study on two generations of magnetite from the Alemao IOCG deposit, Carajás, Brazil: Implication for Fe-Cu mineralization

Multiple generations of magnetite are common in many magmatic-hydrothermal Fe deposits, which can be formed by different mechanisms. This study investigates magnetite from the Alemao IOCG deposit (Brazil) characterized by inclusion-rich cores (Mag-1) and inclusion-poor rims (Mag-2), using electron probe microanalyzer and high-resolution transmission electron microscopy (HRTEM), to understand its formation mechanism and implications for Fe and Cu mineralization. Electron probe microanalysis shows that Mag-1 has Si and Mg contents of 0.28–1.15 wt% and 0.11–0.49 wt%, respectively, higher than Mag-2. Mineral nanoinclusions of minnesotaite, chlorite, quartz, and minor Ti-rich magnetite in the Mag-1 account for the relatively high Si and Mg in the Mag-1, as measured by electron probe microanalysis. The crystal orientation of nanoinclusions relative to the host Mag1 indicates formation by growth entrapment, where they crystallized from boundary layer supersaturated fluids under non-equilibrium conditions.Identification of a reaction front, spatial coupling of parent and product phases at micro- and nano-meter scales support the formation of Alemao Mag2 by coupled dissolution and reprecipitation (CDR). Variations in fluid composition rather than fluctuations in conditions such as temperature and oxygen fugacity are the factors inducing CDR. A conceptual model is proposed for the formation of Alemao magnetite where Fe-rich fluids formed inclusion-rich primary magnetite and CDR reactions modified the texture and chemical composition of primary magnetite to form a core rich, and a rim poor, in nanoinclusions. During CDR, impurities of Si, Ca, Al, Mn, and Mg and nanoinclusions are removed from the primary magnetite and formed product magnetite with higher Fe. The progressive enrichment of Cu from Mag-1 to Mag-2 during CDR indicates that a Cu-rich fluid related to the Cu-Au mineralization is responsible the formation of Cu-rich Mag-2 and chalcopyrite. Therefore, micron- to nano-scale characterization of magnetite with multiple generations can provide important constraints on fluid evolution in ore system.

Effect of annealing treatment on microstructure evolution and deformation behavior of 304 L stainless steel made by laser powder bed fusion

This paper focuses on the microstructural evolution of 304 L austenitic stainless steel (SS) manufactured by laser powder bed fusion (LPBF) after stress-relieving annealing (650 °C) and solution annealing (1050 °C). Multiple advanced characterizations were adopted to disclose the microstructural characteristics and investigate the annealing-driven dislocation migration process. At 650 °C, the dislocation density of cellular walls decreased slightly, associated with a slight decrease of strength. At 1050 °C, the dislocations of cellular walls migrated to more energetically favorable regions, forming subgrain boundaries with higher dislocation density and resulting in a strength-ductility trade-off. The temperature of 1050 °C could slightly increase the recrystallization volume fraction and induce the coalescence of multi-oriented fine-grained tribes into single-oriented grains. The nano-scale characterization indicated that the as-built samples and annealed samples at 650 °C contained the square lattice distortion networks composed of orthogonal strain ripples. However, after annealing at 1050 °C, only unidirectional strain ripples in square lattice distortion networks were retained due to unidirectional dislocation migration. Direct experimental results were provided that the local misorientation ranges of cellular interior, cellular walls, and newly formed subgrain boundaries were <0.2°, 0.2°-0.5°, and 0.5°-2°, respectively. After tensile deformation, interacting deformation twins occurred in <111> and 〈101〉 oriented grains of as-built and annealed specimens at 650 °C, while the twins occurred in all oriented grains of annealed specimens at 1050 °C due to the disappearance of cellular substructure and the increase of tensile elongation. This work yields new insights into misorientation across the cellular walls, dislocation migration process during annealing, strengthening mechanisms, and work hardening behaviors, which can be used to design and optimize future annealing routines for LPBF materials.

Nanoscale engineering of gold particles in 18th century Böttger lusters and glazes

SignificanceThe exploration of gold-based colorants in glass and glazes led Nobel Laureate Richard Zsigmondy to the study of colloids, and to the development, with Henry Siedentopf, of the earliest microscopes capable of resolving such small length scales. Zsigmondy's studies were preceded by alchemical investigations starting in the 17th century that yielded the gold-based Purple of Cassius, and experiments in the early 18th century resulting in an unusual purple iridescent porcelain overglaze, called Böttger luster, at the Meissen Manufactory. We discuss the first nano-scale characterization of Böttger luster, its successful replication, and propose an explanation for its optical properties based on the physics of scattering and interference of nanoparticle arrays.

Synthesis and Nanoscale Characterization of Hierarchically Assembled Molecular Nanosheets

AbstractChemical functionalization of molecular 2D materials toward the assembly of hierarchical functional nanostructures is of great importance for nanotechnology including areas like artificial photocatalytic systems, nanobiosensors, or ultrafiltration. To achieve the desired functionality of 2D materials, these need to be characterized down to the nanoscale. However, obtaining the respective chemical information is challenging and generally requires the application of complementary experimental techniques. Here, the synthesis and chemical characterization of hierarchically assembled molecular nanosheets based on ≈1 nm thin, molecular carbon nanomembrane (CNM) and covalently grafted, single‐molecule layer cobalt(III) catalysts for the light‐driven hydrogen evolution reaction (HER) is demonstrated. X‐ray photoelectron spectroscopy (XPS) and tip‐enhanced Raman spectroscopy (TERS) to access both the transversal and longitudinal chemical information of the synthesized nanosheets with nanometer resolution are employed. TERS and XPS data provide detailed information on the average and local surface distribution of the catalyst as well as mechanistic details of the grafting reaction. The proposed approach represents a general route toward a nanoscale structural analysis for a variety of molecular 2D materials—a rapidly growing materials class with broad prospects for fundamental science and applications.

Harnessing Fe3O4 Screen-Printed Modified Electrode Sensor for Detecting Epinephrine in Buff Orpington Rooster and Rhodes Island White Broiler

Nano-scale detection and characterization of neurotransmitters from real samples is a novel analytical technique with multiple applications in the field of nano-biotechnology. This in-situ electrochemical sensing tool has growing advantages of high reproducibility, rapid response, superior sensitivity, selectivity, accuracy, and miniaturization. A screen-printed iron oxide (Fe3O4) modified electrode was harnessed in this study for detecting epinephrine (EP), a chemical messenger or signalling neuro transmitting molecule, from two breeds of chickens. The fabricated sensor was used for the analysis of EP in the real and unspiked samples. UV-visible spectroscopy, Fourier-Transform infrared spectroscopy (FT-IR), Transmission Electron Microscopy (TEM), and Scanning Electron Microscopy (SEM) were used for characterizing the surface of nanoparticles prior to modification of screen-printed silver electrode (SPSE). The XRD diffractogram of Fe3O4 nanoparticles showed peaks at 30.1°, 35.7°, 43.3°, 53.9°, 57.5°, and 63.0°, corresponding to Miller indices of 220, 311, 400, 422, 511, and 440, respectively. This diffraction pattern indicates that the Fe3O4 nanoparticles have a spinel structure. Simultaneous detection of EP in the presence of ascorbic acid was obtained from Fe3O4 electrode. Further result shows a corresponding rise in oxidation peak current (Ipa) of EP with an increase in its concentration and scan rate of 25–400 mVs−1 confirming catalytic properties of the modified electrode towards EP. Our findings demonstrate that the fabricated sensor used for detecting EP in blood serum, breast muscle, and visceral organs of both chicken breeds produced better recovery.

Gold Nanoparticles Green-Synthesized by the Suaeda japonica Leaf Extract and Screening of Anti-Inflammatory Activities on RAW 267.4 Macrophages

Biosynthesis of gold nanoparticles from medicinal plants has become a modern strategy in biomedical research based on their exclusive properties, including specific targeting, lower toxicity, and biocompatibility. In this study, gold nanoparticles, reduced by the Suaeda japonica leaf extract, were promptly validated by UV–visible (UV–Vis) spectroscopy at 548 nm. No additional reducing agents were needed in this kind of a reduction reaction, which provided evidence of green synthesis. Dynamic light scattering (DLS), energy-dispersive X-ray spectroscopy (EDX), field-emission transmission electron microscopy (FE-TEM), selected area electron diffraction (SAED), and X-ray diffraction (XRD) analyses were used to illustrate the nanoscale characterization of S. japonica gold nanoparticles (Sj-AuNps). Furthermore, the cytotoxicity effect of Sj-AuNps against the RAW 264.7 cell line was determined by performing an MTT assay. We also investigated Sj-AuNps’ anti-inflammatory properties in LPS-induced murine macrophages. These nanoparticles reduced the generation of nitric oxide (NO) and prostaglandin E2 (PGE2) and repressed the expression of the LPS-stimulated inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) genes. This study presents a significant biomedical application of S. japonica AuNps. The anti-inflammatory capabilities of Sj-AuNps underline their potential as possible options for suppressing inflammation-mediated diseases.

Near-field radiation analysis and thermal contact radius determination in the thermal conductivity measurement based on SThM open-loop system

With the rapid development of materials science, plenty of materials with micro-nano structures are emerging in various fields due to their outstanding physical properties. In order to fulfill the needs of micro-nano-scale thermal measurement, a series of thermophysical characterization methods have been evolving. In this article, a measurement method based on the Scanning Thermal Microscopy open-loop system is proposed to realize nano-scale thermal conductivity characterization. Both forward and backward thermal contact radius are measured. The heat transfer tunnel raised by the near-field radiation is calculated to be 1.73 × 10−14 W/K as the upper limit, which can be neglected compared to the total value. The total thermal conductance between tip and sample was calibrated as 1.8 × 10−6 W/K by several standard bulk samples, and thermal conductivity varies from 0.28 to 237 Wm−1 K−1.

Nanoscale Visualization of Electrochemical Activity at Indium Tin Oxide Electrodes.

Indium tin oxide (ITO) is a popular electrode choice, with diverse applications in (photo)electrocatalysis, organic photovoltaics, spectroelectrochemistry and sensing, and as a support for cell biology studies. Although ITO surfaces exhibit heterogeneous local electrical conductivity, little is known as to how this translates to electrochemistry at the same scale. This work investigates nanoscale electrochemistry at ITO electrodes using high-resolution scanning electrochemical cell microscopy (SECCM). The nominally fast outer-sphere one-electron oxidation of 1,1′-ferrocenedimethanol (FcDM) is used as an electron transfer (ET) kinetic marker to reveal the charge transfer properties of the ITO/electrolyte interface. SECCM measures spatially resolved linear sweep voltammetry at an array of points across the ITO surface, with the topography measured synchronously. Presentation of SECCM data as current maps as a function of potential reveals that, while the entire surface of ITO is electroactive, the ET activity is highly spatially heterogeneous. Kinetic parameters (standard rate constant, k0, and transfer coefficient, α) for FcDM0/+ are assigned from 7200 measurements at sites across the ITO surface using finite element method modeling. Differences of 3 orders of magnitude in k0 are revealed, and the average k0 is about 20 times larger than that measured at the macroscale. This is attributed to macroscale ET being largely limited by lateral conductivity of the ITO electrode under electrochemical operation, rather than ET kinetics at the ITO/electrolyte interface, as measured by SECCM. This study further demonstrates the considerable power of SECCM for direct nanoscale characterization of electrochemical processes at complex electrode surfaces.

The Early-Stage Corrosion of Copper Materials in Chloride and Sulfide Solutions: Nanoscale Characterization and The Effect of Microstructure

The microstructures of copper (Cu) materials were investigated by electron backscatter diffraction, showing that electrodeposited (ED) Cu has a homogenous polycrystalline microstructure, while cold spray (CS) Cu has a heterogeneous microstructure with varying grain size, pores, and interfacial splat regions. The corrosion rate was examined by corrosion potential (ECORR) and polarization resistance (Rp) measurements on Cu specimens in solutions containing 0.1 M NaCl + 1 × 10−3 M Na2S. Although the as sprayed CS-Cu was the least corrosion resistant, the corrosion rate of the heat-treated CS-Cu was similar to that of the ED-Cu and wrought Cu (SKB-Cu). Electrochemical behaviours of Cu materials were investigated by either a potentiodynamic scan or a potentiostatic polarization at a more positive potential (E > ECORR) for various experiment durations up to 4 h, showing that the heat-treated CS-Cu, SKB-Cu and ED-Cu exhibited very similar behaviour while the as sprayed CS-Cu showed erratic behavior consistent with a variable surface reactivity. Nanoscale scanning transmission electron microscopy analysis has been performed at the cross-section of an anodically-oxidized CS-Cu specimen, revealing a two-layer film structure, mostly composed of Cu sulfide, with a minor diffusion of sulfur in the local area of an interfacial splat boundary tip.

Preparation and laser damage mechanism of stray-light-absorbing thin films in high-energy laser systems

Surface modification is an important way to improve the surface performance of materials, especially for solving the problem of secondary pollution by stray-light-induced damage of aluminum alloy frames in high-energy laser systems. Preparation of aluminum alloy surface-modified functional films and determining the mechanism of system stray light irradiation of the films are of great significance, and they ensure that inertial confinement fusion (ICF) generates clean and sustainable energy. In this study, high-energy-system stray-light-absorbing films were prepared by anodization and electrolysis, followed by microscale and nanoscale characterization and irradiation experiments using single-pulse lasers with different energy densities. The morphologies of the specimens after irradiation were characterized and the damage mechanism of the functional film was investigated. On this basis, a dynamic thermal-mechanical direct coupling model was developed based on the ABAQUS user subroutine/Python for the process of laser irradiation of AA6061, AA6061/Al2O3, and AA6061/Al2O3/SiO2. The results indicated that stray-light-absorbing films significantly increase the stray-light absorptivity and effectively reduce the laser irradiation damage of AA6061, thus improving the accuracy and stability of the system.

Tip-Enhanced Photoluminescence of Freestanding Lateral Heterobubbles.

Two-dimensional (2D) semiconducting materials have promising applications in flexible optoelectronics, nanophotonics, and sensing based on the broad tunability of their optical and electronic properties. 2D nanobubbles form exciton funnels due to localized strain that can be used as local emitters for information processing. Their nanoscale optical characterization requires the use of near-field scanning probe microscopy (SPM). However, previous near-field studies of 2D materials were performed on SiO2/Si and metallic substrates using the plasmonic gap mode to increase the signal-to-noise ratio. Another challenge is the deterministic control of bubble size and location. We addressed these challenges by investigating the photoluminescence (PL) signals of freestanding monolayer lateral WSe2-MoSe2 heterostructures under the influence of strain exerted by a plasmonic SPM tip. For first time, we performed tip-enhanced PL imaging of freestanding 2D materials and studied the competition between the PL enhancement mechanisms by nanoindentation as a function of the tip-sample distance. We observed the tunability of PL as a function of bubble size, which opens new possibilities to design optoelectronic nanodevices.

Kelvin Probe Microscopy Investigation of Poly-Octylthiophene Aggregates.

Conductive polymers have fundamental relevance as well as novel technological applications in the organic optoelectronics field. Their photophysical and transport properties strongly depend on the molecular arrangement, and nanoscale characterization is needed to fully understand the optoelectronic processes taking place in organic devices. In this work, we study the electrostatic properties of poly-3-octylthiophene isolated structures: disordered low-packed polymer chains and crystalline layered lamellar assemblies. We characterize the electronic ground state using Kelvin probe microscopy. This allows us to resolve a rich variety of surface potential regions that emerge over the different polymer structures. These SP regions are correlated with different molecular aggregates.

Evidence of One-Dimensional Channels in Hydrogen-Bonded Organic Porous Thin Films Fabricated at the Air/Liquid Interface.

Visualization of periodically aligned pores in organic frameworks is a key to the understanding of their structural control. Comparing to monolayer-thick self-assembled molecular networks, real-space nanoscale characterization of thicker films, especially obtaining information on the stacking manner of molecules is challenging. Here, we report an atomic force microscopy study of hydrogen-bonded thin films fabricated at the air/liquid interface. The presence of one-dimensional channels is evidenced by resolving honeycomb structures over the films with the thickness variation of more than several nanometers. We also demonstrate that the film thickness can be controlled by the ratio of mixed solvent rather than the surface pressure during the fabrication at the air/liquid interface.

Applicability of coherent x-ray diffractive imaging to ferroelectric, ferromagnetic, and phase change materials

Rapid development in the field of ferroelectric and magnetic materials has attracted much interest in the past decade. The underlying mechanisms of the fundamental phenomenon of phase transitions in these materials are extremely important in understanding their physical properties and their potential technological applications. Therefore, it is vital for the advancement of high-resolution versatile imaging techniques that enable high-throughput and nano-scale characterization in the nano-crystals and electronic devices. X-ray based imaging techniques such as Bragg coherent x-ray diffractive imaging (CXDI) has been one of the dominant nondestructive imaging tools with high-resolution and refraction sensitivities that provide quantitative information in bulk and nano-scale crystals and their associated nano-devices. In this review, we will focus on the recent developments of using Bragg CXDI at the state-of-art endstations in synchrotron radiation facilities worldwide to understand the structures of ferroelectric and magnetic materials and the structural phase transition of complex materials. We will also present our perspectives on the opportunities and challenges in using Bragg CXDI techniques for materials characterization.

Overcoming Nanoscale Inhomogeneities in Thin-Film Perovskites via Exceptional Post-annealing Grain Growth for Enhanced Photodetection.

Antisolvent-assisted spin coating has been widely used for fabricating metal halide perovskite films with smooth and compact morphology. However, localized nanoscale inhomogeneities exist in these films owing to rapid crystallization, undermining their overall optoelectronic performance. Here, we show that by relaxing the requirement for film smoothness, outstanding film quality can be obtained simply through a post-annealing grain growth process without passivation agents. The morphological changes, driven by a vaporized methylammonium chloride (MACl)–dimethylformamide (DMF) solution, lead to comprehensive defect elimination. Our nanoscale characterization visualizes the local defective clusters in the as-deposited film and their elimination following treatment, which couples with the observation of emissive grain boundaries and excellent inter- and intragrain optoelectronic uniformity in the polycrystalline film. Overcoming these performance-limiting inhomogeneities results in the enhancement of the photoresponse to low-light (<0.1 mW cm–2) illumination by up to 40-fold, yielding high-performance photodiodes with superior low-light detection.

Nanoscale chemical characterization of biomolecules using tip-enhanced Raman spectroscopy.

Nanoscale chemical and structural characterization of single biomolecules and assemblies is of paramount importance for applications in biology and medicine. It aims to describe the molecular structure of biomolecules and their interaction with unprecedented spatial resolution to better comprehend underlying molecular mechanisms of biological processes involved in cell activity and diseases. Tip-enhanced Raman scattering (TERS) spectroscopy appears particularly appealing to reach these objectives. This state-of-the-art TERS technique is as versatile as it is ultrasensitive. To perform a successful TERS experiment, special care and a thorough methodology for the preparation of the TERS system, the TERS probe tip, and sample are needed. Intense efforts have been deployed to characterize nucleic acids, proteins and peptides, lipid membranes, and more complex systems such as cells and viruses using TERS. Although the vast majority of studies have first been performed in dry conditions, they have allowed for several scientific breakthroughs. These include DNA and RNA sequencing, and the determination of relationships between protein structure and biological function by the use of increasingly exploitative chemometric tools for spectral data analysis. The nanoscale determination of the secondary structure of amyloid fibrils, protofibrils and oligomers implicated in neurodegenerative diseases could, for instance, be connected with the toxicity of these species, amyloid formation pathways, and their interaction with phospholipids. Single particles of different viral strains could be distinguished from one another by comparison of their protein and lipid contents. In addition, TERS has allowed for the evermore accurate description of the molecular organization of lipid membranes. Very recent advances also demonstrated the possibility to carry out TERS in aqueous medium, which opens thrilling perspectives for the TERS technique in biological, biomedical, and potential clinical applications.

Unveiling Alternating Current Electronic Properties at Ferroelectric Domain Walls

AbstractFerroelectric domain walls exhibit a range of interesting electrical properties and are now widely recognized as functional 2D systems for the development of next‐generation nanoelectronics. A major achievement in the field is the development of a fundamental framework that explains the emergence of enhanced electronic direct‐current conduction at the domain walls. Here, the much less explored behavior of ferroelectric domain walls under applied alternating‐current (AC) voltages is discussed. An overview of the recent advances in the nanoscale characterization is provided that allows for resolving the dynamic responses of individual domain walls to AC fields. In addition, different examples are presented, showing the unusual AC electronic properties that arise at neutral and charged domain walls in the kilo‐ to gigahertz regime. It is concluded with a discussion about the future direction of the field and novel application opportunities, expanding domain‐wall‐based nanoelectronics into the realm of AC technologies.

Implementation of Nanoscale Secondary‐Ion Mass Spectrometry Analyses: Application to Ni‐Based Superalloys

Secondary‐ion mass spectrometry (SIMS) is probably the most widely used chemical analysis technique in semiconductor science and in metallurgy because of its ultimate sensitivity to all elements and in particular to light elements providing semiquantitative information on the depth distribution of elements, for instance, doping elements (i.e., B, H), contaminants (i.e., C and O), chemical gradients, and segregation in thin films and at interfaces, etc. With the size shrinking of systems, high‐resolution 3D chemical imaging is becoming a prerequisite for the development of new materials at the nanoscale and for the deep understanding of the correlation between their properties and functionalities. Herein, the development of an innovative analytical SIMS implemented in a focused ion beam (FIB) (using Ga source)/scanning electron microscope (SEM) is reported. The equipment enables to give elemental chemical mapping at very high resolution (&lt;30 nm) by precise optimization of the secondary‐ion optics and detection, while preserving excellent sensitivity, thanks to the integration of a gas injection system (GIS), which improves a positive (negative) ionization rate with oxygen (cesium) injection. The capability of the technique is demonstrated with nanoscale characterization of Ni‐based superalloys with broad applications in the manufacturing of engine parts and accessories for aircraft and aerospace equipment.

Nanoscale characterization of drug-induced microtubule filament dysfunction using super-resolution microscopy

BackgroundThe integrity of microtubule filament networks is essential for the roles in diverse cellular functions, and disruption of its structure or dynamics has been explored as a therapeutic approach to tackle diseases such as cancer. Microtubule-interacting drugs, sometimes referred to as antimitotics, are used in cancer therapy to target and disrupt microtubules. However, due to associated side effects on healthy cells, there is a need to develop safer drug regimens that still retain clinical efficacy. Currently, many questions remain open regarding the extent of effects on cellular physiology of microtubule-interacting drugs at clinically relevant and low doses. Here, we use super-resolution microscopies (single-molecule localization and optical fluctuation based) to reveal the initial microtubule dysfunctions caused by nanomolar concentrations of colcemid.ResultsWe identify previously undetected microtubule (MT) damage caused by clinically relevant doses of colcemid. Short exposure to 30–80 nM colcemid results in aberrant microtubule curvature, with a trend of increased curvature associated to increased doses, and curvatures greater than 2 rad/μm, a value associated with MT breakage. Microtubule fragmentation was detected upon treatment with ≥ 100 nM colcemid. Remarkably, lower doses (< 20 nM after 5 h) led to subtle but significant microtubule architecture remodelling characterized by increased curvature and suppression of microtubule dynamics.ConclusionsOur results support the emerging hypothesis that microtubule-interacting drugs induce non-mitotic effects in cells, and establish a multi-modal imaging assay for detecting and measuring nanoscale microtubule dysfunction. The sub-diffraction visualization of these less severe precursor perturbations compared to the established antimitotic effects of microtubule-interacting drugs offers potential for improved understanding and design of anticancer agents.

Impacts of Baobab (Adansonia digitata) Powder on the Poly(Butylene Succinate) Polymer Degradability to Form an Eco-Friendly Filler-Based Composite

Poly (butylene succinate) (PBS) is one of the most common biodegradable plastic polymers that has recently been used in the green environmental field. Enhancement of physicochemical characteristics of these polymers by using plant-based materials like Baobab (Adansonia digitata) will improve its industrial application. This study evaluated Baobab (Adansonia digitata) powder (BP) and PBS composites under various ratios (PBS/BP: 90/10, 80/20, 70/30, 60/40, and 50/50 wt%) for their thermo-mechanical and other physicochemical properties for the industrial application. The nanoscale morphological and elemental characterization were also measured by scanning electron microscope-dispersive X-ray spectroscopy (SEM-EDS). The results revealed that PBS/BP blends of 90/10 and 50/50 showed a significantly reduced melting temperature (Tm) up to 94°C (p &amp;lt; 0.05) compared to PBS (114°C). Also, the dynamic viscosity, storage modulus, and loss modulus showed a significant decrease with increasing the ratio of BP in PBS/BP composite, which confirmed faster degradation than the pure PBS. In conclusion, the novel PBS/BP biomaterial is recommended for use as a carbon source for denitrification processes, as an eco-friendly faster degradable natural filler-based polymer. Besides, they could be use in food packaging and biomedical industries.