Curcumin Nanoparticles Research Articles

Curcumin‐loaded nanoparticles effectively prevent T4‐induced oxidative stress in rat heart

AbstractIn this study, we report the cardioprotective effect of the glycerol monooleate (GMO) based nanocurcumin in both in vitro and in vivo conditions under a hyperthyroid state. The heart is one of the primary target organs sensitive to the action of thyroid hormone, and slight variations in the thyroid hormone serum concentrations result in measurable changes in cardiac performance. Hyperthyroidism‐induced hypermetabolism is associated with oxidative stress and is an important mechanism responsible for the progression of heart failure. Curcumin has been known to play a protective role against oxidative stress‐related diseases like Alzheimer's, asthma, and aging due to its antioxidant properties. Nevertheless, its potent biological activity has been hindered due to its poor bioavailability. To overcome this drawback, a GMO‐based biodegradable nanoparticle (NP) formulation loaded with curcumin has been developed, and the protective effect of curcumin‐loaded NPs was compared against the native drug. Oxidative stress parameters like reactive oxygen species (ROS) release, change in mitochondrial membrane permeability, lipid peroxidation (LPx), lactate dehydrogenase (LDH) release, and the activity and protein expression of the endogenous antioxidant enzymes like superoxide dismutase, catalase (CAT) and glutathione peroxidase were evaluated. The results from in vitro showed that curcumin‐loaded NPs showed better DPPH and NO radical scavenging activity than native curcumin in a concentrations range of 2.5–20 µM. It was also observed that the nanoparticulate curcumin was comparatively more effective than native curcumin in protecting against ROS‐induced membrane damage by reducing LPx and LDH leakage at low concentrations of 5–10 µM. Further, curcumin NPs performed better in facilitating the activities of antioxidant enzymes under in vitro and in vivo conditions with respect to time and concentrations, resulting in reduced cellular ROS levels. In this scenario, we anticipate that curcumin‐loaded NPs can serve as a better antioxidant than its native counterpart in protecting the heart from oxidative stress‐related diseases.

The effects of dietary Spirulina platensis or curcumin nanoparticles on performance, body chemical composition, blood biochemical, digestive enzyme, antioxidant and immune activities of Oreochromis niloticus fingerlings

ContextRecently, prioritize has been given to using natural phytogenic or nano compounds as growth promoters and immunostimulants in fish diets as an alternative to antibiotics.AimsThe main propose of this trial was to determine the impact of supplementing diets with spirulina or curcumin nanoparticles on the performance and health indicators of Nile tilapia fingerlings.MethodsIn a 56-day feeding trial, 180 tilapia fingerlings were assigned into three main groups, as follows: 1st, control group, 2nd, Spirulina platensis (SP; 5 g kg-1 diet) and 3rd, curcumin nanoparticles (CUR-NPs; 30 mg kg-1 diet).Key resultsIncorporating tilapia diets with SP or CUR-NPs significantly improved performance, body chemical analysis, blood biochemical and hematological indices, digestive enzyme activities, and antioxidant and immunostimulant features compared to the control.ConclusionFortified tilapia diets with CUR-NPs or SP efficiently boost the productivity and health of Nile tilapia fingerlings.ImplicationsThe research introduces new practical solutions for applying safe feed additives as alternatives to antibiotics in tilapia farming.

Curcumin-Polyethylene Glycol Loaded on Chitosan-Gelatin Nanoparticles Enhances Infected Wound Healing.

The aim of the present study was to evaluate effects of curcumin-polyethylene glycol loaded on chitosan-gelatin nanoparticles (C-PEG-CGNPs) on healing of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds in rat as a model study. Forty male Wistar rats were randomized into 5 groups of 8 animals each. In CNTRL group, no infected/no treated wounds were covered with sterile saline 0.9% solution (0.1 mL). In MRSA group, MRSA-infected wounds were only treated with sterile saline 0.9% solution (0.1 mL). In MRSA/CP group, 0.1 mL curcumin nanoparticles (1 mg/mL) was applied topically to treat MRSA-infected wounds. In MRSA/CG group, 0.1 mL CG (1 mg/mL) was applied topically to treat MRSA-infected wounds. In MRSA/CP-CG group, 0.1 mL CP-CG (1 mg/mL) was applied topically to treat MRSA-infected wounds. Microbiological examination; planimetric, biochemical, histological, morphometric studies, angiogenesis, hydroxyproline levels, and reverse transcription polymerase chain reaction for caspase 3, Bcl-2, and p53 showed significant difference between rats in MRSA/CP-CG group in comparison with other groups (P < .05). Accelerated and improved healing in wounds infected with MRSA were observed in animals treated with C-PEG-CGNPs. Via increasing solubility of curcumin in C-PEG-CGNP, this harmless and easily available composition could be considered to be topically applied in infected wounds.

Design and Development of a Polymeric-Based Curcumin Nanoparticle for Drug Delivery Enhancement and Potential Incorporation into Nerve Conduits.

Peripheral nerve injuries (PNI) impact millions of individuals in the United States, prompting thousands of nerve repair procedures annually. Nerve conduits (NC) are commonly utilized to treat nerve injuries under 3 cm but larger gaps still pose a challenge for successful peripheral nerve regeneration (PNR) and functional recovery. This is partly attributed to the absence of bioactive agents such as stem cells or growth factors in FDA-approved conduits due to safety, harvesting, and reproducibility concerns. Therefore, curcumin, a bioactive phytochemical, has emerged as a promising alternative bioactive agent due to its ability to enhance PNR and overcome said challenges. However, its hydrophobicity and rapid degradation in aqueous solutions are considerable limitations. In this work, a nanoscale delivery platform with tannic acid (TA) and polyvinylpyrrolidone (PVP) was developed to encapsulate curcumin for increased colloidal and chemical stability. The curcumin nanoparticles (CurNPs) demonstrate significantly improved stability in water, reduced degradation rates, and controlled release kinetics when compared to free curcumin. Further, cell studies show that the CurNP is biocompatible when introduced to neuronal cells (SH-SY5Y), rat Schwann cells (RSC-S16), and murine macrophages (J774 A.1) at 5 μM, 5 μM, and 10 μM of curcumin, respectively. As a result of these improved physicochemical properties, confocal fluorescence microscopy revealed superior delivery of curcumin into these cells when in the form of CurNPs compared to its free form. A hydrogen peroxide-based oxidative stress study also demonstrated the CurNP's potential to protect J774 A.1 cells against excessive oxidative stress. Overall, this study provides evidence for the suitability of CurNPs to be used as a bioactive agent in NC applications.

Epoxy resin bioactive dental implant capped with hydroxyapatite and curcumin nanoparticles: a novel approach.

In this study, the developed bioactive dental implant (BDI) from epoxy resin (ER), hydroxyapatite (HA), and curcumin nanoparticles (CUNPs). The prepared BDI were characterized using their physicochemical, mechanical, antimicrobial, bioactive, and biocompatibility study. The scanning electron microscopy (SEM) morphology of the BDI was observed HA mineralized crystal layer after being immersed in the stimulated body fluids (SBF) solution. The mechanical properties of the BDI exhibited tensile strength (250.61 ± 0.43MPa), elongation at break (215.66 ± 0.87%), flexural modulus (03.90 ± 0.12 GPa), water absorption (05.68 ± 0.15%), and water desorption (06.42 ± 0.14%). The antimicrobial activity of BDI was observed in excellent zone of inhibition against the gram-negative (15.33 ± 0.04%) and gram- positive (15.98 ± 0.07%) bacteria. The biocompatibility study of BDI on osteoblasts cell line (MG-63) was analyzed using MTT (3-[4, 5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The results were observed 85% viable cells present in the BDI compared to the control (only ER) samples. Based on the research outcome, the BDI could be used for biomaterials application, particularly tooth dental implantation.

The Effects of Adding Different Levels of Curcumin Nanoparticles and Sucrose Sugar on Qualitative and Structural Parameters of Ram Epididymal Sperm after Cooling

The Effects of Adding Different Levels of Curcumin Nanoparticles and Sucrose Sugar on Qualitative and Structural Parameters of Ram Epididymal Sperm after Cooling

Exploring the link between pyrethroids exposure and dopaminergic degeneration through morphometric, immunofluorescence, and in-silico approaches: the therapeutic role of chitosan-encapsulated curcumin nanoparticles.

Introduction: The synthetic pyrethroid derivative fenpropathrin (FNE), a commonly used insecticide, has been associated with various toxic effects in mammals, particularly neurotoxicity. The study addressed the hallmarks of the pathophysiology of Parkinson's disease upon oral exposure to fenpropathrin (FNE), mainly the alteration of dopaminergic markers, oxidative stress, and molecular docking in rat models. In addition, the protective effect of curcumin-encapsulated chitosan nanoparticles (CRM-Chs-NPs) was also assessed. Methods: In a 60-day trial, 40 male Sprague Dawley rats were divided into 4 groups: Control, CRM-Chs-NPs (curcumin-encapsulated chitosan nanoparticles), FNE (15mg/kg bw), and FNE + CRM-Chs-NPs. Results: FNE exposure induced reactive oxygen species generation, ATP production disruption, activation of inflammatory and apoptotic pathways, mitochondrial function and dynamics impairment, neurotransmitter level perturbation, and mitophagy promotion in rat brains. Molecular docking analysis revealed that FNE interacts with key binding sites of dopamine synthesis and transport proteins. On the other hand, CRM-Chs-NPs mitigated FNE's toxic effects by enhancing mitochondrial dynamics, antioxidant activity, and ATP production and promoting anti-inflammatory and antiapoptotic responses. Conclusion: In summary, FNE appears to induce dopaminergic degeneration through various mechanisms, and CRM-Chs-NPs emerged as a potential therapeutic intervention for protecting the nervous tissue microenvironment.

Curcumin nanogel and its efficacy against oxidative stress and inflammationin rat models of ischemic stroke.

Aim: The study was designed to develop and analyze curcumin nanoparticles. Methods: Curcumin nanoparticles were formulated and evaluated. Their efficacy in protecting against brain damage was investigated in a rat model of ischemic stroke, considering motor function, muscle strength and antioxidant enzyme activity. Results: Curcumin nanoparticles displayed a zeta potential of -55±13.5mV and an average particle size of 51.40±21.70nm. In ischemic stroke rat models, curcumin nanoparticletreatment significantly improved motor functions, and muscle strengthand increased theactivities of antioxidant enzymes like glutathione peroxidase, glutathione, glutathione S-transferase, superoxide dismutaseand catalase, reducing oxidative stress and inflammation. Conclusion: Curcumin nanoparticles showed significant neuroprotective effects in ischemic stroke models.

Histological and Immunohistochemical Studies on the Effect of Curcumin Nanoparticles versus Mesenchymal stem cells on Acetaminophen-Induced Acute Liver injury in Adult Male Albino Rats

Histological and Immunohistochemical Studies on the Effect of Curcumin Nanoparticles versus Mesenchymal stem cells on Acetaminophen-Induced Acute Liver injury in Adult Male Albino Rats

Investigation of Cytotoxic and Antileishmanial Activity of Hybrid Silver Nanoparticle Complexes: Potential Drug Candidates against Leishmania Species

In recent years, isolation of resistant Leishmania species to drugs in use has made it necessary to search alternative molecules that may be drug candidates. In this study, it was aimed to investigate the cytotoxic and in vitro antileishmanial activity of hybrid silver nanoparticle (AgNP) complexes. In this study, three types of nanoparticles (NPs), oxidized amylose-silver (OA-Ag) NPs, oxidized amylose-curcumin (OA-Cur) NPs and oxidized amylose-curcumin-silver (OA-CurAgNP) nanoparticles were synthesized. The cytotoxic activity of the synthesized nanoparticles was determined against L929 mouse fibroblasts and the in vitro antileishmanial activity was determined against Leishmania tropica, Leishmania infantum and Leishmania donovani isolates by the broth microdilution method. It was observed that the hybrid OA-CurAgNP complex obtained by combining curcumin and silver nanoparticles showed cytotoxic effects against L929 mouse fibroblasts at concentrations of 1074 µg/mL and above. IC50 values expressing the antileishmanial activity of the hybrid OA-CurAgNP complex against L.tropica, L.infantum and L.donovani isolates, were found to vary between 95-121 µg/mL, 202-330 µg/mL and 210-254 µg/mL, respectively. Resistance development has emerged as a major challenge in the treatment of leishmaniasis in recent times. Metallic nanoparticles are considered excellent candidates for medical applications due to their chemical and physical properties, as well as their prolonged circulation in the body. The current drugs used for leishmaniasis treatment are highly toxic, while nanoparticles offer advantages such as low toxicity and easy cellular uptake due to their nanoscale dimensions. The identification of strong efficacy in these particles may contribute scientific evidence for their potential use in leishmaniasis treatment. Therefore, the therapeutical value of OA-CurAgNP complex alone in combination with existing drugs should be examined.

Preparation and Investigation of Cellulose Acetate/Gelatin Janus Nanofiber Wound Dressings Loaded with Zinc Oxide or Curcumin for Enhanced Antimicrobial Activity.

The skin, as the largest organ, serves as a protective barrier against external stimuli. However, when the skin is injured, wound healing becomes a complex process influenced by physiological conditions, bacterial infections, and inflammation. To improve the process of wound healing, a variety of wound dressings with antibacterial qualities have been created. Electrospun nanofibers have gained significant attention in wound dressing research due to their large specific surface area and unique structure. One interesting method for creating Janus-structured nanofibers is side-by-side electrospinning. This work used side-by-side electrospinning to make cellulose acetate/gelatin Janus nanofibers. Curcumin and zinc oxide nanoparticles were added to these nanofibers. We studied Janus nanofibers' physicochemical characteristics and abilities to regulate small-molecule medication release. Janus nanofibers coated with zinc oxide nanoparticles and curcumin were also tested for antibacterial activity. The Janus nanofibers with specified physicochemical characteristics were successfully fabricated. Nanofibers released small-molecule medicines in a controlled manner. Additionally, the Janus nanofibers loaded with curcumin exhibited excellent antibacterial capabilities. This research contributes to the development of advanced wound dressings for promoting wound healing and combating bacterial infections.

Intranasal Delivery of Curcumin Nanoparticles Improves Neuroinflammation and Neurological Deficits in Mice with Intracerebral Hemorrhage.

Intracerebral hemorrhage (ICH) represents one of the most severe subtypes of stroke. Due to the complexity of the brain injury mechanisms following ICH, there are currently no effective treatments to significantly improve patient functional outcomes. Curcumin, as a potential therapeutic agent for ICH, is limited by its poor water solubility and oral bioavailability. In this study, mPEG-PCL is used to encapsulate curcumin, forming curcumin nanoparticles, and utilized the intranasal administration route to directly deliver curcumin nanoparticles from the nasal cavity to the brain. By inhibiting pro-inflammatory neuroinflammation of microglia following ICH in mice, reprogramming pro-inflammatory microglia toward an anti-inflammatory function, and consequently reducing neuronal inflammatory death and hematoma volume, this approach improved blood-brain barrier damage in ICH mice and promoted the recovery of neurological function post-stroke. This study offers a promising therapeutic strategy for ICH to mediate neuroinflammatory microenvironments.

Curcumin-loaded mesoporous polydopamine nanoparticles modified by quaternized chitosan against bacterial infection through synergistic effect

Clinically, open wounds caused by accidental trauma and surgical lesion resection are easily infected by external bacteria, hindering wound healing. Antibacterial photodynamic therapy has become a promising treatment strategy for wound infection. In this study, a novel antibacterial nanocomposite material (QMC NPs) was synthesized by curcumin, quaternized chitosan and mesoporous polydopamine nanoparticles. The results showed that 150 μg/mL QMC NPs had good biocompatibility and exerted excellent antibacterial activity against Staphylococcus aureus and Escherichia coli after blue laser irradiation (450 nm, 1 W/cm2). In vivo, QMC NPs effectively treated bacterial infection and accelerated the healing of infected wounds in mice.

Structural, emulsifying and curcumin-embedded properties of arachin nanoparticles induced by ultrasound and heat

Different types of arachin (one of the major globulins in peanut proteins) nanoparticles were prepared by ultrasound (US) and heat treatments, respectively. The results showed that short-term US treatment induced large nanoparticles (named US200 and US500, ∼267 nm) that maintained a compact globular structure; long-term US treatment induced small nanoparticles (named US500L, ∼197 nm) with a moderately unfolded structure; heat treatment induced medium-sized nanoparticles with an unfolded structure. The large nanoparticles had significantly lower surface hydrophobicity, β-sheet content, and λmax but higher enthalpy of denaturation (ΔH) than other nanoparticles. US500L and US500 exhibited the highest emulsifying activity and stability indices, respectively. As for curcumin-embedded properties, both US and heat treatments enhanced the curcumin encapsulation efficiency (from 88.53 to 94.08–97.88%) and load amount (from 1.77 to 1.88–1.96 μg/mg) of arachin. However, only curcumin in large nanoparticles was effectively protected from damage by heat and light. In simulated digestion tests, curcumin in US500L and heat-induced nanoparticles showed higher stability and bioavailability, which was mainly attributed to the slower digestion of these nanocomplexes. We have established a simple method for the preparation of arachin nanoparticles by US and the nanoparticles have potential applications in the food industry due to their favorable bifunctional properties.

Anti-gouty arthritis and anti-inflammatory effects of curcumin nanoparticles in monosodium urate crystals induced Balb/C mice.

Gout is a metabolic condition characterized by the accumulation of urate crystals in the synovial joints. These crystal depositions result in joint swelling and increased concentration of serum uric acid in blood. The commercially available drugs lower serum uric acid levels and reduce inflammation, but these standard therapies have many side effects.This study aimed to investigate anti-gout and anti-inflammatory properties of curcumin nanoparticles (CNPs). For this purpose, CNPs were prepared by dissolving curcumin into dichloromethane. Then, gout was induced by injecting monosodium urate crystals (MSU) in the ankle joint and in the intra-peritoneal cavity which caused ankle swelling and increased blood uric acid levels. CNPs in different concentrations (5, 10, and 20ppm) and allopurinol were orally administered. The MSU crystals increased the xanthine oxidase levels both in serum and the liver. Moreover, MSU crystals increased the serum levels of interleukin 1β, interleukin-6, tumor necrosis factor-alpha, liver function tests markers, renal function tests markers, and lipid profiles. However, the administration of CNPs decreased the levels of all these variables. CNPs increased the serum high-density lipoprotein and interleukin-10 levels. Moreover, CNPs also reduced ankle swelling significantly. Hence, the levels ofxanthine oxidase, uric acid and ankle swelling were reduced significantly by oral administration of CNPs. Our findingsindicate that CNPs through their anti-inflammatory properties significantly alleviate gouty arthritis. Thus, the study concluded that CNPs can be developed as an efficient anti-gout agent with minimal side effects.

Curcumin and silver nanoparticles loaded antibacterial multifunctional pectin/gelatin films for food packaging applications

Renewable biomass-based materials have a huge potential to replace petroleum-based products in food packaging. Herein, pectin/gelatin films loaded with curcumin and silver nanoparticles (AgNPs) are prepared by solution-pouring technology to serve as antimicrobial multifunctional food packaging films. AgNPs and curcumin are found to equally distribute in the films. Fourier transform infrared spectroscopy (FT-IR) reveal the hydrogen bonding and electrostatic interaction among curcumin, AgNPs, pectin and gelatin. The composite films show good antioxidant activity, mechanical performance, hydrophobicity and antibacterial ability. The films of P-GCA 0.5 showed 99.57 ± 0.16 % and 100 % inhibition against E. coli and S. aureus, respectively. The films also demonstrate excellent water vapor barrier qualities. In addition, the composite films possess pH-responsive color change behaviors from yellow (pH 3–8) to light red (pH 8–9) to dark red (pH 11–12), which is suitable for monitoring the freshness of shrimp packaging based on pH changes during deterioration process. As sustainable biomass-based materials, the multifunctional composite films are promising in intelligent food packaging applications.

Organelle-like water-borne curcumin nanoparticles with smart fluorescence for anti-counterfeiting and food quality monitoring

Current fluorescent labels are often expensive and environmentally unfriendly and tend to lose their fluorescence when combined with dry and hydrophilic substrates because of their hydrophobic nature. Thus, the development of environmentally friendly, low-cost, and scalable fluorescent materials is still highly desirable but significantly challenging, especially for anti-counterfeiting and food quality monitoring. In this study, highly fluorescent nanoparticles were prepared by mimicking organelles and using natural and sustainable curcumin as a fluorescent component. The preparation process was easily scalable via traditional seeded emulsion polymerization methods and green without using additional organic solvents. The resulting curcumin-based fluorescent nanoparticles (CFNs) were well dispersed in water, offering smart fluorescence in water-based environments or a dried polymer coating. In particular, CFNs-based coatings could be used for effective smart anti-counterfeiting and food quality monitoring. The proposed strategies may inspire the design of novel, green, and sustainable smart fluorescent materials/coatings for applications in anti-counterfeiting, smart food tags and labels, and visual sensing systems.

Microbial fermentation-based synthesis of nano-curcumin suggesting the role of pullulan in nano-formulation

Curcumin is a multitargeting nutraceutical with numerous health benefits, however, its efficacy is limited due to poor aqueous solubility and reduced bioavailability. While nano-formulation has emerged as an alternative to encounter such issues, it often involves use of toxic solvents. Microbial synthesis may be an innovative solution to address this lacuna. Present study, for the first time, reports exploitation of Aureobasidium pullulans RBF4A3 for production of nano-curcumin. For this purpose, Aureobasidium pullulans RBF4A3 was inoculated in YPD media along with curcumin (0.1 mg/mL) and incubated for 24 h, 48 h, and 72 h. Subsequently, residual sugar, biomass, EPS concentration, curcumin concentration, and curcumin nanoparticle size were measured. As a result, nano-curcumin with an average particle size of 31.63 nm and enhanced aqueous solubility was obtained after 72 h. Further, investigations suggested that pullulan, a reducing polysaccharide, played a significant role in curcumin nano-formulation. Pullulan-mediated nano-curcumin formulation, with an average particle size of 24 nm was achieved with conversion rate of around 59.19 %, suggesting improved aqueous solubility. Additionally, the anti-oxidant assay of the resulting nano-curcumin was around 53.7 % per μg. Moreover, kinetics and thermodynamic studies of pullulan-based nano-curcumin revealed that it followed first-order kinetics and was favored by elevated temperature for efficient bio-conversion. Also, various physico-chemical investigations like FT-IR, NMR, and XRD reveal that pullulan backbone remains intact while forming curcumin nanoparticle. This study may open up new avenues for synthesizing nano-polyphenols through a completely green and solvent free process with plausible diverse applications.

Fibroin Nanoparticles: Use in Drug Delivery

The silkworm Bombyx mori.L. is infected by various diseases viz; grasserie, flacherie, muscardine, and pebrine. Among all these diseases the grasserie causes major economic loss to the industry and is one of the main reasons for low silk productivity. It is caused by a virus known as Bombyx mori nucleopolyhedro virus (BmNPV). The impact of grasserie disease on silkworms is significant as it leads to reduced silk production and can result in economic losses for sericulture farmers. In India greater than 50% of silk cocoon crop loss is due to BmNPV [1] and in Kashmir valley, the loss is about 28-32% [2]. Silk obtained from the cocoons of silkworm Bombyx mori L. is a natural fibrous protein well known for being lightweight, having high mechanical strength, good flexibility, and luster making it ideal for the textile industry. In addition, fibroin extracted from the cocoons of domesticated silkworm Bombyx mori L. has gained growingly interest due to its excellent mechanical properties and high biocompatibility, biodegradability, inexpensiveness, and preparation flexibility [3]. These properties of silk fibroin lead to the formulation of fibroin nanoparticles (FNP’-s) which can be used to encapsulate different types of therapeutic compounds like proteins, vaccines, enzymes, etc. Fibroin has been approved as a biomaterial by the Food and Drug Administration (FDA) and has been popularly used in numerous medical applications such as sutures, tissue regeneration, coating devices, and drug delivery systems [4]. It has been studied that SF-derived curcumin nanoparticles show higher efficacy against breast cancer cells and have the potential to treat in vivo breast tumors by local, sustained, and long-term therapeutic delivery as a biodegradable system [5]. Therefore, fibroin-coated nanoparticles can be used effectively for disease management.

Triboelectric-Responsive Drug Delivery Hydrogel for Accelerating Infected Wound Healing.

Electrotherapy is of great interest in the field of tissue repair as an effective, well-tolerated, and noninvasive treatment. Triboelectric nanogenerator (TENG) has shown advantages in promoting wound healing due to its peak output characteristic and low Joule heating effect. However, it is limited in infected wound healing due to poor antimicrobial capacity. Here, a wearable triboelectric stimulator (WTS) is developed that consists of a flexible TENG (F-TENG) and a triboelectric-responsive drug delivery hydrogel (TR-DDH) for healing of bacterium-infected wounds. F-TENG can generate pulsed current to wounds by converting mechanical energy from body movements. Polypyrrole is prone to reduction and volume contraction under electrical stimulation, resulting in desorption of curcumin nanoparticles (CUR NPs) from the polypyrrole in TR-DDH. Therefore, the highly efficient and controllable release of CUR NPs can be achieved by triboelectric stimulation. According to the in vitro and in vivo experiments, WTS has the greatest antimicrobial effect and the fastest promotion of infected wound healing compared to treatment with electrical stimulation or curcumin. Finally, the safety assessment demonstrates that the WTS has excellent tissue safety for chronic wound healing. Synergistic therapy with WTS provides an efficient strategy for chronic wound healing and smart-responsive drug delivery systems.