Stability Of Nanoparticles Research Articles

Electron donation from carbon support enhances the activity and stability of ultrasmall ruthenium dioxide nanoparticles in acidic oxygen evolution reaction

Electron donation from carbon support enhances the activity and stability of ultrasmall ruthenium dioxide nanoparticles in acidic oxygen evolution reaction

Challenges in lignin integration within biopolymer matrices: Toward stable and effective lignin nanoparticles as additives for sustainable food packaging

Challenges in lignin integration within biopolymer matrices: Toward stable and effective lignin nanoparticles as additives for sustainable food packaging

Highly Stable Antitumor Silver-Lipid Nanoparticles Optimized for Targeted Therapy

Highly Stable Antitumor Silver-Lipid Nanoparticles Optimized for Targeted Therapy

Investigation of the morphological change of stable copper nanoparticles synthesized in different concentrations of polyvinyl alcohol and its catalytic activities on dye degradation

Investigation of the morphological change of stable copper nanoparticles synthesized in different concentrations of polyvinyl alcohol and its catalytic activities on dye degradation

A free-standing sulfide polyacrylonitrile/reduced graphene oxide film cathode with nacre-like architecture for high-performance lithium-sulfur batteries

Sulfide polyacrylonitrile (SPAN) is regarded as a promising cathode material to replace traditional carbon/sulfur composites, due to its conversion solid-solid transformation mechanism that effectively eliminates the shuttle effect of lithium sulfur batteries (LSBs). Unfortunately, its low sulfur content and slow reaction kinetics greatly affect the electrochemical performance. In this paper, a scalable production method is developed to fabricate free-standing sulfide polyacrylonitrile/reduced graphene oxide (SPAN/RGO) film cathode with nacre-like architecture. In this novel free-standing film cathode, graphene nanosheets act as a stable conductive framework and SPAN nanoparticles evenly disperse between the graphene nanosheets. The dense layered structure effectively alleviates the volume expansion of sulfur during cycling. Ex-situ Raman analysis provides evidence for the reversible cleavage and reformation of C−S/S−S bonds throughout the charge-discharge cycle. With these advantages, free-standing SPAN/RGO film cathode exhibits a low-capacity decay rate of 0.052 % over 1000 cycles at 0.5 C. Additionally, it maintains stable cycling performance even when the SPAN loading reaches 10.0 mg cm−2. This offers a straightforward and effective approach for the development of practical cathode materials for lithium-sulfur batteries (LSBs).

Rapid synthesis of manganese dioxide nanoparticles for enhanced biocompatibility and theranostic applications.

Manganese dioxide (MnO2), lauded for its biocompatibility and distinctive optical and physical characteristics, has become an indispensable material in the biomedical field, showing immense potential in disease detection, treatment, and prevention. Particularly, the ability of MnO2 nanoparticles to oxidize glutathione (GSH) to its oxidized form has positioned them as pivotal players in GSH sensing. However, conventional preparation methods, whether top-down or bottom-up, often result in nanoparticles that require multi-step processing and modification to achieve good dispersion in physiological conditions, which is both time-consuming and complex. To address this, a rapid and efficient method was developed for producing well-dispersed and stable MnO2 nanoparticles using tannic acid to reduce potassium permanganate. The polyphenolic structure of tannic acid not only facilitates the reduction process but also enhances the dispersibility of the nanoparticles in biological environments. In addition, PEG could improve the stability of MnO2 nanoparticles and also reduce their size. Moreover, we demonstrate the application of these nanoparticles in a colorimetric assay for GSH detection, leveraging their ability to react with GSH to produce Mn2+. Furthermore, these nanoparticles were utilized in a colorimetric assay for GSH detection, harnessing their reactivity with GSH to generate Mn2+. Beyond this, the MnO2 nanoparticles exhibit potential for the loading of a spectrum of molecules, including small molecules, peptides, DNA, RNA, and proteins, through electrostatic interactions, π-π stacking, and the inherent reactivity of polyphenols. This groundbreaking strategy heralds a new era for MnO2 in the realm of theranostic agent delivery, offering a promising avenue for enhancing diagnostic accuracy and therapeutic efficacy in biomedical applications.

Comparative Study of the Synthesis of Silver, Copper, and Iron oxide Nanoparticles via Chemical and Green Methods Using Corn Bract for Enhanced Antibacterial Activity

The increasing demand for sustainable and eco-friendly solutions in nanotechnology highlights the importance of exploring novel methods for the synthesis of nanoparticles. Metal nanoparticles have unique properties and are particularly significant in addressing antimicrobial resistance and environmental challenges. This study aimed to synthesize silver, copper, and iron nanoparticles from waste corn bract using chemical and green synthesis methods and evaluate their antibacterial properties. Corn bract extract was prepared and used as a reducing and stabilizing agent in green synthesis, while the comparison was done by conventional chemical methods. The nanoparticles were characterized using UV-visible spectroscopy, FTIR, and SEM, and their antibacterial activity was tested against Gram-positive (Bacillus sp., Staphylococcus sp.) and Gram-negative (Escherichia coli, Klebsiella sp., Pseudomonas sp.) bacteria through the well diffusion method. The green-synthesized silver nanoparticles exhibited the highest antibacterial efficacy, especially against Pseudomonas sp. and E. coli, followed by copper and iron nanoparticles. Phytochemicals in corn bract extract enhanced the stability and bioactivity of green-synthesized nanoparticles, as confirmed by FTIR analysis. The results of this investigation underscore the capabilities of agricultural waste in facilitating sustainable nanoparticle production, wherein green synthesis provides enhanced antibacterial attributes in comparison to traditional chemical approaches. This research emphasizes the significance of nanoparticles synthesized through green methods in addressing antimicrobial resistance and highlights their role as a scalable and environmentally sustainable option for generating effective antimicrobial agents.

QbD-Driven preparation, characterization, and pharmacokinetic investigation of daidzein-l oaded nano-cargos of hydroxyapatite

The repercussions of hormone replacement therapy (HRT) and bisphosphonates pose serious clinical challenges and warrant novel therapies for osteoporosis in menopausal women. To confront this issue, the present research aimed to design and fabricate daidzein (DZ); a phytoestrogen-loaded hydroxyapatite nanoparticles to mimic and compensate for synthetic estrogens and biomineralization. Hypothesizing this bimodal approach, hydroxyapatite nanoparticles (HAPNPs) were synthesized using the chemical-precipitation method followed by drug loading (DZHAPNPs) via sorption. The developed nanoparticles were optimized by "Design-Expert" software and underwent comprehensives in-vitro and in-vivo characterizations. The particle sizes of HAPNPs and DZHAPNPs were found to be 118.9 ± 0.15 nm and 129.3 ± 0.65 nm, respectively, consistent with their FESEM and TEM images. A notable entrapment efficiency of 87.23 ± 0.97% and drug release of 91 ± 0.85% from DZHAPNPs was observed over 90 h at pH 7.4. Moreover, the XRD and FTIR results confirmed the amorphization and compatibility of DZHAPNPs. TGA analysis indicated that the thermal stability of blank and drug-loaded nanoparticles was up to 900 °C. In an in vivo pharmacokinetic investigation, three-fold increased bioavailability of DZHAPNPs (AUC0−∞ = 7427.6 µg/mL*h) was obtained in comparison to daidzein solution (AUC0−∞ = 2299.7 µg/mL*h). The comprehensive results of the study indicate that bioceramic nanoparticles are potential carriers for DZ delivery.

Self-assembled aptamer nanoparticles for enhanced recognition and anticancer therapy through a lysosome-independent pathway.

Aptamers and aptamer-drug conjugates (ApDCs) have shown some success as targeted therapies in cancer theranostics. However, their stability in complex media and their capacity to evade lysosomal breakdown still need improvement. To address these challenges, we herein developed a one-step self-assembly strategy to improve the stability of aptamers or ApDCs, while simultaneously enhancing their delivery performance and therapeutic efficiency through a lysosome-independent pathway. This strategy involves the formation of stable complexes between disulfide monomer and aptamers (Sgc8) or ApDCs (Gem-Sgc8). Self-assembled Sgc8 NPs resisted nuclease degradation for up to 24 hours, whereas the aptamer alone degraded within just 3 hours. These self-assembled Sgc8 NPs, as well as Gem-Sgc8 NPs, demonstrated enhanced binding capabilities compared to Sgc8 aptamers or Gem-Sgc8 alone. Furthermore, lysosome-independent cellular uptake was significantly improved, which in turn increased the therapeutic efficacy of Gem-Sgc8 NPs by 2.5 times compared to Gem-Sgc8 alone. In vivo results demonstrated that Gem-Sgc8 NPs can effectively suppress the growth of tumors. The same self-assembly strategy was successfully applied to other aptamers, such as MJ5C and cMET, showing the generalizability of our method, Overall, this aptamer self-assembly strategy not only overcomes the limitations associated with instability and lysosomal degradation but also demonstrates its broad applicability, highlighting its potential as a promising avenue for advancing targeted cancer theranostics. STATEMENT OF SIGNIFICANCE: We developed a one-step self-assembly strategy to improve the stability of aptamers or ApDCs and enhance their drug therapeutic efficiency through a lysosome-independent pathway. The stability of self-assembled Sgc8 nanoparticles (NPs) was significantly improved. The resulting Sgc8 NPs or GEM-Sgc8 NPs exhibited enhanced binding ability compared to Sgc8 aptamers or GEM-Sgc8 alone, and they also facilitated lysosome-independent cellular uptake, resulting in a 2.5-fold increase in therapeutic efficacy of GEM-Sgc8-NPs. The same self-assembly strategy was successfully applied to other aptamers, such as MJ5C and cMET, showing the generalizability of our method.

Construct ZnSeTe/ZnTe Nanostructures with the Tunable Emission from 450 to 760 nm.

Developing heavy-metal-free materials with wide tunable emission is important to light-emitters. The alloying method is utilized in ZnSe magic size clusters (MSCs) with Te to form ZnSeTe and manipulate the band gap structure in ZnSe. The growth of ZnTe on alloyed ZnSeTe quantum dots (QDs) forms ZnSeTe/ZnTe core/shell nanostructures, showing the tunable photoluminescence emission peak from 450 to 760 nm with the different thicknesses of ZnTe shell. This is the record tunable range of 310 nm in the full visible and near-infrared spectra based on zinc chalcogenide nanostructures. Further coating thin ZnSe and ZnS shell on ZnSeTe/ZnTe core/shell nanostructures significantly improves the stability and quantum yield of nanoparticles. This work offers a new, cadmium-free material choice for optoelectronic devices aligning with environmental and performance requirements for modern applications.

PLGA co-loaded Salvia miltiorrhiza polysaccharide and Mn2+ as an adjuvant to induce potent immunity.

Developing a novel and potent adjuvant with excellent biocompatibility for immune response augmentation is crucial for enhancing vaccine efficacy. Here, we prepared a stable PLGA nanoparticle by encapsulating MnCl2/Salvia miltiorrhiza polysaccharide (MS-PLGA) and employed it as an adjuvant in the model antigen OVA (MS-PLGA-OVA) to elicit potent immunity. The biological experiments indicated that the MS-PLGA-OVA could effectively recruit APCs to the injection site and provoke long-term antibodies. Compared with the conventional Alum adjuvanted group, the MS-PLGA-OVA increased the IgG2a antibody titers and CD8+T cells maturation, triggering cytotoxic T lymphocyte response and inducing the activation of memory T cells. Importantly, the MS-PLGA could up-regulate the expression of TLRs and cGAS-STING pathway-related genes, thus increasing the DCs maturation, as well as the secretion of interleukin and IFN-β. Collectively, the MS-PLGA system may provide a novel and efficient adjuvant platform for various prophylactic vaccines and insights for the development of the next-generation nano adjuvant.

Iota-Carrageenan/Chitosan Nanoparticles via Coacervation: Achieving Stability for Tiny Particles

This study investigated the influence of parameters such as pH condition, polyelectrolyte concentration, polymer ratio, and order of addition of the commercial polyelectrolytes chitosan and iota-carrageenan (ι-carrageenan) on the formation of polymeric nanoparticles in suspension (coacervates). A preliminary purification step of the polymers was essential for obtaining stable nanoparticles with small sizes as impurities, particularly metal ions that interfere with complexation, are removed by dialysis. Microparticles (13.5 μm in dry diameter) are obtained when aliquots of chitosan solution are poured into the ι-carrageenan solution. In general, an excess of chitosan results in the formation of agglomerated particles. The addition of an aliquot of ι-carrageenan solution (30 mL at 0.6 mg/mL and pH 4.0) to the chitosan solution (6.0 mL at 0.3 mg/mL and pH 4.0) leads to dispersed nanoparticles with a hydrodynamic radius of 278 ± 5 nm, a zeta potential of −31 ± 3 mV, and an average dry diameter of 45 ± 11 nm. The hydrodynamic radius increases as the pH rises. The partial deprotonation of ι-carrageenan chains enhances the interaction with water molecules, causing the particles to swell. These findings contribute to the fundamental understanding of polyelectrolyte complexation processes in aqueous suspension and provide insights for developing stable nanomaterials for potential practical applications.

Mannose functionalized small molecule nanodrug self-assembled from amphiphilic prodrug connected by disulfide bonds for synergistic cancer chemotherapy and photodynamic/photothermal therapy.

Compared to conventional nanocarrier-based drug delivery technology, small-molecule-assembled nanomaterials provide various advantages, including higher drug loading efficiency, lower excipient-related toxicity, and a simpler formulation process. Our research constructed a mannonse-modified small-molecule-assembled nanodrug for synergistic photodynamic/chemotherapy against A549 cancer cells. The hydrophobic hypoxic-activated agent tirapazamine (TPZ) and a hydrophilic fluorescence probe Cyanine 3 (Cy3) constitute this amphiphilic prodrug via a glutathione (GSH)-responsive linkage, which could self-assemble into stable nanoparticles (NPs) and encapsulate a newly synthesized photosensitizer (SeBDP). To enhance the tumor targeting capability, we introduced a tumor-targeted nanodrug SeBDP@TPZ-S-S-Cy/Man NPs by co-assembling mannose-modified lipid (DSPE-PEG-Man). The GSH-responsive linkage of TPZ-S-S-Cy can be rapidly cleaved by GSH to release the therapeutic agents and fluorescent molecule. The released SeBDP generate reactive oxygen species (ROS) to specifically kill cancer cells and elevate hypoxia, thereby enhancing the cytotoxicity of TPZ. SeBDP@TPZ-S-S-Cy/Man NPs exhibited high selectivity and efficiency for in vivo combination therapy without adverse effects to normal tissues. Our findings demonstrate that SeBDP@TPZ-S-S-Cy/Man NPs have great potential for enhancing cancer treatment both in vitro and in vivo by combining an oxygen depletion prodrug with a hypoxia-activated antitumor agent. Thus, the GSH-sensitive self-assembled nanodrug from an amphiphilic hypoxia-activated prodrug, could serve as a potential drug carrier in targeted synergistic cancer therapy.

A Comprehensive Review of Solid Lipid Nanoparticles

Liposomes, polymeric nanoparticles, and emulsions are alternatives to other popular colloidal carriers. Because of its advantages, solid lipid nanoparticles were developed in the early 1990s, including controlled drug release, focused drug delivery, and outstanding durability. The many methods utilized to manufacture solid lipid nanoparticles and excipients, including the membrane contractor technique, are summarised in this article, along with their possible benefits and drawbacks. Solid lipid nanoparticle (SLN) stability relies on maintaining particle size, drug encapsulation, and integrity over time. Excipients like surfactants and lipids influence stability, preventing aggregation and oxidation. Drying techniques, such as spray drying and lyophilization, enhance stability by converting SLNs to solid forms, while lipid composition and drug-lipid compatibility are crucial factors. So, the instrumental techniques employed and the difficulties associated with SLN manufacturing are thoroughly examined. Particular focus is placed on the SLN release pattern and drug integration models in SLN. The main uses of SLNs, including targeted drug delivery, and the analytical approaches used in SLN assessments, are covered in detail. The main objective of this work is a thorough overview of solid lipid nanoparticles, including production methods, characterization, and routes of administration. A discussion of the components of the SLN delivery mechanism and the in vivo destiny of the carriers are also included.

Physicochemical and rheological characterization of plant-based proteins, pectin, and chitin nanofibers for developing high internal phase Pickering emulsions as potential fat alternatives.

This study evaluated the properties of lentil protein, pea protein, quinoa protein, and soy protein as natural nanoparticle stabilizers and their interactions with pectin and chitin nanofiber in preparing high internal phase Pickering emulsions (HIPPEs). The globular plant proteins interact with polysaccharides through hydrogen bonding and electrostatic interactions, transforming the structure into complex morphologies, including fibrous and elliptical shapes. These complex nanoparticles exhibited enhanced thermal decomposition stability, and the HIPPEs constructed by them demonstrated significantly improved apparent viscosity and elastic modulus, with a yield stress of 931.9Pa, showing gel-like viscoelastic characteristics. The complex system not only reduced droplet size but also formed a compact network structure, which enabled the emulsion to maintain excellent stability under heat treatment, long-term storage and high-speed centrifugation. Our findings revealed the promising potential of utilizing plant-based proteins with polysaccharides to prepare HIPPEs for developing fat alternatives.

The Stability of Cathode Catalyst Layer With Platinum‐Based Catalysts in Proton Exchange Membrane Fuel Cells

AbstractProton exchange membrane fuel cells (PEMFCs) have attracted significant interest as one of the most promising green energy technologies. However, the stability of platinum‐based catalyst layer at the cathode hinders the large‐scale application of the PEMFCs. This review systematically summarized the latest research progress on the stability of catalyst layer from the stability of nanoparticles, corrosion resistance of carbon support, chemical stability of ionomer and weakened toxic effect of ionomer on platinum particles. Strategies to enhance the stability of the catalyst layer were discussed as well, including increasing the dissolution potential of the nanoparticles, enhancing the metal–support interaction, modifying the structure and composition of the carbon support, and improving the distribution of the ionomer. This review aims to provide a comprehensive overview of the stability of cathode catalyst layer, serving as a reference for the design of catalyst layer with stability in the future.

Poly Lactic Co-glycolic Acid d-α-tocopheryl Polyethylene Glycol 1000 Succinate Fabricated Polyethylene Glycol Hybrid Nanoparticles of Imatinib Mesylate for the Treatment of Glioblastoma Multiforme.

This study aimed to develop Imatinib Mesylate (IMT)-loaded Poly Lactic-co-Glycolic Acid (PLGA)-D-α-tocopheryl polyethylene glycol succinate (TPGS)- Polyethylene glycol (PEG) hybrid nanoparticles (CSLHNPs) with optimized physicochemical properties for targeted delivery to glioblastoma multiforme. Glioblastoma multiforme (GBM) is the most destructive type of brain tumor with several complications. Currently, most treatments for drug delivery for this disease face challenges due to the poor blood-brain barrier (BBB) and lack of site-specific delivery. Imatinib Mesylate (IMT) is one of the most effective drugs for GBM, but its primary issue is low bioavailability. Therefore, nanotechnology presents a promising solution for targeted IMT delivery to GBM. This article primarily explores the fabrication of IMT-loaded core-shell lipid-polymer hybrid nanoparticles (CSLHNPs) to achieve enhanced brain delivery with therapeutic efficacy. The primary objective of this study is to develop optimized, stable IMT-loaded hybrid nanoparticles with an encapsulated polymer matrix and to evaluate these nanoparticles using sophisticated instruments such as SEM and TEM to achieve smooth, spherical nanoparticles in a monodispersed phase. The enhanced stable formulation yielded a notable increase in entrapment efficiency, reaching 58.89 ± 0.5%. The physical stability analysis of nanoparticles was assessed over 30 days under conditions of 25 ± 2°C and 60 ± 5% relative humidity. Hemolytic assays affirmed the biocompatibility and safety profile of the nanoparticles. in vitro drug release kinetics revealed a sustained IMT release over 48 hours. The formulated CSLHNPs achieved a narrow size distribution with a mean vesicle diameter of 155.03 ± 2.41 nm and a low polydispersity index (PDI) of 0.23 ± 0.4, indicating monodispersity. A high negative zeta potential of -23.89 ± 3.47 mV ensured excellent colloidal stability in physiological conditions. XRD analysis confirmed the successful encapsulation of IMT within the nanoparticle matrix, with the drug transitioning to an amorphous state for enhanced dissolution. During Cell-Cell viability assays on LN229, glioblastoma cells were treated with IMT-loaded nanoparticles and showed a significantly enhanced inhibitory effect compared to free IMT. These hybrid nanoparticles demonstrated potential in reducing oxidative stress-induced cellular damage by mitigating reactive oxygen species (ROS). Thus, the prepared IMT hybrid nanoparticles showed higher cellular uptake and superior cytotoxicity compared to the plain drug. This study posits the IMT-PLGA-TPGS-DSPE PEG 2000-CSPLHNPs as a formidable and innovative drug delivery system for Glioblastoma Multiforme (GBM) treatment, warranting further exploration into their clinical application potential. Future work could involve conducting in vivo studies to evaluate the pharmacokinetics, biodistribution, and therapeutic efficacy of the IMT-PLGA-TPGS-DSPE PEG 2000-CSPLHNPs in animal models of Glioblastoma Multiforme (GBM). Additionally, further research may focus on optimizing the nanoparticle formulation for enhanced targeting capabilities, investigating long-term stability under varied storage conditions, exploring potential combination therapies to synergize with the nanoparticles, and assessing the scalability and manufacturability of the developed drug delivery system for potential clinical translation. Integration of advanced imaging techniques for real- time tracking and visualization of nanoparticle distribution within tumours could also be a promising direction for future investigations.

Genetic determinants of silver nanoparticle resistance and the impact of gamma irradiation on nanoparticle stability

BackgroundOne of the main issues facing public health with microbial infections is antibiotic resistance. Nanoparticles (NPs) are among the best alternatives to overcome this issue. Silver nanoparticle (AgNPs) preparations are widely applied to treat multidrug-resistant pathogens. Therefore, there is an urgent need for greater knowledge regarding the effects of improper and excessive use of these medications. The current study describes the consequences of long-term exposure to sub-lethal concentrations of AgNPs on the bacterial sensitivity to NPs and the reflection of this change on the bacterial genome.ResultsChemical methods have been used to prepare AgNPs and gamma irradiation has been utilized to produce more stable AgNPs. Different techniques were used to characterize and identify the prepared AgNPs including UV-visible spectrophotometer, Fourier Transform Infrared (FT-IR), Dynamic light scattering (DLS), and zeta potential. Transmission electron microscope (TEM) and Scanning electron microscope (SEM) showed 50–100 nm spherical-shaped AgNPs. Eleven gram-negative and gram-positive bacterial isolates were collected from different wound infections. The minimum inhibitory concentrations (MICs) of AgNPs against the tested isolates were evaluated using the agar dilution method. This was followed by the induction of bacterial resistance to AgNPs using increasing concentrations of AgNPs. All isolates changed their susceptibility level to become resistant to high concentrations of AgNPs upon recultivation at increasing concentrations of AgNPs. Whole genome sequencing (WGS) was performed on selected susceptible isolates of gram-positive Staphylococcus lentus (St.L.1), gram-negative Klebsiella pneumonia (KP.1), and their resistant isolates St.L_R.Ag and KP_R.Ag to detect the genomic changes and mutations.ConclusionsFor the detection of single-nucleotide polymorphisms (SNPs) and the identification of all variants (SNPs, insertions, and deletions) in our isolates, the Variation Analysis Service tool available in the Bacterial and Viral Bioinformatics Resource Center (BV-BRC) was used. Compared to the susceptible isolates, the AgNPs-resistant isolates St.L_R.Ag and KP_R.Ag had unique mutations in specific efflux pump systems, stress response, outer membrane proteins, and permeases. These findings might help to explain how single-nucleotide variants contribute to AgNPs resistance. Consequently, strict regulations and rules regarding the use and disposal of nano waste worldwide, strict knowledge of microbe-nanoparticle interaction, and the regulated disposal of NPs are required to prevent pathogens from developing nanoparticle resistance.

Control of Nanoparticle Size of Intrinsically Fluorescent PET (Polyethylene Terephthalate) Particles Produced Through Nanoprecipitation.

Plastics are widely produced due to their stability and ease of manufacturing, but many of them quickly become a waste, breaking down into microplastics and nanoplastics. While methods for the identification and characterization of plastic particles are well consolidated, the small size of nanoplastics presents challenges for their detection and analysis. Furthermore, due to the difficulty of identifying nanoplastics, analytical studies concerning their effect on cells and a comprehensive spectroscopic characterization are still lacking. In this paper, we overcome this obstacle by synthesizing and characterizing, for the first time, PET nanoparticles with specific, stable dimensions through a top-down approach. Using hexafluoroisopropanol-chloroform as a solvent, we prepared PET solutions at various concentrations and analyzed their spectral properties over time. Our results show that PET aggregates into nanoparticles, the quantity of which increases with concentration. These findings provide crucial insights for the detection of nanoplastics in environmental samples through fluorescence measurements and can potentially be used to produce stable PET nanoparticles to evaluate their cytotoxicity.

Lipid nanoparticles deliver DNA-encoded biologics and induce potent protective immunity

Lipid nanoparticles (LNPs) for mRNA delivery have advanced significantly, but LNP-mediated DNA delivery still faces clinical challenges. This study compared various LNP formulations for delivering DNA-encoded biologics, assessing their expression efficacy and the protective immunity generated by LNP-encapsulated DNA in different models. The LNP formulation used in Moderna’s Spikevax mRNA vaccine (LNP-M) demonstrated a stable nanoparticle structure, high expression efficiency, and low toxicity. Notably, a DNA vaccine encoding the spike protein, delivered via LNP-M, induced stronger antigen-specific antibody and T cell immune responses compared to electroporation. Single-cell RNA sequencing (scRNA-seq) analysis revealed that the LNP-M/pSpike vaccine enhanced CD80 activation signaling in CD8+ T cells, NK cells, macrophages, and DCs, while reducing the immunosuppressive signals. The enrichment of TCR and BCR by LNP-M/pSpike suggested an increase in immune response specificity and diversity. Additionally, LNP-M effectively delivered DNA-encoded antigens, such as mouse PD-L1 and p53R172H, or monoclonal antibodies targeting mouse PD1 and human p53R282W. This approach inhibited tumor growth or metastasis in several mouse models. The long-term anti-tumor effects of LNP-M-delivered anti-p53R282W antibody relied on memory CD8+ T cell responses and enhanced MHC-I signaling from APCs to CD8+ T cells. These results highlight LNP-M as a promising and effective platform for delivering DNA-based vaccines and cancer immunotherapies.