Curcumin Nanoparticles Research Articles

Neuroprotective Effects of Nano-Curcumin against Cypermethrin Associated Oxidative Stress and Up-Regulation of Apoptotic and Inflammatory Gene Expression in Rat Brains.

Cypermethrin (CPM) is the most toxic synthetic pyrethroid that has established neurotoxicity through oxidative stress and neurochemical agitation in experimental rats. The toxic effects are supposed to be mediated by modifying the sodium channels, reducing Na-K ATPase, acetylcholine esterase (AchE), and monoamine oxidase (MAO). The use of curcumin nanoparticles (NC) that have potent antioxidant, anti-inflammatory and antiapoptotic properties with improved bioavailability attenuates neurotoxicity in rat brains. To test this hypothesis, animals were divided into five groups, each having six animals. Group-I control received vehicle only, while Group-II was treated with 50 mg/kg CPM. Group-III and Group-IV received both CPM and NC 2.5 mg/kg and 5 mg/kg, respectively. Group-V received 5 mg of NC alone. The CPM and NC were given by oral route. Afterwards, brain antioxidant status was measured by assessing lipid peroxidation (LPO), 4-HNE, glutathione reduced (GSH), antioxidant enzyme catalase, and superoxide dismutase (SOD) along with neurotoxicity markers Na-K ATPase, AchE, and MAO. Inflammation and apoptosis indices were estimated by ELISA, qRT-PCR, and immunohistochemistry, while morphologic changes were examined by histopathology. Observations from the study confirmed CPM-induced neurotoxicity by altering Na-K ATPase, AchE, and MAO, and by decreasing the activity of antioxidant enzymes and GSH. Oxidative stress marker LPO and the level of inflammatory interleukins IL-6, IL-1β, and TNF-α were notably high, and elevated expressions of Bax, NF-kB, and caspase-3 and -9 were reported in CPM group. However, NC treatment against CPM offers protection by improving antioxidant status and lowering LPO, inflammation, and apoptosis. The neurotoxicity marker's enzyme successfully attenuated after NC treatment. Therefore, this study supports the administration of NC effectively ameliorated CPM-induced neurotoxicity in experimental rats.

Cerium Oxide Nanoparticles: Synthesis and Characterization Using Curcuma longa (Turmeric Rhizome)

Cerium oxide and turmeric are widely used in the medical field because of their distinctive properties. Turmeric, predominantly used in Asian cuisines, is also an anti-inflammatory element in ayurvedic medicines. The lower bioavailability of turmeric rhizomes has restricted their medical applications to a specific limit, both in vitro and in vivo. Previous investigations have reported that the conjugation of curcumin and cerium oxide nanoparticles improves turmeric’s stability, making them efficient in treating chronic diseases such as cancer. The main objective of this study is to carry out the synthesis of cerium oxide nanoparticles using turmeric rhizomes. Various methods were used to characterize the cerium oxide nanoparticles. Scanning electron microscopy, energy dispersive X-ray spectroscopy, and field emission scanning electron microscopy were used to determine the morphological properties of the cerium oxide nanoparticles. Fourier transform infrared spectroscopy and X-ray diffraction were used to determine the structural properties of those nanoparticles. The BEAS-2B cells were cultured in a controlled environment, and the biocompatibility studies were conducted using an MTT assay to assess their cytotoxicity. Based on toxicity studies of cerium oxide nanoparticles, future research will be performed on their biomedical applications.

Bioavailability of nanoemulsions modified with curcumin and cerium dioxide nanoparticles

Bioavailability of nanoemulsions modified with curcumin and cerium dioxide nanoparticles

Pathophysiological basis and promise of experimental therapies for Gulf War Illness, a chronic neuropsychiatric syndrome in veterans.

This article describes the pathophysiology and potential treatments for Gulf War Illness (GWI), which is a chronic neuropsychiatric illness linked to a combination of chemical exposures experienced by service personnel during the first Gulf War in 1991. However, there is currently no effective treatment for veterans with GWI. The article focuses on the current status and efficacy of existing therapeutic interventions in preclinical models of GWI, as well as potential perspectives of promising therapies. GWI stems from changes in brain and peripheral systems in veterans, leading to neurocognitive deficits, as well as physiological and psychological effects resulting from multifaceted changes such as neuroinflammation, oxidative stress, and neuronal damage. Aging not only renders veterans more susceptible to GWI symptoms, but also attenuates their immune capabilities and response to therapies. A variety of experimental models are being used to investigate the pathophysiology and develop therapies that have the ability to alleviate devastating symptoms. Over two dozen therapeutic interventions targeting neuroinflammation, mitochondrial dysfunction, neuronal injury, and neurogenesis are being tested, including agents such as curcumin, curcumin nanoparticles, monosodium luminol, melatonin, resveratrol, fluoxetine, rolipram, oleoylethanolamide, ketamine, levetiracetam, nicotinamide riboside, minocycline, pyridazine derivatives, and neurosteroids. Preclinical outcomes show that some agents have promise, including curcumin, resveratrol, and ketamine, which are being tested in clinical trials in GWI veterans. Neuroprotectants and other compounds such as monosodium luminol, melatonin, levetiracetam, oleoylethanolamide, and nicotinamide riboside appear promising for future clinical trials. Neurosteroids have been shown to have neuroprotective and disease-modifying properties, which makes them a promising medicine for GWI. Therefore, accelerated clinical studies are urgently needed to evaluate and launch an effective therapy for veterans displaying GWI.

Improving antimicrobial activity against endodontic biofilm after exposure to blue light-activated novel curcumin nanoparticle

New therapies involving natural products and nanobiotechnology open additional perspectives to reduce endodontic infections. Curcumin is a natural polyphenol extracted from the dry rhizome of curcuma long Linn with therapeutic properties for application in nanobiotechnology and as a photosensitizer for photodynamic therapy. This study aimed to synthesize a novel polymeric nanoparticle of poly (lactic-co-glycolic acid) (PLGA) loaded with curcumin (NP+Cur), and evaluate its antimicrobial activity against endodontic biofilms. Additionally, its biocompatibility using oral keratinocytes was assessed. The polymeric NP+Cur was prepared by the nanoprecipitation method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were calculated for the three endodontic bacteria (Enterococcus faecalis, Streptococcus oralis and Actinomyces viscosus). Antibacterial activity of NP+Cur against single- and multispecies biofilm pre-formed on the botton 24-well plate and into dentin tubules of bovine teeth were evaluated by colony forming units and confocal laser scanning microscopy. The pre-irradiation time was 5 min followed by exposure to blue light-emitting diode at 450 nm for the photodynamic treatment. Cell viability using oral keratinocytes was assessed by Alamar Blue assay. MIC and MBC showed antibacterial activity of NP+Cur against endodontic bacteria. A treatment of pre-formed biofilms of endodontic bacteria with NP+Cur also significantly decreased bacterial viability. The concentration of 325 μg/mL of photoactivated NP+Cur was the one that most reduced the viability of the endodontic bacteria evaluated. Regarding biocompatibility, NP+Cur 325 μg/mL and pure nanoparticles showed a cell viability greater than 80%. The novel polymeric nanoparticles loaded with curcumin may be a promising adjunct use to treatment of endodontic infections.

Phytofabrication and Characterisation of Zinc Oxide Nanoparticles Using Pure Curcumin.

Zinc oxide and curcumin, on their own and in combination, have the potential as alternatives to conventional anticancer drugs. In this work, zinc oxide nanoparticles (ZnO NPs) were prepared by an eco-friendly method using pure curcumin, and their physicochemical properties were characterised. ATR-FTIR spectra confirmed the role of curcumin in synthesising zinc oxide curcumin nanoparticles (Green-ZnO-NPs). These nanoparticles exhibited a hexagonal wurtzite structure with a size and zeta potential of 27.61 ± 5.18 nm and -16.90 ± 0.26 mV, respectively. Green-ZnO-NPs showed good activity towards studied bacterial strains, including Escherichia coli, Staphylococcus aureus and methicillin-resistant Staphylococcus aureus. The minimum inhibitory concentration of Green-ZnO-NPs was consistently larger than that of chemically synthesised ZnO NPs (Std-ZnO-NPs) or mere curcumin, advocating an additive effect between the zinc oxide and curcumin. Green-ZnO-NPs demonstrated an efficient inhibitory effect towards MCF-7 cells with IC50 (20.53 ± 5.12 μg/mL) that was significantly lower compared to that of Std-ZnO-NPs (27.08 ± 0.91 μg/mL) after 48 h of treatment. When Green-ZnO-NPs were tested against Artemia larvae, a minimised cytotoxic effect was observed, with LC50 being almost three times lower compared to that of Std-ZnO-NPs (11.96 ± 1.89 μg/mL and 34.60 ± 9.45 μg/mL, respectively). This demonstrates that Green-ZnO-NPs can be a potent, additively enhanced combination delivery/therapeutic agent with the potential for anticancer therapy.

Light-Activated Chemically Reactive Fibrous Patch Revolutionizes Wound Repair Through the Prevention of Postoperative Adhesion.

A light-activated chemically reactive fibrous patch (ChemPatch) with tissue adhesion and wound healing activity was developed for preventing postoperative peritoneal adhesion. ChemPatch was constructed by an integrative electrospinning fabrication strategy, generating multifunctional PCL-NHS fibers encapsulating antioxidant curcumin and MnO2 nanoparticles. ChemPatch exhibited excellent photothermal conversion, which not only reformed the physical state to match the tissue but also improved conjugation between ChemPatch and tissues, allowing for strong attachment. Importantly, ChemPatch possessed good antioxidant and radical scavenging activity, which protected cells in an oxidative microenvironment and improved tissue regeneration. Particularly, ChemPatch acted as a multifunctional barrier and could not only promote reepithelialization and revascularization in wound defect model but simultaneously ameliorate inflammation and prevent postoperative peritoneal adhesion in a mouse cecal defect model. Thus, ChemPatch represents a dual-active bioadhesive barrier for reducing the incidence and severity of peritoneal adhesions.

Emulsion electrospraying and spray drying of whey protein nano and microparticles with curcumin

This study describes the potential of nanoencapsulation of curcumin using the oil-in-water emulsion electrospraying technique. Whey protein was used as wall material, coconut oil was substituted as carrier material for curcumin and the emulsion was prepared at 1:200 and 1:500 core-to-wall (curcumin: whey protein) ratios through high-speed homogenization. Encapsulated micro and nanoparticles were produced by spray drying and electrospraying techniques, respectively, and the influence of both encapsulation processes and core-to-wall ratios on the physicochemical and functional stability of encapsulated curcumin was studied. At the 1:500 core-to-wall ratio, the resulting electrosprayed particles showed a smooth spherical shape with size in the nanoscale range (∼371 nm). Electrosprayed particles with a 1:500 core-to-wall ratio exhibited higher encapsulation efficiency with ∼88% retention of curcumin, around 1.08-fold higher than spray dried particles. Fourier transform infrared spectroscopy study explained the interactions of whey protein with coconut oil containing curcumin through hydrogen bonding and hydrophobic interactions. Interactions had a positive impact on the stability of encapsulated curcumin during simulated gastric and intestinal conditions. Solubility of the curcumin was enhanced in all encapsulated particles as observed through dissolution studies; in particular, electrosprayed particles showed higher dissolution behavior as compared to spray dried particles. Electrosprayed curcumin nanoparticles with a 1:500 core-to-wall ratio showed significant protection against degradation of curcumin under simulated gastric and intestinal conditions and had higher bioaccessibility (∼83%) than other formulations. Thus, the proposed study explains a promising strategy for the production of nanoencapsulated particles with enhanced stability of curcumin, and the results of this work can be extended to functional food applications.

Stimulus-responsive curcumin-based polydopamine nanoparticles for targeting Parkinson’s disease by modulating α-synuclein aggregation and reactive oxygen species

Parkinson’s disease (PD) is the second most common neurodegenerative disease, posing a significant threat to an individual’s health, particularly among the elderly. An incomplete understanding of the pathogenesis underlying PD renders the single therapeutic model ineffective. Oxidative stress and abnormal alpha-synuclein (α-Syn) aggregation are two crucial factors accounting for dopaminergic neuron loss. Therefore, combined PD therapy that inhibits and reduces α-Syn aggregation and regulates the balance of reactive oxygen species (ROS) holds promise. In this study a novel nanodrug, polydopamine-based curcumin-loaded nanoparticles (PC NPs), based on polydopamine (PDA)-assembled curcumin (Cur) nanoparticles was designed, which was then modified with a peptide obtained from rabies virus glycoprotein (RVG) 29 (obtained RPC NPs) to target the brain. Cur and PDA of ROS-responsive nanoparticles (RPC NPs) significantly scavenged a broad spectrum of ROS. Cur inhibited and removed α-Syn aggregation, thus protecting PC12 cells from rotenone-induced cell death. RPC NPs were also shown to prolong the lifespan of Caenorhabditis elegans and restored dopamine-dependent behaviors, including area-restricted searching, ethanol avoidance, and the basal slowing response, by using 6-hydroxydopamine (6-OHDA)-pretreated C. elegans wild-type Bristol (N2) and the nematode transgenic hus111 strain as models. In vivo animal studies showed that RPC NPs significantly lowered dopaminergic neuron damage and improved the neurobehavioral abnormalities in PD mice, while having no noticeable systemic side effects. RPC NPs also decreased α-Syn accumulation and reduced oxidative stress in the striatum and substantia nigra. This multifunctional synergetic strategy may provide a new candidate for highly efficient combination therapy in patients with PD.

Fabrication, Evaluation, and Antioxidant Properties of Carrier-Free Curcumin Nanoparticles.

Curcumin (Cur), a natural hydrophobic polyphenolic compound, exhibits multiple beneficial biological activities. However, low water solubility and relative instability hinder its application in food fields. In this study, carrier-free curcumin nanoparticles (CFC NPs) were prepared by adding the DMSO solution of Cur into DI water under continuous rapid stirring. The morphology of CFC NPs was a spherical shape with a diameter of 65.25 ± 2.09 nm (PDI = 0.229 ± 0.107), and the loading capacity (LC) of CFC NPs was as high as 96.68 ± 0.03%. The thermal property and crystallinity of CFC NPs were investigated by XRD. Furthermore, the CFC NPs significantly accelerated the release of Cur in vitro owing to its improved water dispersibility. Importantly, CFC NPs displayed significantly improved DPPH radical scavenging activity. Overall, all these results suggested that CFC NPs would be a promising vehicle to widen the applications of Cur in food fields.

Curcumin nanoparticles impregnated collagen/demineralized bone matrix/olive leaves extract biocomposites as a potential bone implant: Preparation, characterization, and biocompatibility

Curcumin nanoparticles impregnated collagen/demineralized bone matrix/olive leaves extract biocomposites as a potential bone implant: Preparation, characterization, and biocompatibility

An effective biodegradable curcumin loaded magnetic microsphere: Applications for drug delivery and cancer treatment

IntroductionThe non-specific delivery of drugs to tissues is one of the major problems in traditional chemotherapy that creates problems, importantly cytotoxicity to healthy cells. A drug carrier can be designed and synthesized using a variety of techniques that allow the drug to be delivered to a specific target site with sustained release. Here, we designed novel polymer-encapsulated magnetic microspheres as drug carriers that enable site-specific and controlled drug delivery to improve therapeutic outcomes. In our study, we developed a novel emulsion-based technique to produce curcumin-loaded polymer-encapsulated magnetic microspheres. MethodsThe method involves the preparation of a binary emulsion system (water-in-oil-in-water (W1-O-W2) followed by evaporation of the continuous aqueous phase to produce magnetic polylactic acid (PLA) microspheres containing curcumin (CUR) and ferrite magnetic nanoparticles (FA-MMS). The construction of the prepared Ferrite@FA-CUR-PLA-MMS drug carrier was characterized using various spectroscopic and microscopic techniques. ResultsThe prepared Ferrite@FA-CUR-PLA-MMS drug displayed a unique and constant pH-dependent curcumin release from the drug carriers. The cytotoxicity determination of the Ferrite@FA-CUR-PLA-MMS drug by MTT assays confirmed a low cytotoxic effect on A549 and Hela cancer cells with a minimum inhibitory concentration (IC50) for A549 cells at 15 µg/ml for 24 h and for Hela cells, the IC50 value was 12 µg/ml. After 24 h, the IC50 values for A549 and Hela cancer cells were found to be 10.2 µg/ml and 8.8 µg/ml, respectively. After 10 days of incubation, the drug exhibited total destruction of the A549 and Hela cancer cells. DiscussionOur findings have greater implications for the potential use of magnetic nanoparticles in cancer chemotherapy. Further investigations on the application of this drug delivery system could be extended to the site-specific and controlled delivery of other cancer chemotherapeutic agents.

Expression of Concern: Nanoparticle curcumin ameliorates experimental colitis via modulation of gut microbiota and induction of regulatory T cells.

Expression of Concern: Nanoparticle curcumin ameliorates experimental colitis via modulation of gut microbiota and induction of regulatory T cells.

Effect of nano-curcumin on various diseases: A comprehensive review of clinical trials.

The antioxidant, anti-inflammatory, and antibacterial properties of curcumin have made it a valuable herbal product for improving various disorders, such as COVID-19, cancer, depression, anxiety, osteoarthritis, migraine, and diabetes. Recent research has demonstrated that encapsulating curcumin in nanoparticles might improve its therapeutic effects and bioavailability. To our knowledge, the efficacy of nano-curcumin on different aspects of health and disease has not been summarized in a study. Therefore, this review aimed to evaluate nano-curcumin's efficacy in various diseases based on the findings of clinical trials. In order to review publications focusing on nanocurcumin's impact on various diseases, four databases were searched, including PubMed, Scopus, Web of Science, and Google Scholar. This review highlights the potential benefits of nano-curcumin in improving a wide range of human diseases including COVID-19, neurological disorders, chronic disease, oral diseases, osteoarthritis, metabolic syndrome, and other diseases, especially as an adjunct to standard therapy and a healthy lifestyle.

Evaluation of Curcumin Nanoparticles against Rabbit Skin Infection

Curcumin is a polyphenolic compound with multiple beneficial effects in human health. It has shown a remarkable anti-inflammatory, antibacterial, antifungal, anticancer, and antioxidant effects. The limitations of curcumin are primarily in its bioavailability. Curcumin nanoparticles is suggested to reduce the limitation of bulk curcumin and probably enhance its desired effect. Curcumin was turned into nanoparticles by using simple wet-milling method. The characterization of curcumin nanoparticles indicated the presence of curcumin in size less than 100 nm. The effect of both curcumin and curcumin nanoparticles on wound healing was investigated in rabbits, in paraffin wax. Rabbits whom under curcumin and curcumin nanoparticles have shown faster healing from day 5 compared to control rabbits whom showed a proximate advancement after day 7. On day 14, rabbits whom treated with bulk and nanosized curcumin have shown a perfect healing with no sign of infection. Furthermore, both bulk and nanosized curcumin have shown a comparable antioxidant effect with ascorbic acid. Furthermore, curcumin nanoparticles were exhibited a slightly powerful antioxidant effect compared to curcumin.

Curcumin nanoparticles: physicochemical fabrication, characterization, antioxidant, enzyme inhibition, molecular docking and simulation studies.

Curcumin is an extensively studied natural compound due to its extensive biological applications. However, there are some drawbacks linked to this compound such as poor absorption, low water-solubility, quick systemic elimination, fast metabolism, poor pharmacokinetics, low bioavailability, low penetration targeting efficacy and low stability. To overcome these drawbacks, curcumin is encapsulated in nano-carriers. In the current studies, we synthesized nanoparticles of curcumin without using nanocarriers by different methods such as nano-suspension (Cur-NSM), sonication (Cur-SM) and anti-solvent precipitation (Cur-ASP) to enhance the solubility of curcumin in water. The prepared nanoparticles were characterized by FTIR, SEM and XRD analysis. These curcumin nanoparticles were screened for their solubilities in water, DPPH scavenging, amylase, α-glucosidase and β-glucosidase enzymatic activities. The particle size of nano-curcumin was found to be in the 47.4-98.7 nm range. The reduction in particle size of curcumin dramatically increases its solubility in water to 79.2 μg mL-1, whereas the solubility of curcumin is just 0.98 μg mL-1. Cur-ASP showed the highest free radical scavenging potential (48.84 ± 0.98%) which was comparable with standard BHT (50.48 ± 1.11%) at 75.0 μg mL-1. As well, Cur-ASP showed the highest inhibition of α-amylase (68.67 ± 1.02%), α-glucosidase (58.30 ± 0.52%), and β-glucosidase (64.80 ± 0.43%) at 100 μg mL-1 which is comparable with standard drug acarbose. The greater surface area of nanoparticles exposes the various groups of curcumin for blocking the binding sites of enzymes. This strategy may be helpful in designing curcumin as a potent therapeutic agent against diabetes mellitus. Further, the molecular interactions of curcumin with α-amylase, α-glucosidase, β-glucosidase, and polyphenol oxidase were assessed by analyzing the plausible binding modes of curcumin in the binding pocket of each receptor. The best binding mode of curcumin was used to make complexes with the target proteins and their stability was confirmed by 50 ns MD simulation.

Curcumin nanocapsules effect in apoptotic processes, gene expression, and cell cycle on Hep-G2 cell lines.

Curcumin has antioxidant and antiproliferative properties, and its therapeutic effect must be considered. Nanocurcumin capsules showed a potential increase against in vitro biological cancer. This study sought to determine how curcumin nanoparticles and nanocapsules affected the expression of p53, Bcl-2, Bax, and Bax in a liver cancer cell line (Hep-G2). Mechanisms of apoptosis were also examined in this cell line. This study used quantitative real-time polymerase chain reaction (qRT-PCR) to analyze the p53, Bcl-2, Bax, and Caspase-3 gene pathways and to evaluate the molecular mechanisms responsible for the efficacy of curcumin nanoparticles (CNPs) and curcumin nanocapsules (CNCs) against liver cell lines. Flow cytometry was used to check for signs of apoptosis and the cell cycle. Curcumin nanocapsules produced by the ball milling process at 90min significantly boosted the populations of apoptotic cells in a dose- and time-dependent manner. The mRNA expression analysis revealed that the proapoptotic Bax, Caspase-3, and the tumor suppressor gene p53 were upregulated throughout the process started by curcumin nanocapsules and decreased in the Bcl-2/Bax ratio. This research provides a fresh understanding of the molecular mechanisms behind the liver cancer-fighting abilities of curcumin nanoparticles. Curcumin nanocapsules produced through a unique mechanical technique can be used as an anticancer agent.

Molecular study of Shigella dysenteriae Aminoglycoside Resistance Genes Isolated from Children and its Expression Under the Influence of Curcumin Nanoparticle

Molecular study of <i>Shigella dysenteriae</i> Aminoglycoside Resistance Genes Isolated from Children and its Expression Under the Influence of Curcumin Nanoparticle

MULTIFUNCTIONAL NANOSYSTEMS BASED ON TWO FLUORESCENT DYES, DOXORUBICIN AND CURCUMIN

The aim of the work was to review the literature data regarding the prospects for the creation and use of multifunctional fluorescent two-dye nanosystems, which enable investigating the distribution of fluorescent components with significant acceleration of the study and introduction of nanomedicines into practice. Special attention is paid to the use of two substances with hydrophobic and hydrophilic properties in one nanoparticle (NP), capable of penetrating a living cell. The method of fluorescence confocal microscopy enables observation of the nanoscale dynamics of distribution and stability of drugs over time. The concomitant use of doxorubicin (DOX) and curcumin (CUR) in single nanoparticle causes synergism in the action of medical drugs, and their own fluorescence makes it possible to use them as multifunctional fluorescent nanosystems. Results. Data from the literature indicate that the use of two or more fluorescent dyes provide an advantage over other, more expensive methods when studying the penetration and distribution of NPs in living samples. The use of nanocarriers is an effective way to significantly increase the bioavailability of those drugs, which are poorly soluble in water. A promising direction of nanomedicine is the creation of complex bio-compatible multifunctional nanomaterials based on several active drugs, with the simultaneous use of their enhancers and the strategy of active targeting. Such recent structures enable targeted and controlled penetration of medicinal compounds into the sites of localization of pathological processes, reducing the toxicity of drugs to normal cells. Conclusions. The use of the fluorescence microscopy method, as exemplified by the two dyes, DOX and CUR, enables to trace the stages of interaction of loaded DOX and CUR nanoparticles with cultured cells, and their release from NPs to determine their amount and localization in organelles cells.

Influence of Spraying Nano-Curcumin and Nano-Glycyrrhizic Acid on Resistance Enhancement and Some Growth Parameters of Soybean (Glycine max) in Response to Tetranychus urticae Infestation and Drought Stress.

Modern nanotechnology has been credited as one of the most significant inventions of the 21st century. Many agricultural disciplines have been affected by nanotechnology in agriculture. Pest control based on natural compounds needs to be enhanced, and enhancing plant growth under climate change conditions, with increasing periods of drought in many countries, is a very vital aim. Thus, the effect of curcumin nanoparticles (Cu-NPs) and glycyrrhizic acid nanoparticles (GA-NPs) as a foliar application under water deficit on natural infestation with the two-spotted spider mite Tetranychus urticae, plant growth and yield, anatomical and chemical parameters were investigated during this study. The obtained results revealed that drought stress over the two studied seasons significantly increased the population of T. urticae and decreased all morphological and yield characteristics. The application of three mM GA-NPs reduced the mite population average by 39% while using the same concentration of Cu-NPs caused a 33.9% reduction percentage under drought stress. Using 1 mM GA-NPs gave the highest averages of plant height, number of branches, and leaves/plant fresh and dry weight. Moreover, the number of pods, 100 seed weight and seed yield (kg/ha) increased significantly as a result of spraying with GA-NPs under water deficit. From the results, water deficit decreased the values of the leaf and stem anatomical parameters. Treatment with Cu-NPs or GA-NPs under drought stress increased the thickness of mid-vein, xylem, and phloem tissues. Likewise, such treatment increased stem diameter due mainly to the increase in the thickness of cortex, phloem, and xylem tissues compared with the control. Spraying plants with GA-NPs at 1 mM increased the percentages of nitrogen, phosphor, and potassium in seeds in addition to total chlorophyll. Moreover, glutamate, aspartate, leucine, arginine, Lysine, glycine, tyrosine, tryptophan, and methionine concentrations did not differ significantly (p > 0.05) in response to all the studied levels of Cu-NPs or GA-NPs either under normal irrigation or drought condition. In light of these findings, researchers and producers should apply and test both Cu-NPs and GA-NP as nano-fertilizer natural sources on economically viable crops.