Nanoemulsion System Research Articles

Nanoemulsion: A novel delivery approach for thermosensitive IgG on inhibiting milk fat oxidation

IgG, a biologically active substance in bovine colostrum, is easily inactivated during heat treatment and edible process to lose its biological activity. Nanoemulsion can effectively protect IgG to maintain its biological activity from injurious treatment. In this study, a food-grade nanoemulsion system was developed to protect IgG from heat and acid damage. It can be found that the residual rate of nanoemulsion-protected IgG reaches 87.1 % after 10 min at 72 °C. After 5 min at 82 °C, the residual rate of IgG in nanoemulsion was 18.7 % higher than that in PBS. In the simulated gastric fluid at pH 2.0, the residual rate of IgG in the nanoemulsion reacted for 4 h was 21.5 % higher than that in PBS. It indicated that nanoemulsion system can improve the heat and acid resistance of IgG compared with others, which is attributed to the lowest water activity of nanoemulsion. The contents of hydroperoxide and malondialdehyde in the milk after storage for 72 h with nanoemulsion-protected IgG were 0.12 meq/kg and 0.04 mg/kg, respectively, less than that of PBS-protected IgG. IgG is protected by nanoemulsion can effectively protect its activity during processing, which provides a theoretical basis for its direct application in liquid milk.

Synergistic Combination of Irinotecan and Rapamycin Orally Delivered by Nanoemulsion for Enhancing Therapeutic Efficacy of Pancreatic Cancer

In recent years, combining different types of therapy has emerged as an advanced strategy for cancer treatment. In these combination therapies, oral delivery of anticancer drugs is more convenient and compliant. This study developed an irinotecan/rapamycin-loaded oral lecithin-based self-nanoemulsifying nanoemulsion preconcentrate (LBSNENPir/ra) and evaluated its synergistic combination effects on pancreatic cancer. LBSNENP loaded with irinotecan and rapamycin at a ratio of 1:1 (LBSNENPir10/ra10) had a better drug release profile and smaller particle size (<200 nm) than the drug powder. Moreover, LBSNENPir10/ra10 exhibited a strong synergistic effect (combination index [CI] < 1.0) in cell viability and combination effect studies. In the tumor inhibition study, the antitumor activity of LBSNENPir10/ra10/sily20 against MIA PaCa-2 (a human pancreatic cancer cell line) was significantly increased compared with the other groups. When administered with rapamycin and silymarin, the area under the curve and the maximum concentration of irinotecan significantly improved compared with the control. We successfully developed an irinotecan/rapamycin-loaded oral self-nanoemulsifying nanoemulsion system to achieve treatment efficacy for pancreatic cancer.

Multi-drug loaded eugenol-based nanoemulsions for enhanced anti-mycobacterial activity.

Tuberculosis is one of the oldest bacterial infections known to mankind caused by Mycobacterium tuberculosis. The aim of this research is to optimize and formulate a multi-drug loaded eugenol based nanoemulsion system and to evaluate its ability as an antimycobacterial agent and its potential to be a low cost and effective drug delivery system. All the three eugenol based drug loaded nano-emulsion systems were optimized using response surface methodology (RSM)-central composite design (CCD) and were found stable at a ratio of 1 : 5 (oil : surfactant) when ultrasonicated for 8 minutes. The minimum inhibitory concentration (MIC) values against strains of Mycobacterium tuberculosis highly proved that these essential oil-based nano-emulsions showed more promising results and an even improved anti-mycobacterium activity on the addition of a combination of drugs. The absorbance of 1st line anti-tubercular drugs from release kinetics studies showed a controlled and sustained release in body fluids. Thus, we can conclude that this is a much more efficient and desirable method in treating infections caused by Mycobacterium tuberculosis and even its MDR/XDR strains. All these nano-emulsion systems were stable for more than 3 months.

Physical Stability of Sea Buckthorn Oil Nanoemulsion with Tween 80 Variations

Sea buckthorn oil (SBO) contains fatty acids that have an anti-aging effect. The delivery system ofSBO can be improved by a nanoemulsion system. The purpose of this study was to determine thephysical stability of SBO nanoemulsion (NSB) with Tween 80 variation. NSB was made with 1% SBOand Tween 80 variation of 35% (FI); 40% (FII) and 45% (FIII). NSB was stored in a climatic chamberat 30 ± 2°C and RH of 65%±5% for 90 days. Samples were taken before storage on the 15th, 30th,60th, and 90th days. Evaluations were carried out including appearance, particle size, polydispersityindex (PI), transmittance percentage (TP), pH, and viscosity. Quantitative data were analyzed withmultivariate analysis. The result showed that NSB formulas were yellowish-orange and clear. Thedroplet size in FII and FIII was stable (p &gt; 0.05) with a range of 12-15 nm with PI &lt; 0,5. All formulashad TP &gt; 95% with a pH of 7.50–7.6. The viscosity of all formulas was 1.2-1.9 dPa.s and characterizedas Newtonian. Increasing the concentration of tween 80 can reduce droplet size and PI. Tween 80 at aconcentration of 35-45% produces sea buckthorn nanoemulsion which is stable for 90 days of storage.

Comparison of antibacterial and antioxidant potentials of pure and nanoemulsified Nepeta pogonosperma essential oil.

The current study aimed to investigate the antiradical and antibacterial potential of pure and its nanoemulsified (NNE) Nepeta pogonosperma essential oil (PNE). Antimicrobial activity of the essential oil against two Gram-positive (E. faecalis and B. cereus) and two Gram-negative (M. catarrhalis and K. pneumonia) food-related pathogens during 60-day storage was investigated based on disc diffusion, minimum inhibition concentration (MIC), and minimum bactericidal concentration (MBC). The chemical compounds of Nepeta essential oil were estimated by GC/MS. The physical properties of the nanoemulsion including polydispersity index (PDI), mean particle diameter, and viscosity were also determined. 4aα,7α,7aβ-Nepetalactone (46.31%), 1,8-cineole (23.13%), and (Z)-α-bisabolene (4.01%) were the main compounds of this essential oil. The Nepeta nanoemulsion had a mean droplet diameter of 254.07 nm, PDI of 0.281, and viscosity of 0.887 cP. NNE had stability for up to 60 days. The PNE showed a higher IC50 value than NNE (p < .05). During storage, the antiradical performance of both PNE and NNE was decreased (p < .05). However, emulsification was successful to control this decreasing trend. E. faecalis was the most susceptible bacteria to PNE and NNE, while the lowest inhibition zone was obtained for K. pneumoniae. At the first time, the antibacterial effect of PNE was more than NNE. However, over time nanoemulsion became more successful in maintaining its antibacterial effect. Overall, the incorporation of Nepeta pogonosperma essential oil into a nanoemulsion system can be a promising system to maintain the bioactivity of the essential oil for a longer time.

Formulation and Physical Characterization of Sunflower Oil Nano Emulsion Containing Cinnamon Bark Extract

This research developed a sunflower oil nano emulsion containing cinnamon bark extract. Cinnamon bark contains many bioactive compounds that provide various pharmacological benefits, including antioxidant properties. The bark extract, when developed into a nano emulsion system using sunflower oil, improves the antioxidant property. The maceration method using ethanol 95% was used to extract the cinnamon bark and then further developed into the nano emulsion system using between 80 as surfactant and PEG 400 as cosurfactant. The optimum nano emulsion formula contains 4% sunflower oil, and 30% PEG 400. The sunflower oil nano emulsion is transparent, with a globule size of 102 ± 32 nm. The optimum formula of cinnamon bark nano emulsion was achieved with 0.25% extract. This shows good physical characteristics and stability.&#x0D; Keywords: nanoemulsion, cinnamon bark, sunflower oil

Effect of Nanoemulsion Containing Enterocin GR17 and Cinnamaldehyde on Microbiological, Physicochemical and Sensory Properties and Shelf Life of Liquid-Smoked Salmon Fillets.

The spoilage of liquid-smoked salmon represented a serious restriction for shelf life, due to the loss of taste, smell, color and consistency in product quality. The objective of this study was to investigate the feasibility of applying a nanoemulsion delivery system co-encapsulated enterocin Gr17 and essential oils (EOs) to the refrigerated storage of liquid-smoked salmon. The synergistic inhibiting effects of enterocin Gr17 and EOs were evaluated, a nanoemulsion delivery system with the optimal combination was developed, and the evolution of the microbiological, physicochemical, and sensory properties of liquid-smoked salmon fillets were analyzed during a 49-day period of refrigerated storage. The results showed that the combination of enterocin Gr17 and cinnamaldehyde essential oil (CEO) displayed the strongest synergistic inhibiting effect on foodborne pathogens. A nanoemulsion system incorporating enterocin Gr17 and CEO was successfully developed and presented a broad spectrum of activity against most of the tested bacteria. A nanoemulsion system incorporating enterocin Gr17 and CEO (CO-NE) could significantly inhibit the growth of microflora, suppress the accumulation of total volatile basic nitrogen (TVB-N) and thiobarbituric acid reactive substance (TBARS), and maintain better color, texture, and sensory profiles during smoked salmon storage at 4 °C. Overall, from a microbiological, physicochemical, and sensory point of view, the CO-NE treatment could extend the shelf life to 42 days and maintain the relatively low TVB-N value (≤15.38 mg/100 g), TBARS value (≤2.51 mg MDA/kg), as well as a relatively high sensory score (≥5.83) during the whole storage period. Hence, a nanoemulsion system incorporating enterocin Gr17 and CEO could be a promising bio-preservative technology and alternative to the conventional processes used for improving the safety and quality of chilled liquid-smoked salmon.

Graffiti coating eco-remover developed for sensitive surfaces by using an optimized high-pressure homogenization process

Nanostructured fluids (NSFs), such as water-in-oil (w/o) nanoemulsions (NEs), comprise one of the best examples of new, ecological products dedicated to removing various graffiti coatings (so-called eco-removers) from sensitive surfaces without causing damage underneath. These contemporary acceptable formulations are intended to be more efficient in removing undesirable graffiti coatings than traditional organic solvent-based preparations. In this paper, systematic studies were performed on the selective and controlled removal of paint and ink coatings from substrates that were sensitive to mechanical cleaning by using innovative graffiti coating removers in the form of “brush on, wipe off” detergents. The w/o NEs were fabricated by using a high-pressure homogenization process (HPH) from the following ingredients from sustainable sources of raw materials: esterified used cooking oils (Oil-PEG-8-ester), ethyl lactate (EL), water and saccharide-derived surfactants, such as alkyl polyglucosides (APGs), which have excellent wetting and detergent activities. The most efficient formulations achieved during the HPH process were determined by optimization through response surface methodology (RSM). The optimized w/o nanoemulsions were tested for the selective removal and wetting ability of graffiti coatings from selected sensitive surfaces, such as plastics, ceramics, or painted metal surfaces. Additionally, in studies of interaction with graffiti coatings, evaluating their surface properties (topography, wettability) on a real paint coating allowed for the assessment of the mechanism of graffiti paint removal by the proposed ecological remover. Using the "brush on, wipe off" cleaning procedure based on the developed w/o nanoemulsion systems has the potential to remove safely rough and heterogeneous graffiti coatings. Therefore, nanotechnological detergents are an outstanding environmentally friendly alternative to conventional graffiti coating removers.

Effect of nanoemulsion to minimise undesirable odour and colour of cinnamon bark oil and to improve sensory properties of refrigerated Asian seabass fillets

SummaryThe use of cinnamon bark oil (CBO) to inhibit fish spoilage causes undesirable changes in sensory properties of fish fillets. This research prepared CBO nanoemulsion (CBON) and studied its effects on the volatile profile and sensory properties of Asian seabass fillets. Changes in volatile profile, colour and odour scores, and overall likeness scores of CBON‐treated fillets were determined during storage in comparison to the control (CTRL) and bulk cinnamon bark oil (BCBO)‐treated ones. After treatment, CBON and BCBO were able to mask the natural odour of fish fillets. However, CBON did not change the fillets' colour. During storage, CBON‐treated fillets contained relatively higher cinnamaldehyde content and higher colour score but less cinnamon oil‐like odour score than those of BCBO‐treated ones. CBON‐treated fillets also had higher acceptance scores than those of CTRL and BCBO‐treated fillets after storage. These results demonstrated the efficiency of the nanoemulsion system to minimise undesirable effects of CBO and maintain sensory acceptance of the fillets during storage.

Oral self-emulsifying nanoemulsion systems for enhancing dissolution, bioavailability and anticancer effects of camptothecin

This research aimed to prepare a camptothecin (CPT) loaded self nano emulsifying drug delivery system (SNEDDS) that would improve the oral therapeutic efficacy of CPT. 32 full factorial design was employed, and the effect of the amount of oil (X1) and surfactant mixture (X2) was systematically investigated on percentage transmittance (Y1), self-emulsification time (Y2), and percentage cumulative drug release (Y1). Pseudo-ternary phase diagrams were constructed at different km values (1, 2, and 3). In total, 13 experimental runs (LF–1 to LF–13) were prepared at km = 3, evaluated, and converted to solid free-flowing granules (S–SNEDDS) using neusilin® US2. LF–10 comprising 12.5% omega oil and 40% cremophor-RH40 and labrafil-M2125 had nanosizing (<200 nm), high % transmittance (98.42 ± 1.82%), and lower self-emulsification time (21 ± 0.38 Sec). Nano-sized droplets were spherical, as revealed by the TEM study. FT-IR spectra showed no incompatibilities, whereas DSC and XRD results confirmed CPT's amorphous state and molecular dispersion in SNEDDS. In-vitro dissolution studies demonstrated significant (P < 0.05) improvement in CPT release characteristics from S–SNEDDS (SF–10). in-silico studies confirmed hydrogen bond interaction between an amino group of CPT and neusilin oxygen. S-CPT-10 significantly (P < 0.05) inhibited the proliferation of BEWO placental cancer cells more than CPT. It induced apoptosis in cancerous cells, revealed by DAPI, Annexin-V/PI staining, and mitochondrial potential measurement. Remarkably, oral administration of CPT–SNEDDS resulted in a 17-fold increase in bioavailability compared to plain CPT. Thus, SNEDDS could be utilized as a potential carrier for CPT delivery.

Development and characterization of amphotericin B nanoemulsion-loaded mucoadhesive gel for treatment of vulvovaginal candidiasis

Despite being recognized as the “gold standard” for treating azole-resistant vulvovaginal candidiasis, amphotericin B (AmB), an amphoteric molecule, has not been widely used due to serious issues with solubility and permeability. In light of the aforementioned, the objective of the present study was to increase AmB's therapeutic efficacy by formulating it into an o/w nanoemulsion (AmB-NE) system. Furthermore, to facilitate AmB-NE's retention within the vaginal cavity, it was loaded into a mixture of Carbopol® 974P and Aloe vera-based gel (CA gel). Briefly, in the present study, a kinetically stable batch of formulated AmB-NE having a globule size of 76.52 ± 3.11 nm, PDI of 0.342 ± 0.032, and zeta potential of -22.32 ± 0.88 mV was incorporated into the CA gel base. This AmB-NE loaded gel (AmB-NE gel) exhibited a non-Fickian/anomalous diffusion from the hydrophilic matrix. The texture analysis of AmB-NE gel revealed that the prepared gel was a non-drip, soft, easy to spread, and sufficiently cohesive gel that could reside in the vaginal cavity, which was confirmed by our ex-vivo retention test, which revealed that AmB-NE loaded gel could stay in the vaginal cavity for approximately 11 h. Ex-vivo skin permeation studies revealed that AmB-NE is 4.26 times more permeable than AmB-coarse gel, implying that AmB-NE facilitates AmB entry into the vaginal epithelial layers. Furthermore, in-vivo vaginal lavage studies revealed that AmB-NE gel permeated 7.03-fold more than AmB-coarse gel. Prepared AmB-NE gel was stable in refrigerated condition and showed no histopathological toxicity. Thus, the present study suggests that AmB-NE gel could eliminate the existing problem of AmB and that it could serve as an alternative option to treat vulvovaginal candidiasis.

Application of Nanotechnology Incorporated with Natural Ingredients in Natural Cosmetics

Technology and environmental innovations always have a growing emphasis and have affected various industries. Since the cosmetics industry is highly competitive and consumers always desire innovations in products, the cosmetics industry tends to launch new products to satisfy customer desires without exception. Recently, in the area of cosmeceuticals, the application of nanotechnology has been attempting to increase; however, it seems to be tackling certain disadvantages compared to traditional products. Cosmeceuticals based on nanotechnology offer the benefits of product differentiation, improved bioavailability, and prolonged effects of active ingredients. These are widely used as a regulated delivery medium for cosmeceuticals, such as shampoo, nail enamels, lotions, hair serums, and conditioners. Their wide application is attributed to their intrinsic properties, such as rapid penetration, stability, hydrating power, and texture. The ability of the nanoemulsion system to form small- and large-surface nanoparticles enable the bioactive components to be transported into the skin more effectively. Furthermore, natural cosmetics are a general term that refers to all preparations for external conditioning and beautifying of the body from ingredients produced by or found in nature. The purpose of this review is to understand the nature of natural cosmetics which incorporate nanotechnology for better encapsulation and a better drug delivery system. The importance of innovations in the cosmetic industry is also discussed.

Cinnamon oil nanoemulsion as a novel nanocarrier for bleomycin amplifies its apoptotic effect on SKOV-3 ovarian cancer cells

Loading the chemotherapeutic agents in nanoemulsions system has recently gained attraction in medicine due to their ability to improve the drug’s efficacy and reduce its adverse effects. In this context, here, we loaded bleomycin (BLM) in nanoemulsion (NE) consisting of cinnamon oil in order to evaluate its antineoplastic effect on the SKOV-3 cells. The produced NE formulas were physically characterized by the zetasizer. The cytotoxic activities of BLM and NE formulas were examined by CCK-8 kit, Coomassie blue staining for the visualization of the morphological changes, Annexin V-FITC for identifying apoptosis and cell death detection ELISA plus kit for DNA fragmentation measurement. The average droplet diameter of the blank NE (450.90±1.57 nm) was increased when loaded with BLM (522.57±0.85 nm) while the magnitude of the negative zeta potential of the loaded formula (0.381±0.003 mV) was less than the blank NE (1.01±0.020 mV). The potential cytotoxicity of the BLM-NE was significantly greater than the toxicities of the free BLM and blank NE. The blank NE and BLM-NE have the greatest apoptotic effect and higher enrichment factor compared to free BLM. Loading BLM in NE based on cinnamon oil has considerably improved its efficacy as an anticancer drug on the SKOV-3 cells.

Supramolecular assemblies of a newly developed indole derivative for selective adsorption and photo-destruction of perfluoroalkyl substances

Per-/polyfluoroalkyl substances (PFASs) contamination has caused worldwide health concerns, and increased demand for effective elimination strategies. Herein, we developed a new indole derivative decorated with a hexadecane chain and a tertiary amine center (named di-indole hexadecyl ammonium, DIHA), which can form stable nanospheres (100–200 nm) in water via supramolecular assembly. As the DIHA nanospheres can induce electrostatic, hydrophobic and van der Waals interactions (all are long-ranged) that operative cooperatively, in addition to the nano-sized particles with large surface area, the DIHA nanocomposite exhibited extremely fast adsorption rates (in seconds), high adsorption capacities (0.764–0.857 g g−1) and selective adsorption for perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS), outperformed the previous reported high-end PFASs adsorbents. Simultaneously, the DIHA nanospheres can produce hydrated electron (eaq−) when subjected to UV irradiation, with the virtue of constraining the photo-generated eaq− and the adsorbed PFOA/PFOS molecules entirely inside the nanocomposite. As such, the UV/DIHA system exhibits extremely high degradation/defluorination efficiency for PFOA/PFOS, even under ambient conditions, especially with the advantages of low chemical dosage requirement (μM level) and robust performance against environmental variables. Therefore, it is a new attempt of using supramolecular approach to construct an indole-based nanocomposite, which can elegantly combine adsorption and degradation functions. The novel DIHA nanoemulsion system would shed light on the treatment of PFAS-contaminated wastewater.

Novel Curcumin-Encapsulated α-Tocopherol Nanoemulsion System and Its Potential Application for Wound Healing in Diabetic Animals.

Objective This project was aimed at formulating a novel nanoemulsion system and evaluating it for open incision wound healing in diabetic animals. Methods The nanoemulsions were characterized for droplet size and surface charge, drug content, antioxidant and antimicrobial profiling, and wound healing potential in diabetic animals. The skin samples excised were also analyzed for histology, mechanical strength, and vibrational and thermal analysis. Results The optimized nanoemulsion (CR-NE-II) exhibited droplet size of26.76 ± 0.9 nm with negative surface charge (−10.86 ± 1.06 mV), was homogenously dispersed with drug content of68.05 ± 1.2%, released almost82.95 ± 2.2%of the drug within first 2 h of experiment with synergistic antioxidant (95 ± 2.1%) and synergistic antimicrobial activity against selected bacterial strains in comparison to blank nanoemulsion, and promoted significantly fast percent reepithelization (96.47%). The histological, vibrational, thermal, and strength analysis of selected skin samples depicted a uniform and even distribution of collagen fibers which translated into significant increase in strength of skin samples in comparison to the control group. Conclusions The optimized nanoemulsion system significantly downregulated the oxidative stress, enhanced collagen deposition, and precluded bacterial contamination of wound, thus accelerating the skin tissue regeneration process.

Investigation of Factors Influencing Formation of Nanoemulsion by Spontaneous Emulsification: Impact on Droplet Size, Polydispersity Index, and Stability.

Interest in nanoemulsion technology has increased steadily in recent years for its widespread applications in the delivery of pharmaceuticals, nutraceuticals, and cosmeceuticals. Rational selection of the composition and the preparation method is crucial for developing a stable nanoemulsion system with desired physicochemical characteristics. In the present study, we investigate the influence of intricate factors including composition and preparation conditions that affect characteristic parameters and the stability of the nanoemulsion formation prepared by the spontaneous emulsification method. Octanoic acid, capryol 90, and ethyl oleate were selected to represent oil phases of different carbon–chain lengths. We explored the impact of the addition mode of the oil–Smix phase and aqueous phase, vortexing time, Km (surfactant/cosurfactant) ratio, and the replacement of water by buffers of different pH as an aqueous system. The phase behavior study showed that the Smix phase had a significant impact on the nanoemulsifying ability of the nanoemulsions composed of oil phases of varying carbon-chain lengths. The mode of mixing of the oil–Smix phase to the aqueous phase markedly influenced the mean droplet size and size distribution of the nanoemulsions composed of oil phases as capryol 90. Vortexing time also impacted the mean droplet size and the stability of the generated nanoemulsion system depending on the varying carbon-chain length of the oil phase. The replacement of the water phase by aqueous buffers of pH 1.2, 5.5, 6.8, and 7.4 has altered the mean droplet size and size distribution of the nanoemulsion system. Further, the Km ratio also had a significant influence on the formation of the nanoemulsion system. The findings of this investigation are useful in understanding how the formulation composition and process parameters of the spontaneous emulsification technique are responsible for affecting the physicochemical characteristics and stability of the nanoemulsion system composed of oil of varying carbon-chain (C8-C18) length.

Repurposing maduramicin as a novel anticancer and anti-metastasis agent for triple-negative breast cancer as enhanced by nanoemulsion

Triple-negative breast cancer (TNBC) is featured by aggression and metastasis and remains an unmet medical challenge due to high death rate. We aimed to repurpose maduramicin (MAD) as an effective drug against TNBC, and develop a nanoemulsion system to enhance anticancer efficacy of MAD. MDA-MB-231 and 4 T1 cells were used as in vitro model, and cell viability was determined by performing cell counting kit-8 and a colony-formation assay. Furthermore, MAD loaded nanoemulsion (MAD-NEs) was manufactured and characterized by a series of tests. The anticancer and anti-metastasis mechanism of MAD-NEs were assessed by performing cell cycle, apoptosis, wound-healing, transwell assay and Western blotting assays. Herein, MAD was firstly demonstrated to be an effective agent to suppress growth of TNBC cells. Subsequently, the optimized MAD-NEs were shown to have stability and high encapsulation efficiency, and could arrested cells in G0/G1 phase and induced apoptosis in TNBC cells. More importantly, MAD-NEs significantly impeded the metastasis of tumor cells, which was further demonstrated by the significant altered expression of epithelial-mesenchymal transition and extracellular matrix markers in vitro and in vivo. Moreover, compared to MAD, MAD-NEs exhibited higher efficacy in shrinking breast tumor size and repressing liver and lung metastasis in vivo, and showed excellent biocompatibility in tumor-bearing mice. The successfully prepared MAD-NEs are expected to be harnessed to suppress tumor growth, invasion and metastasis in the battle against malignant TNBC.

Improving oxidative stability of edible oils with nanoencapsulated orange peel extract powder during accelerated shelf life storage

Nanoemulsion systems were employed to encapsulate the orange peel extract obtained from conventional maceration and novel ultrasound assisted extraction technology. Different wall materials maltodextrin (MD), whey protein isolate (WPI) and complex maltodextrin and whey protein isolate (MD/WPI) (1:1) were used in order to enhance the nanoemulsion stability, protect the bioactive core from degradation and control the release of bioactive compounds. The freeze drying technology was then used to obtain powder encapsulates with encapsulation efficiency and loading capacity of MD, WPI and MD/WPI as 89.3,91.2,95.6% and 95.3,94.4 and 93.1% respectively which depicts that encapsulation has taken place properly. All emulsions obtained were in nanoscale range with average hydrodynamic diameter as 105.0, 201.82, 215.12 and 298.82 for primary W/O emulsion, MD, WPI and MD/WPI stabilised emulsions respectively. These encapsulates were then incorporated in soyabean and mustard oil at different concentration 100, 200 and 300 ppm and compared with free extract and synthetic antioxidant BHT to enhance storage life by controlling the oxidative changes during accelerated shelf life storage at 60o C for 24 days. The results obtained concluded that nanoencapsulated extracts performed better in prevention of oxidative indices than the free extract. However, nanocapsules encapsulated by MD/WPI exhibited higher protection of oils during storage than the maltodextrin and whey protein isolate.

Enhancement of Storage Stability and Masking Effect of Curcumin by Turmeric Extract-Loaded Nanoemulsion and Water-Soluble Chitosan Coating.

This study focused on improving curcumin stability in various pHs and NaCl concentrations and reducing the strong scent of turmeric by the nanoemulsions system and further coating with water-soluble chitosan (WSC). Turmeric extract-loaded nanoemulsions (TE-NEs) were firstly prepared by mixing an oil phase containing turmeric extract, MCT oil, and lecithin, and an aqueous phase containing tween 80 using an ultrasonication method. TE-NEs were further coated with WSC in the ratio of TE-NEs and WSC (1:1 to 1:10). The optimum WSC-TE-NEs exhibited an average particle size of 182 nm, a PDI of 0.317, and a zeta potential of +30.42 mV when WSC-TE-NEs were prepared in the ratio of 1:1. The stability of the WSC-TE-NEs was also assessed by determining the remained curcumin content. The remained curcumin contents of the TE-NEs and the WSC-TE-NEs were higher than that of the turmeric extract (TE) at pH 2~7 and NaCl concentrations of 100~400 mM. Fourier transform infrared (FT-IR) spectra, transmission electron microscope (TEM), and confocal laser scanning microscope (CLSM) images confirmed that the TE-NEs were successfully encapsulated with a WSC coating. As a result of GC analysis, the content of aromatic-turmerone was significantly decreased in the TE-NEs and the WSC-TE-NEs compared to the pristine TE, but there was no significant difference between the TE-NEs and the WSC-TE-NEs. These results suggest that water-soluble chitosan-coated nanoemulsions may be suitable for improving the chemical stability and masking effect of curcumin to facilitate its application in food.

Food-Grade Quercetin-Loaded Nanoemulsion Ameliorates Effects Associated with Parkinson's Disease and Cancer: Studies Employing a Transgenic C. elegans Model and Human Cancer Cell Lines.

A nanosized food-grade quercetin-loaded nanoemulsion (QNE) system comprising capmul MCM NF (oil) and cremophor RH 40 (surfactant) was developed using a high-speed homogenization technique. The developed QNE was studied for its significant neuroprotective (anti-Parkinsonism) and cytotoxicity (anticancer) effects against Caenorhabditis elegans (C. elegans) strains and human cancer cells, respectively. HR-TEM studies revealed that the QNE was spherical with a mean globule size of ~50 nm. Selected area electron diffraction (SAED) studies results demonstrated that QNE was amorphous. In vivo results show that QNE potentially reduced the α-Syn aggregation, increased mitochondrial and fat content, and improved the lifespan in transgenic C. elegans strain NL5901. QNE significantly downregulated the reactive oxygen species (ROS) levels in wild-type C. elegans strain N2. In vitro results of the MTT assay show that QNE significantly exhibited chemotherapeutic effects in all treated human cancer cells in an order of cytotoxicity: HeLa cells > A549 cells > MIA PaCa-2 cells, based on the IC50 values at 24 h. Conclusively, the QNE showed improved solubility, targetability, and neuroprotective effects against the PD-induced C. elegans model, and also cytotoxicity against human cancer cells and could be potentially used as an anti-Parkinson’s or anticancer agent.