Insufficience of osteogenesis and antimicrobial effect have been still impacted the long term clinical success rate of dental implants. A nanostructured titanium surface prepared by hydrothermal treatment with H2O2 was evaluated on its osteoblastic viability and antibacterial effect. Samples were divided into 2 groups: untreated pure titanium surface (Ti) and a nanostructured titanium surface (NT). The antibacterial activities against S.mutans and C.albicans were measured by film applicator coating assay, as well as the live/dead bacteria stain. The osteoblastic viability was investigated by SEM and MTT assay. Results showed that the active microbia on NT was reduced at 24h (P<0.05) significantly according to the live/dead bacteria stain and film applicator coating assay, which could also enhance the osteoblast viability. Therefore, a nanostructured titanium surface exhibits good antibacterial activity on S.mutans and C.albicans, and promoting osteoblast viability, which will be a potential kind of dental implant material.