Bacterial blight disease of pomegranate caused by Xanthomonas axonopodis pv. punicae decrease crop productivity. X. axonopodis was isolated from infected fruits, stem and leaves collected from Pravara area on NAS medium. Pure culture was obtained by regular subculture and maintained on NAS medium. Trichoderma harizanium fungal biomass was generated for nanoparticle synthesis on liquid PDA medium. The aqueous cell free culture filtrate (CFCF) obtained by filtration. Nanoparticles were synthesized using 50 ml of aqueous CFCF in flasks by stirring appropriate amount of, CuSO4 (1–2 mM) solution in dark at 45 °C. Silver nanoparticles were synthesized using Trichoderma viride. SEM analysis proved silver nanoparticle size in the range 5–10 µm. Spread plate and colony count techniques proved significant antibacterial activity of silver nanoparticles against Xanthomonas axonopodis.