Nanobubbles Research Articles

Evaluation of industrial performance of a new three phase fluidized bed flotation column-Based on product size characterization

There are still a large number of minerals processed by flotation every year, so it is extremely important to improve the flotation efficiency in industrial production. This study established an experimental test platform for new three-phase fluidized bed flotation column (TFC). The effects of different operating conditions and equipment parameters (apparent gas velocity, apparent liquid velocity, and filling height) on separation effect of coal slime were investigated. Relationship between turbulence intensity inside TFC and the flotation effect of coal slime with different particle sizes was analyzed. Results show that turbulence intensity in fluidized environment can be influenced by adjusting operating conditions to increase the probability of adhesion and collision of fine-grained particles with air bubbles. Differences of particle size distribution in the radial and axial directions of TFC are influenced by settling velocity. Increasing circulation volume can increase residence time of particles in flotation process. Micro and nano bubbles have excellent characteristics which are beneficial to particle collision and adhesion. By adjusting the parameters, it can meet needs of mineral separation and mineralization environment and realize effective recovery of slime with different particle sizes. This study was conducted by analyzing the flotation of fine particles by TFC during industrial platform testing. By evaluating the industrial performance of TFC, it provides a reference for future industrial large-scale applications.

Advanced oxidation of Arsenic(III) to Arsenic(V) using ozone nanobubbles under high salinity

Arsenic, a highly toxic element, is present in various water resources as As(III) and/or As(V). The removal of As(V) using adsorption is considered to be easier than the removal of As(III). In this work, we report the oxidation of As(III) to As(V) using ozone nanobubbles. Ozone has been widely used in the advanced oxidation process (AOP) to remove organics and inorganics in wastewater. The major advantage of ozone nanobubbles is their enhanced half-life, which leads to higher ozone solubility in water. In addition, the rate of mass transfer by using nanobubbles can be enhanced drastically. The effect of ozone flow rates, initial As(III) concentrations, pH levels, and the presence of salt is systematically studied in this work. The present results revealed that ozone nanobubbles positively impacted the oxidation of As(III) to As(V). AOP process based on O3 NBs (nanobubbles) was most efficient with 99 % oxidation rates of 1 ppm of As(III) within 20 minutes at 6.5 ppm ozone concentrations. An acidic pH of 3–7 promoted quick oxidation due to the high mass transfer coefficient of ozone nanobubbles. About ∼70 % degradation of As(III) to As(V) was achieved with 0.10854 min-1 rate constant at acidic (pH = 3) compared to ∼39 % degradation with 0.046 min-1 rate constant at pH =11. The presence of salt (∼50 mM) in the solution not only hindered the ozone mass transfer coefficient but also reduced the stability of nanobubbles. The detection of reactive oxygen species, particularly hydroxyl radical was unravelled by utilizing 2-propanol as a scavenger.

Relationship between chlorine decay and nanobubble application in secondary treated wastewater.

There has been numerous research on the uses of treated wastewater that needs chlorine disinfection, but none have looked at the impacts of injecting nanobubbles (NBs) on the decomposition of residual chlorine. Gas NB injection in treated wastewater improves its properties. The kinetics of disinfectant decay could be impacted by changes in treated wastewater properties. This paper studies the effect of various NB injections on the residual chlorine decay of secondary treated wastewater (STWW). It also outlines the empirical equations that were developed to represent these impacts. The results show that each type of NBs in treated wastewater had a distinct initial chlorine concentration. The outcomes demonstrated a clear impact on the decrease of the needed chlorine quantity and the reduction of chlorine decay rate when utilizing NB injection for the STWW. As a result, the residual chlorine will remain for a longer time and will resist any microbiological growth under the application of NBs on treated wastewater. Moreover, NBs in secondary treated effluent reduce chlorine usage, lowering wastewater disinfection costs.

Unveiling the inherent properties and impact of ultrafine nanobubbles in polar and alcoholic media through unsupervised machine learning and atomic insight

The presence of dissolved gas in the host medium in various industrial processes, such as the presence of oxygen and hydrogen during water splitting or carbon dioxide in fuel cells, or the dissolution of nitrogen for industrial applications, increases the probability of the formation of bubbles at nano-scale. Nanobubbles (NBs), spanning tens to hundreds of nanometres, display exceptional attributes, including remarkable stability, enduring longevity, and an impressive surface-to-volume ratio. These qualities firmly position NBs as pivotal entities with applications that extend across diverse industries, encompassing fields such as energy and chemistry. Therefore, it is crucial to have a comprehensive understanding of their inherent properties and behaviour right from the nucleation stage to grasp their distinctive traits fully. This paper focuses on the combination of data-driven and molecular dynamics simulation to provide a clear understanding of the mechanisms behind NBs nucleation and behaviour in liquids and identify their characteristics. The nucleation process of four gases in two potential liquids for hosting NBs, namely water and methanol, was investigated through scattering nitrogen, oxygen, carbon dioxide, and hydrogen in host liquids using molecular dynamics simulations. Then, high-throughput screening based on the DBSCA (density-based spatial clustering of applications with noise) algorithm was used to screen the formed NB clusters through dissolved gases in the host liquids to determine the size of formed NBs, their density, and motion over time. The findings offer unique insights, indicating that the density of the surrounding liquids is altered and decreased by formed nanobubbles, influenced by the establishment of a nanolayer. The lowest density was recorded for hydrogen and nitrogen nanobubbled methanol samples at 0.61673 and 0.68918 g/ml, respectively, and for hydrogen and nitrogen nanobubbled water samples at 0.93797 and 0.93722 g/ml, respectively. Additionally, the highest density among the scattered gases was observed in non-nanobubbled carbon dioxide methanol and water samples at 0.77724 and 0.99588 g/ml, respectively. Furthermore, the viscosity of the host liquids is influenced, particularly in the presence of high-density nanobubbles, as the highest viscosity was observed for nitrogen nanobubbled water samples at 0.00103 Pa.s.

Transport and retention of nanobubbles in saturated porous media: Overlooked role of grain size and shape

Nanobubbles (NBs) are widely used in groundwater pollution treatment due to their unique properties such as small size and high gas mass transfer rate. Therefore, the transport behavior of NBs in the groundwater environment has attracted more attention. Due to the diversity and complexity of aquifer media in the natural environment, grain sands with different sizes and shapes are ubiquitous. Thus, consideration of grain size and shape in porous media is crucial to predict and evaluate the transport and fate of NBs in soil and groundwater systems. This work investigated the stability of NBs in pure water. The transport behavior and mechanism of NBs under different grain sizes and shapes were demonstrated by the column experiment, microscopic observation, and numerical simulation. The results showed that NBs had excellent stability, remaining in solution for more than 10 days. The retention of NBs in coarse, medium and fine sands was 23.5 %, 43.8 % and 50.8 %, respectively. The retention in sphere sands, oval sands and irregular sands was 22.9 %, 24.0 % and 43.8 %, respectively. The smaller grain size and roundness limited the NBs transport in the saturated porous media, which was mainly due to the complexity of the flow channel and the narrowing of the pore throat of fine sands and angular shape of irregular sand that would hinder the NBs transport. This was proved by the Derjaguin − Landau − Verwey − Overbeek (DLVO) theory and ultra − depth − of − field microscope. The BTCs of NBs were fitted by the one − site deposition model (only attachment) and two − site deposition model (attachment and straining), and it was found that two − site deposition model showed a better fit. The straining rate (kstr) was greater than the attachment rate (katt) in different grain sizes and shapes media, indicating that the retention mechanism of NBs in porous media was a synergistic mechanism dominated by pore straining and supplemented by electrostatic attraction.

Nanobubbles improve peroxymonosulfate-based advanced oxidation: High efficiency, low toxicity/cost, and novel collaborative mechanism

Cl- activated peroxymonosulfate (PMS) oxidation technology can effectively degrade pollutants, but the generation of chlorinated disinfection byproducts (DBPs) limits the application of this technology in water treatment. In this study, a method of nanobubbles (NBs) synergistic Cl-/PMS system was designed to try to improve this technology. The results showed the synergistic effects of NBs/Cl-/PMS were significant and universal while its upgrade rate was from 12.89% to 34.97%. Moreover, the synergistic effects can be further improved by increasing the concentration and Zeta potential of NBs. The main synergistic effects of NBs/Cl-/PMS system were due to the electrostatic attraction of negatively charged NBs to Na+ from NaCl, K+ from PMS, and H+ from phenol, which acted as a "bridge" between Cl- and HSO5- as well as phenol and Cl-/HSO5-, increasing active substance concentration. In addition, the addition of NBs completely changed the oxidation system of Cl-/PMS from one that increases environmental toxicity to one that reduces it. The reason was that the electrostatic attraction of NBs changed the active sites and degradation pathway of phenol, greatly reducing the production of highly toxic DBPs. This study developed a novel environmentally friendly oxidation technology, which provides an effective strategy to reduce the generation of DBPs in the Cl-/PMS system.

The effect of nanobubbles on Langmuir-Blodgett films

Nanobubbles (NBs) are classified in two distinct categories: surface and bulk. Surface NBs are readily observed using atomic force microscopy (AFM), while the existence of bulk NBs has been a subject of debate, conflicting with the diffusion theory's predictions. Current methodologies for identifying bulk NBs yield inconclusive results. In this study, Langmuir Blodgett (LB) technique and AFM, are utilized to visualize NB imprints on anionic, cationic and zwitterionic lipid films deposited on glass-slide substrates. Our analysis of Langmuir monolayers compression isotherms reveals the impact of bulk NBs on lipid monolayer development. AFM scans of the deposited lipid films consistently show NB imprints. Notably, cationic and zwitterionic film depositions exhibit NB formations from the 1st layer, whereas in anionic films, these formations are observed only after the 3rd layer. These results suggest that the origin of these imprinted formations may be attributed to bulk NBs.

Effect of Bulk Nanobubbles on the Flocculation and Filtration Characteristics of Kaolin Using Cationic Polyacrylamide

This study investigated the influence of bulk nanobubbles (NBs) on the flocculation and filtration behavior of kaolin suspensions treated with cationic polyacrylamide (CPAM). Traditionally, flocculation relies on bridging mechanisms by polymers like CPAM. The present work examines the possibility of combining NBs with CPAM to achieve more efficient kaolin separation. The settling behavior of kaolin suspensions with and without bulk nanobubbles was compared. The results with 2 mL CPAM and 300 s settling time revealed that bulk NBs significantly enhanced flocculation efficiency, with supernatant zone height reductions exceeding 50% compared to CPAM alone, indicating a faster settling rate resulting from bulk NBs. This improvement in the settling rate is attributed to NBs’ ability to reduce inter-particle repulsion (as evidenced by a shift in zeta potential from −20 mV to −10 mV) and bridge kaolin particles, complementing the action of CPAM. Additionally, the study demonstrated that bulk NBs improved dewatering characteristics by lowering the medium resistance and specific cake resistance during filtration. These findings pave the way for the utilization of bulk NBs as a novel and efficient strategy for kaolin separation in mineral processing, potentially leading to reduced processing times and lower operational costs.

Enhancing Tetracycline Removal Efficiency through Ozone Micro–Nano Bubbles: Environmental Implication and Degradation Pathway

Enhancing Tetracycline Removal Efficiency through Ozone Micro–Nano Bubbles: Environmental Implication and Degradation Pathway

Nanobubble-assisted liquid phase exfoliation of graphene in deionized water

In this study, we propose a novel method utilizing nanobubbles (NBs) to assist in liquid phase exfoliation. The size, thickness, and number of layers of the resulting graphene solution were characterized and quantified. A highly concentrated graphene solution was achieved through ultrasonic processing in deionized (DI) water, without the need for any surfactant. The observed increase in yield can be attributed to the heightened cavitation within the NBs solution. This study presents a sustainable and cost-effective approach for the large-scale production of graphene, aligning with green and eco-friendly principles.

A dual-targeting therapeutic nanobubble for imaging-guided atherosclerosis treatment

Atherosclerosis is a cardiovascular disease that seriously endangers human health. Low shear stress (LSS) is recognized as a vital factor in causing chronic inflammatory and further inducing the occurrence and development of atherosclerosis. Targeting imaging and treatment are of substantial significance for the diagnosis and therapy of atherosclerosis. On this ground, a kind of ultrasound (US) imaging-guided therapeutic polymer nanobubbles (NBs) with dual targeting of magnetism and antibody was rationally designed and constructed for the efficiently treating LSS-mediated atherosclerosis. Under the combined targeting effect of an external magnetic field and antibodies, the drug-loaded therapeutic NBs can be effectively accumulated in the inflammatory area caused by LSS. Upon US irradiation, the NBs can be selectively disrupted, leading to the rapid release of the loaded drugs at the targeted site. Notably, the US irradiation generates a cavitation effect that induces repairable micro gaps in nearby cells, thereby enhancing the uptake of released drugs and further improving the therapeutic effect. The prominent US imaging, efficient anti-inflammatory effect and treatment outcome of LSS-mediated atherosclerosis had been verified in vivo on a surgically constructed LSS-atherosclerosis animal model. This work showcased the potential of the designed NBs with multifunctionality for in vivo imaging, dual-targeting, and drug delivery in the treatment of atherosclerosis.

Effects of Nanobubbles on Photochemical Processes of Levofloxacin Photosensitizer.

Photodynamic therapy (PDT) stands as an efficacious modality for the treatment of cancer and various diseases, in which optimization of the electron transfer and augmentation of the production of lethal reactive oxygen species (ROS) represent pivotal challenges to enhance its therapeutic efficacy. Empirical investigations have established that the spontaneous initiation of redox reactions associated with electron transfer is feasible and is located in the gas-liquid interfaces. Meanwhile, nanobubbles (NBs) are emerging as entities capable of furnishing a plethora of such interfaces, attributed to their stability and large surface/volume ratio in bulk water. Thus, NBs provide a chance to expedite the electron-transfer kinetics within the context of PDT in an ambient environment. In this paper, we present a pioneering exploration into the impact of nitrogen nanobubbles (N2-NBs) on the electron transfer of the photosensitizer levofloxacin (LEV). Transient absorption spectra and time-resolved decay spectra, as determined through laser flash photolysis, unequivocally reveal that N2-NBs exhibit a mitigating effect on the decay of the LEV excitation triplet state, thereby facilitating subsequent processes. Of paramount significance is the observation that the presence of N2-NBs markedly accelerates the electron transfer of LEV, albeit with a marginal inhibitory influence on its energy-transfer reaction. This observation is corroborated through absorbance measurements and offers compelling evidence substantiating the role of NBs in expediting electron transfer within the ambit of PDT. The mechanism elucidated herein sheds light on how N2-NBs intricately influence both electron-transfer and energy-transfer reactions in the photosensitizer LEV. These findings not only contribute to a nuanced understanding of the underlying processes but also furnish novel insights that may inform the application of NBs in the realm of photodynamic therapy.

A Systematic Study on Long-acting Nanobubbles: Current Advancement and Prospects on Theranostic Properties.

Delivery of diagnostic drugs via nanobubbles (NBs) has shown to be an emerging field of study. Due to their small size, NBs may more easily travel through constricted blood vessels and precisely target certain bodily parts. NB is considered the major treatment for cancer treatment and other diseases which are difficult to diagnose. The field of NBs is dynamic and continues to grow as researchers discover new properties and seek practical applications in various fields. The predominant usage of NBs in novel drug delivery is to enhance the bioavailability, and controlled drug release along with imaging properties NBs are important because they may change interfacial characteristics including surface force, lubrication, and absorption. The quick diffusion of gas into the water was caused by a hypothetical film that was stimulated and punctured by a strong acting force at the gas/water contact of the bubble. In this article, various prominent aspects of NBs have been discussed, along with the long-acting nature, and the theranostical aspect which elucidates the potential marketed drugs along with clinical trial products. The article also covers quality by design aspects, different production techniques that enable method-specific therapeutic applications, increasing the floating time of the bubble, and refining its properties to enhance the prepared NB's quality. NB containing both analysis and curing properties makes it special from other nano-carriers. This work includes all the possible methods of preparing NB, its application, all marketed drugs, and products in clinical trials.

Rare-earth-based polymeric nanobubbles as tri-modal imaging contrast agent

Medical imaging plays an important role in providing sufficient guidance for disease diagnosis and treatment. Among them, multimodal contrast agents can combine with a variety of imaging technologies to obtain comprehensive information on the lesion. It is an important development direction of medical imaging for accurate diagnosis of most diseases. In this study, we have designed and prepared a novel nanocomposite (i.e. NaGdF4:Eu@PLGA NBs) by combining sulfur hexafluoride (SF6) loaded polylactic acid-co-glycolic acid (PLGA) nanobubbles (NBs) with NaGdF4:Eu nanoparticles (NPs), which is the first research work on the composite assembly of ultrasound contrast bubbles with rare earth nanomaterials. Results show that NaGdF4:Eu@PLGA NBs with a diameter of appropriately 220 nm had uniform size and good in vitro stability. Combined with PLGA NBs’ superior ultrasound (US) imaging characteristics and NaGdF4:Eu NPs’ excellent magnetic resonance imaging (MRI) and computed tomography (CT) imaging characteristics, NaGdF4:Eu@PLGA NBs have exhibited significant and long-term in vitro US imaging, MRI and CT tri-modal contrast enhancement capability. Taken together, NaGdF4:Eu@PLGA NBs have great potential to be utilized in biomedical applications of US/MRI/CT tri-modal imaging.

Targeted Ultrasound Nanobubbles Therapy for Prostate Cancer via Immuno-Sonodynamic Effect.

Prostate cancer (PCa) poses a significant global health threaten. Immunotherapy has emerged as a novel strategy to augment the inhibition of tumor proliferation. However, the sole use of anti-PD-L1 Ab for PCa has not yielded improvements, mirroring outcomes observed in other tumor types. This study employed the thin film hydration method to develop lipid nanobubbles (NBs) encapsulating chlorin e6 (Ce6) and anti-PD-L1 Ab (Ce6@aPD-L1 NBs). Our experimental approach included cellular assays and mouse immunization, providing a comprehensive evaluation of Ce6@aPD-L1 NBs' impact. The Ce6@aPD-L1 NBs effectively induced reactive oxygen species generation, leading to tumor cells death. In mice, they demonstrated a remarkable enhancement of immune responses compared to control groups. These immune responses encompassed immunogenic cell death induced by sonodynamic therapy and PD-1/PD-L1 blockade, activating dendritic cells maturation and effectively stimulating CD8+T cells. Ce6@aPD-L1 NBs facilitate tumor-targeted delivery, activating anti-tumor effects through direct sonodynamic therapy action and immune system reactivation in the tumor microenvironment. Ce6@aPD-L1 NBs exhibit substantial potential for achieving synergistic anti-cancer effects in PCa.

Possible physical mechanisms of the high echogenicity of lipid coated nanobubbles

Lipid coated nanobubbles (NBs) have attracted a great level of interest as ultrasound (US) contrast agents due to their ability to extravagate through leaky tumor vasculature. Their linear resonance frequency is in the range of ∼50 M Hz–200 MHz, leading to confusion over their observed strong contrast in diagnostic US frequencies. By solving the Marmottant model, the dynamics of uncoated and lipid coated NBs and microbubbles (MBs) are studied over the frequency and pressure ranges (6–12 MHz, 0.1–1.2 MPa) generally used in diagnostic US. A novel bifurcation analysis in tandem with the analysis of the frequency component of the scattered pressure are conducted. Results show that despite the increased linear resonance frequency and viscous damping due to the lipid shell, buckling and rupture of the shell enhances the generation of the 2nd and 3rd harmonic resonances at pressures as low as 0.2 MPa, not observed with uncoated NBs. The generation of the harmonic resonances are concomitant with an abrupt increase in the 2nd and 3rd harmonic frequency component of the scattered pressure with their pressure threshold (PT) increasing with decreasing NBs size. For the same gas volume, and above the PT, the maximum non-destructive 2nd and 3rd harmonic powers of NBs can become higher than the 2–4 μm MBs. Similar to the lower subharmonic pressure threshold of MBs, the dynamic variation of the NBs effective surface tension due to buckling and rupture may be the potential reason behind the observed harmonic echogenicity.

Size-dependent coalescence of nanobubbles in pure water

Previously, we experimentally demonstrated that similar-sized bulk nanobubbles (NBs) preferentially coalesce when the NB size is ∼100 nm. To explain this peculiar NB coalescence phenomenon, we performed a nanoparticle trajectory analysis to determine the size distribution profile and time-dependent concentration of the bulk NBs generated by pressurizing a porous alumina thin film with ordered straight nanoholes. Furthermore, we propose a physical model based on the hypothesis that the repulsive energy increases when two NBs establish contact, and that coalescence occurs when the kinetic energy of the NBs overcomes the repulsive energy. We simulated the observed NB-size distribution by using the proposed model. The simulation results explained the observed NB-size distribution and lifetime of the NBs. They further indicated that NB coalescence was suppressed for small NBs with diameters of approximately 100 nm or less, resulting in their long lifetimes.

Current status of research on nanobubbles in particle flotation

Froth flotation, as one of the most widely used separation approaches in mineral processing, is commonly used to recover valuable components from minerals. However, maintaining high flotation efficiencies is a serious challenge for conventional froth flotation in the face of decreasing particle size of the minerals to be sorted. To date, there have been plenty of reports on the software of nano-bubbles (NBS) in flotation, and the experimental consequences show that nano-bubbles' introduction has given rise to improvement's different grades in the recovery of varieties of minerals, which highlights the great potential of nano-bubbles for mineral flotation. Nanobubbles have smaller bubble radii and unusually high stability compared to conventional flotation bubbles, and their related behavior in flotation has been a hot research topic. This paper reviews some of the methods of preparing nanobubbles, equipment techniques for characterizing nanobubbles, factors affecting their stability, and some of the popular doctrines. In particular, the reinforcing mechanism of nanobubbles in the particle flotation process is discussed, first, the nanobubbles improve the electrostatic attractiveness with the particles by achieving the charge inversion while the nanobubbles that was adsorbed on the particles' surface will cover a share of the charge, which decreases the electrostatic repulsive force between the particles; and second, the nanobubbles can act as a bridge between the surfaces of the two particles, which advances the agglomeration between the particles. This review aims to be able to further advance the research related to the industrialization of nanobubbles.

Radical generation and bactericidal activity of nanobubbles produced by ultrasonic irradiation of carbonated water

Our previous study showed that nanobubbles (NBs) encapsulating CO2 gas have bactericidal activity due to reactive oxygen species (ROS) (Yamaguchi et al., 2020). Here, we report that bulk NBs encapsulating CO2 can be efficiently generated by ultrasonically irradiating carbonated water using a piezoelectric transducer with a frequency of 1.7 MHz. The generated NBs were less than 100 nm in size and had a lifetime of 500 h. Furthermore, generation of ROS in the NB suspension was investigated using electron spin resonance spectroscopy and fluorescence spectrometry. The main ROS was found to be the hydroxyl radical, which is consistent with our previous observations. The bactericidal activity lasted for at least one week. Furthermore, a mist generated by atomizing the NB suspension with ultrasonic waves was confirmed to have the same bactericidal activity as the suspension itself. We believe that the strong, persistent bactericidal activity and radical generation phenomenon are unique to NBs produced by ultrasonic irradiation of carbonated water. We propose that entrapped CO2 molecules strongly interact with water at the NB interface to weaken the interface, and high-pressure CO2 gas erupts from this weakened interface to generate ROS with bactericidal activity.

Irrigation of Sand-based Creeping Bentgrass Putting Greens with Nanobubble-oxygenated Water

Cultural and environmental factors can place creeping bentgrass (Agrostis stolonifera) under extreme stress during the summer months. This stress, coupled with the growth adaptation of creeping bentgrass, can result in shallow, poorly rooted stands of turf. To enhance root zone oxygen and rooting of creeping bentgrass, golf courses use methods such as core and solid-tine aerification, and sand topdressing. An additional method of delivering oxygen to the soil could be irrigation with nanobubble-oxygenated water. The properties of nanobubbles (NBs) allow for high gas dissolution rates in water. Irrigating with NB-oxygenated water sources may promote increased rooting of creeping bentgrass putting greens during high-temperature periods and lead to a more resilient playing surface. The objectives of this study include comparing the effects of irrigation with NB-oxygenated water sources with untreated water sources on creeping bentgrass putting green root zone and plant health characteristics using field and controlled environment experiments. Treatments included NB-oxygenated potable water and irrigation pond water, and untreated potable and irrigation pond water. In the field, NB-oxygenated water did not enhance plant health characteristics of creeping bentgrass. In 1 year, NB-oxygenated water increased the daily mean partial pressure of soil oxygen from 17.48 kPa to 18.21 kPa but soil oxygen was unaffected in the other 2 years of the trial. Subsurface irrigation with NB-oxygenated water did not affect measured plant health characteristics in the greenhouse. NB-oxygenation of irrigation water remains an excellent means of efficiently oxygenating large volumes of water. However, plant health benefits from NB-oxygenated irrigation water were not observed in this research.