Good quality single-photon emission computed tomography (SPECT) images are required to achieve a perfect diagnosis and determine the severity of defects within the myocardial wall. There are many techniques that can support the diagnosis of defect formations in acquired images and contribute to avoiding errors before image construction. The main aim of this study was to determine the effect of energy width (15%, 20%, and 25%) on defect contrast in myocardial SPECT images correlated with the decentralization of positioning of a phantom. A phantom of polyethylene plastic was used to mimic the myocardial wall of the left ventricle. The phantom consists of two chambers, inner and outer. Two rectangular pieces of plastic were placed in anterior and inferior locations in the mid-region of the myocardial phantom to simulate myocardial infarction (defects). The average defect contrast for all phantom positions using 15% to 20% energy was (1.2, 1.6) for the anterior region and (1.1, 2) for the inferior region, respectively. Additionally, the energy window width was >25% with a large displacement of the positioning off center, leading to loss of the defect contrast in myocardial SPECT images, particularly in the inferior region. The study showed decreasing defect contrast in both locations, anterior and inferior, with increasing energy window width correlated with eccentricity positioning of the phantom on an imaging table.