Objective. To evaluate myocardial function in patients with chronic heart failure with preserved left ventricular ejection fraction (CHF-PEF) by speckle tracking echocardiography. Design and methods . We examined 56 patients aged from 50 to 69 years with verified CHF-PEF, NYHA (New York Heart Association) class I–IIa and stage A–C of the ABCD classification of the American College of Cardiology, and 15 healthy persons. CHF-PEF was associated with arterial hypertension and coronary artery disease. The results of 6-minute walk test, echocardiography and speckle-tracking echocardiography were analyzed. Results. The average left ventricular ejection fraction measured by Simpson’s method was 61,7 ± 5,28 %. According to the 6-minute walk test the patients were divided as followed: 26,7 % — stage A, 48,2 % — stage B, 25,1 % — stage C. All patients with CHF-PEF had the diastolic dysfunction: in 48 patients abnormal relaxation pattern was found, and in 8 pseudonormal pattern was shown. Patients with CHF-PEF had reduced global longitudinal strain (GLS –16,6 ± 2,58 %) and GLS rate (GLSR –0,74 ± 0,12 s) of the left ventricle. Also they had reduced strain and strain rate in basal anteroseptal (–13,2 ± 3,24 % and –0,75 ± 0,03 s-1) and basal anterolateral (–14,16 ± 3,29 % and –0,83 ± 0,12 s-1) segments. Conclusions. Patients with CHF-PEF have abnormal relaxation pattern and pseudonormal pattern of the diastolic dysfunction. They also have reduced global and segmental strain and strain rate of the left ventricle.