Double-transition-metal (DTM) MXenes are potentially multifunctional materials with greater compositional and structural tunability than mono-transition-metal (MTM) MXenes, enabling controllable formation of defects and controllable tuning of material properties. Herein, we investigate Ti2VC2Tx's ammonia gas sensing via experiments and theoretical calculations. Ti2VC2Tx exhibited a notable response, exceeding 25 %, to 100 ppm ammonia at room temperature with rapid response/recovery times of 4 s/16.2 s. Furthermore, the Ti2VC2Tx sensor shows exceptional ammonia selectivity, attributed to closer adsorption distances and higher adsorption energies of ammonia molecules. This study broadens the application area of DTM MXenes and further demonstrates the potential of MXene materials for gas sensing.