The natural conducting Mimosa pudica L. fiber was extracted from the stem of the herb by sinking the stem in 15% NaOH solution for 1 week. The processed fiber was irradiated with 6 MV X-ray photon beams with a dose of 20 Gy using a medical linear accelerator. X-ray of energy (60–100 keV) one order or more than the usual X-ray energy is known as the high energy. The effect on the fiber structure and its orientation due to irradiation were investigated using X-ray diffraction technique. Full width at half maxima of the diffraction profile turned out to be decreased with fiber orientation. Increase of dynamic current density inside the irradiated fiber found to be well in agreement with X-ray results.