Muticum Extract Research Articles

Apo-9'-fucoxanthinone, isolated from Sargassum muticum, inhibits CpG-induced inflammatory response by attenuating the mitogen-activated protein kinase pathway.

Sargassum muticum (S. muticum) is a brown edible alga and widely distributed in Korea. This report was designed to evaluate the anti-inflammatory properties of apo-9′-fucoxanthinone (APO-9′) isolated from S. muticum on pro-inflammatory cytokine production. S. muticum extract (SME) exhibited significant inhibitory effects on pro-inflammatory cytokine production in bone marrow-derived macrophages (BMDMs) and dendritic cells (BMDCs). APO-9′ pre-treatment in the CpG DNA-stimulated BMDMs and BMDCs showed a strong dose-dependent inhibitory effect on interleukin (IL)-12 p40, IL-6 and tumor necrosis factor (TNF)-α production with IC50 values ranging from 5.31 to 13.79. It exhibited a strong inhibitory effect on the phosphorylation of ERK1/2 and on activator protein (AP)-1 reporter activity. APO-9′ pre-treatment exhibited significant inhibition of CpG DNA-induced production of inducible nitric oxide synthase. Taken together, these data suggest that SME and APO-9′ have a significant anti-inflammatory property and warrant further studies concerning the potentials of SME and APO-9′ for medicinal use.

Brown alga Sargassum muticum inhibits proinflammatory cytokines, iNOS, and COX-2 expression in macrophage RAW 264.7 cells

The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH_2Cl_2, EtOAc, BuOH, and water. The results indicate that the CH_2Cl_2 fraction of S. muticum extract was an effective inhibitor of LPS-induced NO and PGE_2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects of S. muticum fractions on other cytokines, we also measured IL-1β and IL-6 mRNA expression by RT-PCR in LPS-stimulated RAW 264.7 macrophage cells. In these assays, the CH_2Cl_2 fraction of S. muticum decreased the expression of IL-1β and IL-6 mRNA in a dose-dependent manner. Based on these results, we suggest that S. muticum extracts may be considered possible anti-inflammatory candidates for human health.

경단구슬모자반(Sargassum muticum) 추출물의 항산화 및 항균활성

본 연구는 갈조류인 모자반속 중에서 경단구슬모자반(Sargassum muticum)을 대상으로 에탄올추출 및 용매분획하여 항산화활성 및 항균활성을 비교 분석하였으며, 아울러 총 폴리페놀 및 플라보노이드 함량 변화를 측정하여 그 상관성 등을 조사함으로써 식품 저장성이나 안전성을 향상하기 위한 식품첨가제나 보존제로서의 개발 가능성을 알아보고자 하였다. 그 결과 경단구슬모자반 에탄올 추출물 및 순차적 분획물의 항산화활성은 처리 농도가 높아질수록 항산화 활성도 비례해서 증가되었으며, 특히 순차적 용매분획물 중 디클로로메탄과 에틸아세테이트 분획물에서 항산화활성이 가장 우수하게 나타났다. 또한 항균활성은 순차적 용매분획물 중에 디클로로메탄 분획물이 본 실험에 사용된 6종의 균주 모두에서 가장 효율적인 항균활성을 보여 주었다. 이러한 경단구슬모자반 에탄올 추출물과 순차적 분획물별 항산화 및 항균활성 결과는 총 폴리페놀 및 플라보노이드의 함량과 유의한 양의 상관관계를 나타내었으며, 한편 총 폴리페놀 함량은 에틸아세테이트 분획물＞디클로로메탄 분획물＞부탄올 분획물＞헥산 분획물＞에탄올 추출물＞수용성 분획물 순으로, 총 플라보노이드 함량은 에틸아세테이트 분획물＞디클로로메탄 분획물＞헥산 분획물＞부탄올 분획물＞에탄올추출물＞수용성 분획물 순으로 나타났다.