Mustard Plants Research Articles

Phenylalanine supply alleviates the drought stress in mustard (Brassica campestris) by modulating plant growth, photosynthesis, and antioxidant defense system

Mustard (Brassica campestris L.) is a major oilseed crop that plays a crucial role in agriculture. Nevertheless, a number of abiotic factors, drought in particular, significantly reduce its production. Phenylalanine (PA) is a significant and efficacious amino acid in alleviating the adverse impacts of abiotic stressors, such as drought. Thus, the current experiment aimed to evaluate the effects of PA application (0 and 100 mg/L) on brassica varieties i.e., Faisal (V1) and Rachna (V2) under drought stress (50% field capacity). Drought stress reduced the shoot length (18 and 17%), root length (12.1 and 12.3%), total chlorophyll contents (47 and 45%), and biological yield (21 and 26%) of both varieties (V1 and V2), respectively. Foliar application of PA helped overcome drought-induced losses and enhanced shoot length (20 and 21%), total chlorophyll contents (46 and 58%), and biological yield (19 and 22%), whereas reducing the oxidative activities of H2O2 (18 and 19%), MDA concentration (21 and 24%), and electrolyte leakage (19 and 21%) in both varieties (V1 and V2). Antioxidant activities, i.e., CAT, SOD, and POD, were further enhanced under PA treatment by 25, 11, and 14% in V1 and 31, 17, and 24% in V2. Overall findings suggest that exogenous PA treatment reduced the drought-induced oxidative damage and improved the yield, and ionic contents of mustard plants grown in pots. It should be emphasized, however, that studies examining the impacts of PA on open-field-grown brassica crops are still in their early stages, thus more work is needed in this area.

Penggunaan Teknologi Tepat Guna Sistem Hidroponik dengan Memanfaatkan Lahan Kosong Di Kelurahan Rembang

One of the problems that existed at the Rembang Village Office was due to the vacant land which was one of the obstacles, the UPNVJ Thematic Community Service students decided to use hydroponics as a planting medium to be developed because it did not require large areas of land, as well as the tools and materials needed to make hydroponics. easy to get and how to make it easy. Mustard greens are one of the easiest plants to grow using hydroponic growing media, so we decided to plant mustard greens because of its ease of cultivation. Compared to existing planting media, hydroponic growing media has better possibilities. So that the planting medium only needs to be fertilized, watered, and occasionally cleaned. In order for the Rembang Village Office to become an example of hydroponic planting for Rembang Village residents, UPNVJ Thematic KKN students established and planted using hydroponic planting materials to optimize this.

PEMBERIAN PUPUK ORGANIK CAIR LIMBAH TAHU DALAM MEDIA HIDROPONIK RAKIT APUNG TERHADAP PERTUMBUHAN TANAMAN SAWI HIJAU (Brassica juncea L.)

The content of protein, fiber, vitamins, and fat contained in mustard greens (B. juncea) is the main attraction for people to consume vegetables. Green mustard plant growth using hydroponic media can help optimize the absorption of nutrients needed by plants. The application of the floating raft hydroponic method with the addition of liquid organic fertilizer from tofu liquid waste is an effort that is expected to overcome this problem. This research to determine the impact and concentration of POC tofu liquid waste which has the best impact on the growth of green mustard plants. Use of RAL with 6 levels of treatment, namely P1=0%POC; P2 = 35%POC; P3 = 40%POC; P4 = 45%POC; P5 = 50%POC; P6 = 55%POC. Data were analyzed using ANOVA with a 95% confidence level. The application of liquid organic fertilizer significant impact on measuring plant height, number of leaf blades, leaf surface area, root shoot wet weight, root shoot dry weight, and chlorophyll. The best liquid organic fertilizer treatment of tofu waste was the concentration of 55% for plant height (31.72 cm), number of leaves (13.50), leaf area (1287 mm), crown wet weight (65.95 g), wet weight root (2.97 g), shoot dry weight (37.8 g), root dry weight (1.25 g), chlorophyll a content (24.74 mg/g), b (30.54 mg/g) and total (52.40 mg/g).

TPC1 vacuole SV channel gains further shape - voltage priming of calcium-dependent gating.

Across phyla, voltage-gated ion channels (VGICs) allow excitability. The vacuolar two-pore channel AtTPC1 from the tiny mustard plant Arabidopsis thaliana has emerged as a paradigm for deciphering the role of voltage and calcium signals in membrane excitation. Among the numerous experimentally determined structures of VGICs, AtTPC1 was the first to be revealed in a closed and resting state, fueling speculation about structural rearrangements during channel activation. Two independent reports on the structure of a partially opened AtTPC1 channel protein have led to working models that offer promising insights into the molecular switches associated with the gating process. We review new structure-function models and also discuss the evolutionary impact of two-pore channels (TPCs) on K+ homeostasis and vacuolar excitability.

PENGARUH PEMBERIAN BIOCHAR DAN PUPUK KANDANG AYAM TERHADAP KADAR AIR DAN BEBERAPA SIFAT KIMIA TANAH PESISIR SERTA PERTUMBUHAN TANAMAN SAWI

Southeast Sulawesi has a coastline length of 1,740 km, but the community has not made optimal use of the coastal land, especially for agricultural activities. The purpose of this study is to determine the effect of applying biochar and chicken manure on water content and some chemical properties of coastal soils, to determine the effect of biochar and chicken manure interaction on the growth of mustard plants on coastal soils and find out better doses of biochar and chicken manure on the growth of mustard plants. This study used a Randomized Block Design (RBD) with a factorial pattern consists of two factors, namely biochar B0 = 0 g polybag-1, B1 = 75 g polybag-1 equivalent to 30 t ha-1, B2 = 150 g polybag-1 equivalent to 60 t ha-1 and chicken manure which is P0 = 0 g polybag-1, P1 = 75 g polybag-1 equivalent to 30 t ha-1, P2 = 150 g of polybag-1 is equivalent to 60 t ha-1. Observed variables of moisture content, some chemical properties of the soil as well as plant height and number of leaves. The results showed that the application of biochar and chicken manure was able to increase the air content, pH, Organic-C and total-N. In the B1P1 treatment, the interaction effect on the height and number of mustard leaves and the dose of fertilizer that gave a better effect than other treatments, namely biochar 75 g polybag-1 or equivalent to 30 t ha-1 + chicken manure 75 g polybag-1 or equivalent to 30 t ha-1

Pengaruh Komposisi Media Tanam Organik Terhadap Hasil Tanaman Sawi (Brassica juncea L.)

The purpose of the study was for various compositions of growing media on the yield of mustard plants (Brassica juncea L.). The method used in this study was a complete randomized design (CRD) with 6 treatments and each treatment was repeated 5 times. The treatment made is K0: Soil Media only; K1: Soil Media + Compost (2 : 1); K2 : Soil Media + Compost + Goat Manure (2 : 1 : 1); K3: Soil Media + Compost + Cocopeat (2 : 1 : 1); K4: Soil Media + Compost + Cow Manure (2 : 1 : 1); K5: Soil Media + Compost + Chicken manure (2 : 1 : 1). The results showed that the treatment of the composition of the planting media tried had the same effect on all observation parameters: plant length, number of leaves, and wet weight of mustard plants. With various media composition treatments tried, mustard plants are able to produce wet weight of mustard plants weighing 25.32 g – 118.5 g / polybag.

Pengaruh Pemberian Pupuk Trichokompos Kotoran Kambing Terhadap Pertumbuhan Sawi Pahit (Brassica juncea L.)

Bitter mustard, known as salty mustard or green mustard, is one of the most popular vegetable commodities and has high economic value. Trichocompost is an alternative to increase the growth of mustard greens. This study aims to determine the appropriate dose of goat kohe trichocompost fertilizer to produce high growth and production of mustard greens. Completely randomized design with four treatments, namely M0 : control, M1 : 100 gr.plant-1, M2 : 120 gr.plant-1 and M3 : 140 gr.plant-1 repeated four times. The results of the study concluded that the application of goat trichocompost kohe fertilizer had a significant effect on plant height, number of leaves, leaf area, fresh weight, and dry weight of bitter mustard plants. The best dosage of goat kohe trichocompost fertilizer that can increase the growth and productivity of bitter mustard plants is a dose of 140 gr.plant-1.

Biofumigation by Mustard Plants as an Application for Controlling Postharvest Gray Mold in Apple Fruits

Gray mold caused by Botrytis cinerea is a critical disease that results in severe postharvest losses for the apple industry. In recent years, biological control has become an increasingly effective approach for controlling postharvest diseases in fruits. Brassica plants contain abundant natural compounds with known antimicrobial activity against numerous plant pathogens. In this study, a large-scale screening of 90 mustard cultivars was conducted to evaluate their biofumigation effects against B. cinerea. Among these, one mustard cultivar named Dilong-1, displayed the highest inhibitory effect against B. cinerea, and was able to completely inhibit mycelial growth. Further investigations showed that fumigation with Dilong-1 inhibited mycelial growth, sporulation, and spore germination of B. cinerea in vitro. In addition, fumigation using Dilong-1 showed a wide antifungal spectrum, including other fruit postharvest pathogens such as Phytophthora litchii. Furthermore, apple gray mold disease severity was significantly reduced by biofumigation using Dilong-1. Importantly, fumigation with Dilong-1 did not negatively impact final apple qualities, including weight loss, firmness, and total soluble solids. These results suggested that Dilong-1 significantly inhibited gray mold decay caused by B. cinerea without affecting the quality of apple fruits. In conclusion, biological fumigation of apple fruits with the mustard cultivar Dilong-1 is a promising eco-friendly approach for controlling apple gray mold during storage and shipment.

Molecular Mechanism of Exogenous Selenium Affecting the Nutritional Quality, Species and Content of Organic Selenium in Mustard

It is an essential method for healthy Selenium (Se) supplementation to convert exogenous Se into organic Se via crops. Brassica juncea (L.) Czern (leaf mustard) was employed as plant material in this investigation and was treated with sodium selenite (Na2SeO3). Its physiological indicators, nutritional quality, antioxidant enzyme activity, total Se content, and Se morphology were all evaluated. The absorption, transportation, and transformation mechanisms of Se in mustard were studied using transcriptome data. The results revealed that low concentration of Se treatment promoted the growth of mustard, while high concentration Se treatment inhibited it. The concentration of 10 mg/L Na2SeO3 treatment had the best growth parameters for mustard. Compared to the control group, the content of vitamin C (Vc) and anthocyanins in the treatment group increased to varying degrees, while the content of flavonoids, total phenols, soluble sugar, and soluble protein increased first and then decreased. Five Se forms, Se (IV), Se (VI), selenocystine(SeCys2), selenomethionine (SeMet), and methylselenocysteine (MeSeCys), were detected in the Na2SeO3 treatment group, with organic Se accounting for over 95%. Na2SeO3 treatment can significantly reduce the accumulation of ROS in mustard plants and enhance their stress resistance. Transcriptome data and metabolite association analysis showed that PHO1-H8 promoted the absorption of Na2SeO3 by mustard roots, while SULTR3;3 and SULTR4;1 promoted the transport of Se from roots to the aboveground portion and chloroplasts. Se in mustard was transformed into SeMet, SeCys, MeSeCys, and selenoprotein through the action of genes such as APS, APR, and SEP1, and stored in plant leaves.

PENGARUH EKSTRAK KUNYIT TERHADAP PERTUMBUHAN TANAMAN SAWI HIJAU DI DESA BINTANG BARU KECAMATAN SUSUA KABUPATEN NIAS SELATAN

Based on the background of the problem, the continuous use of chemical fertilizers can make the soil harden and lose its porosity. In addition, the people in Bintang Baru Village lack understanding of the content of turmeric which can be processed into natural fertilizer for the growth of green mustard plants. This study aims to determine the effect of turmeric extract on the growth of mustard greens. This research uses a type of quantitative research using pure experimental methods (true experiment) and using a completely randomized design trial (CRD). The population of this study was green mustard plants, which totaled 20 polybags of plants with five treatments and four replications. The treatments used were P0 (without turmeric extract), P1 (50 ml), P2 (100 ml), P3 (150 ml) and P4 (200 ml). The results of this research data were analyzed using the SPSS version 20 application which consisted of a normality test, homogeneity test, hypothesis test and follow-up test. Based on the results of this study, the use of turmeric extract can affect the growth of mustard greens, especially in: (1) Increase in the number of leaf blades, (2) Increase in plant stem height. It was concluded that giving turmeric extract to mustard greens is one way to grow naturally and easily available. Suggestions offered by researchers, turmeric extract should be applied and able to improve agricultural quality.

Effect of Biotechnological Fermentation Waste Kombucha Flower Telang (Clitoria ternatea L) as Liquid Fertilizer on The Growth of Sawey (Brassica chinensis var. parachinensis)

Liquid organic fertilizer comes from nature and has the potential to improve biological, physical and soil properties because it contains nutrients that have the potential to increase plant growth. This research was conducted to determine liquid fertilizer made from telang kombucha fermentation waste on the growth of mustard plants. The method used was a completely randomized design with a single factor, namely the difference in the concentration of liquid organic fertilizer given 6 treatments with 5 replications for each treatment. The research data were analyzed statistically through ANOVA at a significance level of 95%. Parameters in this study were plant height, number of leaves, fresh weight and dry weight. The results of this study prove that the spraying treatment of liquid organic fertilizer containing kombucha fermentation waste of butterfly pea flowers has an effect on the growth parameters of mustard plants. The conclusion is that liquid organic fertilizer from the waste of fermented kombucha flower butterfly pea at a concentration of 5 mL/L can increase plant height, number of leaves, wet and dry weight of mustard plants.

The effect of AB mix and organic fertilizer concentration on growth and yield of two mustard varieties in a floating hydroponic system

The Efforts to increase mustard production can be carried out by developing cultivation technics using a Floating Hydroponic System (FHS) with additional nutrients to support plant growth. This study aims to determine the best combination of AB Mix and organic fertilizer nutrient concentrations for the growth and yield of two types of mustard plant varieties using FHS hydroponics. The organic fertilizer used was POC NASA, which contains sufficient macro and micronutrients for plant production. This study was conducted at the Greenhouse in the Faculty of Agriculture, Universitas Syiah Kuala from December 2021 to February 2022. The design used was a 4x2 Split Plot Design with 3 replications. Main plots (Tosaka and Nauli F1 varieties) and sub-plots (5 ml/L AB Mix+2 ml/L POC NASA, 7 ml/L AB Mix+2 ml/L POC NASA, 9 ml/L AB Mix+2 ml/L and 11 ml/L AB Mix+ 2 ml/L POC NASA). The results showed that the best mustard plant growth was found in the combination of 9 ml/L AB Mix + 2 ml/L POC NASA and the Tosaka variety. There is an interaction between the combination of treatment of nutrient concentrations of AB Mix and organic fertilizer with varieties that significantly affect fresh weight per plant. The best mustard plant yields were found in the combination of 9 ml/L AB Mix + 2 ml/L POC NASA nutrient concentration on the Nauli F1 variety.

Ecofriendly Approaches for Controlling Mustard Aphid (Lipaphis erysimi Kalt.) Infestation

Brassica oilseed crops are grown all over the world, but the aphids of cabbage, Brevicoryne brassicae L. and Turnip aphid Lipaphis erysimi Kalt, cause mischief to the crops everywhere in the world. A field experiment was conducted at the Agricultural Farm Area of Airport Campus of Ghazi University Dera Ghazi Khan during mid-November 2019 to test eco-friendly management methods for mustard aphids. The experiment was laid out in a randomized complete block design with three replications and five treatments in 2 m ×2.7 m size plots. Seeds of the Khanpur Raya variety were sown in mid-November 2019. The treatments included (i) Neem leaf extract, (ii) Dimethoate, (iii) Lemongrass oil, (iv) Mustard Cake, and (v) Control (untreated). The overall results of the experiment showed that after the second spray, the aphids became immune to synthetic pesticides. However, bio-pesticides had some control over the aphids, with Lemongrass oil producing the best results, achieving almost 80 percent control, followed by about 72 percent control with neem extract and almost 67 percent control with mustard cake. This suggests that Lemongrass oil can be used to control aphids on mustard plants and, since it is a bio-pesticide, it has many biological benefits. Therefore, it can be concluded that using botanical products for managing mustard aphids can be an effective and eco-friendly approach

Hazardous waste leachates induced changes in plant water relation, photosynthetic pigments, heavy metal accumulation and yield of mustard (Brassica juncea L.) plant

Chromium (Cr) contamination by industrial sites in water and agricultural soils is common and responsible for the human body being at risk from this heavy metal. The present study aims to assess the plant growth, yields, photosynthetic pigments, water relation, and heavy metal accumulation to understand the nature of the adaptation of Cr overload in mustard plants. An experiment was conducted at different levels of Basic Chrome Sulphate (BCS) sludge leachates i.e., T1 (control without leachates), T2 (25% leachates+75% DW); T3 (50% leachates + 50% DW); T4 (75% leachates + 25% DW) and T5 (100% leachates) on mustard (Brassica juncea L.) plant in completely randomized design (CRD). The primary leachates were collected from the dumping site of BCS sludge which was situated at 26⁰24′57′' NL and 80⁰3′28′' EL at Khanchandpur Kharanja, Rania, Kanpur Dehat district of Uttar Pradesh, India. Results indicated that the vegetative growth and yield were significantly decreased on increasing the dose of leachates. The linear regression coefficient (R2) values showed that the number of seeds, biomass, and seed yields per plant were more significantly affected (R2 = 0.963, R2 = 0.955, and R2 = 0.872 respectively) than vegetative growth (R2 = 0.706). The photosynthetic pigments like chlorophyll `a' (Chl. a), chlorophyll `b' (Chl. b), and carotenoid contents were significantly increased on increasing the dose of leachates while the Chl.`a'/Chl.`b' ratio decreased but carotenoid/ chlorophyll ratio increases. The leaf surface area and relative water contents (RWC) were significantly decreased while the degree of succulence and specific water content were significantly increased. Maximum Cr concentration was observed in the root up to T4 levels and further sharply declined while shoot and seed, it was not detected up to T4 but further sharply increased which may be due to Cr being restricted in the root up to a certain limit of Cr load in mustard plant and further on increasing the load it enters into the shoot and affects to growth and yields. The accumulation of Cr, Ni, and Pb was increased on increasing leachate dose because leachates also contain these heavy metals. The results of the Pollution Load Index (PLI) indicated that Cr scored maximum (1.792) pollution load and showed descending order as Cr > Zn >Pb>Cu>Cd>Ni.

The Effectiveness of Using NPK Compound Fertilizer on the Growth and Yield of Mustard Plants (Brassica juncea L.)

This study aims to determine the extent to which the effectiveness of using NPK compound fertilizer on the growth and yield of mustard plants. This study used a simple randomized block design (RBD) consisting of eight treatments with three replications and for observation, each treatment was represented by two sample plants. The placement of the treatments in the experimental plots was carried out randomly, the growth and yield variables of the mustard plants observed in this study were the length of the plants, observed at the age of 10 days, 20 days and 30 days after transplanting, the number of leaves were observed at the ages of 10 days, 20 days and 30 days after transplanting and plant wet weight. This was done when the plants were 30 days after transplanting. While the results of the study showed a significant effect on the observation variables of plant length, number of leaves and fresh weight of mustard plants due to the use of NPK compound fertilizer and effective (effective) doses in the use of NPK compound fertilizer during growth and yield of mustard plants tended to be achieved by treatment with doses of NPK compound fertilizer. 250 kg per hectare, although the highest growth and yield tended to be shown in the treatment of 350 kg of NPK compound fertilizer per hectare, followed by the treatment of 300 kg of NPK compound fertilizer per hectare.

PENGARUH KONSENTRASI BIOURINE SAPI TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN SAWI (Brassica juncea L.)

This study aims to determine the effect of giving various concentrations of cow bio urines on the growth and yield of mustard plants grown on bed media. This research was conducted in Samaulue Village, Lanrisang District, Pinrang Regency, South Sulawesi Province. This study took place from December 2021 to February 2022. The study used a randomized block design consisting of 3 levels of treatment, namely 150 ml/liter, 250 ml/liter, and 300 ml/liter, and treatment without urine. Each treatment was repeated three times to get 12 experimental units. The results showed that using a concentration of 250 ml/liter cow urine fertilizer could increase the growth and yield of mustard plants. The average plant height was 38.98 cm, the wet weight of the plant was 235.67 g, the dry weight of the plant was 23.67 g and the consumption weight was 234.33 g.

The Effect of Biostarter Addition on Kudzu (Pueraria Javanica) Compost to Nutrient Content and N Adsoprtion of Mustard (Brassica Juncea L.)

Compost fertilizer is very influential on the nutrient status of the soil and plants. Giving compost fertilizer will also have a good effect on the growth and development of mustard plants. This study aims to determine the effect of the addition of biostarter on compost fertilizer to N-total and N nutrient absorption on mustard plants. The experiment was structured using factorial randomized group design. The method in this study using compost analysis,soil analysis, and plant analysis. The results showed that the nine samples have different nutrient content and nutrient nutrient absorption resulting in different effects on soil and mustard plants. Nitrogen in the soil greatly affects the N nutrient absorption on plants, the two parameters have a positive correlation which means directly proportional. Nitrogen absorbed by plants affects N-total levels in plants. N levels in plants and soil is very influential on the color of the leaves of mustard plants. Through the nutrient levels can be known proper fertilization recommendations for mustard plants. Based on the analysis of nutrient levels in soil and plants known fertilizer recommendations in nine samples with the addition of biostarter in kudzu compost and fertilizer doses.

Pertumbuhan Dan Produksi Tanaman Sawi Melalui Aplikasi Pupuk Organik Lumpur Laut Dengan Pupuk Kandang

The growth of mustard plants is influenced by the availability of nutrients through the application of fertilizers. Manure and sea mud are one type of biological fertilizer. The study aims to determine the optimal dose of sea mud and manure for the growth and production of mustard plants. The study design was a randomized group design consisting of 2 factors and repeated 3 times. The first of Sea Mud treatment (L) consists of levels, namely: L0 = Control; L1=500 g, L2= 750 g. L3 1000 g. The second factor is that the dose of manure consists of levels, namely: P0=without manure; P1=120 g, P2=160 g: and P3 = 200g. The results showed that L3 treatment was able to increase plant height, while the number of leaves was best in L2 treatment. This treatment was also able to increase the number of leaves, fresh weight and dry weight but between L2 and L3 treatments did not differ markedly. P3 manure treatment is able to increase the best number of leaves, while fresh weight is best P2 treatment. The interaction between treatments only occurred in plant height and leaf area parameters at 17 HST observations.

Biopriming with Bacillus subtilis Enhanced the Sulphur Use Efficiency of Indian Mustard under Graded Levels of Sulphur Fertilization

This study investigated the effect of bioinoculants (Bacillus subtilis and Pseudomonas fluorescens) as biopriming agents under varied sulphur (S) fertilizer levels (0, 20, 30, and 40 kg S ha−1) to enhance sulphur use efficiency (SUE) in Indian mustard. The experiment was conducted during the 2018–19 and 2019–20 winter seasons at the research farm of the Institute of Agricultural Sciences, Banaras Hindu University, Varanasi (25°26′ N, 82°99′ E). A randomized block design was employed to assess the combined effect of biopriming and S fertilization on the partitioning of S in different parts of mustard plants, S uptake, SUE, and soil urease, dehydrogenase, alkaline phosphatase, and arylsulphatase activity. Results showed that the application of S fertilizers along with biopriming significantly increased the S content, uptake, and SUE by plants and enzymes involved in the S mineralization process. Application of 40 kg S ha−1 + B. subtilis resulted in the highest S content in the root (0.12%), stover (0.30%), and seed (0.67%), and the highest total S uptake (2.97 g m−2 in the first year and 3.37 g m−2 in the second year), agronomic use efficiency (8.80 g g−1), apparent S recovery (22.37%), urease activity (156.68 µg NH4+ g−1 hr−1), dehydrogenase activity (42.80 µg TPF g−1 24 hr−1), and arylsulphatase activity (39.94 µg pNP g−1 hr−1). However, the highest alkaline phosphatase activity (129.17 µg pNP g−1 hr−1) was found in the treatment that received 40 kg S ha−1 + P. fluorescens. Further, the different indices of SUE revealed that the effect of biopriming was more prominent in apparent recovery efficiency than agronomic SUE and physiological SUE. Conclusively, the present study demonstrated that seed biopriming with B. subtilis along with S fertilization is more rewarding and can promote sustainable production of Indian mustard.

Exiguobacterium sp. as a bioinoculant for plant-growth promotion and Selenium biofortification strategies in horticultural plants

Plant growth-promoting rhizobacteria (PGPR) have a positive effect on plant development and being a promising way to enhance crop productivity and as substitution of chemical fertilizers. Selenium (Se) is an important trace element and its intake is usually lower than the daily minimum amount required for humans; hence, there is a demand on the design of Se biofortification strategies. Here, the genetic traits known to be associated with Plant-Growth Promotion (PGP) and Se biotransformation of Exiguobacterium sp. S17 were evaluated through genome analysis. Its growth-promoting capacity was tested through plant-growth promotion assays in laboratory and field conditions, using Brassica juncea (indian mustard), Beta vulgaris (chard), and Lactuca sativa (lettuce). Additionally, the Se biotransformation ability of Exiguobacterium sp. S17 was evaluated and the obtained selenized bacteria were tested in mustard plants. The sequenced bacteria genome revealed the presence of multiple genes involved in important functions regarding soil and plant colonization, PGP and Se biotransformation. Moreover, it was demonstrated that Exiguobacterium sp. S17 enhanced plant growth and could be useful to produce Se accumulation and biofortification in accumulator plants such as mustard. Thereby, Exiguobacterium sp. S17 might be used for developing new, sustainable, and environmentally friendly agro-technological strategies.