A field experiment was conducted during the Rabi season of 2022 at the Rajoula Agriculture farm, of Mahatma Gandhi Chitrakoot Gramodaya Vishwavidyalaya Chitrakoot, Satna (M.P.). The present experiment having 13 treatment combinations replicated thrice in a randomized block design. Mustard variety Pusa Mahak was grown with recommended agronomic practices. Based on the results emanated from present investigation, it could be concluded that application of T12 [½ of RDN + (Ist nano-urea spray + 2nd nano-urea spray + 1.25 kg B)] significantly recorded maximum growth parameters such as plant height (198.8 cm), number of branches plant-1 (8.7) and maximum yield attributing characters such as number of siliqua plant-1 (166.8), number of seed siliqua-1 (13.4) and 1000 seed weight (5.1 g). The result showed the highest grain yield (1523.81 kg ha-1) and straw yield (4761.90 kg ha-1), with treatment T12 [½ of RDN + (Ist nano-urea spray + 2nd nano-urea spray + 1.25 kg B)] in comparison to all the treatments. While the minimum value of growth parameters viz. plant height (171.2 cm), number of branches plant-1 (6.3) and yield attributes such as number of siliqua plant-1 (117.5), number of seed siliqua-1 (11.7) and 1000 seed weight (3.8 g) recorded under the treatment T1 [100 % NPK as per RDF].