The focus of this article is to examine the historical foundation of the Holy Qur’an’s manuscript in Malaysia, with specific attention to its calligraphic style and illumination. Islamic calligraphy and manuscript traditions hold significant importance within the Malaysian Muslim heritage. These traditions have been inherited and evolved over generations, with the earliest examples of Arabic calligraphy being found in the Holy Qur'an, commonly inscribed in a script known as Kufic. The historical roots of Islam are deeply intertwined with the Holy Qur'an's manuscript, making it a primary source for understanding the advent of Islam. In this research, an extensive array of resources from the Islamic Art Museum Malaysia (IAMM) and the National Library of Malaysia (NLM) were utilised, alongside personal collections and secondary sources. Data analysis involved employing philological, comparative, deductive, and inductive methodologies. Through this approach, it was revealed that the production of Malay Holy Qur'an's manuscripts has been significantly influenced by local creative input and intellectual contributions from regions such as Turkey, China, and Persia. The study particularly accentuates the diverse calligraphic and illumination techniques across different nations. Notably, manuscripts from the west coast of Peninsular Malaysia, specifically from Malacca and Johor, exhibit distinct artistic qualities that set them apart from those originating from the east coast, including Kelantan and Terengganu. This underscores the prevalence of varied artistic expressions resulting from a broad spectrum of cultural interactions. The development of calligraphy and illumination methods in Malaysian Holy Qur'an's manuscripts mirrors a wide range of artistic expressions, which are shaped by both local innovation and broader Islamic intellectual traditions.