Mushroom compost has been one of the most commonly used fertilisers in organic greenhouse vegetable production in Iceland. However, mushroom compost contains conventional chicken manure. Therefore, the use of this fertiliser is prohibited in organic horticulture. In search of alternative nutrient resources, nine different substitutes for mushroom compost (plant compost, four composted animal manures, fishmeal, white clover residues, coarse meal of faba beans, ‘Pioner complete 6-1-3®’) were tested on two soils differing in organic matter in a pot experiment. Dry matter yield (DM) and the apparent N utilisation with the model plant perennial ryegrass was quantified in five cuts throughout 131 days. Both the DM yield and the apparent N utilisation was influenced by the fertiliser, the soil and their interaction. The N release of the tested alternative organic fertiliser resources was described by the N content and C:N ratio of the fertilisers, but was also affected by the soil. It was concluded that the composted animal manures provided an N release equal to mushroom compost and could therefore act as substitutes.